Intramolecular synergism,an explanation for the enhanced fungitoxicity of halo-8-quinolinols

Summary An antifungal study againstAspergillus niger,A. oryzae,Myrothecium verrucaria, andTrichoderma viride in Yeast Nitrogen Base supplemented with 1%D-glucose and 0.088%L-asparagine was carried out using 8-quinolinol and 3-, 5-, 6-, 7-, 3,6-, and 5,7-chlorinated and brominated-8-quinolinols. Binary mixtures of 3- and 6-halo- and 5- and 7-halo-8-quinolinols were intermolecularly synergistic. MICs of the monohalo synergistic mixtures admixed with a MIC of the corresponding dihalo-8-quinolinols were not synergistic. The dihalo-8-quinolinols with substituents in positions corresponding to those of the synergistic binary mixtures appeared to attack the same sites of action as the binary pairs. The enhanced activities of 3,6- and 5,7-dichloro-8-quinolinols and 3,6- and 5,7-dibromo-8-quinolinols are believed to be due to intramolecular synergism. The greater fungitoxicity of 5-, 6-, and 7-monohalo-8-quinolinols over 8-quinolinol can also be explained as due to intramolecular synergism. 3,6-Dihalo- and 5,7-dihalo-8-quinolinols formed synergistic pairs of compounds.

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Intramolekularer Synergismus als Erklärung für die erhöhte Fungitoxizität von halogenierten 8-Chinolinolen

Zusammenfassung 8-Chinolinol und verschiedene halogenierte 8-Chinolinole wurden auf ihre Fungitoxizität gegenüberAspergillus niger,A. oryzae,Myrothecium verrucaria undTrichoderma viride untersucht. Binäre Mischungen von 3- und 6-Halogen- bzw. 5- und 7-Halogen-8-chinolinol zeigten intermolekularen Synergismus, während bei Mischungen von mono- und dihalogenierten 8-Chinolinolen kein entsprechender Effekt beobachtet werden konnte. Die erhöhte Aktivität von 3,6-und 5,7-Dichlor-8-chinolinol und 3,6- und 5,7-Dibrom-8-chinolinol wird durch intramolekularen Synergismus erklärt, desgleichen die höhere Aktivität monohalogenierter 8-Chinolinole gegenüber 8-Chinolinol. 3,6-Dihalogenierte und 5,7-dihalogenierte 8-Chinolinole bilden synergistische Paare.

     

Evidence of steric factors in the fungitoxic mechanisms of 8-quinolinol and its 2-, 3-, 4-, 5-, 6-and 7-chloro and bromo analogues

Summary A study was made of the fungitoxicity of 2-, 3-, 4-, 5-, 6- and 7-chloro and bromo-8-quinolinols againstAspergillus niger,A. oryzae,Myrothecium verrucaria,Trichoderma viride andTrichophyton mentagrophytes in Sabouraud dextrose broth and in Yeast Nitrogen Base supplemented with 1%D-glucose and 0.088%L-asparagine. Based on the presence or absence of synergism between pairs of substituted 8-quinolinols and reversal or nonreversal of toxicity byL-cysteine or N-acetyl-L-cysteine, the following conclusions were reached: (1) substituents on the quinoline ring change the site(s) of action of the toxicant; (2) the sites of action of the 5-, 6-, and 7-chloro-8-quinolinols are different from each other and from 8-quinolinol and its 2-, 3-, and 4-chloro analogues, and the same is true for the corresponding bromo compounds; (3) 8-quinolinol and its 3- and 4-chloro and bromo analogues appear to share common sites of action; (4) for good antifungal activity the 2 position of the ring must not be substituted by sterically bulky groups; (5) the geometry of the binding sites of action are not so constrained that they cannot accommodate the analogously substituted chloro- and bromo-8-quinolinols.

