共查询到19条相似文献,搜索用时 62 毫秒
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采用电化学石英晶体微天平(EQCM)实时表征和定量检测细胞色素c(Cytc).在压电石英晶振表面上自组装巯基十一酸(MUA)单层膜,以盐酸1-乙基-3-(3-二甲基氨基丙基)碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)活化羧基,将Cytc共价固化到电极表面.EQCM实时监测了MUA的自组装和Cytc的固化过程,测定了二者在电极表面的覆盖度和Cytc的固化量.结果表明,Cytc在0.03~3.00μmol/L浓度范围内呈线性变化,检测限可达到1.19×10-9mol/L. 相似文献
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电化学石英晶体微天平及其应用 总被引:5,自引:2,他引:5
本文介绍了EQCM的原理及其应用,探讨了EQCM在生物大分子,纳米技术方面的应用,详细介绍了EQCM在气味检测,金属电沉积、药物分析等方面研究的优点和EQCM的发展前景。 相似文献
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石英晶体微天平的纳米微球质量放大技术及短序列DNA测定 总被引:6,自引:0,他引:6
提出了一种新型质量放大系统用于提高石英晶体微天平检测的灵敏度.将DNA嵌入剂放线菌素D作为导向分子修饰在磁性微球上,通过石英晶体微天平技术识别测定短序列DNA,使最低检测限从6.2×10-8mol/L下降到2.0×10-12mol/L. 相似文献
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硼酸与二醇的脱水缩合反应已被广泛用于检测或富集顺式邻位多羟基化合物. 本文以对巯基苯硼酸(MPBA)自组装修饰石英晶体微天平(QCM)芯片, 基于硼酸根对唾液酸的特殊反应, 建立了检测唾液酸(SA)的新方法. 采用扫描电子显微镜表征了对巯基苯硼酸对石英晶体微天平的功能化修饰过程, 并研究了溶液pH值对芯片表面亲和力的影响. 实验结果表明, 随着pH值的降低, 修饰芯片对唾液酸的结合能力增强, 而对参照物葡萄糖的结合能力则降低. 在pH=4.0的条件下, 该传感器在0.50~5.0 mmol/L范围内对唾液酸有很好的线性响应, 相关系数为0.9950, 检出限为0.15 mmol/L. 此传感器提供了一种简易的唾液酸检测方法, 为实际样品的测定奠定了基础. 相似文献
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电化学石英晶体微天平研究普鲁士蓝修饰电极 总被引:2,自引:0,他引:2
石英晶体徽天平(Quartz Crystal Microbalance,简称QCM)是一种非常灵敏的质量传感器,其检测能力可达ng级。QCM在化学中的早期应用是检测大气中的徽量成分,目前仍较活跃。由于石英压电晶体浸入溶液后在晶体/溶液界面存在较大的能量损失而不能够稳定振荡,致使QCM的应用较长时间局限于气相。八十年代初石英压电晶体在液相中的振荡终获成功,开辟了QCM应用的一个全新领域。液相中振荡成功后,QCM很快应用于电化学研究。目前已发展成为一种全新的电化学传感器——电化学石英晶体微天平(EQCM),并已用于金属电沉积、电化学腐蚀、电分析等方面的研究。 相似文献
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Schafer Medina Adan Tibbits Gretchen Wall Nathalie A. Ivory Cornelius F. Clark Sue B. Beyenal Haluk 《Journal of Radioanalytical and Nuclear Chemistry》2020,324(3):1021-1030
Journal of Radioanalytical and Nuclear Chemistry - Microliter volumes are used in electrochemical detection and preconcentration of radionuclides to reduce the dose received by researchers and... 相似文献
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Yann Ratieuville Philippe Viers Jean Alexandre Grard Durand 《Electrochemistry communications》2000,2(12):2727
As the resonance frequency of an oscillating quartz changes upon the deposition of a given mass to the crystal surface, it can be used as a very sensitive mass measuring device. Despite a growing interest in the use of electrochemical quartz crystal microbalance (EQCM), there is still no commercial available measurement cell which can satisfy all the conditions needed in electrochemical investigations. The design and characteristics of a new electrochemical cell adapted to EQCM measurements are presented. The sensitivity of the microbalance, which is determined by several calibration runs of silver electrodeposition is (183.2±2.9) Hz μg−1 cm2. This value, which is close to the theoretical one, confirms the validity of the system we have developed. The calibration procedure and the EQCM using range are then discussed. 相似文献
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Michał Grdeń Jan Kotowski Andrzej Czerwiński 《Journal of Solid State Electrochemistry》2000,4(5):273-278
The electrochemical quartz crystal microbalance (EQCMB) method has been used to evaluate the processes which occur in/on
the palladium electrode in basic solutions. Hydrogen electrosorption in palladium is accompanied by an additional frequency
shift that can be attributed to the stresses generated inside the Pd metal. A non-linear dependence between the mass change
and the charge consumed during hydrogen oxidation in the Pd electrode has been found for hydrogen absorbed in the α- and β-phases.
