沙拉沙星分子印迹聚合物的制备及其吸附特性

以沙拉沙星为模板分子、甲基丙烯酸为功能单体和乙二醇二甲基丙烯酸酯为交联剂合成了分子印迹聚合物 ,并用平衡吸附实验研究了其吸附性能。结果表明 :该聚合物对沙拉沙星有较高的亲和性和选择性 ,解离常数Kd=7.2 6× 10 - 7～ 2 .19× 10 - 5mol L。     

胆固醇分子印迹聚合物的制备及其选择性吸附

随着人们生活水平的逐步提高,因人体内胆固醇过高所产生的疾病呈逐年增加的趋势.近年来的研究表明,胆固醇分子印迹聚合物对胆固醇具有良好的选择性吸附能力.将胆固醇分子印迹聚合物作为胆固醇的识别剂,提取食品中的胆固醇和治疔胆固醇有关的疾病,展现出了良好的应用前景.本文分别从胆固醇分子印迹聚合物的制备方法、识别机理、提高选择性识别途径以及新的印迹方法等方面进行了综述.     

环丙沙星分子印迹聚合物的合成及识别性能研究

采用分子印迹技术合成了以环丙沙星为印迹分子,以甲基丙烯酸和4-乙烯基吡啶同时为功能单体的分子印迹聚合物。运用平衡结合实验研究了印迹聚合物的吸附特性和选择识别能力。Scatchard分析表明,在所研究的浓度范围内,分子印迹聚合物中形成了两类不同的结合位点。底物选择实验表明,这种聚合物对环丙沙星呈现高的选择结合能力。     

孔雀石绿分子印迹聚合物的制备及其吸附

采用本体聚合法制备了孔雀石绿分子印迹聚合物,对功能单体的种类及用量、交联剂用量、模板浓度和聚合时间等参数进行了优化,并通过等温吸附实验,考察聚合物的吸附性能。 结果表明,以α-甲基丙烯酸为功能单体,当孔雀石绿、α-甲基丙烯酸和乙二醇二甲基丙烯酸酯的摩尔比为1∶4∶20时,所合成的聚合物具有最大的吸附容量,印迹因子（α=QMIP/QNIP）可达到3.6,表明合成的印迹聚合物对孔雀石绿有良好的识别和富集能力。     

磁性分子印迹聚合物微球的制备及吸附特性研究

通过分子印迹技术,以磺胺(SNM)为模板分子,甲基丙烯酸(MAA)为功能单体,利用Fe3O4磁性纳米微球制备具有特异性识别磺胺的磁性分子印迹聚合物.通过紫外分光光度法对磁性印迹聚合物的吸附性能进行研究,并对吸附特性进行了探讨.利用等温吸附数据进行Scatchard分析,从而推断出印迹聚合物的最大吸附量Qmax为280....     

呋喃分子印迹聚合物的制备及其吸附特性

呋喃是在食品加热过程中形成的潜在致癌物质。本实验以呋喃为模板,2-甲基呋喃为模拟模板,α-甲基丙烯酸(MAA)为功能单体,甲基丙烯酸乙二醇酯(EGDMA)为交联剂,采用非共价法合成分子印迹聚合物(MIP)。优化制备过程,并研究聚合物的吸附动力学、等温吸附及吸附识别性能。结果表明,MIP以2-甲基呋喃作为模拟模板,模板单体配比为1∶4时对呋喃的印迹效果最佳,最大吸收量QMIP=69.64mg/g,印迹因子α=1.879。此聚合物对2-甲基呋喃与呋喃具有相似吸附特性,α=Q2-甲基呋喃/Q呋喃=1.004。固相萃取实验表明,此MIP对速溶咖啡样品中的呋喃具有显著的特异性吸附作用,可以固相萃取食品饮料中痕量呋喃。     

烯唑醇分子印迹聚合物的合成及其吸附性能研究

以烯唑醇为模板分子,MAA为功能单体,EDMA为交联剂,采用本体聚合法合成分子印迹聚合物。考察了采用不同比例的模板分子与功能单体合成的聚合物对烯唑醇的吸附量,通过静态吸附试验研究了烯唑醇分子印迹聚合物对烯唑醇吸附性能的影响,并进行Scatchard分析。由Scatchard分析可知模板分子烯唑醇与功能单体MAA形成了1类结合位点,结合位点的离解常数KD=0.117mmol/L,最大表观结合量Qmax=38.66μmol/g。     

分子印迹聚合物的制备及其对香草醛的吸附行为

以香草醛为模板分子、甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂,制备了香草醛分子印迹聚合物(MIP);研究了以不同致孔剂合成的MIP在水溶液中对香草醛的吸附行为.结果表明,以乙腈为致孔剂合成的MIP对香草醛具有较高的识别特性,能较简便地用于香草醛的分离和富集.     

