共查询到20条相似文献,搜索用时 31 毫秒
1.
Clark SC Walz JY Ducker WA 《Langmuir : the ACS journal of surfaces and colloids》2004,20(18):7616-7622
We describe the use of evanescent wave scattering to measure the separation between the surface of a solid and a particle that is attached to an atomic force microscope (AFM) cantilever. Termed evanescent wave atomic force microscopy, our approach involves measuring the intensity of the light scattered from an evanescent field formed by the total internal reflection of a laser beam at a solid/fluid interface. In a conventional AFM "colloid probe" measurement, this separation must be inferred from an examination of the surface forces. Direct measurement of this separation with an evanescent wave atomic force microscope (EW-AFM) removes some ambiguity in the surface force measurement and, in addition, allows new types of measurements. For example, the force can be monitored at a constant separation. Our evanescent scattering apparatus is essentially identical to that used in total internal reflection microscopy (TIRM), except that we collect the light that scatters back into the incident medium, because the AFM partly obscures the forward scattered light (i.e., light scattered into the transmitted region). Compared to a conventional TIRM measurement, where the particle moves freely, attaching the particle to the cantilever in an EW-AFM gives much greater control of the particle position. 相似文献
2.
《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2017,129(9):2406-2410
In this work, Fluorescent False Neurotransmitter 102 (FFN102), a synthesized analogue of biogenic neurotransmitters, was demonstrated to show both pH‐dependent fluorescence and electroactivity. To study secretory behaviors at the single‐vesicle level, FFN102 was employed as a new fluorescent/electroactive dual probe in a coupled technique (amperometry and total internal reflection fluorescence microscopy (TIRFM)). We used N13 cells, a stable clone of BON cells, to specifically accumulate FFN102 into their secretory vesicles, and then optical and electrochemical measurements of vesicular exocytosis were experimentally achieved by using indium tin oxide (ITO) transparent electrodes. Upon stimulation, FFN102 started to diffuse out from the acidic intravesicular microenvironment to the neutral extracellular space, leading to fluorescent emissions and to the electrochemical oxidation signals that were simultaneously collected from the ITO electrode surface. The correlation of fluorescence and amperometric signals resulting from the FFN102 probe allows real‐time monitoring of single exocytotic events with both high spatial and temporal resolution. This work opens new possibilities in the investigation of exocytotic mechanisms. 相似文献
3.
Eah SK Jaeger HM Scherer NF Wiederrecht GP Lin XM 《The journal of physical chemistry. B》2005,109(24):11858-11861
The spatial distribution of surface plasmon scattering from a single nanoparticle changes dramatically near a metal surface as a result of interference from the direct scattered light and indirect scattered light from the mirror reflection. The unique interference patterns have been reproduced by simulations based on Huygens-Fresnel wave propagation theory. The large spectral width of the surface plasmon scattering enables a vertical distance measurement with 10 nm resolution through this nonintrusive far field interferometry. 相似文献
4.
Fang-Fang Wang Wei Wang Xi He Lianhuan Han Jian-Zhang Zhou Zhong-Qun Tian Zhao-Wu Tian Dongping Zhan 《中国科学:化学(英文版)》2017,60(5):649-655
Scanning probe is the key issue for the electrochemical scanning probe techniques (EC-SPM) such as EC-scanning tunnel microscopy (STM), EC-atomic force microscopy (AFM) and scanning electrochemical microscopy (SECM), especially the insulative encapsulation of the nanoelectrode probe for both positioning and electrochemical feedbacks. To solve this problem, we develop a novel fabrication method of the gold nanoelectrodes: firstly, a micropipette with nanomter-sized orifice was prepared as the template by a laser puller; secondly, the inside wall of micropipette apex was blocked by compact and conic Au nano-piece through electroless plating; thirdly, the Au nano-piece was grown by bipolar electroplating and connected with a silver wire as a current collector. The fabricated Au nanoelectrode has very good voltammetric responses for the electrodic processes of both mass transfer and adsorption. The advantage lies in that it is well encapsulated by a thin glass sealing layer with a RG value lowered to 1.3, which makes it qualified in the SECM-STM coupling mode. On one hand, it can serve as STM tip for positioning which ensures the high spatial resolution; on the other hand, it is a high-quality nanoelectrode to explore the local chemical activity of the substrate. The nanofabrication method may promote the SPM techniques to obtain simultaneously the physical and chemical images with nanoscale spatial resolution, which opens a new approach to tip chemistry in electrochemical nanocatalysis and tip-enhanced spectroscopy. 相似文献
5.