This effect precludes the objective estimation of the amount of hydrogen absorbed inside the Pd electrode. The EQCMB method
has been used, however, for studying the surface electrode processes on the Pd electrode, i.e. specific anion adsorption,
surface oxidation and dissolution. Also, the structure of the palladium oxide formed on the Pd surface during electrochemical
oxidation is discussed in this paper and the effect of the anodic limiting potential on the oxide structure is reported.
Received: 10 August 1999 / Accepted: 24 September 1999 相似文献
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《Electrochemistry communications》2001,3(6):274-280
Water and ion transport in electrochemically prepared Prussian blue (PB, Iron(III) hexacyanoferrate(II)) films has been investigated with the electrochemical quartz crystal microbalance (EQCM) and the electrochemical/electrogravimetric impedance techniques. It is shown that the freshly prepared PB film is highly hydrated and that it undergoes an irreversible mass change during the first cathodic scan. The latter result supports the previously proposed structural reorganization scheme of the PB film from the insoluble form Fe4[Fe(CN)6]3·6H2O to the soluble form MFeFe(CN)6 (M is a monovalent cation). It is also shown that, during the first cathodic scan, a substantial amount of water is excluded from the PB film. After the structural reorganization, ion transport during the redox reaction of the PB film is cation-dominant with a small fraction of accompanying water transport. 相似文献
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《Electrochemistry communications》2000,2(10):692-696
The changes observed on Pt surfaces during a potential incursion into the underpotentially deposited (UPD) hydrogen and double layer ranges (0.05 to 0.8 V versus HESS) were analyzed in perchloric, sulfuric, phosphoric and hydrochloric acid media. Surface occupation after hydrogen desorption was verified in terms of both mass variations and voltammetric charges measured by EQCM and cyclic voltammetry. Firstly, mass incorporation due to the adsorption of water molecules (approximately 39 ng cm−2, corresponding to a full monolayer of adsorbed water) replacing the UPD H atoms was observed in every case. The potential range associated with water adsorption varied from 0.05 V to a final value that depended on the strength of anion adsorption on Pt (0.4 V for ClO4− and 0.3 V for Cl−). Secondly, the mass incorporations in the potential region between 0.4 and 0.8 V were associated to adsorption of the corresponding hydrated anions, i.e., ClO4−·2H2O, HSO4−·2H2O, HPO42− and Cl−·6H2O. Calculated anion coverage values varied from 7 (perchlorate) to 19% (phosphate) on the Pt surface. 相似文献
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Francisco Vicente Joan Gregori Jose Juan García-Jareño David Giménez-Romero 《Journal of Solid State Electrochemistry》2005,9(10):684-690
The electrochemical quartz crystal microbalance results show that nickel electrodissolution and nickel passivation occur simultaneously. Besides, the anodic transferred charge decreases and the passive layer thickness increases with the number of successive voltammetric cycles. Mass balances across the metal/passive layer/solution have been done from the instantaneous F(dm/dQ) function. A dynamic process for generation of an inner NiO and a Ni(OH)2 outer passive layers is suggested. 相似文献
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This work describes the results of a mechanistic investigation of antibody-antigen binding using electrochemical quartz crystal microbalance (EQCM). The aim was to verify the contribution of electrolytes to conducting polypyrrole electrodes that have been modified with proteins. The behavior of an EQCM film containing various counterions was studied (chloride, dodecylsulphate and proteins) and mass changes recorded in a series of anions, cations and proteins. Results obtained indicate that the interaction of different proteins at quartz crystal electrode surface is dependent on the applied potential, the nature of the cations and anions, and the specificity of the immobilized antibody. The resonant frequency of the anti-HSA-coated quartz abruptly decreased upon contact with the antigen and this stabilized within 5 min in the concentration range between 1 and 100 ppm. The injection of other proteins such as bovine serum albumin and chymotrypsin, yielded responses that were significantly lower in magnitude than those obtained for the corresponding HSA. 相似文献