微量元素与激素

微量元素可以在激素的分泌、活性以及与组织的结合等各个环节上影响激素。反之，激素也可以调控机体微量元素的代谢过程。     

微量元素与小儿厌食

人体必需微量元素的不足或缺乏,主要是缺乏时即会引起机体生物功能及生化代谢的紊乱或异常,尤其是小儿。小儿由于生长发育快,对各种必需微量元素需求量相对增加,因而特别容易引起必需微量元素和宏量元素的不足或缺乏。     

KIO4-偶氮氯膦mA-邻菲啰啉体系催化光度法测定痕量锰

1引言 现代医学研究表明,微量元素作为机体的营养物质与人体的正常代谢有密切关系.     

人体微量元素平衡与健康饮食

综述了人体微量元素平衡与健康饮食的关系。微量元素参与机体内的各种代谢,在维持人的生命活动中发挥着重要的作用。正确理解微量元素的生理功能,树立科学的平衡营养观,是引导人们自觉地实现多样化饮食、促进健康长寿的关键。     

360例鼻咽癌患者头发微量元素调查报告

为探讨鼻咽癌患者机体内微量元素的含量，作者对３６０例鼻咽癌患者在放疗前进行头发Ｚｎ、Ｆｅ、Ｃａ、Ｃｕ、Ｎｉ含量的测定，并对各年龄及性别间的微量元素进行对比．结果表明，头发Ｚｎ男性鼻咽癌患者明显高于女性（Ｐ＜０．０１），头发Ｆｅ老年组鼻咽癌患者明显高于青年组（Ｐ＜０．０５），头发钙青年组鼻咽癌患者明显高于老年组（Ｐ＜０．０５），而头发ＣＵ、Ｎｉ各年龄组及性别间均无明显差异。     

微量元素与遗传性代谢病

综合了有关研究，对微量元素与遗传性代谢病之间的关系，从发病原因和治疗机理方面进行了分析，并对微量元素和基因的调控进行了初探，认为从非遗传的调控机制入手，研究微量元素的代谢机理，可能在遗传性代谢病发生和治疗上具有目前尚无法预见的潜力。     

补中益气汤对肝硬化患者机体微量元素的影响

为探讨补中益气汤对肝硬化患者机体微量元素水平的影响,采用全自动生化分析仪检测了30例服用补中益气汤肝硬化患者及30例肝硬化对照组患者血清中镁、铁、铜、锌的水平。结果表明,服用补中益气汤患者血清镁、铁、锌水平均显著高于对照组患者(P0.05),服用补中益气汤患者血清铜水平均显著低于对照组患者(P0.05)。提示了补中益气汤能显著提高肝硬化患者机体微量元素水平,对改善患者肝脏功能具有一定的作用。     

白血病骨髓移植患者口腔黏膜病与微量元素锌的关系

为研究白血病骨髓移植患者全血微量元素锌与口腔黏膜病的关系，用原子吸收光谱法检测了正常对照组与白血病骨髓移患者预处理前及移植后骨髓空虚期全血微量元素锌的含量，并观察了白血病骨髓移植患者空黏膜病变情况。结果表明，正常对照组全血锌浓度与白血病骨髓移植患者预处理前全血锌浓度比较，差异无显著性（P＞0．05），而预处理前全血锌浓度与骨髓空虚期全血锌浓度有显著性差异（P＜0．01），白血病缓解后（预处理前）血锌接受正常水平，无口腔溃疡发生，而白血病骨髓移植患者处理后骨髓空虚期全血锌含量显著降低，并出现不同程度的口腔黏膜病变，说明预处理影响微量元素锌的代谢，微量元素锌的减少与白血病骨髓移植患者移植患者口腔黏膜病的发生有关。     

电感耦合等离子体质谱法测定血液中14种微量元素

<正>微量元素在人体中的含量及代谢与人体健康有着密切的关系。当人们体内进行各种生理活动或者参加外界运动时,微量元素将起到不同的功效,摄入的量过多、不足或不平衡都会不同程度地引起人体生理的异常或疾病的发生~([1-2])。在患者未出现临床症状之前,在血液、尿液、活体组织(如毛发、指甲等)中可发现一些微量元素及代谢衍生物的变化。目前一些医疗机构通过检测头发或者血液中的微量元素,来判断受检者体内微量元素含量是否正常。     

