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1.
Doménech-Carbó A Doménech-Carbó MT Gimeno-Adelantado JV Bosch-Reig F 《Analytical and bioanalytical chemistry》2006,385(8):1552-1561
A solid-state electrochemical application of the H-point standard addition method to the quantification of two depositable
metals A and B, which produce strongly overlapped stripping peaks, is described. The method is based on the mechanical transference
of mixtures of the solid sample plus a selected compound, of a reference depositable metal R, and of known amounts of a reference
material containing A or B, to paraffin-impregnated graphite electrodes. After a reductive deposition step, voltammograms
recorded for those modified electrodes immersed into a suitable electrolyte produce stripping peaks for the oxidation of all
of the metals deposited. Measurement of the currents at selected potentials in overlapping peaks corresponding to the stripping
of A and B permits the quantitation of these metals in the solid sample, while avoiding matrix effects. The method was applied
to the simultaneous determination of Pb and Sn in archaeological glazes using PbCO3 and SnO2 as standards and ZnO as a reference material.
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2.
3.
Pérez Pavón JL García Pinto C Guerrero Peña A Moreno Cordero B 《Analytical and bioanalytical chemistry》2008,391(2):599-607
In the present work we report the results obtained with a methodology based on direct coupling of a headspace generator to
a mass spectrometer for the identification of different types of petroleum crudes in polluted soils. With no prior treatment,
the samples are subjected to the headspace generation process and the volatiles generated are introduced directly into the
mass spectrometer, thereby obtaining a fingerprint of volatiles in the sample analysed. The mass spectrum corresponding to
the mass/charge ratios (m/z) contains the information related to the composition of the headspace and is used as the analytical signal for the characterization
of the samples. The signals obtained for the different samples were treated by chemometric techniques to obtain the desired
information. The main advantage of the proposed methodology is that no prior chromatographic separation and no sample manipulation
are required. The method is rapid, simple and, in view of the results, highly promising for the implementation of a new approach
for oil spill identification in soils.
Figure PCA score plots illustrate clear discrimination of types of crude oil in polluted soil samples (e.g. results are shown for
vertisol) 相似文献
4.
Tuulia Hyötyläinen 《Analytical and bioanalytical chemistry》2009,394(3):743-758
Sample preparation before chromatographic separation is the most time-consuming and error-prone part of the analytical procedure.
Therefore, selecting and optimizing an appropriate sample preparation scheme is a key factor in the final success of the analysis,
and the judicious choice of an appropriate procedure greatly influences the reliability and accuracy of a given analysis.
The main objective of this review is to critically evaluate the applicability, disadvantages, and advantages of various sample
preparation techniques. Particular emphasis is placed on extraction techniques suitable for both liquid and solid samples.
Figure Miniaturised extraction techniques allow sensitive analysis of also small sample volumes. 相似文献
5.
Sum-frequency generation (SFG) is a nonlinear laser-spectroscopy technique suitable for analysis of adsorbed molecules. The
sub-monolayer sensitivity of SFG spectroscopy enables vibrational spectra to be obtained with high specificity for a variety
of molecules on a range of surfaces, including metals, oxides, and semiconductors. The use of ultra-short laser pulses on
time-scales of picoseconds also makes time-resolved measurements possible; this can reveal ultrafast transient changes in
molecular arrangements. This article reviews recent time-resolved SFG spectroscopy studies revealing site-hopping of adsorbed
CO on metal surfaces and the dynamics of energy relaxation at water/metal interfaces.
Time-resolved sum frequency generation spectroscopy at surfaces with non-resonant laser pulse irradiation 相似文献
6.
We report a simple method that combines dialysis, as a purification method, with the multielement capability of ICP to determine
the titanium-to-transferrin mole ratio at physiological pH, under buffer conditions. The method, by means of which titanium
and transferrin are determined simultaneously, enabled us to assess the binding capacities of different titanocene complexes.
Figure Titanocene dichloride 相似文献
7.
SPME in environmental analysis 总被引:1,自引:0,他引:1
Recent advances in the use of solid-phase microextraction (SPME) in environmental analysis, including fiber coatings, derivatization
techniques, and in-tube SPME, are reviewed in this article. Several calibration methods for SPME, including traditional calibration
methods, the equilibrium extraction method, the exhaustive extraction method, and several diffusion-based calibration methods,
are presented. Recent developed SPME devices for on-site sampling and several applications of SPME in environmental analysis
are also introduced.
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8.
