Identification of human myocardial infarction in vitro based on the frequency dependence of ultrasonic backscatter.

It has been reported previously that acute and mature myocardial infarction in dogs can be differentiated in vitro and in vivo by ultrasonic tissue characterization based on measurement of the frequency dependence of ultrasonic backscatter. To characterize human infarction with an index of the frequency dependence of backscatter that could be obtained in patients, cylindrical biopsy specimens from 7 normal regions and 12 regions of infarction of 6 fixed, explanted human hearts in 2-deg steps around their entire circumference with a 5-MHz broadband transducer were insonified. One to six consecutive transmural levels were studied for each specimen. The dependence of apparent (uncompensated for attenuation or beam width) backscatter, /B(f)/2, on frequency (f) was computed from spectral analyses of radio-frequency data as /B(f)/2 = afn, where from theoretical considerations the magnitude of n decreases as scatterer size increases. Apparent integrated backscatter was computed as the average of /B(f)/2 from 3 to 7 MHz. The average value for n for normal tissue (0.9 +/- 0.1) exceeded that for tissue from regions of infarction (0.6 +/- 0.1; p less than 0.05). Infarct manifested a significant decrease of n from epicardial to endocardial levels (epi----mid----endo: 0.9----0.7----0.2; p less than 0.05) whereas normal tissue manifested similar values for n at each transmural level (0.8----1.1----0.9; p = NS). Average integrated backscatter across all transmural levels for infarct was significantly greater than for normal tissue (-48.3 +/- 0.5 vs -53.4 +/- 0.4 dB, infarct versus normal; p less than 0.05). The presence of fibrosis was associated with smaller values of n and greater integrated backscatter.(ABSTRACT TRUNCATED AT 250 WORDS)     

Applications of the expanded basis method to study the properties of photonic crystals with frequency-dependent dielectric functions and dielectric losses

Band structures of two-dimensional photonic crystals (PCs) made of polar material with frequency-dependent dielectric constant are calculated by using an expanded basis method. The effect of the polariton gap (PG) of polar material on the photonic band gaps (PBGs) is explored. It is found that the PBGs are significantly modified near the PG, where the abrupt change of the dielectric constant appears. The feature of electromagnetic (EM) field is revealed when the frequency is located in the flat band. We display the equifrequency-surface configuration of the localized field and the spacial distributions of field. We observe the existence of several equi-ω curves for a specified frequency and the localized field distribution exhibiting various different patterns, dependent of the mode index. We also study the influence of the dielectric losses of the polar material on dispersion and find that the change of the real part of the Bloch wave vector is crucially related to the real part of the dielectric constant and the attenuated length is determined by its imaginary part.     

Differentiation between tracks and damages in SSNTD under the atomic force microscope

We have observed three-dimensional sponge-like structures as well as strips of connecting pits on the surface of the LR 115 detector after etching, which can be confused with the small tracks formed after short etching time. We have employed an atomic force microscope (AFM) to study these “damages” as well as genuine alpha tracks for short etching time. It was found that while the track and damage openings could be similar in size and shape, the depths for the damages were consistently smaller. Therefore, the depth of the pits will serve as a clear criterion to differentiate between tracks and other damages. The ability to discriminate between genuine tracks from other damages is most important for etching for short time intervals.     

Fast and high-resolution MRI on the basis of interleaved-spiral technique at 4.7 T and its application for imaging of ischemic rat brain

The interleaved-spiral magnetic resonance imaging (MRI) technique was implemented and optimized on a Bruker Biospec 47/30 scanner. The method gives rise to high-resolution images with a time saving factor of up to 8, as compared to the conventional approach. A multifunctional pulse sequence for the fast interleaved-spiral MRI was composed. These functions include spin intensity imaging, transverse relaxation timeT ₂ and apparent diffusion-weighted imaging. The method was used to obtain the dynamic responses of a rat brain during ischemia.     

Potential energy of interaction between an electron and a molecule on the basis of slater atomic orbitals

General analytical expressions are obtained for the potential energy of the interaction between an electron and a molecule and the 2^L-pole electric moments of the molecules by the Hartree-Fock-Rutan method. These formulas are used for a machine calculation of the interaction potential between an electron and hydrogen and nitrogen molecules with different electron distances from these molecules and also of the electric multipole moments of a series of diatomic molecules with L = 0, 1, 2, 3.Translated from Izvestiya Vysshikh Uchebnykh Zavedenii, Fizika, No. 3, pp. 52–56, March, 1985.     

