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A conjugated polyelectrolyte poly[(2-methoxy-5-propyloxy sulfonate)-phenyl-ene vinylene] (MPS-PPV) drives the assembly of virus capsid proteins to form single virus-like particles (VLPs) and aggregates with more than two VLPs, with a relative selection of high molecular weight polymer in the latter. 相似文献
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Brasch M de la Escosura A Ma Y Uetrecht C Heck AJ Torres T Cornelissen JJ 《Journal of the American Chemical Society》2011,133(18):6878-6881
We report herein the encapsulation of a water-soluble phthalocyanine (Pc) into virus-like particles (VLPs) of two different sizes, depending on the conditions. At neutral pH, the cooperative encapsulation/templated assembly of the particles induces the formation of Pc stacks instead of Pc dimers, due to an increased confinement concentration. The Pc-containing VLPs may potentially be used as photosensitizer/vehicle systems for biomedical applications such as photodynamic therapy. 相似文献
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ZHAO Dong-hai ZHANG Xi-zhen YU Xiang-hui KONG Wei . College of Life Science Jilin University Changchun P. R. China . Jilin Medical College Jinlin 《高等学校化学研究》2008,24(5):579-583
The virus-like particle(VLPs) vaccine is an ideal HIV-1 vaccine, which can simultaneously induce a neutralizing antibody reaction and cell-mediated immunity effectively. In this study, two kinds of plasmids have been used, one can express the HIV-1 main structure proteins, Gagpol and Env, and the other contains an antibiotic gene. The two kinds of plasmids have been cotransfected into 293 cells. A stable cell line that can express Gagpol and Env proteins efficiently and lastingly has been screened. It has been confirmed that Gagpol and Env proteins in the cell culture supernatant can be self-assembled into virus-like particles. The authors have detected the secretion of VLPs in the cell medium, defined the peak of the secretion, and followed and monitored the stability of expression. 相似文献
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Rolle Rahikainen Pramila Rijal Tiong Kit Tan Hung‐Jen Wu Anne‐Marie C. Andersson Jordan R. Barrett Thomas A. Bowden Simon J. Draper Alain R. Townsend Mark Howarth 《Angewandte Chemie (International ed. in English)》2021,60(1):321-330
Matching of symmetry at interfaces is a fundamental obstacle in molecular assembly. Virus‐like particles (VLPs) are important vaccine platforms against pathogenic threats, including Covid‐19. However, symmetry mismatch can prohibit vaccine nanoassembly. We established an approach for coupling VLPs to diverse antigen symmetries. SpyCatcher003 enabled efficient VLP conjugation and extreme thermal resilience. Many people had pre‐existing antibodies to SpyTag:SpyCatcher but less to the 003 variants. We coupled the computer‐designed VLP not only to monomers (SARS‐CoV‐2) but also to cyclic dimers (Newcastle disease, Lyme disease), trimers (influenza hemagglutinins), and tetramers (influenza neuraminidases). Even an antigen with dihedral symmetry could be displayed. For the global challenge of influenza, SpyTag‐mediated display of trimer and tetramer antigens strongly induced neutralizing antibodies. SpyCatcher003 conjugation enables nanodisplay of diverse symmetries towards generation of potent vaccines. 相似文献
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Viruses belong to a fascinating class of natural supramolecular structures, composed of multiple copies of coat proteins (CPs) that assemble into different shapes with a variety of sizes from tens to hundreds of nanometres. Because of their advantages including simple/economic production, well-defined structural features, unique shapes and sizes, genetic programmability and robust chemistries, recently viruses and virus-like nanoparticles (VLPs) have been used widely in biomedical applications and materials synthesis. In this critical review, we highlight recent advances in the use of virus coat proteins (VCPs) and viral nanoparticles (VNPs) as building blocks in self-assembly studies and materials development. We first discuss the self-assembly of VCPs into VLPs, which can efficiently incorporate a variety of different materials as cores inside the viral protein shells. Then, the self-assembly of VNPs at surfaces or interfaces is summarized. Finally, we discuss the co-assembly of VNPs with different functional materials (178 references). 相似文献
