用全反射方法测量离体动物组织的折射率

利用全反射原理测量了新鲜离体动物组织在650 nm处的折射率.选择了鸡胸肉、猪肌肉、牛肌肉、羊肉、猪肝脏和猪肾脏六种类型的组织作为研究对象,将其制成2 mm厚的切片进行测量,折射率的测量结果范围在1.384 52~1.418 26.特别是对其中肌肉纤维取向明显的鸡胸肉、猪肌肉、牛肌肉,分别测量了肌肉纤维在平行和垂直棱镜与生物组织界面两种情况下的折射率.结果表明,测量的肌肉纤维垂直界面时的折射率要大于平行时的结果.     

基于无芯光纤的多参数测量传感器

设计并制作了一种基于单模-无芯-单模-无芯-单模光纤结构的马赫-曾德尔传感器,可用来同时测量折射率和温度.该传感器中,两处无芯光纤充当输入、输出耦合器,中间单模光纤作为传感臂.利用有限元仿真和理论分析,确定耦合器和传感臂的最优长度为15 mm.在无芯光纤中激发出的高阶模进入单模光纤的包层传输,由于倏逝场的作用,受到环境折射率和温度的影响.选取透射谱不同干涉级次的波谷作为研究对象,实现了折射率和温度的同步测量.实验结果表明:1545 nm附近干涉谷的折射率和温度灵敏度分别为–153.89 nm/RIU(refractive index unit)和0.166 nm/℃;1570 nm附近干涉谷的折射率和温度灵敏度分别为–202.74 nm/RIU和0.183 nm/℃.该传感器在实现折射率和温度同步测量的同时,仍能保持较高灵敏度,在生物医疗等方面有着较好的应用前景.     

用毛细管焦点法精确测量微量液体的折射率

介绍了用毛细管焦点法精确测最微量液体折射率的一种新技术.该技术基于共轴球面光学系统的成像原理,用LED(λ=580 nm,FWHM为32nm)为测量光源,用CCD为像接收装置.一次测量样品需要量小于0.002 mL.待测样品封闭在毛细管内测量,有利于对毒性、挥发性和吸湿性强的液体介质折射率的测量.用此技术对纯水、乙醇、乙二醇和丙三醇样品的折射率做了测量,测量精度分别为0.0001,0.0002,0.0003和0.0003.论文在分析实验装置的测量灵敏度和成像景深基础上,提出了进一步提高测最精度的方法.     

基于光纤M-Z干涉的高灵敏度液体折射率传感器的实验研究

提出并制备了一种基于Mach-Zehnder干涉效应的高灵敏度光纤液体折射率传感器.分别利用NaCl溶液和甘油溶液,研究了传感器的透射光谱和外界介质折射率的关系.实验结果表明,随着周围介质折射率的增大,传感器干涉谱的极小值点对应的波长向长波方向漂移,在1.333～1.356的折射率变化范围内,极小值点对应的波长的漂移量和折射率的变化具有较好的线性关系,对应的灵敏度约为4 086nm/refractive-index.该传感器制作简单、结构紧凑,在生物和化学测量中具有较好的应用前景.     

基于光纤M-Z干涉的高灵敏度液体折射率传感器的实验研究

提出并制备了一种基于Mach-Zehnder干涉效应的高灵敏度光纤液体折射率传感器.分别利用NaCl溶液和甘油溶液,研究了传感器的透射光谱和外界介质折射率的关系.实验结果表明,随着周围介质折射率的增大,传感器干涉谱的极小值点对应的波长向长波方向漂移,在1.333~1.356的折射率变化范围内,极小值点对应的波长的漂移量和折射率的变化具有较好的线性关系,对应的灵敏度约为4 086 nm/refractive-index.该传感器制作简单、结构紧凑,在生物和化学测量中具有较好的应用前景.     

用液芯柱透镜测量液体折射率

制作了一种液芯变焦柱透镜并将其用于精确测量液体的折射率.在空心的柱透镜内注入待测折射率的液体,通过测量液芯透镜的焦距,根据焦距和折射率之间的关系,计算出液芯介质的折射率.此方法有效地提高了折射率测量的灵敏度,减小了测量系统的景深.选取×20倍显微物镜可实现液体折射率1.3～1.642范围内的精确测量,单次测量误差小于0.000 2.     