A new method was developed for analyzing the normal motion of a single colloidal particle near an interface. The optical technique of total internal reflection microscopy (TIRM) was used to determine the distribution of vertical displacements of a particle from a specific starting position as a function of time. At very small displacement times, the displacements are normally distributed with a variance that is proportional to the diffusion coefficient times the displacement time. The change in the diffusion coefficient with separation distance between the particle and plate was found to match that predicted by Brenner (Chem. Eng. Sci. 16 (1961) 242). As the sampling time becomes very large, the variance reaches a constant value determined strictly by the shape of the local potential energy profile holding the particle. A major advantage of this approach, relative to other measurement methods, is that the particle's spatially variant diffusion coefficient can be determined without any knowledge of the forces acting on the particle. 相似文献
6.
《Spectrochimica Acta Part B: Atomic Spectroscopy》2001,56(11):2253-2260
This paper proposes the use of radiation scattered by the sample instead of the internal standard method for quantifying impurities in high viscosity materials using X-ray fluorescence with total reflection geometry. The method has been performed for checking trace elements in gels of polymers. Advantages include no sample preparation and a minimum amount of sample (10 μl). The method is also insensitive to instrumental variations, sample amount and particle size of the sample. The ratio of coherent to incoherent scattering intensities of X-ray was also investigated to estimate the content of C plus O in these polymers. 相似文献
7.
《Angewandte Chemie (International ed. in English)》2017,56(7):1855-1858
DNA‐based machines have attracted rapidly growing interest owing to their potential in drug delivery, biocomputing, and diagnostic applications. Herein, we report a type of exonuclease III (Exo III)‐powered stochastic DNA walker that can autonomously move on a spherical nucleic acid (SNA)‐based 3D track. The motion is propelled by unidirectional Exo III digestion of hybridized DNA tracks in a burnt‐bridge mechanism. The operation of this Exo III‐propelled DNA walker was monitored in real time and at the single‐particle resolution using total internal reflection fluorescence microscopy (TIRF). We further interrogated the morphological effect of the 3D track on the nuclease activity, which suggested that the performance of the DNA walker was critically dependent upon the DNA density and the track conformation. Finally, we demonstrated potential bioanalytical applications of this SNA‐based stochastic DNA walker by exploiting movement‐triggered cascade signal amplification. 相似文献
8.
《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2017,129(7):1881-1884
DNA‐based machines have attracted rapidly growing interest owing to their potential in drug delivery, biocomputing, and diagnostic applications. Herein, we report a type of exonuclease III (Exo III)‐powered stochastic DNA walker that can autonomously move on a spherical nucleic acid (SNA)‐based 3D track. The motion is propelled by unidirectional Exo III digestion of hybridized DNA tracks in a burnt‐bridge mechanism. The operation of this Exo III‐propelled DNA walker was monitored in real time and at the single‐particle resolution using total internal reflection fluorescence microscopy (TIRF). We further interrogated the morphological effect of the 3D track on the nuclease activity, which suggested that the performance of the DNA walker was critically dependent upon the DNA density and the track conformation. Finally, we demonstrated potential bioanalytical applications of this SNA‐based stochastic DNA walker by exploiting movement‐triggered cascade signal amplification. 相似文献
9.
Maximilian Nothaft Steffen Höhla Dr. Aurélien Nicolet Prof. Dr. Fedor Jelezko Prof. Dr. Norbert Frühauf Prof. Dr. Jens Pflaum Prof. Dr. Jörg Wrachtrup 《Chemphyschem》2011,12(14):2590-2595
Photoluminescence quenching of single dibenzoterrylene (DBT) dye molecules in a polymeric organic light‐emitting diode was utilized to analyze the current dynamics at nanometer resolution. The quenching mechanism of single DBT molecules results from an increase in the triplet‐state population induced by charge carrier recombination on individual guest molecules. As a consequence of the long triplet‐state relaxation time, its population results in a reduced photoluminescence of the dispersed fluorescent dyes. From the decrease in photoluminescence together with photon correlation measurements, we could quantify the local current density and its time‐dependent evolution in the vicinity of the single‐molecule probe. This optical technique establishes a non‐invasive approach to map the time‐resolved current density in organic light‐emitting diodes on the nanometer scale. 相似文献
10.
