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The plant expression vectors pBCT2 and pBT2 were constructed with the cDNA sequence (tin2) and genomic DNA sequence (tin2i) of tomato proteinase inhibitor II gene respectively. Then the two expression vectors were transferred into tobacco via the Agrobacterium tumefaciens strain LBA4404, and transgenic tobacco plants were generated. Molecular analysis and trypsin activity assay showed that both cDNA and genomic DNA were expressed properly in the transgenic plants. Insecticidal activities in these transgenic plants indicated that transgenic tobacco plants carrying tin2i sequence were more resistant to 2-instar larvae of Heliothis armigera Hubner than those carrying tin2 sequence. Therefore the intron of tin2i sequence might be a contributor to insecticidal activity of the transgenic tobacco. 相似文献
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ZHU Huanzhang* CHEN Xiaoguang* LI Feng GONG Juli & XUE Jinglun State Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Shanghai China *The authors made equal contributions to this report. Correspondence should be addressed to Xue Jinglun 《科学通报(英文版)》2003,48(20)
Hemophilia B, a serious bleeding disorder, is an inherited X chromosome-linked disease for the deficiency or inactivity of human clotting factor Ⅸ (hFⅨ). Though factor substitution therapy has greatly improved the lives of hemophiliac patients, there are still limitations to the current treatment, which have triggered interest in alternative treatments by gene therapy[1]. Based on preclinical studies in rabbits[2], our lab had first initiated an ex vivo gene therapy clinical trial whereby a… 相似文献