溶菌酶分子印迹聚合物膜的制备及其吸附性能

以溶菌酶为模板蛋白质，结合分子印迹技术在硅烷化的基质玻片上制备了溶菌酶分子印迹聚合物膜。实验优化了溶菌酶聚合物膜的印迹体系，考察了溶菌酶分子印迹聚合物膜的吸附平衡时间、最大吸附量、特异识别能力、重复使用性以及对实际样品中溶菌酶的分离情况。结果表明，在最优条件下，制备的分子印迹聚合物膜对溶菌酶具有特异吸附能力，印迹因子为3.0，吸附平衡时间为5 min，吸附行为符合Langmuir吸附模型，理论最大吸附量为42.5 mg/g，实际样品中的吸附量为30 mg/g。且此印迹聚合物膜在重复使用5次后，最大吸附量仅下降了5%，具有良好的重复使用性。该方法为复杂生物样品中目标蛋白质的分离富集提供了一种快速、高效的手段。     

丹参素分子印迹聚合物的制备及吸附性能研究

在硅胶表面接枝乙烯基三乙氧基硅烷得到接枝硅胶,将其与模板分子丹参素、功能单体4-乙烯基吡啶、交联剂乙二醇二甲基丙烯酸酯等,在偶氮二异丁腈引发作用下进行聚合反应,制备丹参素分子印迹聚合物。采用红外光谱、元素分析对分子印迹聚合物进行表征,静态平衡吸附法和Scatchard分析法研究印迹聚合物的吸附特征。结果表明:该印迹化合物存在两种结合位点,它们的离解常数分别为714.29 mg·L-1和467.74 mg·L-1,饱和吸附量分别为9.93 mg·g-1和61.80 mg·g-1,印迹因子分别达1.93和2.19,对丹参素具有较好的选择吸附性能,可用于丹参素的分离和富集。     

异戊巴比妥分子印迹聚合物的合成和识别性能研究

以异戊巴比妥为模板,分别以丙烯酰胺和甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,用本体聚合法制备了分子印迹聚合物(molecularly imprinted polymer,MIP)。考察了以不同功能单体合成的MIP对模板分子和结构类似物的识别能力和选择性。表明以丙烯酰胺为功能单体合成得到的MIP比甲基丙烯酸为单体的聚合物具有更好的再识别性和选择性,前者对异戊巴比妥的饱和吸附量可达到28．58μmol／g．为理论吸附量的40．9％。     

药物利多卡因分子印迹聚合物的制备及识别特性

利多卡因与甲基丙烯酸为功能单体分别在低温(0～4℃)紫外光照、加热条件下制备利多卡因分子印迹聚合物,实验结果通过Scatchard方程分析可知,低温紫外光照下制备的利多卡因MIP对利多卡因具有良好的选择性,并可进一步应用于临床利多卡因药物的分离富集和检测.     

Selective Adsorption and Molecular Recognition by a Molecularly Imprinted Polymer: Kinetic Study and Analysis

This article presents an original work aiming at kinetically surveying the substrate‐selective mechanism from a molecularly imprinted polymer. With Bisphenol A (BPA) as the template, the imprinted polymer was prepared. The result indicates that the imprinted polymer presents a much more complicated sorption toward the template than toward its analogue. The rate constant in the case of the template appears to be a function of coverage degree. There are also significant deviations from the idealized Langmuir model. Related information indicates that these logically can be a result from the induced molecular memory within the binding framework, which makes the polymer capable of selectively adsorbing the imprint species.     

己烯雌酚印迹分子聚合物合成及其在残留分析中的应用

以己烯雌酚（Diethylstilbestrol,DES）为模板分子,α-甲基丙烯酸为功能单体,二甲基丙烯酸乙二醇酯为交联剂,合成己烯雌酚分子印迹聚合物;以该聚合物为填料制成固相萃取小柱,应用于己烯雌酚残留分析的样品前处理,并比较了该固相萃取小柱与C18固相萃取小柱对DES保留行为的差异。通过选择不同浓度的甲醇水溶液,己烯雌酚达到理想的分离纯化效果;对加标鸡肉样进行了含量测定,回收率可达95％以上。     

发光性分子印迹聚合物的合成及其对组胺的识别性能研究

以组胺为模板分子,α-甲基丙烯酸为共功能单体,锌原卟啉为荧光功能单体,乙二醇二甲基丙烯酸酯为交联剂,合成了一种新的发光性分子印迹聚合物.通过振荡吸附后检测悬浊液荧光和柱吸附后检测流出液中组胺紫外光谱的变化,对印迹和非印迹聚合物与组胺的结合特性进行了对比.两种方法的结果一致,表明印迹聚合物对模板分子的识别选择性优于非印迹聚合物.     

核-壳型厚朴酚印迹聚合物的制备及性能研究

以表面修饰功能基团的SiO2微球为基体,以厚朴酚为模板分子,丙烯酰胺为功能单体,丙烯酸乙二醇二甲酯为交联剂,在SiO2微球表面制备对厚朴酚具有较好选择识别能力的核-壳型印迹聚合物.采用红外光谱及扫描电镜等技术表征聚合物的结构及形态.结果表明,该印迹聚合物表面成功制备了壳层厚度约为200nm的均匀印迹层.通过静态吸附、Scatchard分析法以及竞争吸附实验研究了该聚合物的吸附性能和选择性,结果表明,它对厚朴酚形成均一结合位点,离解常数为0.19mg/mL.     

