多波长K系数法的改进研究

本文提出了测定混合干扰组分改进的多波长Ｋ系数法。首先采用参比波长组及测量波长组，组中各波长以吸光度的和参与Ｋ系数和△Ａ的计算，以减少测量误差；其次Ｋ系数的测定在含有所有干扰组分的溶液中进行，以减少组分吸光度非严格１００％加和引起的误差，Ｋ系数的求解使用算术平均值法，而不用量小二乘法，以减少计算误差。     

BP网络光度法测定去痛片中的四组分

应用Levenberg-Marquardt优化算法的BP网络,采用紫外分光光度法测定去痛片中四组分的含量。测定低含量组分咖啡因和苯巴比妥时,结合吸光度减法技术。氨基比林、非那西丁、咖啡因和苯巴比妥的平均回收率和RSD分别为99.3%,1.2%：;100.0%,1.1%;100.3%,1.5%;100.1%,1.3%。     

双波长K系数法同时测定食品中山梨酸和苯甲酸

本文采用双波长K系数法同时测定食品中山梨酸和苯甲酸。山梨酸和苯甲酸的线性范围分别为0～10mg／L、0～40mg／L；样品测定的精密度分别为1．3％～3．7％和1．4％～4．4％；样品加标回收率分别为96．3％～98．7％和96％～102．2％。本法快速、简便、可靠。     

K系数—多波长线性回归吸光光度法同时测定人发中铜铁锌

将双波长K系数吸光光度法与多波长线性回归吸光光度法相结合,提出一种同时测定三组分的方法,即K系数－多波长线性回归吸光光度法,采用OP－5－Br-PADAP为显色体系,控制溶液PH值为5．7,利用此方法对人发中铜、铁、锌同时测定,结果满意,相对标准偏差为0．59％－4．95％,加标加收率为95．0％－105．0％之间。方法具有简便,选择性及灵敏高的优点。     

混和组分的测定探讨—K系数—双峰双波长吸光光度法

K系数法用于消除混和组分中干扰的影响,双峰双波长法用于提高方法的灵敏度,文献早有论述,同时消除干扰和提高灵敏度,倪永年也曾有过研究,本人在前人的基础上,总结导出K系数-双峰双波长吸光光度法,方法更为简单方便,本文将对此作一     

一阶导数光谱K系数法同时测定硝基酚钠三组分

研究和建立了同时测定硝基酚钠混合三组份的新方法——一阶导数光谱K系数法。确定了最佳实验条件和测定波长,方法简便、快速、准确。通过对动植物细胞赋活增进剂中邻硝基酚钠、对硝基酚钠、5-硝基愈创木酚钠三组份的直接测定,其相对误差小于2%,相对标准偏差小于3%,回收率为99～103%。     

双波长吸光度比值K系数法同时测定三组分体系的研究与应用

本文用双波长吸光度比值Ｋ系数法同时测定撒痛风注射液中水杨酸钠，咖啡因和安替比林三组分的含量，其平均回收率及变异系数分别为１０１．５％，０．４０％；９９．９９％，０．３７％；１００．０％，０．３０％。既改善了选择性，又提高了灵敏度，弥补了常规Ｋ系数法的不足。     

多波长线性回归紫外分光光度法同时测定复方阿斯匹林片中的三组分

用改进的多波长线性回归分光光度法同时测定了复方阿期匹林片中三组分的含量,模拟样品中阿斯匹林、非那西丁、咖啡因的平均回收率分别为１００．３１％、９９．８７％、１００．７７％,相对标准偏差分别为０．３５％、０．２５％、１．３７％（ｎ＝１０）。对四个批号实际样品的测定结果与标准方法一致,适物复方阿斯匹林片的常规分析。     

多元络合物光度法测定冶炼残渣中痕量铟

本文研究了InI-4与碱性染料反应形成多元离子缔合物用于痕量铟的测定.探讨了酸度、温度及表面活性剂对形成多元配合物反应的影响,比较了单显色剂(乙基罗丹明B)与多显色剂(乙基罗丹明B-丁基罗丹明B)的测定结果,单显色剂测定竖罐炼锌焦结物和蒸馏电吸尘焦结物中铟含量的结果分别为2.49 μg/g(RSD为4.72%,n=4)和0.920 μg/g(RSD为4.94%,n=4);双显色剂体系测定竖罐炼锌焦结物和蒸馏电吸尘焦结物中铟含量的结果分别为2.50 μg/g(RSD为1.37%,n=4)和0.98 μg/g(RSD为1.73%,n=4).方法可用于冶炼残渣中铟的测定.     