Calculation of laser-beam diffraction in the near and far zones

The article investigates the possibility of using the method of expanding the field of a diffracted wave in free-space TEM _pl modes in order to compute the diffraction of laser radiation. The calculation algorithm for the diffraction in both the near and far zones reduces to two operations: determination of the expansion coefficients and summation of the amplitudes of the TEM _pl modes in the observation plane. A comparison is made between the theoretical curves characterizing the field distribution of the diffracted-wave field, as calculated by the proposed method, and the experimental dependences for the near and far zones.Translated from Izvestiya Vysshikh Uchebnykh Zavedenii, Radiofizika, Vol. 21, No. 11, pp. 1644–1647, November, 1978.The authors are grateful to F. A. Korolev, A. I. Kostienko, and A. I. Odintsov for discussions.     

Influence of the precision of spectral backscatter measurements on the estimation of scatterers size in cancellous bone

The goal of this study is to propose a model for the ultrasonic frequency-dependent backscatter coefficient in femoral cancellous bone. This model has been developed with success to predict backscatter in human calcaneal bone [Jenson, Ultr. Med. Biol. 2003]. A weak scattering model is used and the backscatter coefficient is expressed in terms of a Gaussian autocorrelation function of the medium. The backscatter coefficient is computed and comparison is made with experimental data for 37 specimens and for frequency ranging from 0.4 to 1.2 MHz. An excellent agreement between experimental data and predictions is found for both the magnitude and the frequency-dependence of the backscatter coefficient. Then, a nonlinear regression is performed for each specimen, and the mean trabecular thickness is estimated. Experimental data and theoretical predictions are averaged over the 37 specimens. We also find a close agreement between theoretical predictions obtained using the Gaussian autocorrelation function (scatterer size=134+/-15 microm) and the mean trabecular thickness (Tb.Th=132+/-12 microm) derived from the analysis of bone 3-D micro-architecture using high-resolution micro-tomography. However, the correlation between individual experimental and estimated Tb.Th values is moderate (R(2)=0.44). The performance of the estimator are limited mainly by two factors: interference noise due to random positioning of the scatterers and attenuation. We show that the fundamental limitation of our estimator due to the speckle noise is around 5 microm for trabecular thickness estimation. This limitation is lower than the observed biological variability which is around 30 microm and should not be a limiting factor for individual prediction. A second limitation is the tremendous attenuation encountered in highly scattering media such as cancellous bone, which results in highly damped backscatter signals. The compensation for attenuation is difficult to perform, and it may be a critical point that limits the precision of the estimator.     

A numerical method to predict the effects of frequency-dependent attenuation and dispersion on speed of sound estimates in cancellous bone

Many studies have demonstrated that time-domain speed-of-sound (SOS) measurements in calcaneus are predictive of osteoporotic fracture risk. However, there is a lack of standardization for this measurement. Consequently, different investigators using different measurement systems and analysis algorithms obtain disparate quantitative values for calcaneal SOS, impairing and often precluding meaningful comparison and/or pooling of measurements. A numerical method has been developed to model the effects of frequency-dependent attenuation and dispersion on transit-time-based SOS estimates. The numerical technique is based on a previously developed linear system analytic model for Gaussian pulses propagating through linearly attenuating, weakly dispersive media. The numerical approach is somewhat more general in that it can be used to predict the effects of arbitrary pulse shapes and dispersion relationships. The numerical technique, however, utilizes several additional assumptions (compared with the analytic model) which would be required for the practical task of correcting existing clinical databases. These include a single dispersion relationship for all calcaneus samples, a simple linear model relating phase velocity to broadband ultrasonic attenuation, and a constant calcaneal thickness. Measurements on a polycarbonate plate and 30 human calcaneus samples were in good quantitative agreement with numerical predictions. In addition, the numerical approach predicts that in cancellous bone, frequency-dependent attenuation tends to be a greater contributor to variations in transit-time-based SOS estimates than dispersion. This approach may be used to adjust previously acquired individual measurements so that SOS data recorded with different devices using different algorithms may be compared in a meaningful fashion.     

An information theoretic approach to system differentiation on the basis of statistical dependencies between subsystems

We develop an analysis of complex systems in terms of statistical correlations between the dynamics of its subsystems as a formal framework within which to understand processes of system differentiation.     

Multibeam volume acoustic backscatter imagery and reverberation measurements in the northeastern Gulf of Mexico

Multibeam volume acoustic backscatter imagery and reverberation measurements are derived from data collected in 200-m-deep waters in the northeastern Gulf of Mexico, with the Toroidal Volume Search Sonar (TVSS), a 68-kHz cylindrical sonar operated by the U.S. Navy's Coastal System Station. The TVSS's 360-degree vertical imaging plane allows simultaneous identification of multiple volume scattering sources and their discrimination from backscatter at the sea surface or the seafloor. This imaging capability is used to construct a three-dimensional representation of a pelagic fish school near the bottom. Scattering layers imaged in the mixed layer and upper thermocline are attributed to assemblages of epipelagic zooplankton. The fine scale patchiness of these scatterers is assessed with the two-dimensional variance spectra of vertical volume scattering strength images in the upper and middle water column. Mean volume reverberation levels exhibit a vertical directionality which is attributed to the volume scattering layers. Boundary echo sidelobe interference and reverberation is shown to be the major limitation in obtaining bioacoustic data with the TVSS. Because net tow and trawl samples were not collected with the acoustic data, the analysis presented is based upon comparison to previous biologic surveys in the northeastern Gulf of Mexico and reference to the bioacoustic literature.     

