Determination of Proteins Based on Their Resonance Light Scattering Enhancement Effect on Manganese-Tetrasulfonatophthalocyanine

This is the first report on the determination of proteins with manganese-tetrasulfonatophthalocyanine (MnTSPc) by resonance light scattering (RLS). At pH 3.6, the interactions of MnTSPc with the enhanced RLS signals at 344nm, proteins, including bovine serum albumin (BSA), human serum albumin (HSA), human -IgG, and ovalbumin, can be determined with a limit of detection below 20ngmL^–1. The method has been applied to the analysis of total protein in human serum samples collected from a hospital, and the results were in good agreement with those reported by the hospital.     

Rapid and sensitive determination of proteins by enhanced resonance light scattering spectroscopy of sodium lauroyl glutamate

A rapid and sensitive method for the determination of proteins is proposed based on the measurements of the enhanced resonance light scattering (RLS) spectroscopy of sodium lauroyl glutamate (SLG). Under the optimum conditions, the interaction between SLG and proteins occurred rapidly, resulting in greatly enhanced RLS intensity with the maximum peak located at 394 nm. It was found that the enhanced RLS intensities were in proportion to the concentrations of proteins in the range of 0.01-3.1 μg ml⁻¹ depending on the kind of proteins. The detection limits were below 6 ng ml⁻¹. Compared with some other methods for the determination of proteins, this method shows high sensitivity, low detection limit and simplicity. This is an inexpensive, simple and fast one-step procedure which requires only measuring the RLS intensities. Human serum samples were determined with satisfactory results.     

Resonance Double Light Scattering Method for the Determination of Nucleic Acids with Cetylpyridine Bromide

Cetylpyridine bromide (CPB) was used as a novel probe to determine nucleic acids by the resonance double light scattering technique in this paper. Under the optimum conditions, different nucleic acids have different binding properties with CPB. The sensitivity of this method decreases in the following order: ctDNA>yRNA>fsDNA. The detection limits are 8.9, 12.7 and 18.7ngmL^–1, respectively. Synthetic samples were analyzed satisfactorily.     

Bi-BSA-钙试剂体系共振光散射光谱法测定铋的研究

基于Bi(Ⅲ)可以对BSA-钙试剂体系的共振光散射产生一定的增强作用,从而建立了测定铋的灵敏的RLS新方法。该方法的线性范围为0.0~12.0μg/mL,检出限为4.5μg/L,对1.0μg/mL的铋标准溶液进行连续9次平等测定,相对标准偏差为1.4%。该方法用于胃药中铋量的测定。     

Study of the interaction of Azur B with DNA and the determination of DNA based on resonance light scattering measurements

Based on the measurements of molecular absorption and resonance light scattering (RLS), the aggregation of Azur B (AB) was in a medium of pH ranging from 1.98 to 2.56 and ionic strength <0.12 M. The presence of double stranded DNA prompts the aggregation, resulting in enhanced RLS signals. Linear relationships were achieved between the enhanced RLS intensity at 359.7 nm and DNA concentration in the range of 0-4.5 μg ml⁻¹ for both calf thymus DNA (ctDNA) and fish sperm DNA (fsDNA) if 3.0×10⁻⁵ M AB was employed. The 3σ limits of detection were 9.3 and 8.9 ng ml⁻¹ for ctDNA and fsDNA, respectively. Five synthetic samples were analysed satisfactorily.     

Ferric nanoparticle-based resonance light scattering determination of DNA at nanogram levels

A novel assay of DNA has been proposed by using ferric nanoparticles as probes coupled with resonance light scattering (RLS) detection. At pH 7.40, the RLS intensity of ferric nanoparticles can be greatly enhanced by the aggregation of positively charged ferric nanoparticles through electrostatic interaction with negatively charged DNA. The enhanced intensity of RLS at 452 nm is proportional to the concentration of DNA in the range of 0.01-0.8 μg ml⁻¹ for calf thymus and salmon sperm DNA and in the range of 0.005-0.3 μg ml⁻¹ for E. coli K12 genomic DNA. Detection limits are 3.6 ng ml⁻¹ for calf thymus DNA, 4.4 ng ml⁻¹ for salmon sperm DNA, and 1.9 ng ml⁻¹ for E. coli K12 genomic DNA, respectively. Compared with the chromophores previously used in RLS assay, the ferric nanoparticles have offered several advantages in easy preparation, good photostability and high sensitivity without being modified or functionalized.     

