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高效液相色谱法对水果中多菌灵与福美双残留的同时测定 总被引:5,自引:0,他引:5
建立了高效液相色谱同时测定水果中多菌灵和福美双残留量的方法。样品经二氯甲烷提取,OasisHLB固相萃取柱净化后,经C18色谱柱分离,甲醇-0.05 mol/L甲酸铵溶液(60∶40,体积比)洗脱,紫外检测器进行检测。结果表明,多菌灵、福美双的的线性范围分别为0.01~10.0、0.05~10.0 mg/kg,相关系数分别为1.0、0.9999,检出限分别为0.005、0.03 mg/kg,平均加标回收率为80%~109%。该方法成功用于市售水果样品的测定。 相似文献
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高效液相色谱法测定浓缩苹果汁中的多菌灵残留量 总被引:12,自引:1,他引:12
建立了测定浓缩苹果汁中多菌灵残留量的高效液相色谱法。色谱柱为ZOBAX Extend-C_(18)柱(150mm×4.6mmi.d., 5μm),流动相为甲醇-水(体积比为60:40),流速为1.0mL、min,检测波长为285nm。多菌灵的浓度在0.05~5.00mg/L范围内与峰面积之间具有良好的线性关系,线性回归方程为A=185162c 3432.2,相关系数r=0.9998,回收率为91.8%~104.2%,相对标准偏差为7.59%~8.78%,检出限为1.0μg/L。方法简便、快速、灵敏,可满足农药残留的检测要求。 相似文献
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n RP-HPLC method for quantitative determination of chlorimuron ethyl WP in technical product and formulation was developed.Operating conditions were YWG-C18 column, 220×4. 6mm, methanol-waterethyl acetate (2:2 :1 ,volume ratio) mobile phase and UV detection at 254nm.The results showed that the recoveries were between 99.44%-99.93% and the coefficient of variation is less than 0. 16%. The method is simple,rapid and very effective. 相似文献
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浓缩刺梨汁中噻菌灵和多菌灵残留量的高效液相色谱法测定 总被引:6,自引:0,他引:6
研究了一种可同时测定浓缩刺梨汁中噻菌灵和多菌灵残留量的固相萃取-高效液相色谱法。浓缩刺梨汁样品与水按一定比例稀释后,经过调pH、离心、过滤,用混合相固相萃取小柱(Mixed—mode SPE)进行提取、净化,用配有二级管阵列检测器(DAD)的液相色谱仪检测,外标法定量。用噻菌灵和多菌灵对照品进行添加回收率测定,结果显示本方法对噻菌灵的测定低限为0.020mg/kg,回收率为77.5%~87.1%;对多菌灵的测定低限为0.020mg/kg,回收率为74.0%~96.3%;测定的相对标准偏差均不大于7.4%。本方法能满足常规噻菌灵和多菌灵残留量检测的需要。 相似文献
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高效液相色谱法测定柑橘和土壤中残留的多菌灵 总被引:3,自引:1,他引:3
建立了柑橘和土壤中多菌灵残留量的高效液相色谱(HPLC)分析方法。柑橘果肉、果皮、全果和土壤样品中残留的多菌灵用碱性乙腈溶液提取,经NH2固相萃取(SPE)柱净化,HPLC分离,紫外检测器检测,外标法定量。在0.02~5.0 mg/L范围内,多菌灵的峰面积与其质量浓度呈良好的线性关系,相关系数为0.9997。柑橘果肉、果皮、全果和土壤中添加0.05~0.5 mg/kg多菌灵标准品的平均回收率为89.2%~102%,相对标准偏差为1.8%~9.1%;柑橘果肉和土壤中多菌灵的最低检测浓度为0.05 mg/kg,柑橘果皮和全果中多菌灵的最低检测浓度为0.1 mg/kg。该方法的灵敏度、准确度和精密度均符合农药残留测定的技术要求。 相似文献
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提出了采用高效液相色谱法,使用Hypersil C18不锈钢色谱柱和乙腈-水(85+15)混合溶液为流动相进行分离,并用紫外检测器在220 nm波长处同时测定胺菊酯和右旋苯醚菊酯,此方法用作上述两杀虫剂的复合制剂0.3%胺菊酯.右旋苯醚菊酯气雾剂的质量监控分析方法。测得两化合物的相对标准偏差(n=5)依次为0.86%和2.6%,回收率依次在94.7%~105.5%和92.8%~102.9%之间。 相似文献
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用HPLC法测定五氯硝基苯和多菌灵的复配制剂 总被引:2,自引:2,他引:2
采用HPHypersilC18色谱柱,甲醇和水梯度洗脱,用二极管矩阵检测(254nm)的HPLC法,12min内同时测定了五氯硝基苯-多菌灵可湿性粉剂中2种有效成分的含量。该法简便、快速、准确,标准偏差分别为0.12%和0.15%,相对标准偏差分别为0.46%和1.44%。 相似文献
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高效液相色谱法测定金银花中保卫田残留量 总被引:7,自引:0,他引:7
本文用高效液相色谱法测定金银花保卫田残留量,使用ODS柱,流动相2%,NH4Ac-甲醇(6:4),流速1mL/min柱温30℃紫外检测器检测波长285nm,方法加标回收率80.3%~89.3%方法最低检出浓度0.02μg/g。 相似文献
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固相萃取-高效液相色谱法同时测定噻菌灵和多菌灵在浓缩菠萝汁中的残留量 总被引:3,自引:0,他引:3
建立了一种可同时测定浓缩菠萝汁中噻菌灵和多菌灵残留量的反相高效液相色谱分析法。浓缩菠萝汁样品与水按一定比例稀释后,经过调节溶液的pH值、离心、过滤,用混合相固相萃取小柱(Mixed-mode SPE)进行提取、净化,并用配有二极管阵列检测器(DAD)的液相色谱仪检测,外标法定量。使用噻菌灵和多菌灵对照品进行添加回收率测定,结果显示,本方法对噻菌灵的检出限为0.020 mg/kg,回收率为75.7%~93.3%;对多菌灵的检出限为0.020 mg/kg,回收率为80.8%~99.2%;测定的相对标准偏差均不大于5.7%。本方法简单、快速、准确,能满足常规噻菌灵和多菌灵残留量检测的需要。 相似文献
