Chemical investigation of biomass of a culture of ginseng cells III. Polysaccharides of a callus culture of ginseng

A water-soluble polysaccharide fraction has been isolated from a callus culture of ginseng with a yield of 16–20%. It has been shown that it included starch a and acidic polysaccharides — arabinogalactans and a xyloglucan.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Branch, USSR Academy of Sciences, Vladivostok, All-Union Scientific Research Institute of Biochemical Machinery Design, USSR Ministry of the Medical Industry, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 771–772, November–December, 1989.     

Chemical investigation of the biomass of a ginseng culture

Callus cultures and radical cultures from the roots of plantation ginseng plants of the Maritime Territory population have been obtained. It has been established that, with respect to the level of ginsenosides that they contain, the new cultures are close to those parts of the root from which they were obtained; the spectrum of the ginsenosides in the culture is somewhat narrower than in the root. Some of the lines obtained are superior to the domestic cultures of ginseng described previously with respect both to the amount and to the variety of ginsenosides. All-Union Scientific-Research Institute for the Design of Biochemical Machinery, Ministry of the Medical Industry, Moscow. Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 808–813, November–December, 1991.     

Chemical investigation of the biomass of a ginseng culture

Callus cultures and radical cultures from the roots of plantation ginseng plants of the Maritime Territory population have been obtained. It has been established that, with respect to the level of ginsenosides that they contain, the new cultures are close to those parts of the root from which they were obtained; the spectrum of the ginsenosides in the culture is somewhat narrower than in the root. Some of the lines obtained are superior to the domestic cultures of ginseng described previously with respect both to the amount and to the variety of ginsenosides.All-Union Scientific-Research Institute for the Design of Biochemical Machinery, Ministry of the Medical Industry, Moscow. Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 808–813, November–December, 1991.     

Polysaccharides of plant tissue cultures. III. Enzymatic hydrolysis of industrial wastes of the biomass of a ginseng tissue culture

Questions of the enzymatic hydrolysis of industrial wastes of the biomass of a ginseng tissue culture by Pektofoetidin P10× (I), Tsellyulaza P10× (II), and Tsellokoningin P10× (III) are discussed. The optimum conditions permitting the hydrolysis of the wastes by the following respective percentages have been selected: (I) 62; (II) 43; (III) 54. Subsequent treatment of the wastes with mineral acids raises the total degree of hydrolysis to 84–88%. The monocarbohydrate compositions of the hydrolysates have been studied by chromatographic methods. Student I. B. Smirnova took part in the work.     

Polysaccharides of plant tissue cultures. III. Enzymatic hydrolysis of industrial wastes of the biomass of a ginseng tissue culture

Questions of the enzymatic hydrolysis of industrial wastes of the biomass of a ginseng tissue culture by Pektofoetidin P10× (I), Tsellyulaza P10× (II), and Tsellokoningin P10× (III) are discussed. The optimum conditions permitting the hydrolysis of the wastes by the following respective percentages have been selected: (I) 62; (II) 43; (III) 54. Subsequent treatment of the wastes with mineral acids raises the total degree of hydrolysis to 84–88%. The monocarbohydrate compositions of the hydrolysates have been studied by chromatographic methods.Student I. B. Smirnova took part in the work.M. V. Frunze Simferopol' State University. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 492–499, July–August, 1988.     

Chemical investigation of the biomass of a culture of ginseng cells. IV. Quantitative analysis of the ginsenosides of the total glycosidic fraction by the HPLC method

The total glycosidic fractions of four samples of ginseng cell cultures have been analyzed by reversed phase high-performance liquid chromatography, and it has been shown that only the BIO-2 samples contained ginsenosides — protopanaxadiol derivatives. The concentrations of these ginsenosides in the biomass of the cell culture was considerably lower than in the natural root. Samples of the biomass of a culture of cells of the IFRZh-1 strains contained no ginsenosides.Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. All-Union Scientific-Research Institute of Biochemical Machinery Design, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 361–363, May–June, 1990.     

Chemical investigation of the biomass of a culture of ginseng cells. IV. Quantitative analysis of the ginsenosides of the total glycosidic fraction by the HPLC method

The total glycosidic fractions of four samples of ginseng cell cultures have been analyzed by reversed phase high-performance liquid chromatography, and it has been shown that only the BIO-2 samples contained ginsenosides — protopanaxadiol derivatives. The concentrations of these ginsenosides in the biomass of the cell culture was considerably lower than in the natural root. Samples of the biomass of a culture of cells of the IFRZh-1 strains contained no ginsenosides. Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. All-Union Scientific-Research Institute of Biochemical Machinery Design, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 361–363, May–June, 1990.     

Phenylpropanoids of a callus culture of Rhodiola rosea

Using 11 phenylpropanoids isolated from the biomass of a callus culture of roseroot stonecrop, the component compositions in the biomasses of callus and suspension cultures have been studied and their triandrin contents have been determined by the HPLC method. All-Union Scientific-Research Institute of Medicinal Plants, Scientific Production Combine, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 481–490, July–August, 1991.     

Phenylpropanoids of a callus culture of Rhodiola rosea

Using 11 phenylpropanoids isolated from the biomass of a callus culture of roseroot stonecrop, the component compositions in the biomasses of callus and suspension cultures have been studied and their triandrin contents have been determined by the HPLC method.All-Union Scientific-Research Institute of Medicinal Plants, Scientific Production Combine, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 481–490, July–August, 1991.