共查询到20条相似文献,搜索用时 14 毫秒
1.
Suryani Saallah Jumardi Roslan Flavian Sheryl Julius Sharinee Saallah Umi Hartina Mohamad Razali Wolyna Pindi Mohd Rosni Sulaiman Khairul Faizal Paee Siti Mazlina Mustapa Kamal 《Molecules (Basel, Switzerland)》2021,26(9)
Collagen was extracted from the body wall of sea cucumber (Holothuria scabra) using the pepsin-solubilized collagen method followed by isolation using dialysis and the ultrafiltration membrane. The yield and physicochemical properties of the collagen obtained from both isolation methods, denoted as D-PSC and UF-PSC, were compared. The ultrafiltration method affords a higher yield of collagen (11.39%) than that of the dialysis (5.15%). The isolated collagens have almost the same amino acid composition, while their functional groups, referred to as amide A, B, I, II, and III bands, were in accordance with commercial collagen, as verified by Fourier Transform Infrared (FT-IR) spectroscopy. The UV-Vis absorption peaks at 240 nm and 220 nm, respectively, indicated that the collagens produced are type-I collagen. The D-PSC showed interconnecting sheet-like fibrils, while the UF-PSC exhibited a flaky structure with flat-sheets arranged very close to each other. The higher yield and comparable physicochemical properties of the collagen obtained by ultrafiltration as compared with dialysis indicate that the membrane process has high potential to be used in large-scale collagen production for food and pharmaceutical applications. 相似文献
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Purification and Characterization of an Antibacterial Peptide from Rana Temporaria Chensinensis Skin 总被引:1,自引:0,他引:1
GOU Xiao jun LI Xiang hui DING Tian bing WANG Yong ting CHEN Huai yong HUANG Cheng fang DONG Qing chu LI Qing shan * 《高等学校化学研究》1999,(4)
IntroductionInadditiontothehighlyspecificcell-mediatedimmunesystem,vertebratesandotherorganismshaveadefensesystemmadeupofdistinctgroupsofbroad-spectrumantibacterialpeptides[1—3].Antibacterialpeptidescanbeclassifiedintotwogroupsbasedonhavingdisul-fide… 相似文献
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Enas E. Eltamany Usama Ramadan Abdelmohsen Dina M. Hal Amany K. Ibrahim Hashim A. Hassanean Reda F. A. Abdelhameed Tarek A. Temraz Dina Hajjar Arwa A. Makki Omnia Magdy Hendawy Asmaa M. AboulMagd Khayrya A. Youssif Gerhard Bringmann Safwat A. Ahmed 《Molecules (Basel, Switzerland)》2021,26(6)
Chemical investigation of the methanolic extract of the Red Sea cucumber Holothuria spinifera led to the isolation of a new cerebroside, holospiniferoside (1), together with thymidine (2), methyl-α-d-glucopyranoside (3), a new triacylglycerol (4), and cholesterol (5). Their chemical structures were established by NMR and mass spectrometric analysis, including gas chromatography–mass spectrometry (GC–MS) and high-resolution mass spectrometry (HRMS). All the isolated compounds are reported in this species for the first time. Moreover, compound 1 exhibited promising in vitro antiproliferative effect on the human breast cancer cell line (MCF-7) with IC50 of 20.6 µM compared to the IC50 of 15.3 µM for the drug cisplatin. To predict the possible mechanism underlying the cytotoxicity of compound 1, a docking study was performed to elucidate its binding interactions with the active site of the protein Mdm2–p53. Compound 1 displayed an apoptotic activity via strong interaction with the active site of the target protein. This study highlights the importance of marine natural products in the design of new anticancer agents. 相似文献
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Manickam Amsaveni Ayyappan Anumary Meiyazhagan Ashokkumar Bangaru Chandrasekaran Palanisamy Thanikaivelan 《Applied biochemistry and biotechnology》2013,171(6):1500-1512
Collagen (C) and cellulose are prominent biopolymers from the animal and plant kingdom and widely used in bioengineering. Albumin, on the other hand, is the most abundant plasma protein present in mammalian blood. In this work, collagen extracted from animal skin waste was blended with hydroxyethyl cellulose (HEC) and bovine serum albumin (A) and wet-spun to form hybrid biodegradable C/HEC/A fibers. They were further cross-linked with glutaraldehyde vapors and analyzed. X-ray diffraction and infra-red spectroscopic studies of the hybrid fibers display peaks corresponding to collagen, cellulose, and albumin. Incorporation of cellulose into the biopolymeric matrix leads to a reasonable improvement in mechanical, swelling, and thermal properties of hybrid fibers. Addition of albumin improves the regularity of fiber surface without altering the porosity as observed under a microscope. Hence, the formed hybrid biofibers can be potentially used as a suture material as well as for different biomedical applications due to their improved properties. 相似文献