When a laser with 543, 668 or 790±50 nm was used to irradiate the reduced polyaniline film on an indium tin oxide electrode in hydrochloric acid, the light was scattered in all directions. The intensity of the scattered light decreased with increase in the detection angle up to the right angle. When the electrode potential was scanned between the insulating and the conducting domain, the intensity varied sigmoidally with hysteresis. The intensity increased with a decrease in the absorbance. The scattering of the light can be ascribed to multiple reflection, luminescence, or Rayleigh scattering. The spectra of the scattered light was identical with that of the incident light, suggesting the absence of luminescence. The intensity increased with an increase in thickness of the film, indicating a negligible contribution of multiple reflection. Since the volume of the oxidized film is larger than that of the reduced one, the film synthesized in the oxidized state is deformed by the electrode reduction. Then, the film density becomes locally inhomogeneous and this may give rise to the Rayleigh scattering. The potential-variation of the light scattering occurred at a more negative potential than that of the absorbance at 310 nm and of the current did. 相似文献
11.
Takuya Shimosaka Masakazu Izako Katsumi Uchiyama Toshiyuki Hobo 《Analytical sciences》2005,21(4):469-472
In scanning microscopy by total internal reflection with thermal lens spectroscopy, its spatial resolution depends on the distance between the sample and a converging lens, which corresponds to the objective lens in an ordinary optical microscope. It was found that the resolution was best when the signal induced by the thermal lens effect was maximum. The distance was precisely adjusted by monitoring the signal intensity, and the resolution became twice better than that previously reported. Using a shorter focal-length lens, a resolution of 1.9 microm was attained. 相似文献
12.
《Angewandte Chemie (International ed. in English)》2017,56(35):10598-10601
Single‐particle electrochemistry at a nanoelectrode is explored by dark‐field optical microscopy. The analysis of the scattered light allows in situ dynamic monitoring of the electrodeposition of single cobalt nanoparticles down to a radius of 65 nm. Larger sub‐micrometer particles are directly sized optically by super‐localization of the edges and the scattered light contains complementary information concerning the particle redox chemistry. This opto‐electrochemical approach is used to derive mechanistic insights about electrocatalysis that are not accessible from single‐particle electrochemistry. 相似文献
13.
Attenuated total internal reflection Fourier-transform infrared spectroscopy (ATR-FTIR) was used to probe the change in water structure in silica colloids as a function of particle density. The absorption index (k) spectra were calculated from the ATR spectra using the subtractive Kramers-Kronig transform in order to avoid the effects of the density-dependent refractive index on the raw spectra and allow direct comparison of the different chemical environments. Normalized difference spectra were obtained by subtracting the k spectrum of bulk water from those of the silica colloids. At low particle densities, these difference spectra reveal the presence of a strongly hydrogen-bonded hydration layer at the surface of the colloidal particles. At higher particle densities, the hydrogen-bonding network is increasingly disrupted. The results provide direct experimental evidence of hydrogen-bond breaking as the mechanism for the hydration force, which provides the extraordinary stability of colloidal silica. 相似文献
14.
The relation between probe size and spatial resolution of backscattered electron (BSE) images was studied. In addition, the effect of the accelerating voltage, the current intensity and the sample geometry and composition were analyzed. An image synthesis method was developed to generate the images from backscattered electron coefficients obtained from Monte Carlo simulations. Spatial resolutions of simulated images were determined with the SMART-J method, which is based on the Fourier transform of the image. The resolution can be improved by either increasing the signal or decreasing the noise of the backscattered electron image. The analyses demonstrate that using a probe size smaller than the size of the observed object (sample features) does not improve the spatial resolution. For a probe size larger than the feature size, the spatial resolution is proportional to the probe size. 相似文献
15.
The optical technique of total internal reflection microscopy was used to study the normal Brownian motion of a single colloidal particle near an interface. The measurements were made using a recently developed technique in which the diffusion coefficient was determined by the variance of the short-time (Deltat --> 0) motion of the particle. Experiments were performed in solutions containing either silica nanospheres or clay platelets (Laponite RD) to investigate the effect of nonadsorbed material on the dynamics of near-contact particle motion. The change in the diffusion coefficient with separation distance between the particle and plate in solutions containing nonadsorbed macro-ions was well-described by the theory developed for simple fluids. These results suggest that, in dilute solutions of nonadsorbed material in which the bulk rheological properties remain similar to those of the pure fluid, the mobility and diffusion coefficient correction factors developed for simple fluids remain valid. 相似文献
16.
