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氟固相萃取(Fluorous solid-phase extraction,FSPE)是一种基于全氟化合物之间氟-氟相互作用的固相萃取技术,通过在目标分子上进行氟标签衍生,利用高氟化固相吸附剂实现特异性的分离纯化.这一技术在有机合成、催化,以及化学和生物分离分析等诸多领域应用广泛.近年来,由于氟固相萃取和生物质谱技术之间良好的兼容性,两者联用结合的分析方法受到了研究者的广泛关注.本文在简要介绍氟固相萃取技术原理的基础之上,重点综述了其在生物质谱分析领域中的应用,并对其发展前景进行了展望. 相似文献
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静态亚临界水萃取中加入固相萃取剂对河流沉积物中4种有机氯化合物(2-氯酚、2,4,6-三氯酚、五氯酚、六氯苯)进行了同时萃取。与离线亚临界水萃取-固相萃取方法相比,分析物的萃取回收率均有提高,且受亚临界水萃取罐冷却程度的影响较小。以活性炭纤维(ACF)为固相萃取剂,在160℃时,0.050 g的ACF和4.0 mL水对0.500 g的加标沉积物样品中的2-氯酚(2-CP),2,4,6-三氯酚(246-TCP),五氯酚(PCP)和六氯苯(HCB)的萃取效率比离线亚临界水萃取-固相萃取的回收率均有所提高,萃取回收率受萃取罐冷却温度影响较小。方法特别适合于高含量的沉积物样品萃取。该方法与气相色谱检测联用,分析了长江镇江段沉积物中4种有机氯化合物的含量,结果与美国环保署(USEPA)标准方法所得的结果吻合。 相似文献
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近年国内固相萃取-色谱分析的进展 总被引:7,自引:0,他引:7
对近两年国内各个领域的学者在使用固相萃取做样品前处理的色谱分析方面的研究和应用作了综述, 这一综述包括10个部分: 1. 国内近两年有关固相萃取的综述报告;2. 固相萃取-色谱分析在水质分析中的应用;3. 固相萃取-色谱分析在奶制品和肉类食品分析中的应用;4. 固相萃取-色谱用于蔬菜和水果中有害物质分析的应用;5. 固相萃取-色谱用于粮食和其他食品中有害物质分析的应用;6. 固相萃取-色谱在血药浓度、体液及组织中有害物质分析中的应用;7. 固相萃取-色谱在药物分析中的应用;8. 固相萃取-色谱在其他使用色谱分析中的应用;9. 基质固相分散在色谱分析中的应用;10. 分子印迹SPE在样品前处理中的应用. 相似文献
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采用动态微波辅助顶空固相微萃取结合气相色谱-质谱法测定芹菜叶中的挥发性和半挥发性化学成分。对水蒸气蒸馏、顶空固相微萃取、微波辅助顶空固相微萃取、动态微波辅助顶空固相微萃取等4种不同的前处理方法进行了比较,通过气相色谱-质谱分析,分别鉴定出20,17,36,41种化学成分,主要化合物为α-月桂烯、柠檬烯、β-顺式罗勒烯、β-芹子烯和(Z)-3-己烯-1-醇等。结果表明:动态微波辅助顶空固相微萃取是一种简单、快速、易操作,无需净化步骤,消耗样品量少,对于沸点较高的半挥发性物质的萃取效果优于微波辅助顶空固相微萃取的方法,可用于分析各类植物中的挥发性和半挥发性化学成分。 相似文献
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β-Cyclodextrin-polyurethane polymer as solid phase extraction material for the analysis of carcinogenic aromatic amines 总被引:1,自引:0,他引:1
β-Cyclodextrin-polyurethane (β-CDPU) polymer was synthesized by the reaction of β-cyclodextrin with hexamethylene diisocyanate in dry dimethylformamide and used as a sorbent for the solid phase extraction (SPE) of carcinogenic aromatic amines from water. The polymers were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry and particle size analysis. The separation and quantification was performed by Liquid chromatography with RP-C18 column and diode array detection using standard arylamines for quantification. When compared with commercially available sorbents, β-CDPU gave better recovery for the aromatic amines. From the variation of pH of the extractant from 4.0 to 8.5 recovery was highest at pH 8.5. The breakthrough volume has been ascertained to be 100 ml for 200 mg of the resin. The new SPE material offers better recovery and estimation of banned arylamines. 相似文献
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固相萃取新技术研究 总被引:4,自引:0,他引:4
吴芳华 《分析测试技术与仪器》2012,18(2):114-120
固相萃取技术是一种用于样品分离、纯化、浓缩的重要的样品前处理手段。近年来,高分子材料合成技术、分子印迹技术等新技术与传统固相萃取技术相结合,衍生出众多技术改进和创新。主要对各种固相萃取新技术进行评述。 相似文献
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利用分子印迹技术预处理生物样品中头孢药物的研究 总被引:9,自引:0,他引:9
优化了头孢硫脒分子印迹聚合物的合成条件,探讨了分子印迹技术和固相萃取联用对血浆中头孢硫脒的分离富集,发现用4-乙烯基吡啶作功能单体合成的分子印迹聚合物作为固相萃取填充料,能定量吸附血浆中的头孢硫脒,并初步研究了其吸附机理。 相似文献
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Solid phase extraction (SPE) is regarded as the most effective purification method for complex matrix samples owing to its simplicity of operator, time-saving, high accuracy, and environmental friendliness. SPE technology is still affected by the high cost of commercial SPE columns and poor adsorption selectivity. Hence, the development of low-cost and highly selective adsorbents is quite challenging and demanding in SPE. In this study, a novel 3DG functionalized magnetic solid phase extraction materials was prepared based on “thiol-ene” click chemistry. The structure, morphology, thermal stability, and magnetic properties