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Taweesak Tangrodchanapong Nilubon Sornkaew Laphatrada Yurasakpong Nakorn Niamnont Chanin Nantasenamat Prasert Sobhon Krai Meemon 《Molecules (Basel, Switzerland)》2021,26(8)
The pathological finding of amyloid-β (Aβ) aggregates is thought to be a leading cause of untreated Alzheimer’s disease (AD). In this study, we isolated 2-butoxytetrahydrofuran (2-BTHF), a small cyclic ether, from Holothuria scabra and demonstrated its therapeutic potential against AD through the attenuation of Aβ aggregation in a transgenic Caenorhabditis elegans model. Our results revealed that amongst the five H. scabra isolated compounds, 2-BTHF was shown to be the most effective in suppressing worm paralysis caused by Aβ toxicity and in expressing strong neuroprotection in CL4176 and CL2355 strains, respectively. An immunoblot analysis showed that CL4176 and CL2006 treated with 2-BTHF showed no effect on the level of Aβ monomers but significantly reduced the toxic oligomeric form and the amount of 1,4-bis(3-carboxy-hydroxy-phenylethenyl)-benzene (X-34)-positive fibril deposits. This concurrently occurred with a reduction of reactive oxygen species (ROS) in the treated CL4176 worms. Mechanistically, heat shock factor 1 (HSF-1) (at residues histidine 63 (HIS63) and glutamine 72 (GLN72)) was shown to be 2-BTHF’s potential target that might contribute to an increased expression of autophagy-related genes required for the breakdown of the Aβ aggregate, thus attenuating its toxicity. In conclusion, 2-BTHF from H. scabra could protect C. elegans from Aβ toxicity by suppressing its aggregation via an HSF-1-regulated autophagic pathway and has been implicated as a potential drug for AD. 相似文献
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HeLI BaiLingLIU HuaLinCHEN LiZhenGAO 《中国化学快报》2003,14(11):1146-1149
Pepsin-soluble collagen was prepared from bullfrog skin and partially characterized. This study revealed interesting differences, such as molecular weight, amino acid composition,denaturation temperature (Ta), in the frog skin collagen when compared to the known vertebrate collagens. This study gives hints that bullfrog skin can be a potential, safe alternative source of collagen from cattle for use in various fields. 相似文献
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Lingzhi Kang Ying Zhou Xifang Chen Zhilian Yue Xiao Liu Chris Baker Gordon G. Wallace 《Macromolecular bioscience》2023,23(12):2300220
The development of biomimetic structures with integrated extracellular matrix (ECM) components represents a promising approach to biomaterial fabrication. Here, an artificial ECM, comprising the structural protein collagen I and elastin (ELN), as well as the glycosaminoglycan hyaluronan (HA), is reported. Specifically, collagen and ELN are electrochemically aligned to mimic the compositional characteristics of the dermal matrix. HA is incorporated into the electro-compacted collagen-ELN matrices via adsorption and chemical immobilization, to give a final composition of collagen/ELN/HA of 7:2:1. This produces a final collagen/ELN/hyaluronic acid scaffold (CEH) that recapitulates the compositional feature of the native skin ECM. This study analyzes the effect of CEH composition on the cultivation of human dermal fibroblast cells (HDFs) and immortalized human keratinocytes (HaCaTs). It is shown that the CEH scaffold supports dermal regeneration by promoting HDFs proliferation, ECM deposition, and differentiation into myofibroblasts. The CEH scaffolds are also shown to support epidermis growth by supporting HaCaTs proliferation, differentiation, and stratification. A double-layered epidermal-dermal structure is constructed on the CEH scaffold, further demonstrating its ability in supporting skin cell function and skin regeneration. 相似文献
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Nan-di Zhou Xiao-lei Gu Xiao-hong Zha Ya-ping Tian 《Applied biochemistry and biotechnology》2014,172(1):351-360
