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1.
采用碱性蛋白酶对核桃蛋白进行酶解, 检测了所得酶解物的抗氧化能力, 包括对1,1-二苯基-2-三硝基苯肼(DPPH)和羟基自由基(·OH)的清除能力; 利用Sephadex G-25 凝胶层析柱和反相柱对核桃蛋白酶解物进行分离纯化; 采用液相色谱-质谱(LC-ESI-Q-TOF)联用方法测得抗氧化能力最强的多肽的序列为Ala-Gly-Gly-Ala, 其还原力和还原型谷胱甘肽相当. 相似文献
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Monirul Islam Yatao Huang Serajul Islam Bei Fan Litao Tong Fengzhong Wang 《Molecules (Basel, Switzerland)》2022,27(18)
Soybean protein hydrolysates were prepared using two proteolytic enzymes (Alcalase and Protamex) and the degree of hydrolysis (DH) and their functional and antioxidant properties were evaluated. The highest DH value was 20%, with a yield of 19.77% and protein content of 51.64%. The total amino acid content was more than 41% for all protein hydrolysates. The protein hydrolysates from Protamex at pH 2.0 had excellent solubility, emulsifying activity, and foaming capacity, at 83.83%, 95.03 m2/g, and 93.84%, respectively. The water-holding capacity was 4.52 g/g for Alcalase, and the oil-holding capacity was 4.91 g/g for Protamex. The antioxidant activity (62.07%), as measured by the samples’ reaction with DPPH (2,2-diphenyl-1-picrylhydrazyl) and the reducing power (0.27) were the strongest for Protamex. An ABTS activity rate of 70.21% was recorded for Alcalase. These findings indicated a strong potential for the utilization of soybean protein hydrolysates to improve the functional properties and antioxidant activity of soybeans as well as their nutritional values. 相似文献
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Henghui Zhang Zhijun Zhang Dongliang He Shuying Li Yongping Xu 《Molecules (Basel, Switzerland)》2022,27(3)
Botanical oils are staple consumer goods globally, but as a by-product of oil crops, meal is of low utilization value and prone to causing environmental problems. The development of proteins in meal into bioactive peptides, such as Perilla peptide, through biotechnology can not only solve environmental problems, but also create more valuable nutritional additives. In the present work, the hydrolysis process of Perilla meal protein suitable for industrial application was optimized with the response surface methodology (RSM) on the basis of single-factor experiments. Alcalase was firstly selected as the best-performing among four proteases. Then, based on Alcalase, the optimal hydrolysis conditions were as follows: enzyme concentration of 7%, hydrolysis temperature of 61.4 °C, liquid-solid ratio of 22.33:1 (mL/g) and hydrolysis time of 4 h. Under these conditions, the degree of hydrolysis (DH) of Perilla meal protein was 26.23 ± 0.83% and the DPPH scavenging capacity of hydrolysate was 94.15 ± 1.12%. The soluble peptide or protein concentration of Perilla meal protein hydrolysate rose up to 5.24 ± 0.05 mg/mL, the ideal yield of which was estimated to be 17.9%. SDS-PAGE indicated that a large proportion of new bands in hydrolysate with small molecular weights appeared, which was different from the original Perilla meal protein. The present data contributed to further, more specific research on the separation, purification and identification of antioxidant peptide from the hydrolysate of Perilla meal protein. The results showed that the hydrolysis of Perilla meal protein could yield peptides with high antioxidant activity and potential applications as natural antioxidants in the food industry. 相似文献
4.
Graciela Salete Centenaro Myriam Salas-Mellado Carla Pires Irineu Batista Maria L. Nunes Carlos Prentice 《Applied biochemistry and biotechnology》2014,172(6):2877-2893
In this work, chicken and fish peptides were obtained using the proteolytic enzymes α-Chymotrypsin and Flavourzyme. The muscle was hydrolyzed for 4 h, and the resulting peptides were evaluated. Hydrolysates were produced from Argentine croaker (Umbrina canosai) with a degree of hydrolysis (DH) of 25.9 and 27.6 % and from chicken (Gallus domesticus) with DH of 17.8 and 20.6 % for Flavourzyme and α-Chymotrypsin, respectively. Membrane ultrafiltration was used to separate fish and chicken hydrolysates from Flavourzyme and α-Chymotrypsin based on molecular weight cutoff of >1,000, <1,000 and >500, and <500 Da, to produce fractions (F1,000, F1,000–500, and F500) with antioxidant activity. Fish hydrolysates produced with Flavourzyme (FHF) and α-Chymotrypsin showed 60.8 and 50.9 % of peptides with a molecular weight of <3 kDa in its composition, respectively. To chicken hydrolysates produced with Flavourzyme and α-Chymotrypsin (CHC) was observed 83 and 92.4 % of peptides with a molecular weight of <3 kDa. The fraction that showed, in general, higher antioxidant potential was F1,000 from FHF. When added 40 mg/mL of FHF and CHC, 93 and 80 % of lipid oxidation in ground beef homogenates was inhibited, respectively. The composition of amino acids indicated higher amino acids hydrophobic content and amino acids containing sulfuric residues for FHF, which showed antioxidant potential. 相似文献
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Roger Ruan 《高等学校化学研究》2009,25(3)
The authors dealt with the effects of dynamic high pressure micro-fluidization(untreated and 20―160 MPa treated) on the structure and physico-chemical properties of egg white solutions[6%(mass fraction) or 61.2 mg protein/mL]. Micro-fluidization treatment resulted in a decrease in mean particle size,and little change in solubility. It was found that the residual denaturation enthalpy was decreased with the increase of pressure from 20 to 100 MPa and from 120 to 160 MPa,reaching into the maximum at 160 MPa. ... 相似文献
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A one-pot preparation of the chiral reducing agent diisopinocampheylchloroborane (Ipc2BCl) from α-pinene and borane methyl sulfide has been developed. The procedure obviates isolation of the air and moisture sensitive reagent, making it useful for large scale operations. Asymmetric reduction of ketones using the in situ prepared Ipc2BCl is comparable to that using isolated reagent. 相似文献
7.