苋菜红—蛋白质体系的共振光散射光谱研究及其分析应用

研究了染色剂苋菜红与蛋白质的结合反应,在ＰＨ３．８７的Ｃｌａｒｋ－Ｌｕｂｓ缓冲介质中,苋菜红与蛋白质通过分子间作用力形成复合物。使最大波长约为３６４ｎｍ的共振光散射光谱得到加强。以苋菜红为标记物根据其共振光散射的增强程度。可用于蛋白质的定量测定,其线性响应范围为０－５．０ｍｇ／Ｌ。方法的稳定性及选择性良好,用于人血清试样中总蛋白的测定,结果与经典的考马斯亮兰法一致。     

Interactions of norfloxacin with DNA and determination of DNA at nanogram levels based on the measurement of enhanced resonance light scattering

A novel assay of DNA at nanogram levels is presented that is based on the measurement of resonance light scattering (RLS) signals in the presence of norfloxacin. The characteristics of RLS spectra, the effective factors and the optimum conditions of the reaction have been investigated. In Britton-Robinson buffer (pH 5.87), norfloxacin has a maximum peak at 405.5 nm and the RLS intensity is greatly enhanced by trace amounts of DNA due to the interaction between norfloxacin and DNA. Mechanistic studies show that the binding of norfloxacin to DNA forms large particles, which result in the significant enhancement of RLS intensity. The enhanced intensity of RLS is proportional to the concentration of DNA in the range from 0.01–2.0 μg mL⁻¹ for yeast DNA, and from 0.02 to 2.3 μg mL⁻¹ for calf thymus DNA. The determination limit (3σ) is 0.7 ng mL⁻¹ for yeast DNA and 1.2 ng mL⁻¹ for calf thymus DNA, respectively. Synthetic samples were determined satisfactorily.     

阿特拉津与DNA作用共振光散射光谱的研究及其应用

首次报道了阿特拉津与ctDNA作用的共振光散射光谱 (RLS)特征和利用小分子农药阿特拉津作为探针测定痕量脱氧核糖核酸的方法。在 pH=1.41的酸度条件下 ,阿特拉津 -ctDNA在319.8nm处有一增强的共振光散射光谱峰 ,且增强的共振光散射强度与ctDNA的浓度成线性关系。在实验确定的优化条件下 ,方法的线性范围为0.05～34μg·mL -1 ,检出限为11.9ng·mL -1(3δ) ,该方法成功地用于人工混合样品中ctDNA的测定     

用铬黑T作为共振光散射探针测定蛋白质

研究了金属指示剂铬黑T（EBT）作为共振光散射探针测定蛋白质的分析方法。实验表明，在pH=4.10的Britton-Robinson缓冲溶液条件下，铬黑T只有极弱的光散射，它与蛋白质结合后有强烈的共振光散射作用。在λ=375nm处，光散射强度最大，光散射强度与蛋白质的浓度成正比。据此建立了一种测定蛋白质的新方法。该方法简便、快速、灵敏度高，对HSA的检出限达到39μg/L;线性范围为0－15mg/L，用于人体血清样品的分析并用考马斯亮蓝法比较，取得了令人满意的结果。同时亦研究了牛血清白蛋白（BSA）、λ球蛋白、鸡蛋白蛋白、溶菌酶与染料EBT之间的作用。比较了2种不同类型的荧光仪器绘制的共振光散射光谱，并探讨了共振光散射的机理。     

Application of L-Cysteine-Capped ZnS Nanoparticles in the Determination of Nucleic Acids Using the Resonance Light Scattering Method

Nanometer-sized L-cysteine-capped ZnS particles were synthesized by a colloidal aqueous method. The functionalized nanoparticles are water-soluble and suitable for biological applications. In Tris-HCl buffer solution, nucleic acids combine with cysteine-capped nano-ZnS particles by intermolecular forces to form larger nanoparticles. There are two resonance light scattering peaks at 304.5nm and 373.8nm, respectively. The enhanced RLS is related to the concentration of nucleic acids in the range of 0.04 to 1.2µgmL^–1 for calf thymus DNA and 0.2 to 1.0µgmL^–1 for fish sperm DNA. The detection limits (3) are 19ngmL^–1 for calf thymus DNA and 23ngmL^–1 for fish sperm DNA, respectively. Four synthetic samples were analyzed satisfactorily.     

Determination of Nucleic Acids Based on their Resonance Light Scattering Enhancement Effect on Metalloporphyrin Derivatives

A new method for the determination of nucleic acids at nanogram per mL level is proposed based on the enhanced resonance light scattering (RLS) signal resulting from the interaction of metalloporphyrins with nucleic acids. Under optimum conditions, the weak RLS signal of metalloporphyrin is enhanced by nucleic acids, and the enhanced RLS intensity is proportional to the concentration of nucleic acids. The detection limits of calf thymus DNA were 3.5ngmL^–1, 2.9ngmL^–1 and 1.0ngmL^–1 for three metalloporphyrins, respectively. Synthetic samples were determined with satisfactory results.     

