SERS技术研究电化学中的共吸附

ＳＥＲＳ技术研究电化学中的共吸附顾仁敖（苏州大学化学系苏州２１５００６）ＳＥＲＳＳｔｕｄｉｅｓｏｎＥｌｅｃｔｒｏｃｈｅｍｉｃａｌＣｏａｄｓｏｒｐｔｉｏｎＧｕＲｅｎａｏ（ＤｅｐａｒｔｍｅｎｔＣｈｅｍｉｓｔｒｙ，ＳｕｚｈｏｕＵｎｉｖｅｒｓｉｔｙＳｕｚｈｏ...     

ELECTRON MOMENTUM SPECTRA OF EXCITED He(2~1S) AND He(2~3S)

ＥＬＥＣＴＲＯＮＭＯＭＥＮＴＵＭＳＰＥＣＴＲＡＯＦＥＸＣＩＴＥＤＨｅ（２１Ｓ）ＡＮＤＨｅ（２３Ｓ）ＣｈｅｎＺｈａｎｇｊｉｎＳｈｉＱｉｃｕｎＣｈｅｎＪｉＸｕＫｅｚｕｎＤｅｐａｒｔｍｅｎｔｏｆＭｏｄｅｒｎＰｈｙｓｉｃｓ，ＵｎｉｖｅｒｓｉｔｙｏｆＳｃｉｅｎ...     

胆红素铬合物的表面增强拉曼光谱研究

本文通过对近红外激发傅里叶变换拉曼光谱（ＮＩＲ－ＦＴ－Ｒａｍａｎ）与表面增强拉曼散射（Ｓｕｒｆａｃｅ Ｅｎｈａｎｃｅｄ Ｒａｍａｎ Ｓｃａｔｔｅｒｉｎｇ,ＳＥＲＳ）技术的联用,没得胆红素及其络合物Ｎａ２ＢＲ,ＣａＢＲ与ＣｕＢＲ在银溶胶中的ＳＥＲＳ光谱。结果表明,这一类与胆结石密切相关的生物分子络合物具有不同的配位方式,且在银胶表面采取不同的吸附取向,并从配位化学角度初步解释了黑色结石的黑色成因。     

SERS活性体系中光吸收带电磁和化学增强机制归属的认证研究

ＳＥＲＳ活性体系中光吸收带电磁和化学增强机制归属的认证研究方炎王四海（首都师范大学综合技术研究所，北京１０００３７）ＡｓｓｉｇｎｍｅｎｔｏｆＥＭａｎｄＣＴＥｎｈａｎｃｅｍｅｎｔｓｏｎＯｐｔｉｃａｌＡｂｓｏｒｐｔｉｏｎＳｐｅｃｔｒａｏｆＣｏｌｏｉｄａｌ...     

席夫碱化合物的SERS与NIR FT-Raman比较研究

席夫碱化合物的ＳＥＲＳ与ＮＩＲＦＴ┐Ｒａｍａｎ比较研究叶勇胡继明郁鉴源＊周群＊曾云鹗（武汉大学分析测试科学系武汉４３００７２）＊（清华大学分析中心北京１０００８４）ＳＥＲＳａｎｄＦＴ┐ＲａｍａｎＳｐｅｃｔｒｏｓｃｏｐｉｃＳｔｕｄｙｏｆＳｃｈｉｆＢａｓ...     

吡啶－碘电子转移复合物的表面增强拉曼光谱（SERS）研究

吡啶－碘电子转移复合物的表面增强拉曼光谱（ＳＥＲＳ）研究马树国，吴国祯（清华大学物理系北京１０００８４）ＴｈｅＳｕｒｆａｃｅＥｎｈａｎｃｅｄＲａｍａｎＳｔｕｄｙｏｆＴｈｅｃｈａｒｇｅＴｒａｎｓｆｅｒＣｏｍｐｌｅｘｏｆＰｙｒｉｄｉｎｅ－Ｉｏｄｉｎｅｏｎ...     