of the magnetic composites were studied by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Then the adsorption performance of composite was determined by static adsorption experiments, which showed fast binding kinetics (100 min) and good adsorption performance (Qe = 65.34 mg/g). Moreover, these magnetic nanoparticles were used as adsorbents for magnetic solid phase extraction (MSPE) and coupled with high-performance liquid chromatography (HPLC) for separation and detection of illegally added hexamethylenetetramine in vermicelli. As for practical application, the recoveries for the spiked samples in the concentration range of 8–40 μg/g were between 83.24–92.69%, and the RSD was between 0.20–2.07%. 相似文献
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金属有机骨架(MOFs)材料是近几年涌现出的一类新型多功能多孔材料,以金属离子或金属簇为配位中心,与含氧或氮的有机配体通过配位作用形成多孔骨架结构。相比于其他传统无机多孔材料,MOFs具有比表面积高、孔隙率大、热稳定性好和结构与功能多样化的特点,因而被广泛用于气体存储、催化、吸附和分离等领域。MOFs复合材料在样品预处理方面的应用引起了研究者们的极大兴趣和广泛关注。由于MOFs材料和不同功能材料如高分子聚合物、碳基材料以及磁性材料组装复合,使MOFs复合材料的性能优于原来的MOFs材料。综述了近年MOFs复合材料在样品预处理的研究应用,尤其是在固相微萃取、固相萃取以及磁性固相萃取等方面的应用。 相似文献
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结合固相萃取(SPE)盘与含支撑物的SPE柱技术,制备了一种新型的无筛板型固相萃取柱.以C18填料为例,以话梅样品为介质对其中的苯甲酸进行分析,并用传统固相萃取小柱平行比较;将SPE与HPLC-UV结合,考察了填料对简单介质中苯甲酸的最大吸附量及洗脱曲线,研究了新型SPE柱在实际应用中的分离纯化效果.结果表明,新型SPE柱对样品的吸附效果更好,规格为200 mg/3 mL的SPE柱对苯甲酸的吸附量达到0.951 mg,超过了传统柱的吸附量0.908 mg;其洗脱曲线与传统柱几乎重合;苯甲酸在1~100 mg/L浓度范围内线性关系良好, r=0.9999,用此SPE柱纯化后的样品加标回收率和相对误差分别在88.4%~102.3%和1.4%~2.9%之间. 相似文献
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Fedrizzi B Versini G Lavagnini I Badocco D Nicolini G Magno F 《Analytica chimica acta》2008,621(1):38-43
This work describes a new purge and trap gas chromatography electron impact mass spectrometry (PT-GC-EIMS) method for quantifying 3-mercaptohexan-1-ol (3-MH) and 3-mercaptohexyl acetate (3-MHA), two molecules able to characterize some wines with their tropical scents. Firstly the experimental conditions of the purge and trap extraction (sample temperature, extraction time, trap temperature, flow rate) following a multivariate approach were optimized. Then the method through the construction of the calibration curves and the establishment of the detection limits was validated. The purge and trap procedure appears faster and more sensitive than both the headspace solid phase microextraction (HS-SPME) and the solid phase extraction (SPE) procedures, reaching detection limits for the two thiols closer to their sensory thresholds. Evidence of similar performances of the three sampling methods considered was gained comparing the results relevant to same wine samples. The Theil's regression method was used for purpose of comparison. 相似文献
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A sensitive method for the separation/analysis of Co(II) was described. The β-cyclodextrin cross-linked polymer (β-CDCP) was synthesized and used as solid phase extraction material (SPE) to separate/pre-concentrate trace cobalt coupled with inductively coupled plasma mass spectrometry (ICP-MS) for the analysis of Co(II). The method was based on α-pyridylazo-β-naphthol (PAN) as the complexing agent for Co(II)-PAN at neutral condition and the adsorption behavior of Co(II)-PAN on β-CDCP was studied. Further, p-octylpolyethylene-glycolphenyl ether (Triton X-100) as environment-friendly eluant was used. The linear range, detection limit (DL) and the relative standard deviation (R.S.D.) was 5.0-160.0 ng/mL, 5.84 ng/L and 2.27% (n = 3, c = 30.0 ng/mL) respectively. The enhancement factor was 10. Moreover, the β-CDCP could be used repeatedly and offered better recovery and estimation of standard samples. 相似文献