共查询到20条相似文献,搜索用时 0 毫秒
1.
A simple benzoyl hydrazine derivative P was successfully synthesized and characterized as Mg2+-selective fluorescent probe. The binding of P with Mg2+ caused an obvious fluorescence enhancement at 482 nm. The fluorescent, UV-vis spectra, 1H-NMR, and IR spectra confirmed the formation of P-Mg2+ complex, and the formation of a 1:1 stoichiometry complex was proved by Job’s plot and mass spectrometry. The recognition mechanism of P to Mg2+ was owing to the photoinduced electron transfer effect (PET). The fluorescent response was linear in the range of 0.9–4.0 µM with the detection limit of 0.3 µM Mg2+ in water–ethanol solution (1:9, v:v, pH10.0, 20 mM HEPES). In addition, the results of cell imaging of Mg2+ in Hl-7701 cells was satisfying. 相似文献
2.
建立了一种以牛血清白蛋白功能化的金纳米棒(BSA-Cys-GNRs)为荧光探针检测Hg2+的新方法。以半胱氨酸作为连接臂成功将牛血清白蛋白修饰在金纳米棒表面,通过紫外可见吸收光谱、荧光光谱、红外光谱和荧光倒置显微镜等多种分析方法对材料进行表征。研究发现,在295nm波长光激发下,BSA-Cys-GNRs探针在338nm显示强荧光,而Hg2+能够有效地猝灭BSA-Cys-GNRs的荧光。对一系列影响猝灭效果的因素进行考察,得出pH 4.0、孵育时间5.0min为最佳检测条件。Mn2+、K+、Ni2+、Na+、Cr3+、Cd2+、Mg2+、Cu2+、Ca2+、Al3+和Zn2+对BSA-Cys-GNRs的荧光信号没有明显的影响。当Hg2+的浓度为0.04444~8.888μmol·L-1时,荧光猝灭效率与Hg2+的浓度之间存在良好的线性关系,检测限为8.08nmol·L-1。将该方法用于环境水样中Hg2+的检测,回收率为98.9%~105.0%。 相似文献
3.
设计合成了一种可逆的Hg2+荧光增强型分子探针3’,6’-双(二乙氨基)-2-((2-羟基-4-甲氧基苯亚甲基)氨基)螺[异吲哚-1,9’-氧杂蒽]-3-硫酮(RBS),并利用红外光谱、元素分析、核磁等方法对探针结构进行表征。探针本身荧光很弱,与Hg2+结合后荧光显著增强,由此,建立了Hg2+测定的新方法。该方法对Hg2+测定的线性范围为5.00×10-9~1.10×10-6mol/L,检出限为2.82×10-9mol/L,具有操作简单、灵敏度高、选择性好、线性范围宽等特点。将该方法用于河水及土壤样品中Hg2+的加标回收实验,结果满意。 相似文献
4.
《Analytical letters》2012,45(17):2751-2761
Quinoline-appended rhodamine B thiohydrazide based fluorescent probe was designed and applied in fluorescent detections of mercury ions in both aqueous solution and living cells. The signal change of the probe is based on a specific metal ion induced reversible ring-opening mechanism of a rhodamine B thiohydrazide. The probe exhibits a dynamic response concentration range for Hg2+ from 1.0 × 10?8 to 1.0 × 10?5 M with a detection limit of 8.5 × 10?9 M. The fluorescent probe is pH independent in medium condition and exhibits high selectivity over other common metal ions. 相似文献
5.
6.