Thin nanoporous alumina obtained by anodization of aluminum films offers promising advantages for application in fluorescence-based biological sensors including convenient preparation, increased density of binding sites, and improved collection efficiency of fluorescence. These advantages are illustrated in the detection of streptavidin using biotin covalently bound to the surface of alumina nanopores. Fluorescence intensity enhancement as high as 7 times is observed in nanopores in comparison to flat glass surface.
相似文献
9.
A novel quartz device has been designed to trap arsine and selenium hydride and subsequently to volatilize the collected analyte
and atomize it for atomic-absorption spectrometric detection. The device is actually the multiple microflame quartz-tube atomizer
(multiatomizer) with inlet arm modified to serve as the trap and to accommodate the oxygen-delivery capillary used to combust
hydrogen during the trapping step. The effect of relevant experimental conditions (trap temperature during trapping and hydrogen
flow rate and trap temperature during volatilization) on collection and volatilization efficiency was investigated. Under
the optimum conditions collection and volatilization efficiency for arsenic and selenium were 50 and 70%, respectively.
相似文献
10.
Hinrichs K Gensch M Esser N Schade U Rappich J Kröning S Portwich M Volkmer R 《Analytical and bioanalytical chemistry》2007,387(5):1823-1829
The standard methods currently used to read out microarrays are fluorescent and chemiluminesent imaging techniques. These
methods require labeling of a component with a marker and, usually, only the concentration of the marker molecule is detected.
A label-free imaging method that also enables quantitative spectroscopic analysis of the composition and component interaction
would be of great advantage. In this article it is shown for the first time that IR mapping ellipsometry enables label-free
imaging of a biochip before and after incubation with peptide solution. The measurements prove that IR ellipsometry is a sensitive
tool for laterally resolved identification of the different materials and determination of the composition of a biochip. The
lateral resolution required was achieved by using radiation from an infrared synchrotron beamline.
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11.
Losada J Zamora M García Armada P Cuadrado I Alonso B Casado CM 《Analytical and bioanalytical chemistry》2006,385(7):1209-1217
Amperometric bienzyme electrodes with horseradish peroxidase (HRP) and glucose oxidase (GOx) co-immobilized on polymethylferrocenyl
dendrimers deposited onto platinum electrodes have been used for determination of the hydrogen peroxide produced by the oxidase
during the enzymatic reaction. The redox dendrimers consist of flexible poly(propylenimine) dendrimer cores functionalised
with octamethylferrocenyl units. The effects of dendrimer generation, the thickness of the dendrimer layer, substrate concentration,
interferences, and reproducibility on the response of the sensors were investigated. The new bienzyme biosensors respond to
substrate at work potential values between 200 and 50 mV (vs. SCE), have good sensitivity, and are resistant to interferences.
Figure 相似文献
12.
This paper reports on an integrated analytical approach for the noninvasive characterization of Chinese nephrite samples,
encompassing both geological reference specimens and museum objects. Natural variations induced by cationic substitutions,
as well as human-induced alterations such as heating, which both affect color, are the focus of this contribution. Totally
noninvasive methods of analysis were used, including X-ray fluorescence spectroscopy, Raman microspectroscopy, visible reflectance
spectroscopy and X-ray diffraction; moreover, the feasibility of using a portable Raman spectrometer for the in-field identification
of jades has been demonstrated. Fe/Fe+Mg (% p.f.u.) ratios of the jades have been calculated based on hydroxyl stretching
Raman bands, which will provide an important addition to similar data that are being collected at major museums in the Western
and Eastern hemispheres.
相似文献
13.
Because variability exists within populations of cells, single-cell analysis has become increasingly important for probing
complex cellular environments. Capillary electrophoresis (CE) is an excellent technique for identifying and quantifying the
contents of single cells owing to its small volume requirements and fast, efficient separations with highly sensitive detection.
Recent progress in both whole-cell and subcellular sampling has allowed researchers to study cellular function in the areas
of neuroscience, oncology, enzymology, immunology, and gene expression.
相似文献
14.
Zhang Y Wu HL Xia AL Zhu SH Han QJ Yu RQ 《Analytical and bioanalytical chemistry》2006,386(6):1741-1748
In this study a new spectrofluorimetric method for the direct determination of metoprolol in human plasma is presented and
discussed. It is based on the use of fluorescence excitation–emission matrices (EEMs) and second-order calibration performed
with parallel factor analysis (PARAFAC) or alternating trilinear decomposition (ATLD). This methodology enables accurate and
reliable discrimination of the analyte signal, even in the presence of unknown and uncalibrated fluorescent component(s),
which is often referred to as the second-order advantage. No separation or sample pretreatment steps were required. Satisfactory
results were obtained. Metoprolol recoveries in plasma were determined as 87±2% and 90±4% with PARAFAC and ATLD, respectively.