主成分分析-紫外光谱法同时测定四种维生素B

采用主成分分析法(PCA)完成对多组分样品分析的建模及解析研究,用于处理紫外光谱数据,实现了维生素B_1、B_2及B_6及烟酰胺四组分的同时测定,结果可靠,操作简便。     

偏最小二乘法及主组分回归法用于药物组分的测定

本文研究了多元校准方法——偏最小二乘法(PLS)和主组份回归法(PCR)在药物多组份光度分析中的应用,获得了较满意的结果。而且在系列校准样品的实验设计、交叉证实法确定最佳因子数以及空缺组份体系的分析等方面进行了探讨。     

计算机选择波长-逆最小二乘法用于药物多组分的紫外联机测定

本文应用计算机辅助选择P矩阵法的分析波长,对三种判据进行了比较,并用于复方阿斯匹林药片中三组分的紫外联机测定。     

Applications of Spectrometric Full Spectrum Quantitation (FSQ) Software for Multicomponent Analysis and Stability Studies of Pharmaceuticals

ABSTRACT

A rapid, simple, accurate and specific spectrophotometric procedure for analysis of multi-component mixtures and for assessment of drug stability in pharmaceutical products has been described. The proposed method relies on the application of the spectrophotometric full spectrum quantification (FSQ) software to overcome severe spectral overlap and to permit measurement of the individual components in mixtures with satisfactory degree of accuracy and precision. The potential of FSQ software for multicomponent analysis was elucidated by analyzing complex mixtures of highly overlapping spectra (aspirin/caffeine/salicylic acid) and mixtures of structurally similar compounds (cephalexin/ cephradine) and (ceftriaxone/ ceftazidime /cefotaxime). Furthermore, the robustness of the FSQ for stability studies was assessed by analyzing chloramphenicol in presence of its alkali-induced degradation products and famotidine in presence of its acid-induced degradation products. The validity of FSQ software for multicomponent analysis and stability studies was evaluated by comparing the results with those of HPLC methods. Statistical analysis indicated that the FSQ and HPLC data were consistent, however the FSQ method is faster, easier and more reliable.     

Determination of Ranitidine Hydrochloride in Pharmaceutical Preparations by Ultraviolet and Visible Spectrophotometry

Abstract

Ranitidine hydrochloride in tablets (T) and injections (I) was determined by ultraviolet spectrophotometry (UVS) at 313 nm and visible spectrophotometry (VISS) at 615 nm, after reaction with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) and ferric chloride. For UVS, Beer's law was obeyed in the range 5.0 – 18.0 μg/mL. The coefficients of variation (CV) for the samples T were 0.36% and 0.71% and for the samples I were 0.51% and 0.24%. The recovery average (RA) was 99.88%. For UVS, Beer's law was observed in the range 1.44 – 5.76 μg/mL. The CV for T were 0.72% and 0.59%, and for I were 0.53% and 0.61%. The RA was 99.39%. The precision and accuracy of the two methods were compared.     

Mini Review: Determination of Sildenafil Citrate in Pharmaceutical Prepaprations

Sildenafil citrate (SC) is a commonly used pharmaceutical compound in the treatment of erectile dysfunction. Many side effects were reported for overdose including: headaches; fainting resulting from dilation of blood vessels, and blue-tinted vision. Many analytical methods are reported in literature for the assay of sildenafil citrate in its generic form or pharmaceutical formulations; these will be summarized in the present mini review.     

双硫腙萃取双波长系数倍率法测定铂和钯的研究及应用

在1mol/L盐酸溶液中,当氯化亚锡存在时,用双硫腙溶液同时萃取铂和钯,双波长系数倍率法测定。测定铂时,λ_1为600nm、λ_2为710nm,K=7,测定钯时,λ_1为720nm。λ_2为638nm,K=3.5。铂和钯均在0～1.0μg/ml浓度范围内符合比尔定律,表观摩尔吸光系数分别为2.01×10~5L·mol~(-1)·cm~(-1)和1.10×10~5L·mol~(-1)·cm~(-1)。本文还探讨了氯化亚锡在萃取反应中的作用机理。方法简便、选择性好,用于地质样品中痕量铂和钯的测定,可获得满意的结果。     

用DU-7分光光度计的多组分分析法测定复方胆汁注射液

本文报道一种测定复方胆汁注射液中三组分的方法——Beckman DU-7分光光度计的多组分分析法,平均回收率分别为100.9±0.65(苯甲酸钠)、99.26±0.71(咖啡因)和100.7±0.69(氨基比林),三批样品的测定值都与《兽药规范》法的结果很接近。