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Nachweis sterischer Faktoren bei der Fungitoxizität von 8-Chinolinol und seinen 2-, 3-, 4-, 5-, 6- und 7-Chlor- und -Brom-Analogen

Zusammenfassung Es wurde eine Studie der Fungitoxizität von 2-, 3-, 4-, 5-, 6- und 7-Chlor- und-Brom-8-chinolinol gegenüberAspergillus niger,A. oryzae,Myrothecium verrucaria,Trichoderma viride undTrichphyton mentagrophytes in Sabouraud Dextrose Nährmedium und in Hefe-N-Base mit 1%D-Glucose und 0.088%L-Asparagin unternommen. Auf der Basis des Zutreffens oder der Abwesenheit eines Synergismus zwischen Paaren von substituierten 8-Chinolinolen und der Umkehrung oder Nichtumkehrung der Toxizität durchL-Cystein oder N-Acetyl-L-cystein wurden folgende Schlußfolgerungen abgeleitet: (1) Substituenten am Chinolin-Ring ändern die Aktionsstelle(n) des Toxikans; (2) Die Angriffsstellen der 5-, 6- und 7-Chlor-8-chinolinole sind untereinander und von 8-Chinolinol und seinen 2-, 3- und 4-Chlor-Analogen verschieden, wobei das auch für die entsprechenden Brom-Verbindungen gilt; (3) 8-Chinolinol und seine 3- und 4-Chlor- und -Brom-Analogen scheinen gemeinsame Aktionsstellen zu teilen; (4) für eine gute antifungale Aktivität darf die 2-Position des Rings nicht mit sterisch anspruchsvollen Gruppen besetzt sein; (5) Die Geometrie des Bindungsstellen der Wirkung ist nicht so gespannt, daß nicht sowohl analoge Chlor- oder Brom-8-chinolinole Platz finden.

     

Preparation and Fungitoxicity of Some Trichloro-, Tribromo-, Tetrachloro-, and Tetrabromo-8-Quinolinols

Summary.  3,5,6-, 3,5,7-, 4,5,7-, and 5,6,7-trichloro- and -tribromo-8-quinolinols as well as 3,5,6,7-tetrachloro- and -tetrabromo-8-quinolinols were prepared and tested against six fungi (Aspergillus niger, Aspergillus oryzae, Myrothecium verrucaria, Trichoderma viride, Mucor cirinelloides, and Trichophyton mentagrophytes) in Sabouraud dextrose broth. The compounds strongly inhibit five fungi but not M. cirinelloides. They are less active than the related dichloro-8-quinolinols which is attributed to steric hindrance. Received April 3, 2001. Accepted April 10, 2001     

O-Alkylations of pyridoxine and pyridoxamine

O-Alkylations of pyridoxine 1 and pyridoxamine 5 were carried out in acetone in the presence of sodium ethoxide and potassium iodide to give 5-alkoxy-6-methyl-3,4-(bishydroxymethyl)pyridines 2a-j and 5-alkoxy-4-aminomethyl-6-methyl-3-hydroxyrnethylpyridines 6a-e .     

Synthesis and Reactivity of 5-Alkoxybenzo[b]furan-2-selenolates

By reaction of selenium(IV) oxide with 5-alkoxy-2-hydroxyacetophenone semicarbazones4-(5-alkoxy-2-hydroxyphenyl)-1'2'3-selenadiazoles were prepared. The latter readily decomposed whentreated with potassium carbonate yielding 5-alkoxybenzo[b]furan-2-selenolates. The selenolates obtainedunderwent alkylation effected by monochloroacetamide, were arylated by 2'4-dinitrochlorobenzene. Theoxidation of selenolates with iodine furnished bis(5-alkoxybenzo[b]furan-2-yl) diselenides.     

N-(2-Pyridyl)amides of 2,4-Dioxobutyric Acids in Reactions with Diazoalkanes

N-(2-Pyridyl)amides of 4-R-2-alkoxy-4-arylcrotonic acids and 3-alkoxy-3-aroylmethyl-2-oxo-2,3-dihydroimidazo[1,2-a]pyridines have been synthesized by the interaction of N-(2-pyridyl)amides of 4-aryl-2,4-dioxobutyric acids with diazoalkanes. The structure and mechanism of formation of the products are discussed.     

Preparation and Fungitoxicity of Some Trichloro-, Tribromo-, Tetrachloro-, and Tetrabromo-8-Quinolinols

 3,5,6-, 3,5,7-, 4,5,7-, and 5,6,7-trichloro- and -tribromo-8-quinolinols as well as 3,5,6,7-tetrachloro- and -tetrabromo-8-quinolinols were prepared and tested against six fungi (Aspergillus niger, Aspergillus oryzae, Myrothecium verrucaria, Trichoderma viride, Mucor cirinelloides, and Trichophyton mentagrophytes) in Sabouraud dextrose broth. The compounds strongly inhibit five fungi but not M. cirinelloides. They are less active than the related dichloro-8-quinolinols which is attributed to steric hindrance.     