All RSD values of intra- and interday assays were below 5%.
Figure A three-dimensional plot of EEMs for a plasma sample and metoprolol solution 相似文献
15.
16.
Fluoroquinolones in soil—risks and challenges 总被引:5,自引:0,他引:5
Fluoroquinolones (FQs) are among the most important antibacterial agents used in human and veterinary medicine. Because of
the growing practice of adding manure and sewage sludge to agricultural fields these drugs end up in soils, where they can
accumulate and have adverse effects on organisms. This paper presents an overview of recent developments in the determination
of FQs in solid environmental matrices and describes the risks and challenges (persistence, fate, effects, and remediation)
which result from their presence in soil.
Figure Pathways into the environment for FQs 相似文献
17.
Bonanni A Esplandiu MJ Pividori MI Alegret S del Valle M 《Analytical and bioanalytical chemistry》2006,385(7):1195-1201
Impedance spectroscopy is proposed as the transduction principle for detecting the hybridization of DNA complementary strands.
In our experiments, different DNA oligonucleotides were used as model gene substances. The gene probe is first immobilized
on a graphite-epoxy composite working electrode based genosensor. Detection principle is based on changes of impedance spectra
of a redox marker, the ferro/ferricyanide couple, after hybridization with target DNA. Resistance offered to the electrochemical
reaction serves as the working signal, allowing for an unlabelled gene assay.
相似文献
18.
Bertolini A Carelli G Francesconi F Francesconi M Marchesini L Marsili P Sorrentino F Cristoforetti G Legnaioli S Palleschi V Pardini L Salvetti A 《Analytical and bioanalytical chemistry》2006,385(2):240-247
Laser-induced breakdown spectroscopy (LIBS) is a promising technique for in situ elemental analysis. A new mobile instrument
for LIBS analysis, developed in a collaboration between Marwan Technology s.r.l. and the Applied Laser Spectroscopy Laboratory
in Pisa, is presented, and some applications of it and results from it are outlined. The innovative experimental set-up, based
on the use of two suitably retarded laser pulses and a standardless analysis procedure, which overcomes problems related to
matrix effects, greatly improves the potential of this technique for accurate quantitative analysis.
相似文献
19.
External μPIXE has been used for characterisation of small samples of varnish from historical violins, and pieces of varnished
wood from historical and modern stringed instruments. To obtain spatially resolved information about the distribution of elements
across the varnish layers single-spot analysis, line-scans, and area-mapping were performed. Local resolution of approximately
20 μm was obtained from the 3 MeV, 1 nA proton micro-probe. Results from simultaneous multi-element determination of Na, Mg,
Al, Si, P, S, K, Ca, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Br, Rb, Sr, Ag, Cd, Sn, Ba, and Pb in historical varnishes are
presented. Semi-quantitative evaluation of line-scans recorded on diverse historical varnishes is reported. The applied method
is discussed in detail and the results obtained are critically reviewed and compared with those in the literature.
相似文献
20.
Maurer HH 《Analytical and bioanalytical chemistry》2007,388(7):1315-1325
This paper reviews multi-analyte single-stage and tandem liquid chromatography–mass spectrometry (LC-MS) procedures using
different mass analyzers (quadrupole, ion trap, time-of-flight) for screening, identification, and/or quantification of drugs,
poisons, and/or their metabolites in blood, plasma, serum, or urine published after 2004. Basic information about the biosample
assayed, work-up, LC column, mobile phase, ionization type, mass spectral detection mode, and validation data of each procedure
is summarized in tables. The following analytes are covered: drugs of abuse, analgesics, opioids, sedative-hypnotics, benzodiazepines,
antidepressants including selective-serotonin reuptake inhibitors (SSRIs), herbal phenalkylamines (ephedrines), oral antidiabetics,
antiarrhythmics and other cardiovascular drugs, antiretroviral drugs, toxic alkaloids, quaternary ammonium drugs and herbicides,
and dialkylphosphate pesticides. The pros and cons of the reviewed procedures are critically discussed, particularly, the
need for studies on matrix effects, selectivity, analyte stability, and the use of stable-isotope labeled internal standards
instead of unlabeled therapeutic drugs. In conclusion, LC-MS will probably become a gold standard for detection of very low
concentrations particularly in alternative matrices and for quantification in clinical and forensic toxicology. However, some
drawbacks still need to be addressed and finally overcome.
Photos of LC-MS apparatus and typical samples suitable for toxicological analysis 相似文献