基于多模光纤偏芯熔接实现温度和折射率同时测量的光纤传感器

利用偏芯熔接的方法,研制出一种可实现温度和折射率同时测量的光纤传感器。该传感器将一段多模光纤MMF2的左端与一芯径与长度均相同的多模光纤MMF1偏芯熔接,右端与一大芯径多模光纤MMF3对芯熔接构成传感头,利用多模光纤纤芯模和包层模对温度、折射率的敏感性差异,结合敏感矩阵实现了双参量同时测量。实验选取了位于1536.98nm和1545.24nm处的干涉谷进行了温度和折射率的测量,测得1536.98nm处的干涉谷对温度的灵敏度为0.105nm/℃,对折射率不敏感;1545.24nm处的干涉谷对温度的灵敏度为0.052nm/℃,对折射率的敏感性为32.2nm/RIU(RIU表示单位折射率)。该传感器也可应用于其他参量的测量,具有良好的应用前景。     

芯内双微孔复合腔结构的光纤法布里-珀罗传感器研究

提出了一种基于芯内双微孔复合结构的全光纤干涉传感器结构,建立了传感器反射光谱的理论模型,给出了反射光谱强度与微孔长度、孔内介质折射率、微孔端面反射与损耗系数以及光纤的特性参数间的关系,并模拟了传感器光谱对温度和折射率变化的响应特性.利用193 nm准分子激光器,在普通单模光纤上加工制作了具有复合腔结构的全光纤多参量传感器,进行了传感实验研究.结果表明,该传感器具有优于99%的温度、折射率线性响应度,对应两套温度和折射率灵敏度分别为-0.172 nm/℃,1050.700 nm/RIU和0.004 nm/℃,48.775 nm/RIU,不仅能够实现温度、折射率以及它们的区分测量,还能够应用于气体压力的测量,测量精度可达0.3 kPa.     

功能型液体折射率与温度双参量光纤传感教学实验

设计了光电专业光纤传感单参量测量向双参量升级的简易实验方案,采用宽带掺铒光纤放大器作为光源,光纤双锥马赫-曾德尔干涉仪结合光纤布拉格光栅作为液体折射率与温度双参量传感器,光谱仪测量特征波长变化用于待测量解调.经测试,功能型液体折射率与温度双参量光纤传感系统的折射率传感灵敏度为-34.663nm/RIU,温度传感灵敏度为10pm/℃.     

基于光学外差干涉的折射率测量方法

基于光学折射率是材料的一个重要的物理参数,提出了一种在泰曼格林干涉系统基础上的光学外差干涉折射率测量方法及系统。测量系统将样品放置在系统外,采用双光束干涉探测,使系统更加稳定、调整简单、测量更加精准。实验上测量了K9玻璃块样品在超连续激光光源中心波长790nm、带宽20nm下的折射率,验证了方法的有效性和可靠性。实验结果表明,系统能够准确测量出样品的折射率,测量稳定性较高,精度可以达到10-4;适用于可见光和近红外波段透明物质的折射率测量,应用领域广泛。     

Precise measurement of group refractive indices and temperature dependence of refractive index for Nd-doped yttrium orthovanadate by intracavity spontaneous mode locking

We report on a novel method based on intracavity spontaneous mode locking to precisely measure the group refractive indices and temperature dependence of refractive index of Nd:YVO(4) crystal at the wavelength of 1064 nm. All the experimental results are found to agree very well with the most recent measured values. We also confirm that the developed method is applicable to measuring the group refractive indices and the temperature dependence of the refractive indices of other vanadate crystals, as well as nonlinear crystals.     

Measurement of optical penetration depth and refractive index of human tissue

Experimental techniques for measurement of optical penetration depth and refractive index of humantissue are presented, respectively. Optical penetration depth can be obtained from the measurement ofthe relative fluence-depth distribution inside the target tissue. The depth of normal and carcinomatoushuman lung tissues irradiated with the wavelengths of 406.7, 632.8 and 674.4 nm in vitro are respectivelydetermined.In addition,a novel simple method based on total internal reflection for measuring therefractive index of biotissue in vivo is developed,and the refractive indices of skin from people of differentage, sex and skin color are measured.Their refractive indices are almost same and the average is 1.533.     