复方黄芩滴鼻液的制备与临床应用

复方黄芩滴鼻液对过敏性鼻炎有确切疗效，通过对２７例过敏性鼻炎患者观察，２０例有效，５例好转，且无毒副作用，药效高，安全可靠，又因含有人体必需的微量元素及维生素等，而增加了机体的免疫力，使治愈效果增加。     

ssDNA-QDs/GO multicolor fluorescence system for synchronous screening of hepatitis virus DNA

Viral hepatitis is a common infectious disease caused by five viruses (hepatitis virus A, B, C, D, and E). Given the diversity of hepatitis virus, rapid screening and accurate typing of viral hepatitis are the prerequisites for hepatitis therapy. Here, a multicolor fluorescence system was constructed by combining with the multi-color fluorescence properties of CdSe/ZnS quantum dots (QDs, emission wavelengths: 525 nm, 585 nm and 632 nm) and the broad-spectrum fluorescence quenching performance of GO. Taking advantage of the specific recognition of ssDNA modified CdSe/ZnS QDs to target hepatitis virus DNA, the constructed system could effectively distinguish hepatitis A virus DNA (HAV-DNA), hepatitis B virus DNA (HBV-DNA), and hepatitis C virus DNA (HCV-DNA) in a homogeneous solution. Based on the different adsorption property of GO for ssDNA and dsDNA, the fluorescence Forster resonance energy transfer (FRET) process between ssDNA modified QDs and GO could be regulated. The fluorescence signal of the constructed system presented a sensitive response to HAV-DNA, HBV-DNA, and HCV-DNA content in the range of 1.0–192 nM, 8.0–192 nM, and 1.0–128 nM, respectively. The limit of detection for HAV-DNA, HBV-DNA, and HCV-DNA is 0.46 nM, 1.53 nM, and 0.58 nM. The constructed system can be used to screen hepatitis virus DNA in real samples, which provides an alternative strategy for rapid screening and diagnosis of viral hepatitis.     

Detection of duck hepatitis B virus DNA fragments using on-column intercalating dye labeling with capillary electrophoresis-laser-induced fluorescence.

A rapid on-column DNA labeling technique is used to detect viral restriction DNA fragments by capillary electrophoresis-laser induced fluorescence detection. Intercalating dyes such as POPO3 or ethidium homodimer-2 are incorporated into the detection buffer. The cationic dyes migrate into the capillary during electrophoresis and bind to the oppositely migrating DNA fragments. A post-column sheath-flow fluorescence detector is used in the experiment. Excellent labeling efficiency is achieved at minimal background fluorescence by diluting the dyes to between 1 x 10(-7) M and 5 x 10(-7) M in a buffer with low ionic strength relative to the running buffer within the capillary. This dilute sheath-flow buffer allows stacking of dye molecules inside the capillary when an electric field is applied. Calibration curves using a series of DNA size markers (between 72 and 1353 base pairs) were linear over an order of magnitude in DNA concentration. Sensitivity also increased linearly with fragment length, and detection limits ranged from 4 x 10(-14) M to 5 x 10(-13) M for the size-standards. Analysis of cloned viral DNA using duck hepatitis B virus demonstrated a concentration detection limit of 3.9 x 10(-16) M. Last, the technique produced very high separation efficiency, 14 x 10(6) theoretical plates which is greater than 47 x 10(6) plates m-1, for the duck hepatitis B viral genome.     