Synthesis of 2-alkoxy-6-aryl-5-cyano-4(3H)-pyrimidinones

The reaction of methyl 3-aryl-2-cyano-3-methoxypropenoates 1 with cyanamide and sodium methoxide in methanol afforded after acid hydrolysis the methyl 3-[(aminocarbonyl)amino]-3-aryl-2-cyanopropenoates 4 . By performing the reaction in higher boiling alcohols the 2-alkoxy-6-aryl-5-cyano-4(3H)-pyrimidinones 2 were obtained in good yields.     

Facile stereoselective synthesis of cis- and trans-3-alkoxyazetidin-2-ones

A highly stereoselective synthesis of cis- and trans-3-alkoxy-3-phenyl/benzylthioazetidin-2-ones is described. The reaction of α-chlorosulfide-β-lactams with various alcohols catalyzed by a Lewis acid such as ZnCl₂ in the presence of molecular sieves (3-4 Å) leads to cis-3-alkoxy-3-phenyl/benzylthio-β-lactams whereas treatment of potassium 2-alkoxy-2-phenylthioethanoate with appropriate Schiff's base using POCl₃ in the presence of triethylamine leads to the formation of trans-3-alkoxy-3-phenylthioazetidin-2-ones as major products.     

Haloacetylated enol ethers. 2. Synthesis of 5-trifluoromethylpyrazoles

1-Methyl-5-(trifluoromethyl)-1H-pyrazoles 2, 3 and 4,5-dihydro-1-phenyl-5-(trifluoromethyl)-1H-pyrazol-5-ol 4 were prepared by reaction of 4-alkoxy-1,1,1-trifluoro-3-alken-2-ones 1 and hydrazine, methylhydrazine, and phenylhydrazine, respectively, in good yields. Compound 1 proved to be a versatile building block for the regiospecific construction of pyrazole rings having an 5-trifluoromethyl substituent.     

Synthesis of certain tricyclic quinoline derivatives as potential antiamebal agents

Synthesis of the fused tricyclic systems, 1, 2, 3, 3a, 4, 5-hexahydropyrrolo[1, 2a]quinoline (I) and 2, 3, 4, 4a, 5, 6-hexahydro 1H-benzo[c]quinolizine (II), each bearing an ethoxyl substituent on the benzene ring, has been achieved. The compounds combine certain structural features of the alkaloid emetine and of 8-quinolinols.     

Preparation of 6-Fluoro-8-quinolinol and Related 6-Fluoroquinolines

 6-Fluoro-8-quinolinol was prepared from 2-amino-5-fluorophenol by a Skraup synthesis. No synergism was observed between 5-fluoro- and 6-fluoro-8-quinolinols or between 6-fluoro- and 7-fluoro-8-quinolinols against any of the six fungi in our test system (Aspergillus niger, A. oryzae, Myrothecium verrucaria, Trichoderma viride, Mucor cirinelloides, and Trichophyton mentagrophytes) in Sabouraud dextrose broth. Unlike the fluoro-8-quinolinols, the 8-quinolinols comparably substituted with chlorine or bromine did form synergistic mixtures. This is attributed to steric factors.     

Synthesis of 5-alkoxy-3-amino-7-nitro-1,2,4-benzotriazine 1-oxides from 1,3,5-trinitrobenzene

1-Alkoxy-3,5-dinitrobenzenes were nitrated to give 1-alkoxy-2,3,5-trinitrobenzenes. The reaction of the latter with guanidine affordsN-(2-alkoxy-4,6-dinitrophenyl)guanidines, which undergo cyclization under the action of KOH to form 5-alkoxy-3-amino-7-nitro-1,2,4-benzotriazine 1-oxides. Published inIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 9, pp. 1650–1652, September, 2000.     

Synthesis of 2-[3-alkoxy-4-(hydroxy,alkoxy, acyloxy)phenyl]-5-methyl-1<Emphasis Type="Italic">H</Emphasis>-benzimidazoles

From substituted benzaldehydes of the vanillin series and 4-methyl-1,2-phenylene diaminein the presence of sodium hydrogen sulfite in DMF medium at 80°C new functionally substituted 2-[3-alkoxy-4-(hydroxy, alkoxy, acyloxy)phenyl]-5-methyl-1H-benzimidazoles were obtained in preparative yields.     