表面增强拉曼散射(SERS)光谱研究含偶氮基长链脂肪酸薄膜

表面增强拉曼散射（ＳＥＲＳ）光谱研究含偶氮基长链脂肪酸薄膜吴玉清赵冰徐蔚青陶艳春吴英李伯符（吉林大学超分子结构与谱学开放实验室１３００２３）ＳｕｒｆａｃｅＥｎｈａｎｃｅｄＲａｍａｎＳｐｅｃｔｒｏｓｃｏｐｉｃＳｔｕｄｙｏｆＡｚｏｂｅｎｚｅｎｅＣｏｎｔａ...     

用Nd：YAG1064nm激发的FT－SERS技术研究染料分子罗丹明B和罗丹明610

用Ｎｄ：ＹＡＧ１０６４ｎｍ激发的ＦＴ－ＳＥＲＳ技术研究染料分子罗丹明Ｂ和罗丹明６１０胡凤霞，张敏，林书煌，方炎（首都师范大学综合所，化学系，综合所，实验中心北京１０００３７）Ｓｕｒｆａｃｅ－ＥｎｈａｎｃｅｄＲａｍａｎＳｃａｔｔｅｒｉｎｇｏｆＲｈＢａｎ...     

Analytic Subject Index to Volume BS,(1996 )

ＡｎａｌｙｔｉｃＳｕｂｊｅｃｔＩｎｄｅｘｔｏＶｏｌｕｍｅＢＳ，（１９９６）ＬＡＳＥＲＤＥＶＩＣＥＳＰＵｌｓｅｗｉｄｔｈｃｈａｒａｃｔｅｒｉｓｔｉｃｓｏｆｍｏｄｅ－ｌｏｃｋｅｄＴｉ：ｓａｐｐｈｉｒｅｌａｓｅｒｗｉｔｈｒｉｎｇｃａａｖｉｔｙＫｅｒｒ－ｌｅ...     

Analytic Subject Index to Volume B3, (1994)

Ａｎａｌｙｔｉｃ Ｓｕｂｊｅｃｔ Ｉｎｄｅｘ ｔｏ Ｖｏｌｕｍｅ Ｂ３，（１９９４）ＬＡＳＥＲＤＥＶＩＣＥＳＮｏ．Ｐａｇｅ　Ｖｅｒｔｉｃａｌ－－ｃａｖｉｔｙｓｕｒｆａｃｅ－－ｅｍｉｔｔｉｎｇｌａｓｅｒｓｆａｂｒａｃａｔｅｄｔｗｉｃｅｂｙｉｍｐｌａｎｔ...     

SERS传感器间接检测蛋白质的研究进展

蛋白质在生命体中发挥着重要的作用,蛋白质的检测对于了解生命体系及在生物医学领域都有基础性的应用价值。表面增强拉曼光谱(SERS)技术对研究化学和生物传感应用前景很有优势,是由于其高灵敏度以及很好的选择性,并且已被运用于生物分子检测,特别是在蛋白质检测方面。综述了SERS探针技术用于蛋白质检验的研究现状,如免疫胶体金技术、基于纳米材料探针的SERS研究、基于酶促技术和基于试剂技术的蛋白质研究进展。     

导电玻璃支撑的双层类脂膜表面增强拉曼散射研究

初步提出一种研究双层类脂膜表面增强拉曼散射的新颖实验方法,即以导电玻璃为成膜基底,金胶为增强体系,将电化学方法与表面增强拉曼(SERS)光谱技术相结合,在获得良好循环伏安图,保证成膜状态良好的前提下,进行SERS信号收集。利用此方法,我们得到了反映成膜过程的SERS谱图,并对此进行了试探性的解释。     

Raman spectroscopic probing of plant material using SERS

We report surface‐enhanced Raman studies on intact plant material using onion layers as a biological target, and silver nanoaggregates and silver island films as enhancing plasmonic structures. Surface‐enhanced Raman scattering (SERS) enhancement allows the detection of strong Raman signatures of chemical constituents of the surface of the onion layer such as cellulose, proteins, and flavonols. Because of long‐time incubation, SERS sensors can access the extracellular space in the inner of the layer. The location of silver nanoparticles inside the onion layer has been monitored by the SERS images collected from chemicals present in the onion and/or reporter molecules attached to the nanoparticles. Our studies show a competitive adsorption of intrinsic bio molecules of the onion layer and reporter molecules. Different spectra from different places of the layer indicate the complex heterogeneous chemical structure of the plant material. The pH‐sensitive reporter molecule para mercapto benzoic acid attached to the nanoparticles allows us to infer pH values inside the extracellular matrix of the onion layer. Copyright © 2015 John Wiley & Sons, Ltd.     

