腹腔镜术后特殊类型子宫肌瘤复发的相关因素分析

目的探究腹腔镜术后特殊类型子宫肌瘤复发相关的因素。方法选取2013年1月至2015年12月来广东省人民医院就诊的特殊类型子宫肌瘤患者80例为对象,其中以15例复发患者为复发组,65例未复发患者为无复发组。对比分析两组患者术后的临床资料,探讨复发相关因素。结果患者子宫大小、肌瘤数量、肌瘤大小,术后饮食,运动,性生活,心理,个人卫生是特殊类型子宫肌瘤复发的危险因素,患者饮食习惯对复发具有一定影响。结论特殊类型的子宫肌瘤患者复发率较高,临床上应加强对特殊型子宫肌瘤患者的术后护理,减少复发。     

持续静脉泵入及分次静点给予特治星治疗下呼吸道感染的效果观察

目的研究持续静脉泵入及分次静点给予特治星治疗下呼吸道感染的效果。方法选择116例下呼吸道感染患者,随机分为两组,观察组给予持续静脉泵入特治星治疗方案,对照组给予分次静脉点滴特治星治疗方案,治疗1周后观察治疗效果和安全性。结果观察组总有效率为94.83%显著高于对照组84.48%(P0.05),不良反应发生率为6.9%显著低于对照组20.69%(P0.05)。两组治疗后体温(T)、白细胞(WBC)及C反应蛋白(CRP)水平较治疗前均显著降低,观察组降低更为显著(P0.05)。两组治疗后氧分压(Pa O2)水平较治疗前均显著升高,观察组升高更为显著(P0.05)。结论持续静脉泵入能显著提高下呼吸道感染患者总有效率,降低不良反应发生率。     

大隐静脉高位结扎联合泡沫硬化剂注射治疗大隐静脉曲张的临床护理观察

目的对应用全面护理模式对患有大隐静脉曲张疾病的患者在接受大隐静脉高位结扎联合泡沫硬化剂注射治疗期间实施护理的临床效果进行研究。方法选择广州市番禺区中医院收治的88例患有大隐静脉曲张疾病的患者,随机分为对照组和治疗组,平均每组44例。采用传统大隐静脉剥脱术和常规护理模式对对照组患者实施治疗和护理;采用大隐静脉高位结扎联合泡沫硬化剂注射方式和全面护理模式对治疗组患者实施治疗和护理。结果治疗组患者大隐静脉曲张疾病的治疗手术治疗效果明显优于对照组;对大隐静脉曲张手术治疗期间的护理满意度明显高于对照组;住院治疗总时间明显短于对照组。结论应用全面护理模式对患有大隐静脉曲张疾病的患者在接受大隐静脉高位结扎联合泡沫硬化剂注射治疗期间实施护理的临床效果非常明显。     

B超定位下颈内静脉穿刺的临床应用

目的讨论B超定位下颈内静脉穿刺置管的经验。方法选择严重创伤、休克、手术时间长或术后需要长期静脉营养及肿瘤内科等患者40例的临床资料。结果超声定位下穿刺成功率100%,置管成功率为100%,无一例患者出现血管、神经损伤及气胸等并发症。结论 B超定位下颈内静脉穿刺操作简单、方便、安全,值得推广。     

预防性抗生素用于胆囊切除术对术后感染性并发症的预防作用

目的观察预防性抗生素用于胆囊切除术对术后感染性并发症的预防作用。方法选取2013年5月—2015年9月普宁市人民医院肿瘤外科收治的106例胆囊疾病患者为研究对象,按随机数字表法分为两组各53例,对照组行腹腔镜胆囊切除术治疗,观察组另行胆囊切除术及预防性抗生素治疗,观察两组围术期指标[手术时间、手术切口、术中出血量、住院时间],同时观察两组术后感染性并发症发生情况。结果观察组手术时间、手术切口、出血量、住院时间治疗指标均较对照组小,只有住院时间两组间差异显著(P0.05),其它差异均无统计学意义(P0.05)。且观察组术后感染性并发症总发生率1.89%较对照组11.32%低,但不具有统计学意义(P0.05)。结论胆囊切除术中合理使用预防性抗生素可显著减小患者住院时间,但对患者术后感染性并发症发生无显著预防作用。由于本次样本量较少,需进一步研究。     

Sortagging: A Robust and Efficient Chemoenzymatic Ligation Strategy

Bioorthogonal, chemoselective ligation methods are an essential part of the tools utilized to investigate biochemical pathways. Specifically enzymatic approaches are valuable methods in this context due to the inherent specificity of the deployed enzymes and the mild conditions of the modification reactions. One of the most common strategies is based on the transpeptidation catalyzed by sortase A derived from Staphylococcus aureus. The procedure is well established and a wide variety of applications have been published to date. Here, implementations of sortase A, which range from protein labeling using fluorescence dyes and the preparation of cyclic proteins to the modification of entire cells, are summarized. Furthermore, there is a focus on the optimization approaches established to solve the drawbacks of sortase‐mediated transpeptidation.     

A Type of Auxiliary for Native Chemical Peptide Ligation beyond Cysteine and Glycine Junctions

Native chemical ligation enables the chemical synthesis of proteins. Previously, thiol‐containing auxiliary groups have been used to extend the reaction scope beyond N‐terminal cysteine residues. However, the N‐benzyl‐type auxiliaries used so far result in rather low reaction rates. Herein, a new N^α‐auxiliary is presented. Consideration of a radical fragmentation for cleavage led to the design of a new auxiliary group which is selectively removed under mildly basic conditions (pH 8.5) in the presence of TCEP and morpholine. Most importantly and in contrast to previously described auxiliaries, the 2‐mercapto‐2‐phenethyl auxiliary is not limited to Gly‐containing sites and ligations succeed at sterically demanding junctions. The auxiliary is introduced in high yield by on‐resin reductive amination with commercially available amino acid building blocks. The synthetic utility of the method is demonstrated by the synthesis of two antimicrobial proteins, DCD‐1L and opistoporin‐2.     

甘草多糖螺旋结构的原子力显微镜研究

用原子力显微镜(AFM)对甘草多糖的微观结构进行观察, 实验结果表明, 甘草多糖主要由葡萄糖、阿拉伯糖和半乳糖组成. 甘草多糖分子的稀溶液铺展在Ni^2＋处理的云母片上, 经干燥, 乙醇固定后, 获得稳定、重复的图像. 甘草多糖分子具有高度分枝的结构, 并且糖链间形成环状、柱状或近似于螺旋状的结构. 甘草多糖链呈多股紧密的螺旋结构, 这种现象可能与该多糖中分子间的Van der Waals相互作用以及糖链间氢键缔合有关.