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阐述了高效毛细管电泳电化学检测器(包括电导、电势和安培检测)的研究现状,重点是检测器的研制及接口的制作技术。对各种电化学检测器的应用情况也进行了总结。展望了高效毛细管电泳电化学检测的发展前景。 相似文献
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毛细管电泳高频电导检测器的研制 总被引:42,自引:0,他引:42
设计了一种新的毛细管电泳检测器.根据高频电导滴定原理,将电导电极做成两个金属圆筒套于分离毛细管外,并向两个电极施加高频电压.经毛细管分离后的组分流过两个电极之间时,高频电流发生变化,得到毛细管电泳图.该检测器具有应用范围广、操作简便及重现性好等优点,已初步应用于混合无机离子的测定. 相似文献
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毛细管电泳直立型安培电化学检测器的研究 总被引:8,自引:0,他引:8
报道了一种新的用于毛细管电泳检测的直立式微型安培电化学检测器,以束状碳纤维盘状电极为工作电极,分离并测定了儿茶酚胺类物质,平均柱数为220000,最小检测量达25amol,且结构紧凑,操作简便,易于推广。 相似文献
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高效毛细管电泳—电荷耦合器件检测器联用技术研究 总被引:5,自引:1,他引:5
报道了用电荷耦合检测器等设备组成的高效毛细管电泳多波长荧光检测装置。以荧光素和罗丹明B对其性能进行了评价,得到了具有光谱和时间分辨的三维电泳图,与被测物标准荧光光谱对照,可以直接鉴定被分离的化合物 相似文献
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IntroductionSincethefirstamperometricelectrochemicaldetection(AED)forcapillaryelectrophoresis(CE)wasreportedbyWallingfordandEwing'inl987,AEDtechniquehasemergedasoneofthemostpowerfuldetectionmethodsforCEowingtoitsselectivity,sensitivity,lowcostanditsabilitytodetectlowlevelsofmanybioIogicallyrelevantmoleculeswithoutpriorderivatization2.However,twoproblemsassociatedwiththecombinationofAEDandCEhavehinderedthewidespreaduseofCE-AEDinroutineanalysis.Oneproblemistheinterferenceofhighvoltage(u… 相似文献
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高效毛细管电泳安培检测的进展 总被引:4,自引:4,他引:4
本文对高效毛细管电泳电化学检测方法中的安培检测进行了评述,安培检测具有灵敏度高,选择的特点,安培检测根据毛细管内径的大小有离柱安培检测和柱端安培检测,近年来脉冲安培分析法、化学修饰电极也已被引入毛细管电泳电化学检测。 相似文献
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高效毛细管电泳—激光干涉折射法测定多元醇的研究 总被引:2,自引:3,他引:2
本文应用高效毛细管电泳、激光干涉折射检测法研究了多元醇在四硼酸钠溶液中电泳行为。详细探讨了溶液pH值,四硼酸钠浓度及多元醇的结构对多元醇-硼酸配位离子淌度的影响。确定了高效毛细管电泳直接分离、激光干涉检测多元醇的新方法。 相似文献
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A method based on capillary electrophoresis with electrochemical detection has been developed for the separa-tion and determination of epicatechin,kaempferol,chlorogenic acid,4-hydroxybenzoic acid,quercetin and proto-catechuic acid in hawthorn for the first time.The effects of working electrode potential,pH and concentration ofrunning buffer,separation voltage and injection time on CE-ED were investigated.Under the optimum conditions,the analytes could be separated in a 60 mmol·L~(-1) borate buffer(pH 8.7)within 21 min.A 300 μm diameter carbondisk electrode has a good response at 0.95 V(vs.SCE)for all analytes.The response was linear over three ordersof magnitude with detection limits(S/N=3)ranging from 3×10~(-8) to 2×10~(-7) g·mL~(-1) for the analytes.The methodhas been successfully applied to the analysis of real sample,with satisfactory results. 相似文献
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应用毛细管电泳-电化学检测法对利尿剂氢氯噻嗪和氨苯喋啶进行了研究。考察了电化学检测和电泳分离条件对氢氯噻嗪和氨苯喋啶分离、检测的影响,结果表明在最佳分离、检测条件下,两种待测物在8 min内达到基线分离。氨苯喋啶和氢氯噻嗪的检测限分别达到0.29和0.25 mg/L。对两物质于日内和日间重复测定7次,迁移时间的日内相对标准偏差(RSD)不大于1.6%,峰电流的日内RSD不大于 3.1%; 迁移时间的日间RSD不大于1.7%,峰电流的日间RSD不大于 4.9%。 将该方法用于复方氨苯喋啶成药中氨苯喋啶和氢氯噻嗪的分离和测定,成药的检测结果与标示量比较,相对误差小于4.6%。在模拟尿样中对氢氯噻嗪和氨苯喋啶进行标准溶液添加回收实验,其回收率分别为93.5%~96.7%和96.6%~97.2%,结果令人满意。 相似文献
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《Electroanalysis》2005,17(13):1187-1191
A disposable electrochemical detector arrangement for capillary electrophoresis has been developed. The detection system consists of an assembly of a fused silica capillary and a microfiber electrode which are fixed in a proper position by sealing this configuration with the help of thermal lamination. The detector design is easy to prepare and inexpensive. Therefore it is used as a disposable device which can be replaced by a new one if any degradation of the performance characteristics occurs. The parameters influencing the detection performance were studied and optimized using nonaqueous capillary electrophoresis and ferrocene derivatives as model compounds. The practical utility was demonstrated by studying the separation and detection of water‐resistant dye compounds. 相似文献
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Electrochemical Detection Using an Engraved Microchip – Capillary Electrophoresis Platform 下载免费PDF全文
The present study describes a simple strategy to integrate electrochemical detection with an assembled microchip‐capillary electrophoresis platform. The electrochemical cell was integrated with a microfluidic device consisting of five plastic squares interconnected with fused silica capillaries, forming a four‐way injection cross between the separation channel and three side‐arms (each of 15 mm in length) acting as buffer/sample reservoirs. The performance of the system was evaluated using electrodes made with either carbon ink, carbon nanotubes, or gold and under different experimental conditions of pH, capillary length, and injection time. Using this system it was possible to separate the neurotransmitters dopamine and cathecol and to quantify phenol from a real sample using a linear calibration curve with a calculated LOD of 0.7 µM. A similar concept was applied to determine glucose, by including a pre‐reactor filled with beads modified with glucose oxidase (GOx). The latter system was used to determine glucose in a commercial sample, with a recovery of 95.2 %. Overall, the presented approach represents a simple, inexpensive, and versatile approach to integrate electrochemical detection with CE separations without requiring access to microfabrication facilities. 相似文献
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将8种人体内必需氨基酸(苏氨酸、缬氨酸、蛋氨酸、异亮氨酸、亮氨酸、苯丙氨酸、赖氨酸、色氨酸)与邻苯二甲醛(OPA)发生衍生化反应,再采用毛细管电泳-电化学检测(CE-ED)方法对其含量进行测定。考察了衍生化时间、检测电位、运行缓冲液浓度和p H值、分离电压及进样时间等的影响。在优化条件下,8种氨基酸在12 min内实现了分离,线性范围为0.1~1 500μg/L;检出限为0.01~0.05μg/L,峰高的相对标准偏差(RSD)为1.3%~1.8%,迁移时间的RSD为0.6%~1.0%。该方法已用于淮山样品中8种必需氨基酸的测定,加标回收率为96.8%~102.0%,RSD均不大于2.4%。 相似文献