Urethanase produced by Penicillium variabile was purified through ultrasonication, concentration by polyethylene glycol 20,000, and Superdex G-200 gel filtration chromatography. The molecular weight of urethanase was determined to be around 96 kDa by gel filtration. The purified enzyme showed a single band in SDS-PAGE with the molecular weight of ~13.7 kDa, which suggests that the enzyme has a multimeric structure composed of the same subunits. Peptide map fingerprinting analysis was then carried out by MALDI/TOF-TOF MS. Within the known sequences in NCBI, glucosamine-6-phosphate deaminase and 6-phosphogluconate dehydrogenase get high score as compared with urethanase. Sequence analysis informs that N-terminal sequence of urethanase is GTNTADNDAA. The Minchaelis constant (K m) and maximum reaction rate (V m) of urethanase are 27.2 mmol/L and 156.25 μmol/L min, respectively. 相似文献
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Chemistry of Natural Compounds - The Apostichopus japonicus (Selenka) is the most valuable commercial species of sea cucumbers. The profile of triacylglycerides (TAG) from the body wall of the sea... 相似文献
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从鹰嘴豆中分离得到了三种β-半乳糖苷酶(酶Ⅰ、酶Ⅱ和酶Ⅲ)。将酶Ⅰ和酶Ⅱ进一步纯化,其比活力分别提高了19倍和48倍.酶活力回收率分别为16%和18%,测得它们的表观分子里分别为2.4×104和5.8×104;最适pH分别5.9和5.0,最适温度分别为55℃和45℃.酶Ⅰ水解ONPG和PNPG的KM分别为33×10-2mol·dm-3和60×10-3mol·dm-3;酶Ⅱ水解ONPG和PNPG的KM分别为30×10-3mol·dm-3和60×10-4mol·dm-3.乳糖和半乳糖为该酶的竞争性可逆抑制剂,棉子糖为非竞争性可逆抑制剂.该酶受Hg2+和PCMB强烈抑制和NEM明显抑制,而Mg2+、Zn2+和Ca2+具有激活作用,推知巯基(-SH)是酶活性中心必须基团. 相似文献
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Purification and Characterization of Cyclic AMP-Binding Protein from Ganoderma lucidum 总被引:1,自引:0,他引:1
WANGQi KIMJung-Sik CHUNGKi-Chul 《高等学校化学研究》2004,20(5):588-593
Cyclic AMP-binding protein was purified 30 fold from the mycelia of Ganoderma lucidum by the methods of ammonium sulfate precipitation, DEAE-cellulose, phospho-cellulose ion exchange chromatography and Sephacryl S-100 gel filtration. The molecular mass of the purified protein is 34.5 kDa and 17 kDa by Sephacryl S-100 gel filtration and SDS-ployacrylamide gel electrophoresis, respectively. From these results it is suggested that the protein has a homometric dimmer structure. The pI of the purified protein is pH 8.2 by native isoelectric focusing gel. The half-life of the protein activity in 10% glycerol at 4 ℃ is 7 d in crude extract, but its half-life is only 3 d under purifying conditions. The optimal conditions of the protein activity are at 1 ℃ and pH 7.5. Its activity is increased 6 times by 1 mmol/L Zn^2 and is slightly inhibited by cGMP,Cu^2 and Mn^2 . 相似文献
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HUANG Lu QU He-zhi ZHANG Lei DU Shan-shan YANG Shuo HAO Dong-yun WANG Xiao-ping 《高等学校化学研究》2008,24(3):348-352
Ficin is an important component of plants in Ficus family such as fig latex. It is of special significance in medicine and industry because it exhibits activity throughout a wide range of temperature and pH values. In this work, we purified a component of ficin from the latex homogeneity of Shandong fig trees, and the properties of the purified ficin were studied. The current findings revealed that heavy metal ions were able to inhibit ficin, while DTT, L-cysteine, and β-ME were found to promote ficin activity. It was also observed that the half life of ficin at 65 °C was longer than 1 h and the Michaelis constant(Km) for casein hydrolyzation was determined to be 1.56 mg/mL. Our study shows that this purified ficin is a cysteine protease. 相似文献
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The purification and characterization of intracellular tyrosine phenol lyase from Citrobacter freundii has been carried out. The enzyme was purified 35-fold to homogeneity by ammonium sulphate precipitation and hydrophobic interaction chromatography. Its subunit molecular weight was found to be 52 kDa on sodium dodecyl sulphate polyacrylamide gel electrophoresis. The purified tyrosine phenol lyase showed maximum activity in borate buffer (0.05 M at pH 8.5) at 45 °C after 20 min of incubation. The K m and V max values of purified enzyme were found to be 0.446 mm and 0.342 mM/min/mg. This enzyme exhibits t 1/2 of 10, 52 and 130 min at 55, 45 and 35 °C, respectively. The N-terminal amino acid sequence was determined as MET-ASN-TYR-PRO-ALA-GLU-PRO-PHE-ARG-ILE-TRP-TRP-VAL-GLY. 相似文献