A one-pot preparation of the chiral reducing agent diisopinocampheylchloroborane (Ipc2BCl) from Δ-pinene and borane methyl sulfide has been developed. The procedure obviates isolation of the air and moisture sensitive reagent, making it useful for large scale operations. Asymmetric reduction of ketones using the in situ prepared Ipc2BCl is comparable to that using isolated reagent. 相似文献
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K. P. Ravindranathan Kartha Harold J. Jennings 《Journal of carbohydrate chemistry》2013,32(5):777-781
ABSTRACT Acetobromoglycoses continue to be important as glycosyl donors in the synthesis of simple glycosides as well as complex oligosaccharides. From reducing sugars they are usually prepared via their peracetates in two steps. In the first step, sugars are converted to their peracetates using pyridine and acetic anhydride1,2 and the acetates are then converted in a second step to acetobromosugars using a solution of hydrogen bromide in glacial acetic acid(HBr/HOAc).2 Although not in use very often Redemann and Niemann3 as well as Lemieux4 have described one-pot methods for the preparation of acetobromoglucose wherein the reducing sugar is first treated with acetic anhydride in the presence of sulfuric acid3 or perchloric acid4 respectively to afford the peracetate. Direct conversion of the peracetate to its 1-bromo-derivative, in yields ranging from 80-87%, was then achieved by either treating the solution of the peracetate with gaseous HBr3 or with bromine in the presence of red phosphorus.4 In another approach to a one-pot method Humoller5 prepared tri-O-acetyl-β-L-arabinopyranosyl bromide in 40% yield by passing anhydrous HBr gas through a suspension of L-arabinose in acetic anhydride. By an essentially similar method Dale6 obtained acetobromosugars in yields ranging from 26-77% depending upon the sugar. Because acetobromosugars are the compounds most frequently used for synthesizing numerous other sugar derivatives, we thought it would be worthwhile to report our recent observation that acetic anhydride and HBr/HOAc can be used with advantage for the preparation of acetobromosugars. 相似文献
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2-substituted 1H-perimidines are readily prepared, in high yields, by refluxing a mixture of sodium pyrosulfite, an aldehyde, and 1, 8-diaminonaphthalene in aqueous ethanol or N, N-dimethylformamide. The method is of wide applicability except from araldehydes bearing a strong electron-withdrawing group (-CN, -NO2). 相似文献
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Xiyu Liao Zhenjun Zhu Shujian Wu Mengfei Chen Rui Huang Juan Wang Qingping Wu Yu Ding 《Molecules (Basel, Switzerland)》2020,25(22)
Pleurotus geesteranus is a promising source of bioactive compounds. However, knowledge of the antioxidant behaviors of P. geesteranus protein hydrolysates (PGPHs) is limited. In this study, PGPHs were prepared with papain, alcalase, flavourzyme, pepsin, and pancreatin, respectively. The antioxidant properties and cytoprotective effects against oxidative stress of PGPHs were investigated using different chemical assays and H2O2 damaged PC12 cells, respectively. The results showed that PGPHs exhibited superior antioxidant activity. Especially, hydrolysate generated by alcalase displayed the strongest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (91.62%), 2,2-azino-bis (3-ethylbenzothia zoline-6-sulfonic acid) (ABTS) radical scavenging activity (90.53%), ferric reducing antioxidant power, and metal ion-chelating activity (82.16%). Analysis of amino acid composition revealed that this hydrolysate was rich in hydrophobic, negatively charged, and aromatic amino acids, contributing to its superior antioxidant properties. Additionally, alcalase hydrolysate showed cytoprotective effects on H2O2-induced oxidative stress in PC12 cells via diminishing intracellular reactive oxygen species (ROS) accumulation by stimulating antioxidant enzyme activities. Taken together, alcalase hydrolysate of P. geesteranus protein can be used as beneficial ingredients with antioxidant properties and protective effects against ROS-mediated oxidative stress. 相似文献
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Bioluminescent Enzymatic Assay as a Tool for Studying Antioxidant Activity and Toxicity of Bioactive Compounds 下载免费PDF全文
Nadezhda S. Kudryasheva Ekaterina S. Kovel Anna S. Sachkova Anna A. Vorobeva Viktoriya G. Isakova Grigoriy N. Churilov 《Photochemistry and photobiology》2017,93(2):536-540