电极/水溶液界面的SERS研究Ⅱ.Ag/MgSo_4体系——一种可能的检测界面pH值的方法

电极／水溶液界面的ＳＥＲＳ研究Ⅱ．Ａｇ／ＭｇＳｏ４体系——一种可能的检测界面ｐＨ值的方法陈燕霞黄开启田中群（厦门大学化学系固体表面物理化学国家重点实验室物理化学研究所厦门３６１００５）ＳＥＲＳＳｔｕｄｉｅｓｏｆＥｌｅｃｔｒｏｄｅ／Ｅｌｅｃｔｒｏｌｙ...     

Understanding SERS of bacteria

Surface‐enhanced Raman spectroscopy (SERS) has been suggested as a powerful tool to identify bacteria, drawing from its high fingerprint (vibrational) information content, its extreme sensitivity (down to the single molecule level) and its obliviousness to the aqueous environment intrinsic to biological systems. We review here in a comparative manner the various studies that attempted to utilize SERS for this important goal in light of the work carried out by our own group over the past 10 years or so. We show that SERS has an additional major advantage, namely, it introduces a new dimension of selectivity, which, on the one hand, makes it even more suitable as an analytical tool, but on the other hand, it requires gaining control of the precise manner in which the SERS‐active metal centers are produced and brought into contact with the micro‐organism. Our emphasis in this review is on understanding the spectra in terms of the nature of the SERS‐active centers and their placement within the bacterium. On the interpretation and assignment of the spectra, we constantly keep in mind the final goal of bacteria identification. Copyright © 2008 John Wiley & Sons, Ltd.     

以PAA为模板制备SERS基底及对三聚氰胺的检测

采用热蒸镀的方法直接在多孔氧化铝(porous anodic alumina,PAA)模板上蒸镀几微米的银膜,然后在HCl溶液中溶解掉模板,得到表面具有纳米尺度规则结构的银膜作为表面增强拉曼散射(surface-en-hanced Raman spectra,SERS)基底,并在该基底上测量了吡啶溶液(0.01 mol.L-1)的增强拉曼光谱,发现平均增强因子大于105。与直接在载玻片上蒸镀的银膜相比,具有纳米尺度规则结构银膜的增强效果提高了30倍。改变激发光功率测量吡啶的拉曼光谱,和普通拉曼散射一样,增强拉曼光谱的峰值强度随激发光强度线性变化,并在该基底上测量了三聚氰胺的拉曼光谱,发现在1 mW的激发功率下对于三聚氰胺的检出限为2.5 mg.L-1。     

微流控SERS芯片及其生物传感应用

由于微流控芯片具有优异的集成性和灵活的可操作性,基于芯片上的检测方法被大量开发,发展十分迅速。其中,表面增强拉曼光谱（SERS）凭借其超高的灵敏度、独一无二的指纹谱和窄峰宽等特点成为一种广泛采用的检测手段。SERS微流控芯片集SERS检测技术与微流控芯片的优势于一体,一方面为SERS检测方法的重复性和可靠性提供了一个高效平台,另一方面推动了微流控芯片的功能拓展,在生物分子探测、细胞捕获乃至组织模拟等领域具有广阔的应用前景。本文在简要介绍SERS的原理及其生物传感应用的基础上,重点概述了SERS微流控芯片的构建及其在生物传感及检测中的应用,最后探讨了该研究方向存在的问题及发展方向。     

