槲树叶中的5种酚性成分

从槲树叶Quercus dentata Thunb中分离纯化得到五种酚性成分. 通过理化性质分析和光谱解析等手段确定其化学结构分别为: 2-新橙皮糖基-2,6-二羟基苯甲酸苯甲酯(1), 龙胆酸5-O-(6-O-没食子酰基)--D-吡喃葡萄糖苷(2), 3,5,7-三羟基色原酮-3-O--D-吡喃葡萄糖苷(3), 没食子酸(4), 儿茶素(5), 其中化合物1和3为新化合物.     

穿心莲中两个黄酮苷的波谱学研究

研究了穿心莲中抗血栓的活性成分. 应用AB-8大孔吸附树脂、聚酰胺柱色谱及薄层色谱进行分离, 应用波谱学(¹H NMR, ¹³C NMR, DQFCOSY, TOCSY, HMQC, HMBC, NOESY等)方法进行结构鉴定. 分离得到两个黄酮苷类化合物, 确定了¹H NMR, ¹³C NMR信号的全归属. 化合物1鉴定为5,4'-二羟基-7-甲氧基黄酮-6-O-β-D-葡萄糖苷, 化合物2鉴定为5,4'-二羟基-7-甲氧基黄酮-8-O-β-D-葡萄糖苷, 化合物1为首次从该植物中分得, 首次对两个化合物的碳谱和氢谱进行了全归属.     

五味子中木脂素类成分的高效液相色谱-电喷雾质谱研究

采用液相色谱-电喷雾质谱联用(HPLC-ESI-MSⁿ)技术, 对北五味子与南五味子中木脂素类成分进行了系统研究. 通过HPLC-ESI-MS技术, 获得了相应化合物的保留时间、紫外光谱和分子量等信息, 利用电喷雾多级串联质谱技术(ESI-MSⁿ), 获得了相应化合物的结构信息. 研究结果表明, 北五味子与南五味子的主要木脂素成分除5个共有成分外其它成分差异较大, 并且其共有成分含量差别较大. 在此基础上, 建立了简便、快速的北五味子与南五味子药材分析鉴定的新方法.     

New lignan glycosides from Glehnia littoralis

From the roots ethanol extract of Glehnia littoralis, two new lignan glycosides, named glehlinoside E （1） and F （2）, were obtained. Their structures were determined to be （-）-secoisolariciresinol 4-O-β-D-（6-O-feruloyl） glucopyranoside （1） and （-）- secoisolariciresinol 4-O-f-β-D-（6-O-caffeloyl） glucopyranoside （2） by analysis of their spectral data, respectively.     

地奥心血康中的两个新甾体皂苷

通过正相和反相硅胶柱层析, 从地奥心血康药粉的正丁醇萃取物中分离纯化出2个微量的新甾体皂苷, 通过MS和NMR(包括HMQC, HMBC和NOESY)等波谱解析, 结合化学降解分析将其结构鉴定为3β,26-二醇-25(R)-Δ^5,20(22)-二烯-呋甾-26-O-β-D-吡喃葡萄糖苷(1)和26-O-β-D-吡喃葡萄糖基-3β,20α,26-三醇-25(R)-Δ^5,22-二烯-呋甾-3-O-α-L-吡喃鼠李糖基(1→2)-[α-L-吡喃鼠李糖基(1→4)]-β-D-吡喃葡萄糖苷(2).     

基于微量热法的板蓝根提取方法和活性部位挑选

应用TAM Air微量热系统, 采用微量热法, 考察不同提取方法的板蓝根水煎液及其化学萃取部位在37 ℃时, 对大肠杆菌生长代谢的影响. 结合中医药理论, 分析板蓝根水煎液及其化学萃取部分的药效作用及其差异, 挑选板蓝根药材最佳的提取方法和抑菌最强的活性部位, 并采用试管稀释法求出抑菌活性最强部位的最小抑菌浓度(MIC). 结果表明微量热法可用于常用中药——板蓝根的提取方法和活性部位的挑选, 为进一步研究中药的药性基础提供了一种新的、快速灵敏的技术和方法.     

New cucurbitane-type triterpenoid glucosides from the tubers of Hemsleya amabilis with anti-tumor activity

Abstract

Two new cucurbitane-type triterpenoid glucosides, 3β,11α-dihydroxycucurbita-5,24(E)-diene-3,26-glucosides (1) and 3β,11α-dihydroxycucurbita-5,24(Z)-diene-3,27-glucosides were isolated from the tubers of Hemsleya amabilis. Their structures were elucidated by various spectroscopic techniques including IR, HRESIMS, 1D and 2D NMR. The two compounds were evaluated for their anti-tumor activity against HeLa and HepG-2 cell lines, and both displayed moderate anti-tumor activity towards HeLa cell lines with IC₅₀ values of 9.8 and 15.7?μM, respectively.     

New Pyrrolo[1,2-a]azepine Type Alkaloids from Stemona and Stichoneuron (Stemonaceae)

Summary. Three new pyrroloazepine type alkaloids, stichoneurines A and B and 6-hydroxycroomine were isolated from the lipophilic root extracts of Stichoneuron caudatum and Stemona tuberosa collected in Thailand together with the already known croomine, tuberostemonine, and tuberostemonine A. The structures were elucidated by spectroscopic methods including H/H-COSY, HMQC, and HMBC. Information on the relative stereochemistries and conformational behaviour was obtained by analysis of the NOESY spectra. The formation of pyrroloazepine alkaloids in the genus Stichoneuron is reported for the first time and supports its affiliation to the family Stemonaceae. The occurrence of two different types of alkaloids, of the tuberostemonine and croomine series, in different geographical provenances of Stemona tuberosa is of special chemosystematic interest and may contribute to a more natural species delimitation within that complex group.