Numerical investigation of ultrasound reflection and backscatter measurements in cancellous bone on various receiving areas

In this study, new ultrasound reflection and backscatter measurements in cancellous bone using a membrane-type hydrophone are proposed. A membrane hydrophone made of a piezoelectric polymer film mounted on an annular frame allows an incident ultrasound wave to pass through its aperture because it has no backing material. Therefore, in measurements using the membrane hydrophone, the receiving area could be located independently from the transmitting area. In addition, the size and shape of the receiving area, which corresponded to those of the electrode deposited on the piezoelectric film, could be arranged in various ways. To investigate the validity of the proposed measurements, before bench-top experiments, the reflected and backscattered waves from cancellous bone were numerically simulated using a finite-difference time-domain method. The reflection and backscatter parameters were measured on various receiving areas, and their correlation coefficients with the structural parameters in the cancellous bone were derived. The simulated results suggested that appropriate receiving areas for the reflection and backscatter measurements could exist and that the proposed measurements could be more effective for evaluating bone properties than conventional measurements.     

Transitional zones of meaning and semantics in music and language: Reply to comments on “Towards a neural basis of processing musical semantics”

Processing of meaning is critical for language perception, and therefore the majority of research on meaning processing has focused on the semantic, lexical, conceptual, and propositional processing of language. However, music is another a means of communication, and meaning also emerges from the interpretation of musical information. This article provides a framework for the investigation of the processing of musical meaning, and reviews neuroscience studies investigating this issue. These studies reveal two neural correlates of meaning processing, the N400 and the N5 (which are both components of the event-related electric brain potential). Here I argue that the N400 can be elicited by musical stimuli due to the processing of extra-musical meaning, whereas the N5 can be elicited due to the processing of intra-musical meaning. Notably, whereas the N400 can be elicited by both linguistic and musical stimuli, the N5 has so far only been observed for the processing of meaning in music. Thus, knowledge about both the N400 and the N5 can advance our understanding of how the human brain processes meaning information.     

On the configuration of Guinier-Preston zones in Al-Ag and Al-Zn alloys

An experimental investigation of the short-range ordering in the spatial distribution of isotropic G.-P. zones in supersaturated solid solutions of Ag or Zn in Al is presented. The intensity distribution of the small-angle diffuse scattering can be satisfactorily explained by inter-zone X-ray interferences when using the data on metastable miscibility gaps determined by Gerold for Al-Ag and by Hillert for Al-Zn. The investigation was carried out for a wide interval of annealing temperatures on Al-Ag (6 at. % Ag) and on Al-Zn (11 at. % Zn) samples.The authors wish to express their gratitude to their colleagues of the Institute of Solid State Physics: J. Laek, C.Sc, for valuable discussions and for chemical analyses, V. íma for alloy preparations, and Z. iký for his assistance in the measurements and computations.     

Theoretical and phantom based investigation of the impact of sound speed and backscatter variations on attenuation slope estimation

Quantitative ultrasound features such as the attenuation slope, sound speed and scatterer size, have been utilized to evaluate pathological variations in soft tissues such as the liver and breast. However, the impact of variations in the sound speed and backscatter due to underlying fat content or fibrotic changes, on the attenuation slope has not been addressed. Both numerical and acoustically uniform tissue-mimicking experimental phantoms are used to demonstrate the impact of sound speed variations on attenuation slope using clinical real-time ultrasound scanners equipped with linear array transducers. Radiofrequency data at center frequencies of 4 and 5 MHz are acquired for the experimental and numerical phantoms respectively. Numerical phantom sound speeds between 1480 and 1600 m/s in increments of 20 m/s for attenuation coefficients of 0.3, 0.4, 0.5, 0.6, and 0.7 dB/cm/MHz are simulated. Variations in the attenuation slope when the backscatter intensity of the sample is equal, 3 dB higher, and 3 dB lower than the reference is also evaluated. The sound speed for the experimental tissue-mimicking phantoms were 1500, 1540, 1560 and 1580 m/s respectively, with an attenuation coefficient of 0.5 dB/cm/MHz. Radiofrequency data is processed using three different attenuation estimation algorithms, i.e. the reference phantom, centroid downshift, and a hybrid method. In both numerical and experimental phantoms our results indicate a bias in attenuation slope estimates when the reference phantom sound speed is higher (overestimation) or lower (underestimation) than that of the sample. This bias is introduced via a small spectral shift in the normalized power spectra of the reference and sample with different sound speeds. The hybrid method provides the best estimation performance, especially for sample attenuation coefficient values lower than that of the reference phantom. The performance of all the methods deteriorates when the attenuation coefficient of the reference phantom is lower than that of the sample. In addition, the hybrid method is the least sensitive to sample backscatter intensity variations.     

