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Elena A. Markvicheva Svetlana V. Kuptsova Tatyana Yu. Mareeva Alexander A. Vikhrov Tamara N. Dugina Svetlana M. Strukova Yury N. Belokon Konstantin A. Kochetkov Ekaterine N. Baranova Vitali P. Zubov Denis Poncelet Virinder S. Parmar Rajesh Kumar Lev D. Rumsh 《Applied biochemistry and biotechnology》2000,88(1-3):145-157
A one-step mild method for entrapping animal cells and enzymes in macroporous composite poly (N-vinyl caprolactam)-calcium alginate (PVCL-CaAlg) hydrogels is described. Some properties of immobilized enzymes, such as
thermal and storage stabilities and stability in water/organic media were investigated. Composite PVCL-CaAlg gels were successfully
applied to immobilize a number of proteases, namely, trypsin, α-chymotrypsin, carboxypeptidase B, and thrombin. Thermal stability
of the immobilized preparations obtained by entrapment in hydrogel beads allowed us to use them at 65–80†C, while the native
enzymes were completely inactivated at 50–55°C. Various applications of enzymes and cells immobilized in beads weredemonstrated.
Immobilized trypsin and carboxypeptidase B were applied to prepare human insulin from recombinant proinsulin. The hydrogel
beads with entrapped α-chymotrypsin were used in enantioselective hydrolysis of Shiff's base of D,L-phenylalanine ethyl ester
(SBPH) in acetonitrile/water medium. Thrombin immobilized in PVCL-based hydrogel films was shown to be a promising compound
for wound treatment. To prepare pure preparations of monoclonal antibodies (MAb) several hybridoma cell lines, including hybridoma
cell lines producing MAb to interleukin-2, were successfully cultivated in the hydrogel beads. 相似文献
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综述了聚N-乙烯基己内酰胺(PNVCL)及其共聚物的合成与用途研究现状,评述了不同聚合方法。指出了聚N-乙烯基己内酰胺系列聚合物在各个领域的应用前景及开发策略。 相似文献
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A simple and effective synthesis of enantiomerically pure 2-(N-aryl-, N-alkyl-aminomethyl)aziridines from (2S)-N-tritylaziridine-2-carboxylic acid methyl ester has been developed. Treating of this key ester with several primary and secondary amines in the presence of AlMe3 provided the corresponding chiral N-trityl-2-carboxamides, and their reduction performed with different reagents resulted in the formation of the expected 2-(aminomethyl)aziridines. The choice of reaction conditions allows to either keep or leave the trityl substituent in the product. Such 2-(aminoalkyl)aziridines have shown very high catalytic efficiency in the asymmetric arylation of aldehydes and in other testing asymmetric reactions. On the other hand, homochiral N-trityl-2-carboxamides are interesting building blocks for the synthesis of various biologically active compounds. 相似文献
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Alexander A. Bredikhin Zemfira A. Bredikhina Alexey V. Kurenkov Dmitry B. Krivolapov 《Tetrahedron: Asymmetry》2017,28(3):442-446
Racemic 3-(4-indolyloxy)-1,2-propanediol 2 has been effectively resolved into (S)- and (R)-enantiomers by a preferential crystallization procedure. Non-racemic (S)-2 was converted into (S)-4-(2,3-epoxypropoxy)-1H-indole (S)-4 via a Mitsunobu reaction and then into (S)-pindolol (S)-1. The crystalline (S)-1 was studied by single crystal X-ray diffraction. A large number of symmetry independent molecules (Z′ = 6) led to a weakening of the system of strong intermolecular hydrogen bonds, which combined with a loose packing (PI = 64.6%), may be the cause of the abnormally low melting point of (S)-1 as compared with rac-1. 相似文献
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Liliya I. Vagapova Julia K. Voronina Victor V. Syakaev Alexander R. Burilov Airat R. Garifzyanov Mikhail A. Pudovik 《Mendeleev Communications》2018,28(4):398-400
2-Aryl-substituted pyrrolidines containing phosphine oxide group have been obtained by the reaction of P-(4,4-diethoxybutylaminomethyl)- P,P-di-p-tolylphosphine oxide with polyatomic phenols. 相似文献