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A New Disulfated Triterpene Glycoside from the Sea Cucumber Mensamaria intercedens Lampert 总被引:1,自引:0,他引:1
ZhengRongZOU YangHuaYI QiangZhiXU HouMingWU HouWenLIN 《中国化学快报》2003,14(6):585-587
A new disulfated triterpene glycoside named intercedenside B was isolated from the sea cucumber Memsamaria intercedens Lampert.The structure was deduced from the spectral data. 相似文献
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Nataly Retegui-Pinedo David Salirrosas Linda Snchez-Tuesta Claudio Quiones Segundo R. Juregui-Rosas Gabriela Barraza Angelita Cabrera Carmen Ayala-Jara Renata Miliani Martinez Andr Rolim Baby Zulita Adriana Prieto 《Molecules (Basel, Switzerland)》2022,27(3)
From tilapia (Oreochromis niloticus) farming, the by-products have been identified as a source of collagen that could be used for the development of dermocosmetics or pharmaceutical products. However, the characteristics of collagen related to a specific strain or culture must be well defined prior to its application. Collagen was extracted from the skin of three strains of tilapia: red YY males (YY: two Y-type sex chromosomes), XX gray females, and the F1: offspring of crossing red YY males with XX gray females; at different ages in the adult phase, using acetic acid and pepsin enzyme. The characteristics of acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were shown by SDS-PAGE band profiles to be similar to bovine collagen type I (SIGMA), the PSC of gray tilapia being more fragile to temperature changes, consistent with the results of fractional viscosity. The characteristics of the F1 progeny were prioritized for being a commercially productive and sustainable source for the extraction of collagen, and the ASC form, being the one with the greatest stability and advantage over PSC, of importance to our investigations, leads to a controlled digestion as in the case of peptide induction, and also in the development of natural products in the pharmaceutical and/or dermocosmetic industry. Evaluations of the triple helix structure by FT-IR, X-ray diffraction and UV–visible spectroscopy give similar results between the strains: red, gray, and F1, and between ages in the adult form F1 (15, 24, and 36 months of age). Consequently, the skin of tilapia in adult form is recommended sustainably for up to 24 months of age where the collagen is obtained with the use of acetic acid without enzymatic treatment. 相似文献
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ZhengRongZOU YangHuaYI HouMingWU JiuHongWU DaZhengZHOU ShuYuZHANG 《中国化学快报》2004,15(3):309-312
Two new triterpenoids named intercedenol A and B were isolated from the acid hydrolysate of the crude glycoside fraction. The structures were elucidated by ESI-MS and NMR spectrum. 相似文献
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The method for preparing low molecular weight fucosylated chondroitin sulfate from sea cucumber Isostichopus badionotus using partial acid hydrolysis was reported, and its hydrolysis mechanism was also investigated. The sea cucumber chondroitin sulfate FCS was hydrolyzed under different conditions (80 ℃ 3 h and 6 h), then isolated and purified on a Bio-P-4 geltration to prepare low molecular weight fractions (LMWF-FCS). The chemical compositions of LMWF-FCS showed the branched fucose (Fuc) was cleaved during acid hydrolysis process, whereas the mole ratio of acetyl-galactosamine (GalNAc) and glucuronic acid (GlcA) in the backbone remained the same, which indicated the backbone was a typical chondroitin sulfate structure. The disaccharide composition analysis of LMWF-FCS suggested that the sulfation patterns of GalNAc in the backbone chain changed and the substitution value was reduced. Furthermore, the 1 D NMR analysis illustrated the branched-Fuc was cleaved during acid hydrolysis, but their substitution patterns were not influenced, which was distinct from the previous reports that the substitutions of branched-Fuc in FCS were easy to change. Simultaneously, the sulfation pattern of GalNAc in backbone chain changed obviously in the acid hydrolysis process. The anticoagulant activity in vitro illuminated the anticoagulant activity of the degradation products over time in the acid hydrolysis are gradually declined, but still kept good. Therefore, the LMWF-FCS prepared could be developed as a new anticoagulant and antithrombotic drug like low molecular weight heparin. 相似文献