Summary: A novel fluorometric saccharide detection strategy has been established by utilizing saccharide‐induced conformational changes in copolymers containing boronic acid and fluorescent units. The polymer chain changes its conformation due to an alteration of the charge state induced by binding with saccharides. The conformational changes are conveniently detected by monitoring excimer to monomer intensity ratio in the fluorescence spectra. Thus, the present strategy would be a promising way for the creation of highly selective and sensitive saccharide‐sensing systems.
Saccharide‐induced conformational change in copolymers containing boronic acid units. 相似文献
Abstract This paper describes the development of a compact, low power, implantable system for in vivo monitoring of oxygen and glucose concentrations. The telemetry-instrumentation system consists of two amperometric sensors: one oxygen and one glucose biosensor and two potentiostats for biasing the sensors, an instrumentation amplifier to subtract and amplify sensor output signals, and a signal transmitter subunit to convert and transmit glucose dependent signal from the sensors to a remote data acquisition system. The system produces a unipolar glucose dependent voltage in the range of 1 to 3.6 V which is converted to a frequency and then transmitted using a frequency-modulated (FM) oscillator. Initial tests were performed on an open model electronic circuit using resistors to simulate sensor outputs in the 10 to 1000 nA range. Further in vitro evaluation of the system was conducted with a compact printed circuit board embedded in silicone elastomer, entirely submerged in buffer solution using actual sensors. The test results indicated satisfactory operation of the system in simulated implantation conditions for seven days. Response curve of transmitted signal vs glucose concentration was obtained. The results of the in vitro evaluation of the telemetry system permits its subcutaneous implantation in an animal model. 相似文献
Thin, phenylboronic acid‐containing polymer coatings are potentially attractive sensory layers for a range of glucose monitoring systems. This contribution presents the synthesis and properties of glucose‐sensitive polymer brushes obtained via surface RAFT polymerization of 3‐methacrylamido phenylboronic acid (MAPBA). This synthetic strategy is attractive since it allows the controlled growth of PMAPBA brushes with film thicknesses of up to 20 nm via direct polymerization of MAPBA without the need for additional post‐polymerization modification or deprotection steps. QCM‐D sensor chips modified with a PMAPBA layer respond with a linear change in the shift of the fundamental resonance frequency over a range of physiologically relevant glucose concentrations and are insensitive toward the presence of fructose, thus validating the potential of these polymer brush films as glucose sensory thin coatings.