A bioluminescent assay based on a system of coupled enzymatic reactions catalyzed by bacterial luciferase and NADH:FMN‐oxidoreductase was developed to monitor toxicity and antioxidant activity of bioactive compounds. The assay enables studying toxic effects at the level of biomolecules and physicochemical processes, as well as determining the toxicity of general and oxidative types. Toxic and detoxifying effects of bioactive compounds were studied. Fullerenols, perspective pharmaceutical agents, nanosized particles, water‐soluble polyhydroxylated fullerene‐60 derivatives were chosen as bioactive compounds. Two homologous fullerenols with different number and type of substituents, C60O2–4(OH)20–24 and Fe0.5C60(OH) xOy (x + y = 40–42), were used. They suppressed bioluminescent intensity at concentrations >0.01 g L?1 and >0.001 g L?1 for C60O2–4(OH)20‐24 and Fe0.5C60(OH)xOy, respectively; hence, a lower toxicity of C60O2–4(OH)20–24 was demonstrated. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic oxidizers; changes in toxicities of general and oxidative type were determined; detoxification coefficients were calculated. Fullerenol C60O2–4(OH)20–24 revealed higher antioxidant ability at concentrations 10?17?10?5 g L?1. The difference in the toxicity and antioxidant activity of fullerenols was explained through their electron donor/acceptor properties and different catalytic activity. Principles of bioluminescent enzyme assay application for evaluating the toxic effect and antioxidant activity of bioactive compounds were summarized and the procedure steps were described. 相似文献
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Armin Mirzapour-Kouhdasht Marco Garcia-Vaquero Jong-Bang Eun Jesus Simal-Gandara 《Molecules (Basel, Switzerland)》2022,27(22)
This study aims to evaluate the potential in vitro antioxidant and anti-obesity activities of watermelon seed protein hydrolysates (WSPH) obtained using different combinations of enzymes alcalase–proteinase K (ALC-PK) and alcalase–actinidin (ALC-ACT). There was a direct relationship between the degree of hydrolysis (DH) and the biological activities of the WSPH, with the highest DPPH (approximately 85%) and lipase inhibitory activities (≈59%) appreciated at DH of 36–37% and 33–35% when using ALC-PK and ALC-ACT, respectively. Following molecular weight fractionation, the ALC-PK WSPH < 3 kDa (F1) assayed at 1 mg.mL−1 had the highest DPPH-radical scavenging (89.22%), ferrous chelating (FC) (79.83%), reducing power (RP) (A 0.51), lipase inhibitory (71.36%), and α-amylase inhibitory (62.08%) activities. The amino acid analysis of ALC-PK WSPH and its fractions revealed a relationship between the biological activity of the extracts and their composition. High contents of hydrophobic amino acids, arginine, and aromatic amino acids were related to high antioxidant, lipase inhibitory, and α-amylase inhibitory activities in the extracts, respectively. Overall, this study revealed that underutilized protein sources such as WSPH, using the appropriate combination of enzymes, could result in the generation of new ingredients and compounds with powerful antioxidant and anti-obesity activities with promising applications as nutraceuticals or functional foods. 相似文献
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ABSTRACT A convenient, highly efficient, one-pot, three-step procedure has been developed for the synthesis of peracetylated glycal derivatives from various reducing sugars including D-glucose, D-galactose, L-rhamnose, L-arabinose, D-maltose, D-lactose, and maltotriose. This procedure involves peracetylation of the reducing sugars with acetic anhydride and HBr/acetic acid followed by the transformation of the anomeric acetates to the corresponding bromides with additional HBr/acetic acid and finally reductive elimination of the 1-bromo and 2-acetoxy groups with Zn/CuSO4·5H2O in acetic acid/water containing sodium acetate. The overall yields of purified peracetylated glycals from the corresponding sugars range from 50 - 98%. 相似文献
18.
A High Yielding, One-Pot, Triton-B Catalyzed Synthesis of Dithiocarbamates Using Alcoholic Tosylates
Summary. A novel process for the one-step chemoselective conversion of alcoholic tosylates into dithiocarbamates as protected amines
was developed using benzyltrimethylammoniumhydroxide (Triton-B) in presence of carbon disulfide. Thus, dithiocarbamates of
different amines were prepared in very good to excellent yields. This protocol is mild, chemoselective, and efficient compared
to other reported methods.
Present address: Institute of Organic and Biomolecular Chemistry, Georg-August University, D-37077, G?ttingen, Germany 相似文献
19.
Hydrazine hydrate with acetic and propionic acids was an efficient reagent for acylation of primary and secondary amines at reflux temperatures. The reported one-pot method is high-yielding, simple, mild, and inexpensive. 相似文献