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Muhammad Zahoor 《中国化学会会志》2010,57(6):1361-1366
In this study activated carbon was used for the removal of thiram from aqueous solutions. Adsorption experiments were carried out as a function of time, initial thiram concentration and temperature. Equilibrium data fitted well to the Freundlich and Langmuir equilibrium models in the studied concentration range. Adsorption kinetics followed a pseudo second‐order kinetic model rather than pseudo first‐order model. The results from kinetic experiments were used to describe the adsorption mechanism. Both boundary layer and intraparticle diffusion played important role in the adsorption mechanism of thiram. Thermodynamic parameters (ΔG0, ΔH0, and ΔS0) were determined and the adsorption process was found to be an endothermic one. The negative values of ΔG0 at different temperatures were indicative of the spontaneity of the adsorption process. 相似文献
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《Analytical letters》2012,45(1):46-55
Abstract A Chemiluminescence Enzyme‐Linked Immuno‐Sorbent Assay (CL‐ELISA) for determination and quantification of the fungicide thiram in honeybees was developed in an indirect competitive format. The assay was optimized by determining: the optimal coating conjugate concentration and anti‐thiram antiserum dilution, the effect of the incubation time on the competitive step, the tolerance to organic solvents. The IC50 and the limit of detection (LOD) values were 60 ng mL?1 and 9 ng mL?1, respectively, similar to those of colorimetric ELISA with a calibration range of 9–15,000 ng mL?1. Cross reactivity of some related compounds such as some dithiocarbamates, a thiocarbamate, the ethylenethiourea and the tetramethylthiourea were tested. The assay was then applied to honeybees sample extracts obtained by using the liquid‐liquid extraction or the graphitized carbon‐based solid phase extraction. The calibration curves in honeybee extracts from liquid‐liquid procedure gave an IC50 of 141 ng mL?1 and a LOD of 17 ng mL?1. In case of extracts obtained by SPE these values were 139 ng mL?1 and 15 ng mL?1, respectively. The average recovery value from honeybee extracts spiked with 75 ng mL?1 of thiram was 72% for SPE, higher than for liquid‐liquid extraction (60%). On the opposite, when the honeybees were directly spiked with 2 and 10 ppm the average recovery was higher for liquid‐liquid extraction (54%), than for SPE (31%). Finally, the assay was applied to honeybee samples collected during monitoring activities in Italy and Russia. 相似文献
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《Analytical letters》2012,45(10):2055-2064
SUMMARY A simple and sensitive capillary electrophoretic method was developed for the separation and determination of Thiram in boric acid buffer by direct UV absorbance detection at λ = 254 nm. The separation behaviour of Thiram from Nabam was studied and it is dependent on pH and the nature of the buffer. The detection limit (S/N = 3) is 0.5 μg/ml for Thiram. The method was successfully applied to the analysis of wheat samples spiked with Thiram. 相似文献