共查询到19条相似文献,搜索用时 93 毫秒
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本文建立了小鼠灌胃染料木素单体后血浆中水溶性染料木素代谢产物和染料木素脂肪酸酯的分离及测定方法。血浆样品经乙酸乙酯萃取后上Sephadex LH-20柱,分别用体积比1∶1的正己烷/氯仿和甲醇洗脱,染料木素脂肪酸酯用脂肪酶酶解后转化成染料木素,水溶性代谢产物用葡萄糖醛酸酶及硫酸酯酶水解成染料木素,然后采用液相色谱串联飞行时间质谱(Q-TOF LC/MS)检测染料木素。血浆中染料木素在10~10000 ng/mL范围内线性关系良好,检测限为1 ng/mL。10批次小鼠血浆中水溶性代谢产物平均为526.006 ng/mL,染料木素脂肪酸酯平均为58.976 ng/mL。采用SephadexLH-20柱具有良好的分离效果,脂肪酶水解染料木素脂肪酸酯稳定、专一性强,用Q-TOF LC/MS检测染料木素快速、灵敏。 相似文献
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在高效硅胶薄层板上分离了牛蒡子中4种2,3-苄基丁内酯型木脂素──牛蒡甙元(I)、牛蒡素B(Ⅱ)、牛蒡酚A(Ⅲ)、牛蒡酚F(Ⅳ)。流动相为二氯甲烷-乙醚-甲醇-己烷(4:1:0.4:1),室温放置,自然显色。薄层扫描法定量,各木脂素的线性范围在0.451~4.608μg,回收率为99.90%~100.90%,并用此法测定了不同产地牛蒡子中木脂素的含量。方法简便、快速,样品用量少。 相似文献
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6-羟基染料木素(6-OHG)和8-羟基染料木素(8-OHG)是染料木素A环上C6或C8位发生羟基化反应生成的衍生物,其分子中有更多酚羟基,同时含有邻二酚羟基结构,这也使它们的生物活性与染料木素相比发生了显著变化。研究表明,6-OHG和8-OHG具有抗氧化、抗诱变、抑制磷酸二酯酶3B活性、抗糖化、保肝、抗黑色素生成作用、改善记忆力等广泛的药理活性。本文将对6-OHG和8-OHG的来源、制备方法和生物活性研究进行综述,以期为两个化合物的开发利用提供参考。 相似文献
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槐角丸是由槐角、地榆、黄芩、枳壳、防风等组成,具有凉血止血,清肠疏风的功效,适于肠风便血,痔疮肿痛 [1]. 相似文献
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以香草扁桃酸为内标物,提出了测定染料木素的定量核磁共振法(qNMR).采用反门控去耦模式采集氢谱,驰豫延迟时间为40 s,扫描次数为32次,采集时间为4.0 s,溶剂为氘代二甲亚砜,以染料木素中化学位移(δ,×10^(-6))6.38的峰为定量峰,香草扁桃酸中δ6.96为内标物信号峰.结果表明,染料木素与内标物香草扁桃酸的质量比在0.83~7.50内,与其定量峰和内标物信号峰的峰面积比值呈线性关系,检出限(3.3s/k)为0.2 gL^(-1).在精密度试验中,测定值的相对标准偏差(n=6)均小于0.35%;稳定性试验显示供试品溶液在避光条件下24 h内稳定.方法用于实际样品的分析,测定结果与国家标准GB/T 23788-2009的一致. 相似文献
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1 引言 研究胃液中胆汁酸的含量对临床化学和胆汁药物的研究均有重要的意义。胃液中胆汁酸含量甚微,这给选择分析方法带来困难。本文对胃液中胆汁酸的水解、提取、分离等方面的条件进行了研究,提出了同时测定四种胆汁酸的薄层扫描法,综合考察了方法的实用性。 相似文献
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Summary A TLC method for the separation of quantitative determination of copper and iron in cotton material is described. The optimal
solvent system is 9:2:1 (v/v), ethanol-nitric acid-hydrochloric acid and the locating reagent is Na-diethyldithiocarbamate.
Regression analysis shows that the most precise results can be obtained for the concentration range of 0.0400–0.0800 mg/ml
of iron and copper. 相似文献
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Aldo Marino Marina Bicchieri Roberta Curini Guiseppe DAscenzo Vincenzo Carunchio Alessandro Pierucci 《Thermochimica Acta》1981,46(3):289-294
An analytical method useful for the quantitative determination of some bile acids is proposed. The analysis is carried out in two steps. The first is based on the thin layer chromatographic (TLC) separation of the bile acids on alumina plates. The second is based on the application of differential scanning calorimetry (DSC), which permits the characterization and determination of the amount of compound contained in each spot. The DSC signal is proportional to the amount of sample present in the spot layer, while the various peaks and peak temperatures are used to identify the separated compound. 相似文献
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The influence of some experimental parameters on the quantitative determination of specific heats by DSC is discussed. Conditions allowing measurement of the specific heat with a maximum relative error of 1.5 % are proposed. The specific heats of NaA zeolite,C p=0.227±0.003 cal.°C?1.g?1, and AgA zeoliteC p=0.205±0.003 cal.°C?1.g?1 have been determined. 相似文献
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This paper describes a convenient and practice method for quantitation of surfactant phospholipids (1,2-dipalmitoyl-3-sn-phosphatidyl choline [DPPC] and 1-palmitayl-2-oleyl-3-sn-phosphatidyl glycerol [POPG]) in a recombinant surfactant lyophile (Venticute) by high-performance, thin-layer chromatography (HPTLC) with video densitometry. DPPC and POPG were extracted from Venticute-lyophile using methanol. Separation from the other active ingredients and excipients was accomplished by HPTLC on silica gel F254 plates with a mixture of chloroform, methanol, glacial acetic acid, and water as development solvent. Postchromatographic derivatization by dipping in copper sulphate/phosphoric acid reagent and subsequent heating shows grey-brown bands on a light blue background. These were detected with the video densitometer in the VIS range, and with scanning densitometry at 365 nm. Linear calibration in a working range of 0.7-1.3 microg DPPC and 0.35-0.65 microg POPG was demonstrated by integrating the area under the peaks. Good results were obtained with recovery experiments. When compared to classical slit scanning densitometry, video densitometry represents a fast alternative to quantitate thin-layer chromatograms in surfactant phospholipid analysis. 相似文献
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Kh. Kh. Khaiitbaev A. Sultan S. S. Ganiev Kh. A. Aslanov 《Chemistry of Natural Compounds》1993,29(5):586-589
The optimum conditions for extracting flavonols fromAlhagi pseudoalhagi has been determined. A method for the UV-spectrometric identification of the total alkaloids has been developed which permits reliable reproducible results to be obtained. The relative error of the determination does not exceed ±1.7%. 相似文献
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Summary The quantitative determination of chlorophyll a and chlorophyll b was by densitometric scanning using cellulose layers. The
chlorophyll content of plants ofPicea abies stressed with an anionic detergent was compared with that of a control group. After two years of treatment the chlorophyll
content had decreased without the appearance of any visible injury. TLC provides a rapid and simple method for monitoring
vegetation damage. 相似文献