A proposed new experimental setup for determination of refractive indices of liquids by mode line measurements

A new experimental method is proposed for measuring the refractive indices of liquids based on mode line measurements. The experimental setup consists of a dielectric substrate in which a rectangular hollow is formed containing the concerned liquid. This is covered by a trapezium-shaped prism. The liquid, confined between both basin walls and the prism, forms a wavegude. Thus, the refractive index of the liquid can be determined by measuring the propagating modes in the structure. Using this technique, the refractive indices of liquids can be measured to an accuracy of the order of 1×10^-4.     

Optical absorption and scattering spectra of pathological stomach tissues

Diffuse reflection spectra of biotissues in vivo and transmission and reflection coefficients for biotissues in vitro are measured over 300–800 nm. These data are used to determine the spectral absorption and scattering indices and the scattering anisotropy factor for stomach mucous membranes under normal and various pathological conditions (chronic atrophic and ulcerous defects, malignant neoplasms). The most importan tphysiological (hemodynamic and oxygenation levels) and structural-morphological (scatterer size and density) parameters are also determined. The results of a morphofunctional study correlate well with the optical properties and are consistent with data from a histomorphological analysis of the corresponding tissues.     

Control of optical properties of biotissues: I. spectral properties of the eye sclera

A method for computer modeling of transmission and diffuse reflection of a strongly scattering biotissue is described. Results of calculations of spectral characteristics for a model of the human eye sclera are presented and compared with experiments. The possibility to control optical properties of biotissues is theoretically substantiated and experimentally confirmed. Variations in color indices of biotissues are calculated using theoretical and experimental spectral characteristics obtained for the eye sclera.     

Brillouin scattering measurement for refractive indices of anisotropic crystals

An experimental method is proposed for measuring the refractive indices of anisotropic crystals. The results of the first measurements are in good agreement with those of other methods.     

细胞光阱刚度与折射率关系理论计算

为了探讨用光镊技术进行细胞折射率的测量方法,用几何光线理论对可作为米氏粒子模型的生物细胞(半径a=10μm,折射率n=1.35~1.70),在单光束梯度力光阱[激光波长λ=780 nm,功率P=6 mW,焦斑半径w(0)=0.6μm、0.8μm和1.0μm]中的轴向光阱刚度与细胞折射率关系进行了数值计算。结果表明,光阱刚度随折射率的变化关系与三次多项式曲线拟合得较好;用测量光阱刚度计算细胞的折射率时,需要用折射率已知的四种标准粒子对三次多项式曲线进行标定。     

高折射率玻璃微珠色散特性及影响

基于二次彩虹法用三种不同波长(632.8 nm、532 nm、404 nm)的激光测量了玻璃微珠的折射率,并由柯西色散公式拟合得到三种典型高折射率玻璃微珠1.90、1.93和2.2的色散方程,得出现行的玻璃微珠具有正常色散但色散较大的结论.通过分析在不同入射角情况下,色散对玻璃微珠回归反射性能的影响,得出折射率1.90和1.93的玻璃微珠具有优良的回归反射性能.此外,用光线追迹模拟了色散对回归反射后光能量在近轴区域分布的影响,折射率1.90和1.93的玻璃微珠回归反射光线的能量主要集中在5°范围内.     

Interferometric determination of refraction and dispersion of human blood-serum, saliva, sweat and urine

Multiple-beam interference fringes of equal chromatic order are produced in air and liquid sample interferometric gaps. The two gaps are of the same thickness and simultaneously enclosed in a wedge interferometer. A single shot interferogram containing fringes in the two gaps is sufficient to deduce the needed experimental data. Locations of the fringe maxima, in the two gaps, are introduced in a non-numerical procedure for determining the gap thickness and the liquid-phase refractive indices across the visible spectrum. The method has been used for measuring the phase refractive indices of human blood-serum, saliva, sweat, urine and water liquids. A third-order polynomial dispersion relation is applied for fitting the measured phase indices. Group refractive indices have been derived and fitted to the same dispersion formula.