Resonance Light Scattering Spectra of Silver Thiocyanate System and its Application in Analysis

A novel determination method of Ag^＋ was established. In acetic acid-sodium acetate buffer （pH 5.0） medium, Ag^＋ reacts with SCN^- to form AgSCN in the presence of TritonX-100,which results in an increase of resonance light scattering （RLS）and giving a new RLS spectrum.The maximum RLS peak was at 585 nm,The enhancement of resonance light scattering at 585 nm was proportional to the concentration of Ag^＋ ranging from 0.0045-4.00μg mL^-1 （r=9991）,and the detection limit was 1.37 ng mL^-1 with the recovery of 97.70%- 104.80%。     

中性介质中中性红与双链DNA作用的光谱

通过分子吸收、荧光发射和共振光散射测定,表征了在水溶液介质中中性红(NR)与双 螺旋DNA的作用.在pH 7.63和离子强度低于0.01的水溶液介质中,随着NR与DNA的摩尔比(R)变 化,存在有两种结合方式.第一种结合方式发生在R > 2.22,此时获得共振光散射光谱增强信 号,表明NR在DNA分子表面发生聚集,集聚特性可使用RLS测定数据进行Scatchard分析;第二种 结合方式发生在R < 2.22,此时NR内嵌到DNA分子的双链碱基对之间,具有特征波长红移和分 子吸收增色效应,发生了从DNA到NR的分子能量转移,能观察到荧光增强.     

共振散射法测定中草药中的微量铬

研究了在H2SO4介质中,Cr(Ⅵ)与碘化物和淀粉形成离子缔合物的共振光散射增强现象,拟定了一种新的测定Cr(Ⅵ)的共振光散射方法,通过实验确定了溶液中Cr(Ⅵ)浓度与散射光强度之间的关系,在λex＝λem＝290 nm处,共振光散射最强,且共振光散射强度与Cr(Ⅵ)浓度呈线性关系,该方法简便快速,线性范围为34～400 μg/L,检出限为6.7 μg/L,可用于中草药样品中铬的测定.     

丽春红G 用于人血清样品中总蛋白的共振光散射测定

在酸性介质中 ,丽春红G (PonceauG ,PG)与牛血清白蛋白 (BSA)、人血清白蛋白 (HSA)、溶菌酶 (Lys)及γ 球蛋白 (γ IgG)等蛋白质作用产生共振光散射 (RLS)增强信号 ,最大散射峰位于 2 88nm处。在最佳酸度和离子强度下 ,增强RLS强度在 2 88nm处与蛋白质的浓度呈线性关系 ,用于测定BSA、HSA、Lys、γ IgG的检出限均在 2 5 μg L以下。本方法成功地应用于合成样和人血清样品的测定 ,测量结果与考马斯亮蓝法一致     

木质素桃红与蛋白质作用的共振光散射研究

研究了木质素桃红与蛋白质结合的共振光散射光谱. 实验结果表明, 在pH 2.56的酸性介质中, 木质素桃红与蛋白质发生静电作用产生以282.0、 346.0、 420.0 nm以及570.0 nm为特征峰的共振光散射（RLS）增强光谱. 在570.0 nm波长光激发下, 蛋白质的质量浓度与增强共振光散射强度ΔIRLS呈线性关系, 对BSA和HSA的检出限分别为39.0和22.3 ng/mL. 方法已用于尿样中总蛋白质分析.     

Determination of gamma-globulin at nanogram levels by its enhancement effect on the resonance light scattering of functionalized HgS nanoparticles

A novel assay of γ-globulin (γ-IgG) with a sensitivity at the nanogram level is proposed based on the measurement of enhanced resonance light-scattering (RLS) signals resulting from the interaction of functionalized nano-HgS with γ-globulin. At pH 5.03, the RLS signals of functionalized nano-HgS were greatly enhanced by γ-globulin in the region of 200-700 nm characterized by the peak around 362 nm. Linear relationship can be established between the enhanced RLS intensity and γ-globulin concentration in the range of 10-140 ng ml⁻¹. The limit of detection is 2.71 ng ml⁻¹. Based on this, a new direct quantitative determination method for γ-globulin in blood serum samples without separation of human serum albumin (HSA) is established. The contents of γ-IgG in blood serum samples were determined with recovery of 95.7-102.5% and R.S.D. of 1.6-2.4%. This method is proved to be very sensitive, rapid, simple and tolerance of most interfering substances.     

Resonance Light Scattering Method for Microdetermination of Proteins Based on Aggregation of Au Nanoparticles

By using a resonance light scattering (RLS) technique, a highly sensitive method for protein determination based on the aggregation of Au nanoparticles on protein template is described. For the Au nanoparticles of 15 nm, the detection limit of bovine serum albumin was 5.0 ng/mL and the linear range was 10–300 ng/mL. The experimental results indicated that various metal ions do not interfere with this assay. The proposed RLS assay exhibited lower variation in response signals for the same weight of different proteins and showed satisfactory results when it was used for determination of proteins in human serum.     

Determination of deoxyribonucleic acids by a resonance light scattering technique and its application

For the first time, acetamiprid has been used to determine nucleic acid (DNA) using the resonance light scattering (RLS). The RLS of acetamiprid was greatly enhanced by DNA in the range of pH 1.6-1.8. A RLS peak at 313 nm was found, and the enhanced intensity of RLS at this wavelength was proportional to the concentration of DNA. The linear range of the calibration curve was 0-11.0 microg ml(-1) with the detection limit of 20 ng ml(-1). The nucleic acids in synthetic sample and in rice seedling extraction were determined satisfactorily. The interaction mechanism of acetamiprid and DNA is discussed. Mechanism studies show that the enhanced RLS is due to the aggregation of acetamiprid in the presence of DNA.