Chen H Longfield DE Varahagiri VS Nguyen KT Patrick AL Qian H VanDerveer DG Anker JN 《The Analyst》2011,136(17):3438-3445
We report a high-spatial resolution imaging technique to measure optical absorption and detect chemical and physical changes on surfaces embedded in thick tissue. Developing sensors to measure chemical concentrations on implanted surfaces through tissue is an important challenge for analytical chemistry and biomedical imaging. Tissue scattering dramatically reduces the resolution of optical imaging. In contrast, X-rays provide high spatial resolution imaging through tissue but do not measure chemical concentrations. We describe a hybrid technique which uses a scanning X-ray beam to irradiate Gd(2)O(2)S scintillators and detect the resulting visible luminescence through the tissue. The amount of light collected is modulated by optical absorption in close proximity to the luminescence source. By scanning the X-ray beam, and measuring total amount of light collected, one can measure the local absorption near scintillators at a resolution limited by the width of luminescence source (i.e. the width of the X-ray excitation beam). For proof of principle, a rectangular 1.7 mm scanning X-ray beam was used to excite a single layer of 8 μm Gd(2)O(2)S particles, and detect the absorption of 5 nm thick silver island film through 10 mm of pork. Lifetime and spectroscopic measurements, as well changing the refractive index of the surroundings indicate that the silver reduces the optical signal through attenuated total internal reflection. The technique was used to image the dissolution of regions of the silver island film which were exposed to 1 mM of H(2)O(2) through 1 cm of pork tissue. 相似文献
17.
18.
Recent advances in optical microscopy, such as total internal reflection and confocal scanning laser techniques, now permit the direct three-dimensional tracking of large numbers of colloidal particles both near and far from interfaces. A novel application of this technology, currently being developed by one of the authors under the name of diffusing colloidal probe microscopy (DCPM), is to use colloidal particles as probes of the energetic characteristics of a surface. A major theoretical challenge in implementing DCPM is to obtain the potential energy of a single particle in the external field created by the surface, from the measured particle trajectories in a dense colloidal system. In this paper we develop an approach based on an inversion of density-functional theory (DFT), where we calculate the single-particle-surface potential from the experimentally measured equilibrium density profile in a nondilute colloidal fluid. The underlying DFT formulation is based on the recent work of Zhou and Ruckenstein [Zhou and Ruckenstein, J. Chem. Phys. 112, 8079 (2000)]. For model hard-sphere and Lennard-Jones systems, using Monte Carlo simulation to provide the "experimental" density profiles, we found that the inversion procedure reproduces the true particle-surface-potential energy to an accuracy within typical DCPM experimental limitations (approximately 0.1 kT) at low to moderate colloidal densities. The choice of DFT closures also significantly affects the accuracy. 相似文献
19.
Yuying Gao Wei Nie Qianhong Zhu Xun Wang Shengyang Wang Fengtao Fan Can Li 《Angewandte Chemie (International ed. in English)》2020,59(41):18218-18223
Controlling the interaction of polarization light with an asymmetric nanostructure such as a metal/semiconductor heterostructure provides opportunities for tuning surface plasmon excitation and near‐field spatial distribution. However, light polarization effects on interfacial charge transport and the photocatalysis of plasmonic metal/semiconductor photocatalysts are unclear. Herein, we reveal the polarization dependence of plasmonic charge separation and spatial distribution in Au/TiO2 nanoparticles under 45° incident light illumination at the single‐particle level using a combination of photon‐irradiated Kelvin probe force microscopy (KPFM) and electromagnetic field simulation. We quantitatively uncover the relationship between the local charge density and polarization angle by investigating the polarization‐dependent surface photovoltage (SPV). The plasmon‐induced photocatalytic activity is enhanced when the polarization direction is perpendicular to the Au/TiO2 interface. 相似文献
20.
A movable disc-like wire probe electrode placed inside the electrospray (ES) capillary was used to measure currents flowing within the ES device for the first time. Currents were measured between the wire probe and the ES capillary. Current maps revealing measured current versus wire probe position were generated for a variety of solution conditions in the positive and negative ion modes and are compared to potential maps. The electrospray device was found to subsist on highly stable total currents; this current regulator aspect of the ES device showed remarkable resiliency regardless of the proportion of current produced at the wire probe electrode versus the ES capillary. However, kinks observed in the current and potential maps are attributed to adsorbed air participating in electrochemical reactions, and turbulence in solution flow in the region of the Taylor cone. From differential electrospray emitter potential (DEEP) maps, current maps, and cyclic voltammetry experiments performed at different wire probe locations, evidence is provided for separate regimes of current flow in the bulk solution and in the thin "skin" of highly conductive electrolyte constituting the outer surface (air interface) of the Taylor cone. Current maps reveal that current is drawn more evenly along the length of the ES capillary when solutions are highly conductive, in agreement with previous results for DEEP maps. In less conductive solutions, the area close to the capillary exit contributes more heavily to current production. Evidence that contaminant participation in electrochemical processes occurring within the electrospray device can be largely responsible for production of the excess charge in ES droplets is also provided. These investigations complement previous DEEP mapping studies to further elucidate the details of the electrochemical processes occurring within the electrospray device. 相似文献