硒卡拉胶囊治疗慢性乙型肝炎

用硒卡拉胶囊治疗了慢性乙型肝炎 49例。结果表明 ,硒卡拉胶囊对慢性乙肝病人乏力等症状有明显改善作用 ,其ALT和SB复常率分别为 95 9%和 96 8% ,优于对照 (P <0 0 5 ) ,说明补硒治疗慢性乙肝有一定的改善临床症状、降酶等作用。     

Development of a High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Newly Co-formulated Antiviral Drugs Sofosbuvir and Velpatasvir in Their Pure Forms and Tablet Dosage Form

JPC – Journal of Planar Chromatography – Modern TLC - The discovery of new direct-acting antiviral drugs gave rise to a leap forward in the treatment of hepatitis C viral infections....     

肝生素治疗40例重型乙型病毒性肝炎的疗效初探

应用肝生素治疗各种重型乙型病毒性肝炎,结果表明,４０例重型乙型病毒肝炎患者经肝生素治疗,治疗组死亡１５例,病死率３７．５％,对照组死亡２６例,病死率６５．０％,两组比较有显著差异。肝生素在重型肝炎的早中期治疗效果特别理想,可认为肝生素是目前治疗重型病毒性肝炎比较理想的护肝药物。     

Ribozyme diagnostics comes of age

Biosensing ribozymes could soon be used to diagnose viral infection. The Kossen group from Sirna Therapeutics have developed a sensitive, high-throughput means of screening for hepatitis C virus, using their target activated half-ribozyme technology, as reported in the June issue of Chemistry & Biology.     

Introduction of the gene amplification technique to decrease the risk of hepatitis C virus transmission by plasma products.

The viral safety of plasma-derived products with respect to hepatitis C virus (HCV) is assured by selection of donors, screening of individual donations for antibodies to HCV and the incorporation of effective viral inactivation-removal steps into manufacturing processes. As antibody screening of single donations is not sufficient to completely eliminate HCV RNA positive plasmas from plasma pools, testing for HCV RNA by gene amplification techniques may be necessary to identify positive donations. Using modern molecular biology techniques, we developed a specific, sensitive and reproducible method for routine PCR screening for HCV RNA in plasma pools.     

Significance of anti-HBc IgM in acute hepatitis and hepatitis B associated chronic liver disease

To determine the diagnostic value of anti-HBc IgM in acute viral hepatitis or chronic liver disease B, Anti-HBc IgM was measured by a RIA and an ELISA in 32 patients with acute hepatitis (4 with type A, 15 with type B and 13 with type non A non B), 18 patients with chronic hepatitis and 19 patients with liver cirrhosis. In acute hepatitis B, anti-HBc IgM (both RIA and ELISA) was positive in 14(93%) of 15 patients and its cut-off index value was very high. However, anti-HBc IgM was always negative in one patient with typical course of type B. In 1 of 4 patients with acute hepatitis A and 2 of 13 with non A non B, anti-HBc IgM (RIA and/or ELISA) was positive. These 3 patients were positive for anti-HBs at the onset of disease, so we could not made the diagnosis of acute hepatitis B. Anti-HBc IgM was positive in 21(51%) of 37 patients with HBsAg-positive chronic liver disease by RIA and in 11 (30%) by ELISA, and its cut-off index value was relatively low. These results suggest that when adequate cut-off index value is established, anti-HBc analysis is useful for differentiating recent and current infections from remote infections.     

Detection of nucleic acid lesions during photochemical inactivation of RNA viruses by treatment with methylene blue and light using real-time PCR

The mechanism of bacteriophage photoinactivation by methylene blue and light (MB+L) involves genomic RNA damage. In this study, two RNA viruses, Sindbis virus (SINV) and hepatitis C virus were treated by MB+L and their nucleic acids were amplified to show that RNA lesions occurred during inactivation. During MB+L inactivation, the viral load of both viruses was significantly reduced as MB+L exposure increased. The nucleic acid amplification of treated viral RNA was inhibited in a time-dependent manner and the percentage inhibition of amplification reached about 99% after 30 min of treatment. Furthermore, as compared to SINV viral infectivity detected by quantification of the 50% tissue culture infective dose (TCID(50)), the inhibition of SINV RNA amplification strongly correlated with a decrease in in vitro infectivity (R(2) > 0.94), suggesting that RNA serves as the main target during MB+L inactivation.