Preparation and Adsorption Characteristics of Cordycepin Molecularly Imprinted Polymers

The separation of a molecularly imprinted polymer for cordycepin was investigated. The synthesis employed cordycepin as the molecular template, alpha-methylacrylic acid as the functional monomer, glycol dimethyl acrylate as the cross-linking agent, azobisisobutyronitrile as the initiator, and tetrahydrofuran as the solvent and pore-foaming agent. The interaction between cordycepin and the functional monomer was investigated by ultraviolet-visible and infrared spectroscopy. The properties of the molecularly imprinted polymer were analyzed by scanning electron microscopy, equilibrium adsorption experiments, and the Scatchard equation. Static adsorption, solid phase extraction, and high-performance liquid chromatography experiments were employed to evaluate the adsorption properties and selective recognition characteristics. The results showed that the molecularly imprinted polymer had specific adsorption with cordycepin, and the maximum absorption capacity was 1920 µg/g. Scatchard analysis suggested that high affinity and low affinity binding sites were present. For the high affinity case, the dissociation constant and apparent maximum numbers of the binding sites were 0.0089 mmol/L and 4.78 µmol/g, respectively. The dissociation constant and apparent numbers of binding sites were 0.035 mmol/L and 6.047 µmol/g for the low affinity sites. Compared with the corresponding nonimprinted polymer, the cordycepin molecularly imprinted polymer exhibited higher adsorption and selectivity for cordycepin than structural analogs.     

Molecularly Imprinted Polymers with Mandelic Acid as Template

In this report, molecularly imprinted polymers (MIPs) with racemic and L-mandelic acid as the templates were synthesized. Several structural analogues were chosen to study the selectivity of the MIPs prepared using 4-vinylpyridine (4-VP) as the monomer (MIP_4-VP). At the same time, the chromatographic behaviors of the MIP using acrylamide (AA) as the monomer (MIP_AA) in two different mobile phases, acetonitrile and hexane/ethyl acetate (1:1, v/v), were investigated and the separation of the enantiomers was tried. The results indicated that MIP_4-VP had great retention with a template comparable to other analogues. However, no difference in the k′ values of the enantiomers was observed, as the interaction between the polymer and the enantiomers was mainly dependent on the acidity, which showed no difference. The MIP_AA showed a more obvious imprinting effect for the template in hexane/ethyl acetate (1:1, v/v) than in acetonitrile. But the separation of the enantiomers was not achieved even in the less polar solvent, though there was an obvious difference between the retention time of L-MA and D-MA on the L-MA imprinted polymer.     

Theoretical Design and Adsorption Properties of Molecularly Imprinted Polymers Obtained from Chloramphenicol and Acrylamide

Molecular simulations are widely used to model molecularly imprinted polymers(MIPs) in order to enhance their adsorption and selectivity. In this study, chloramphenicol(CAP) and acrylamide(AM) were used as the template and functional monomer, respectively, and pentaerythritol triacrylate(PETA), ethylene glycol dimethacrylate (EGDMA), and trimethylolpropane trimethylacrylate(TRIM) were used as cross-linking agents. The ωB97XD/6-31G(d,p) density functional theory method was employed to simulate binding sites, binding energy, the number of hydrogen bonds, the imprinted molar ratio, which produced the most stable complex, and the interaction mechanism. The cross-linking agent was optimized based on the binding energy. The atoms in molecules theory were used to study the nature of the imprinting effects. The theoretical calculations revealed that CAP and AM formed ordered complexes via hydrogen bonding interactions when the molar ratio between CAP and AM was 1:7 using TRIM as the cross-linking agent. The CAP-AM complex(molar ratio 1:7) had the most stable structure, the largest number of hydrogen bonds, and the smallest ∆E. The experimental results indicate that the CAP-MIPs formed perfect microspheres with an average particle size of 314 nm. Scatchard plot analysis showed that the CAP-MIPs had only one type of binding site over the studied concentration ranges. The dissociation equilibrium constant and maximum apparent adsorption capacities were 1887.35 mg/L(5.84 mmol/L) and 155.56 mg/g(0.482 mmol/g), respectively.     

Molecularly Imprinted Polymers for Stir Bar Sorptive Extraction: Synthesis,Characterization, and Application

Stir bar sorptive extraction is widely used for the preconcentration of organic and inorganic compounds due to its simplicity and versatility. However, commercially available devices are based on nonselective sorbents. Molecularly imprinted polymers provide selective recognition abilities from the template employed during their preparation. In this review, strategies employed for recent for molecularly imprinted polymers employed in stir bar sorptive extraction are described. The advantages and disadvantages are described, as well as future trends involving this preconcentration methodology.