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环硅氧烷在亲核或亲电催化剂、温度或辐射作用下,可开环聚合生成线型聚硅氧烷,聚合方法主要有本体聚合和乳液聚合.本体聚合可分为阴离子聚合和阳离子聚合,阴离子聚合就是在碱性催化剂(亲核试剂)作用下,使环硅氧烷开环聚合成线型聚硅氧烷的过程;阳离子聚合就是环硅氧烷在酸性催化剂(亲电试剂)作用下的开环聚合反应.乳液聚合则是单体和水(或其它分散介质)并用乳化剂配成乳液状态进行聚合,按所采用的乳化剂种类不同,主要有阴离子型和阳离子型两种类型.本文总结了近几年国内外环硅氧烷本体聚合和乳液聚合的开环聚合机理及动力学研究情况,并对今后此方面的研究进行了展望. 相似文献
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含氨基、羟基丙烯酸乳液聚合的稳定性 总被引:12,自引:1,他引:11
采用间歇及半连续乳液聚合方式,以过硫酸铵/亚硫酸钠为引发体系,合成了甲基丙烯酸甲酯/丙烯酸丁酯/甲基丙烯酸羟乙酯/甲基丙烯酸二氨基乙酯四元共聚物胶乳.系统研究了乳化剂种类和浓度、聚合温度、乳化单体进料方式及进料速率对聚合过程稳定性的影响.聚合温度降低,乳化单体进料速度减慢有利于聚合过程的稳定,采用种子半连续聚合方式比间歇聚合过程更稳定,乳化剂浓度的增加有利于聚合稳定性的提高和乳胶粒子的均匀化. 相似文献
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《中国化学快报》2019,30(12):1996-2002
This short review is dedicated to celebrate Prof.Shoukuan Fu's 80 th birthday by discussing several of my accomplished projects over the past twenty years,which all applied radical polymerization in aqueous dispersed media for producing polymers with branched structures.These projects include the use of microemulsion polymerization for syntheses of fluorescent nanoparticles,hairy nanoparticles and hyperbranched polymers;the use of miniemulsion polymerization for synthesis of star polymers and light-emitting nanoparticles;the use of seeded emulsion polymerization for synthesis of hairy nanoparticles and hyperstar polymers;and the use of precipitation polymerization for synthesis of hollow polymer nanocapsules.Discussion of these projects demonstrates intriguing features of polymerization in biphasic dispersed media via either conventional radical polymerization or controlled radical polymerization to effectively regulate the branched structure of functional polymers. 相似文献
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Haifeng Gao 《中国化学快报》1990,30(12):1996-2002
Several projects were discussed to demonstrate the intriguing power of radical polymerization in aqueous dispersed media to regulate the branched structures in functional polymeric nanomaterials. 相似文献
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Dr. Kun Zhou Yihao Zhou Dr. Hongchao Yang Prof. Dr. Huile Jin Prof. Dr. Yonggang Ke Prof. Dr. Qiangbin Wang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(41):18406-18412
We present a strategy of interfacially bridging covalent network within tobacco mosaic virus (TMV) virus-like particles (VLPs). We arranged T103C cysteine to laterally conjugate adjacent subunits. In the axis direction, we set A74C mutation and systematically investigated candidate from E50C to P54C as the other thiol function site, for forming longitudinal disulfide bond chains. Significantly, the T103C-TMV-E50C-A74C shows the highest robustness in assembly capability and structural stability with the largest length, for TMV VLP to date. The fibers with lengths from several to a dozen of micrometers even survive under pH 13. The robust nature of this TMV VLP allows for reducer-free synthesis of excellent electrocatalysts for application in harshly alkaline hydrogen evolution. 相似文献
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Kun Zhou Yihao Zhou Hongchao Yang Huile Jin Yonggang Ke Qiangbin Wang 《Angewandte Chemie (International ed. in English)》2020,59(41):18249-18255
We present a strategy of interfacially bridging covalent network within tobacco mosaic virus (TMV) virus‐like particles (VLPs). We arranged T103C cysteine to laterally conjugate adjacent subunits. In the axis direction, we set A74C mutation and systematically investigated candidate from E50C to P54C as the other thiol function site, for forming longitudinal disulfide bond chains. Significantly, the T103C‐TMV‐E50C‐A74C shows the highest robustness in assembly capability and structural stability with the largest length, for TMV VLP to date. The fibers with lengths from several to a dozen of micrometers even survive under pH 13. The robust nature of this TMV VLP allows for reducer‐free synthesis of excellent electrocatalysts for application in harshly alkaline hydrogen evolution. 相似文献
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预聚合条件对高效催化剂催化乙烯气相聚合的影响 总被引:1,自引:0,他引:1
采用经预聚合处理的MgCl2负载的钛系催化剂进行乙烯气相聚合和淤浆聚合比较.乙烯淤浆聚合其表观动力学呈现为加速型或渐升衰减混合型,而气相聚合则为衰减型.在聚合前期阶段,气相聚合速度明显高于淤浆聚合速度.这样的差别是由于淤浆聚合中明显存在着单体在颗粒中扩散受阻的影响,气相聚合中则存在过热现象.测定了两种聚合方式中催化剂的钛价态,结果表明气相聚合条件下钛的价态分布比淤浆聚合更大程度倾向于低价态.催化剂预聚合温度和预聚合产率对气相聚合活性及表观动力学行为的影响表明预聚合工艺是气相聚合反应平稳控制有效技术. 相似文献
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本实验开展了醋酸乙烯酯的可逆加成-断裂链转移自由基聚合(RAFT),分别运用偶氮二异丁腈(AIBN)引发和可见光引发两种方式进行了聚合反应,运用核磁共振和凝胶色谱等多种手段对所得聚合物结构进行了表征与分析。通过比较AIBN引发与光引发所获得聚合物端基结构的异同,加深了学生对RAFT聚合方法原理的理解。同时,运用该方法实现了聚合物两端端基结构的高度功能化,深入体会聚合物合成设计概念。本综合实验教学不仅通过对比法加深了学生对实验原理和专业知识的理解,提升了学生创新研究能力,而且训练了学生的实验操作技能、大型仪器使用能力和结果分析能力,提升了综合素质。 相似文献