Tailor-made metal nanoparticles as SERS substrates

In this contribution we summarize recent experiments with the objective to generate optimized substrates for surface-enhanced Raman spectroscopy (SERS). For this purpose, the well-established laser-assisted growth technique has been applied, which relies on a precise control of the growth kinetics of supported metal nanoparticles. With this method reproducible and stable SERS substrates with tailor-made optical properties possing best field enhancements were produced for specific excitation wavelengths and detection ranges. Optimization of the SERS substrates has been achieved by stabilizing the localized surface plasmon polariton resonance (SPR) of gold nanoparticles in the vicinity of the laser wavelength of λ=647 nm and λ=785 nm used for SERS excitation. After nanoparticle preparation, SERS spectra of pyrene were obtained using naturally grown nanoparticles and nanoparticles prepared by laser-assisted growth. The most important result is that the optimized substrates prepared by laser-assisted growth exhibit a significantly higher signal-to-noise ratio as compared to naturally grown nanoparticles. They are even better than substrates whose SPR has been tuned to the excitation wavelength by an elevated temperature during preparation. Another important observation is that all SERS spectra exhibit excellent reproducibility and the substrates do not show degradation during the measurements. Finally, the SERS enhancement factors due to the optimized substrates have been estimated and are on the order of 10⁵ to 10⁶.     

Fiber‐optic SERS sensor with optimized geometry: testing and optimization

A unique, geometry‐optimized, surface‐enhanced Raman scattering (SERS) fiber‐optic sensor has been recently developed and built. Though this class of sensors can be very useful in many applications, their use is greatly hindered by the fact that their reusability can hardly be achieved because of the irreversible adsorption of the analyte molecules on the SERS‐active substrate. Different substrates have been tested on our sensor with the purpose of increasing its reusability by means of cleaning procedures or good reproducibility in manufacturing the sensor, keeping, however, the same enhancement. We show that a partial reusability of the sensor is possible using SERS‐active substrates prepared by a standard process of immobilization of silver nanoparticles with 3‐aminopropyltrimethoxysilane. We also show that a fairly good reproducibility can be achieved with a low‐cost substrate realized in a short time by depositing a layer of polyvinyl alcohol (PVA) containing silver nanoparticles on the etched fiber tip. We prove as well that measurements are possible even with nanoparticles dispersed in the analyte solution instead of using a substrate directly made on the sensor tip. Finally, we have successfully tested our sensor with some molecules cited in EFSA (European Food Safety Authority) and FDA (Food and Drug Administration) reports as molecules for which new detection methods are necessary. Copyright © 2009 John Wiley & Sons, Ltd.     

Multivariate spectral analysis of pH SERS probes for improved sensing capabilities

Adsorption of functional groups to the surface of plasmonic nanoparticles provides a platform for localised optical sensing. Over the past decade, nanoscale sensors for intracellular pH measurement based on surface‐enhanced Raman spectroscopy (SERS) have been developed. However, the approaches by which pH‐SERS measurements are made and analysed can greatly impact the precision and accuracy of pH calibration. To improve pH nanosensors, the sources of experimental variation must be determined and the data must be optimally analysed. Here we report the plasmon‐induced decarboxylation of para‐mercaptobenzoic acid (pMBA) pH reporters attached to gold nanoparticles and conclude a strong association with laser power. The detrimental decarboxylation of pMBA has profound implications on the sensitivity and reliability of the pH sensor. Decarboxylation spectral signatures map directly onto those that are typically used to record pH changes, and, hence, the greatest implication of decarboxylation of pH sensors is inaccurate or false pH reporting. Here a more robust spectral analysis for pH sensing based upon an optimal spectral region for pH calibration is presented together with a unique application of the multivariate statistical technique, principal component analysis (PCA). PCA interprets complex spectral dynamics, and by direct comparisons with the typically employed ratiometric analysis, a significant improvement in generating accurate pH sensing is demonstrated. An application of these methods in determining the pH of internalised nanosensors in macrophage cells further promotes these step changes in pH measurement methodology via the avoidance of disruptive spectral signatures that arise in real applications. Copyright © 2016 John Wiley & Sons, Ltd.