Repeated zones and Mahan cones in the angular dependence of photoemission

The intensity of photoemission from the Ta-derived d states in the layer compound 1T-TaS₂ has been measured as a function of both polar and azimuthal angle of emission at the photon energy $\text{h}\text{?}\text{ω=21.2}\text{eV}$. The light source was a high intensity He resonance lamp employing an aluminum window in place of the more conventional differentially pumped capillary system. The azimuthal dependence of the d emission displays three kinds of lobes which we refer to as “heads, chins and ears”. The heads and chins are oriented along directions close to the bifurcated lobes observed previously at $\text{h}\text{?}\text{ω=10.2}\text{eV}$. The ear lobes, however, are oriented quite differently and appear only at high polar angles, corresponding to larger relative values of the parallel wavevector. The results can be understood in terms of a repeated zone scheme in which the ears and chins (not necessarily at the same polar angle) correspond to different Mahan cones of the same optical transitions.     

Leakage and water exchange characterization of gadofosveset in the myocardium

Purpose

To determine the compartmentalization of the blood pool agent gadofosveset and the effect of its transient binding to albumin on the quantification of steady-state fractional myocardial blood volume (fMBV).

Methods

Myocardial vascular fraction measurements were simulated assuming the limiting cases (slow or fast) of two-compartment water exchange for different contrast agent injection concentrations, binding fractions, bound and free relaxivities, and true cardiac vascular fractions.fMBV was measured in five healthy volunteers (4 males, 1 female, average age 33) at 1.5 T after administration of five injections of gadofosveset. The measurements in the volunteers were retrospectively compared to measurements of fMBV after three serial injections of the ultra-small, paramagnetic iron oxide (USPIO) blood pool agent ferumoxytol in an experimental animal. The true fMBV and exchange rate of water protons in both human and animal data sets was determined by chi square minimization.

Results

Simulations showed an error in the measurement of fMBV due to partial binding of gadofosveset of less than 30%. Measured fMBV values over-estimate simulation predictions, and approach cardiac extracellular volume (22%), which suggests that the intravascular assumption may not be appropriate for the myocardium, although it may apply to more distal perfusion beds. In comparison, fMBV measured with ferumoxytol (5%, with slow water proton exchange across vascular wall) agree with published values of myocardial vascular fraction. Further comparison between myocardium relaxation rates induced by gadofosveset and by other extracellular and intravascular contrast agents showed that gadofosveset behaves like an extracellular contrast agent.

Conclusions

The distribution of the volunteer data indicates that a three-compartment model, with slow water exchange of gadofosveset and water protons between the vascular and interstitial compartments, and fast water exchange between the interstitium and the myocytes, is appropriate. The ferumoxytol measurements indicate that this USPIO is an intravascular contrast agent that can be used to quantify myocardial blood volume, with the appropriate correction for water exchange using a two-compartment water exchange model.     

Comparison between measured and simulated magnetocardiograms in a case of anterolateral myocardial infarction

Summary The MCGs measured in a case of an anterolateral myocardial infarction were compared with the corresponding simulated MCGs. The most distinctive changes in the measured MCGs were observed in the upper and lower parts of the thorax as predicted by the simulation. Paper presented at the ?IV International Workshop on Biomagnetism?, held in Rome, September 14–16, 1982.     

Anisotropy of the ultrasonic backscatter of myocardial tissue: I. Theory and measurements in vitro

This research addresses the variations in the ultrasonic backscatter from specimens consisting of a suspension of approximately aligned cylindrical scatterers in a fluid medium as a function of the angle of propagation in the sample. Predictions of the angular dependence of backscatter based on the time-domain Born approximation described by Rose and Richardson [J. H. Rose and J. M. Richardson, J. Nondestr. Eval. 3, 45-53 (1982)] were compared with experimental measurements of the backscatter from both tissue-mimicking phantoms consisting of graphite fibers suspended in gelatin and from canine myocardial tissue. The angular dependence of the backscatter was predicted and measured to be maximum for propagation perpendicular to the cylinder axes and minimum for propagation parallel to the axes. Maximum to minimum (i.e., perpendicular to parallel) changes in the integrated backscatter were predicted to be between 5 and 10 dB in the phantom. The corresponding quantity measured in both the phantom and in canine myocardial tissue was approximately 6 dB.