Bi-Jie Bie Xiao-Rui Zhao Jia-Rui Yan Xi-Jun Ke Fan Liu Guo-Ping Yan 《Molecules (Basel, Switzerland)》2022,27(19)
Fluorescent imaging has been expanded, as a non-invasive diagnostic modality for cancers, in recent years. Fluorescent probes in the near-infrared window can provide high sensitivity, resolution, and signal-to-noise ratio, without the use of ionizing radiation. Some fluorescent compounds with low molecular weight, such as rhodamine B (RhB) and indocyanine green (ICG), have been used in fluorescent imaging to improve imaging contrast and sensitivity; however, since these probes are excreted from the body quickly, they possess significant restrictions for imaging. To find a potential solution to this, this work investigated the synthesis and properties of novel macromolecular fluorescent compounds. Herein, water-soluble dextran fluorescent compounds (SD-Dextran-RhB) were prepared by the attachment of RhB and sulfadiazine (SD) derivatives to dextran carrier. These fluorescent compounds were then characterized through IR, 1H NMR, 13C NMR, UV, GPC, and other methods. Assays of their cellular uptake and cell cytotoxicity and fluorescent imaging were also performed. Through this study, it was found that SD-Dextran-RhB is sensitive to acidic conditions and possesses low cell cytotoxicities compared to normal 293 cells and HepG2 and HeLa tumor cells. Moreover, SD-Dextran-RhB demonstrated good fluorescent imaging in HepG2 and HeLa cells. Therefore, SD-Dextran-RhB is suitable to be potentially applied as a probe in the fluorescent imaging of tumors. 相似文献
7.
8.
9.
10.
A simple fluorescent probe, which contains rhodamine and aminoquinoline moieties, was designed and prepared for selective detection of Hg2+ in acetonitrile. RbQ exhibited high selectivity and sensitivity toward Hg2+ over other common metal ions. The recognition of RbQ toward Hg2+ can be detected by fluorescence spectra, absorption spectra, and even by naked eyes. The binding ratio of the RbQ–Hg2+ complex was found to be 1:1 according to Job plot experiment, and the limit of detection was 1.05×10−7 M. Moreover, the prepared complex RbQ–Zn2+ (RbQZ) could detect Hg2+ in a ratiometric way and showed lower limit of detection (2.95×10−8 M) than RbQ in the same condition. Finally, we also demonstrated that the aminoquinoline–zinc complex could be served as a new and effective FRET donor for rhodamine derivatives. 相似文献
11.
基于硼二吡咯亚甲基类(BODIPY)衍生物的合成,光谱及汞离子识别(英文) 总被引:1,自引:0,他引:1
The synthesis and sensing properties of a new BODIPY derivative 1 are outlined. 1 shows fluorescence “turn-on” and colorimetric responses with high selectivity toward Hg2+ over the other metal cations. Coordination of Hg2+ influences the electronic properties of the receptor at meso-position and alter the efficiency of non-radiative decay, hence increase the fluorescence intensity and red shift the absorption spectrum. 相似文献
12.
13.
一种新型Zn2+近红外荧光探针的研究 总被引:2,自引:0,他引:2
Zn2+是多种酶和转录因子的必要成分, 在生物体内起着重要的作用, 检测Zn2+具有十分重要的生物学意义. 我们合成了一种新型近红外荧光探针TAEA-IR-780, 该探针通过配位作用与Zn2+结合后, 荧光显著增强. 通过质谱和氢谱对该探针进行了表征, 并探讨了pH值、其它金属离子对Zn2+检测的影响. 该探针的激发波长为683 nm, 发射波长为750 nm, 可避免对生物活体的损伤, 同时生物体内常见的金属离子对其干扰较小. 该探针对Zn2+的检测下限达1.0× 10-9 mol•L-1, 具有较高的检测灵敏度. 相似文献
14.
该文设计并合成了一种基于菲并咪唑的新型次氯酸荧光探针,该探针由商用试剂9,10-菲醌和4-(甲基巯基)苯甲醛通过一步反应生成,只需简单后处理(过滤和水洗)即可得到大量纯品(产率达85%)。由于硫的重原子效应,探针荧光较弱。但在次氯酸存在下,探针分子内的硫原子被氧化成亚砜,重原子效应减弱,同时分子内电荷转移效应(ICT)加强,导致荧光增强。借助高分辨质谱,证明了探针与次氯酸盐的反应机理。在PBS缓冲溶液中(DMF∶H2O=2∶8,体积比,pH 7.4),探针MPI可定量检测0~100μmol/L范围内的HClO,检出限为0.26μmol/L。该探针对次氯酸具有较高的灵敏性和选择性。更重要的是,MPI具有良好生物相容性和细胞渗透性,可用于活细胞内次氯酸的可视化成像。探针MPI有望为机体内次氯酸的检测与追踪提供一种可靠、有效的方法。 相似文献
15.
16.