<Emphasis Type="Italic">N</Emphasis>-[(<Emphasis Type="Italic">E</Emphasis>)-3-alkoxy-4-hydroxy(alkoxy,alkanoyloxy, aroyloxy)-benzylidene]-4-phenoxyanilines

Condensation of 4-phenoxyaniline with benzaldehydes of the vanillin series in methanol gave the corresponding Schiff bases, N-[(E)-3-alkoxy-4-hydroxy(alkoxy, alkanoyloxy, aroyloxy)benzylidene]-4-phenoxyanilines.     

Preparation of 6-Fluoro-8-quinolinol and Related 6-Fluoroquinolines

Summary.  6-Fluoro-8-quinolinol was prepared from 2-amino-5-fluorophenol by a Skraup synthesis. No synergism was observed between 5-fluoro- and 6-fluoro-8-quinolinols or between 6-fluoro- and 7-fluoro-8-quinolinols against any of the six fungi in our test system (Aspergillus niger, A. oryzae, Myrothecium verrucaria, Trichoderma viride, Mucor cirinelloides, and Trichophyton mentagrophytes) in Sabouraud dextrose broth. Unlike the fluoro-8-quinolinols, the 8-quinolinols comparably substituted with chlorine or bromine did form synergistic mixtures. This is attributed to steric factors. Corresponding author. E-mail: clarke@fordham.edu Received May 23, 2002; accepted May 29, 2002     

Stereoselective synthesis of Z-3-alkoxy-2-[(4′-methoxyphenyl)methylidene]-1(3H)-isobenzofuranones

Photoreorganisation of 2-alkoxy-2-(4′-methoxyphenyl)-1H-indene-1,3(2H)-diones in anhydrous acetone affords exclusively Z-3-alkoxy-3-[(4′-methoxyphenyl)methylidene]-1(3H)-isobenzofuranones. The products were identified by X-ray crystallography of one of them.     

Synthesis of 6-substituted 1-alkoxy-5-alkyluracils

Synthesis of 6-substituted 1-alkoxy-5-alkyluracils 2a-c have been achieved from readily accessible 2-alkyl-3,3-di(methylthio)acryloyl chlorides 4a,b in high overall yields. Treatment of 4a,b with silver cyanate followed by reaction of the resulting isocyanates 5a,b with an appropriate alkoxyamine afforded N-alkoxy-N′-[2-alkyl-3,3-di(methylthio)acryloyl]ureas 6a,b in 85–88% yields. Cyclization of 6a,b in acetic acid containing methanesulfonic acid followed by oxidation with 3-chloroperoxybenzoic acid gave high yields of 1-alkoxy-5-alkyl-6-(methylsulfonyl)uracils 9a,b. Nucleophillic addition-elimination reaction of 9a,b with sodium azide, phenylthiol, or phenylselenol produced 6-azido-1-butoxythymine ( 2a , 98%), 5-ethyl-1-(2-phenoxyethoxy)-6-(phenylthio)uracil ( 2b , 95%), or 5-ethyl-1-(2-phenoxyethoxy)-6-(phenylselenenyl)uracil ( 2c , 91%).     

Effect of Dimethyl Sulfoxide and Dimethylformamide on the Stability of 4-Halo-8-quinolinols

Summary. Polyhalo-8-quinolinols with chlorine or bromine in position 4 were not stable in DMSO or DMF. The degradation product from 4,5-dichloro-8-quinolinol was 5-chloro-4,8-quinolindiol and the major product from 4,5-dibromo-8-quinolinol was 3,5-dibromo-4,8-quinolindiol. 4,5,7-Trichloro- and 4,5,7-tribromo-8-quinolinols yielded similar hydrolytic products, and for the bromo compound, a rebrominated product in DMSO. In DMF rebromination did not occur. In pyridine-d₅ these reactions did not take place, indicating a special ability of DMSO and DMF to cause such hydrolysis at position 4 of 4-halo-8-quinolinols.Received December 13, 2002; accepted January 7, 2003 Published online June 23, 2003     

Preparative synthesis of 2-[3-alkoxy-4-(hydroxy,alkoxy, acyloxy)phenyl]-1H-benzimidazoles proceeding from substituted benzaldehydes

New functionally-substituted 2-[3-alkoxy-4-(hydroxy, alkoxy, acyloxy)phenyl]-1H-benzimidazoles were synthesized in preparative yields from aldehydes of vanillin series, their ethers and esters by the reaction with 1,2-phenylenediamine in the presence of sodium hydrogen sulfite in DMF solution at 80°C.