共查询到10条相似文献,搜索用时 40 毫秒
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IntroductionSince the first report of transgenic plants ap-peared in1 984[1] ,there has been a rapid progressdirecting toward using this new technique to im-prove crops. Protecting crops from insect pestsquickly became a major goal ofgenetic engineering.By far,the greatest research effort in developingpest- resistant transgenic crops has been devoted tothe expression of Bacillus thuringiensis( Bt) toxinin plants.The formulations based on bacterium Bacillusthuringiensis as a biopesticide for … 相似文献
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ZUBAREV A. Roman 《高等学校化学研究》2010,26(1):17-22
Milk as a key element for infant nutrition represents the only source of feeding for newborns and infants, breast-feeding milk normally contains several bioactive proteins or peptides useful for the development of the immune system that protects infants from diseases. Osteopontin(OPN) plays a distinct role during the processes of lactation. Some studies on OPN isolated and purified from the human milk via HPLC on SCX and C4 columns adopted biological mass spectrometry. After digesting the purified OPN sampl... 相似文献
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Maria Cermeño Thanyaporn Kleekayai Miryam Amigo-Benavent Pádraigín Harnedy-Rothwell Richard J. FitzGerald 《Electrophoresis》2020,41(20):1694-1717
Seaweed (macroalgae) is considered as a sustainable bioresource rich in high-quality nutrients such as protein. Seaweed protein can be used as an alternative to other protein sources. Furthermore, these proteins are natural reservoirs of bioactive peptides (BAPs) associated with various health benefits such as antioxidant, antihypertensive, and antidiabetic activities. However, seaweed-derived BAPs remain underexploited due to challenges that arise during protein extraction from algal biomass. Coupled with this, limited proteomic information exists regarding certain seaweed species. This review highlights the current state of the art of seaweed protein extraction techniques, e.g., liquid, ultrasound, microwave, pulsed electric field, and high hydrostatic pressure assisted extraction. The review also focuses on the enzymatic hydrolysis of seaweed proteins and characterization of the resultant hydrolysates/peptides using electrophoretic and chromatographic techniques. This includes reference to methods employed for separation, fractionation, and purification of seaweed BAPs, as well as the methodologies used for identification, e.g., analysis by mass spectrometry. Furthermore, a bioinformatics or in silico approach to aid discovery of seaweed BAPs is discussed herein. Based on the information available to date, it is suggested that further research is required in this area for the development of seaweed BAPs for nutraceutical applications. 相似文献
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建立了高效液相色谱(HPLC)-串联紫外检测法检测有机肥中洛克沙胂的分析方法。选用20g·L-1K2HPO4作为提取剂,于60℃温度下超声提取有机肥中的洛克沙胂,采用MAX固相萃取柱净化。结果表明,采用Symmetry ShieldTMRP 18色谱柱,以含0.1%甲酸的0.05mol·L-1 KH2PO4∶甲醇=95∶5(V/V)溶液作流动相,等度洗脱,紫外266nm检测,洛克沙胂的保留时间短,且可完全避开杂质峰的干扰;其平均加标回收率介于81.14%~82.75%之间,相对标准偏差(RSD)7.0%,检出限为20μg·L-1。方法重现性好,精密度高,操作简便,适用于有机肥中洛克沙胂检测。 相似文献
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Chirality is an essential property of nature. The emergence of chiral material in nanoscale has shown great promise in the investigation of biological interfaces, chiral optical devices, metamaterials and the origin of chirality. In the present study, we focused on fabricating chiral nanoprobes and their applications in living systems. Through precise synthesis or the self‐assembly of various chiral configurations, significant research achievements have been obtained by our group. In this brief introduction, we will discuss the recent progress of chiral nanostructures in the selective recognition of biomolecules, ultrasensitive detection and their interaction with living cells. 相似文献
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Aptamers are short length, single-stranded DNA or RNA affinity molecules which interact with any desired targets such as biomarkers, cells, biological molecules, drugs or chemicals with high sensitivity. They have been extensively employed for medical applications due to having more advantages than the antibodies such as easier preparation and modification, higher stability, lower batch-to-batch variability and cost. Moreover, aptamers can be easily integrated efficiently with sensors, biosensors, actuators and other devices. In this review article, different applications of aptamers for biological and chemical molecules detection within the scope of electrochemical methods were presented with recent studies. In addition, the present status and future perspectives for highly-effective aptasensors for specific and selective analyte detection were discussed. As in stated throughout the review, combining of extraordinary properties of aptamers with the electrochemical-based biosensors could have improved the sensitivity of the assay and reduced limit of detection. 相似文献
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Mirna Ćurković Perica Ivana Šola Lidija Urbas Franci Smrekar Mladen Krajačić 《Journal of chromatography. A》2009,1216(13):2712-2716
A procedure based on BIA Separations CIM DEAE anion-exchange chromatography was developed to separate double-stranded (ds) RNA of hypovirus infecting phytopathogenic fungus Cryphonectria parasitica. Using a linear gradient of 25 mM 4-morpholinepropanesulfonic acid (MOPS), pH 7.0 as a binding buffer, and 25 mM MOPS, 1.5 M NaCl, 0.1 mM EDTA, 15% isopropanol (v/v), pH 7.0 as an elution buffer, hypoviral dsRNA was additionally purified from nucleic acid species present in preparations partially purified by standard CF-11 cellulose chromatography. Moreover, crude phenol/chloroform extracts of the fungal tissue were also applied to monolithic supports and CIM DEAE chromatograms revealed clear evidence for hypoviral presence without CF-11 chromatography, nucleic acid precipitation, and electrophoresis. 相似文献