A new fluorescein-based chemodosimeter (II) for Hg2+ ion was designed and synthesized, and it displayed excellent selective and sensitive toward Hg2+ ion over other commonly metal ions in aqueous media. II was a colorless, non-fluorescent compound. Upon addition of Hg2+ to the solution of II, the thiosemicarbazide moiety of II would undergo an irreversible desulfurization reaction to form its corresponding oxadiazole (IV), a colorful and fluorescent product. During this process, the spirocyclic ring of II was opened, causing instantaneous development of visible color and strong fluorescence emission in the range of 500-600 nm. Based on the above mechanism, a fluorogenic Hg2+-selective chemodosimeter was developed. The fluorescence increase is linearly with Hg2+ concentration up to 1.0 μmol L−1 with a detection limit of 8.5 × 10−10 mol L−1 (3σ). Compared with the rhodamine-type chemodosimeter, II is more stable in aqueous media and exhibits higher sensitivity toward Hg2+. The findings suggest that II will serve as a practical chemodosimeter for rapid detection of Hg2+ concentrations in realistic media. 相似文献
17.
以罗丹明B与1,8-萘二甲酰亚胺反应合成了1个高选择性Hg2+比率荧光探针(RN). 在甲醇/乙腈/4-羟基哌嗪乙磺酸缓冲溶液(pH=7.2, 体积比8:1:1)中, RN对Hg2+具有比色和比率荧光双重响应. 加入Hg2+后, RN的紫外-可见光谱在约556 nm处产生强吸收, 溶液由浅绿色变为橙色, 其它金属离子对RN的紫外-可见光谱几乎无影响. 无Hg2+存在时, RN的荧光光谱在540 nm处出现萘二甲酰亚胺荧光团的特征峰; 加入Hg2+后, 540 nm处的发射带逐渐消失, 同时在580 nm附近产生强荧光, 荧光颜色从绿色变为橙色. 这归因于从萘酰亚胺到开环罗丹明B的荧光共振能量转移(FRET), 探针RN对Hg2+的比率荧光响应具有高选择性, 不受其它共存金属离子的干扰. 相似文献
18.
该文以2-(2′-羟基-3′-醛基-5′-甲基苯基)苯并噻唑和1,1-二甲基肼为原料,通过一步缩合,简单过滤处理得到一种基于苯并噻唑的新型ONOO-荧光探针BD。但由于N—N单键旋转产生非辐射能量损失,探针BD荧光较弱。在引入ONOO-后,探针BD上的腙水解成醛基,N—N单键脱落,荧光增强,同时可观察到明显的颜色变化。在PBS缓冲溶液(DMSO∶H2O=2∶8,体积比,pH 7.4)中,探针BD对ONOO-具有快速响应(25 s)、高灵敏度(7 nmol/L)和高选择性的特点,可在较宽pH值范围内工作。此外,探针BD还成功地用于肝癌细胞中ONOO-的荧光成像,因此,可作为揭示ONOO-在细胞中作用的一个很有潜力的分析工具。 相似文献
19.
A new fluorescent probe for the detection of Hg2+ was designed and synthesized via attaching N-methylisatin to rhodamine B hydrazide through an imine linkage. In an ethanol-Tris buffer medium, the addition of Hg2+ caused a strong orange fluorescence and a visual color change from colorless to pink. Other coexisting metal ions did not interfere with the detection of Hg2+. The research on the detection of Hg2+ in natural water suggested the possibility of practical applications in environment monitoring. Based on ESI-MS analysis, the Hg2+-sensing mechanism was proposed. 相似文献
20.
设计合成了一种用于检测Hg2+的苯并噁唑类荧光探针4-(苯并[d]噁唑-2-基)-2-((二(2-(乙巯基)乙基)氨基)甲基)苯酚(ZY1), 用质谱、核磁氢谱和X射线单晶衍射等方法对其进行了结构表征, 并考察了其光谱性能. 研究结果表明, ZY1对Hg2+有较高的选择性和灵敏度, 它们之间的结合比为1:1, 共存离子及pH值(3.0~9.0)对其测定影响小. ZY1能够可视化检测Hg2+, 并在HeLa活细胞中实现了汞离子的成像, 这对检测生物体内的Hg2+将具有潜在的应用价值. 相似文献