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1.
Fenitrooxon [O,O-dimethyl-O-(4-nitro-m-tolyl)phosphate] is the major metabolite of the organophosphorus insecticide fenitrothion, and 3-methyl-4-nitrophenol is its major degradation product. In the present study, we describe the development of an indirect competitive enzyme-linked immunosorbent assay (ELISA) for the detection of these compounds in water samples based on a group-specific polyclonal antiserum generated with a “bifunctional hapten”, which has two functions: the conventional function of producing an antibody against an antigen and a unique function of promoting the production of the antibodies in rabbit. For application to water samples, the influence of several factors such as organic solvent, pH, and detergent was studied. Under optimized conditions, the quantitative working range of the fenitrooxon ELISA was 0.71-27 ng ml−1 with a limit of detection (LOD) of 0.32 ng ml−1, and the fenitrooxon concentration giving 50% reduction of the maximum signal (IC50) was 4.2 ng ml−1. The quantitative working range of the 3-methyl-4-nitrophenol ELISA was 0.67-27 ng ml−1 with a LOD of 0.38 ng ml−1 and an IC50 of 3.7 ng ml−1. No significant matrix effect originating from the water sample (river water, tap water, purified water, and bottled water) was shown by addition of Tween 20 to the assay buffer. Water samples spiked with each of these compounds at 1, 5, 10, and 20 ng ml−1 were directly analyzed without extraction and clean-up by the proposed ELISA. The mean recovery was 100.9%, and the mean coefficient of variation (CV) was 7.7% for the fenitrooxon ELISA and for the 3-methyl-4-nitrophenol ELISA, the mean recovery was 97.6%, and the mean CV was 7.2%. The proposed ELISA allows precise and accurate determination of these compounds in water at such low levels.  相似文献   

2.
Six polyclonal antisera to chloramphenicol (CAP) were successfully raised in camels, donkeys and goats. As a comparison of sensitivity, IC50 values ranged from 0.3 ng mL−1 to 5.5 ng mL−1 by enzyme-linked immunosorbent assay (ELISA) and from 0.7 ng mL−1 to 1.7 ng mL−1 by biosensor assay. The introduction of bovine milk extract improved the sensitivity of four of the antisera by ELISA and two by biosensor assay; a reduction in sensitivity of the remaining antisera ranged by a factor of 1.1-2.6. Porcine kidney extract reduced the sensitivity of all the antisera by a factor ranging from 1.1 to 7 by ELISA and a factor of 1.5 to 4 by biosensor. A low cross-reactivity with thiamphenicol (TAP) and florfenicol (FF) was displayed by antiserum G2 (1.2% and 18%, respectively) when a homologous ELISA assay format was employed. No cross-reactivity was displayed by any of the antisera when a homologous biosensor assay format was employed. Switching to a heterologous ELISA format prompted three of the antisera to display more significant cross-reactivity with TAP and FF (53% and 82%, respectively, using D1). The heterologous biosensor assay also increased the cross-reactivity of D1 for TAP and FF (56% and 129%, respectively) and of one other antiserum (G1) to a lesser degree. However, unlike the ELISA, the heterologous biosensor assay produced a substantial reduction in sensitivity (by a factor of 6 for D1).  相似文献   

3.
Based on direct hapten coated format a competitive indirect enzyme-linked immunosorbent assay (ciELISA) for bisphenol A (BPA) was developed. Polystyrene surface was modified by 3-Aminopropyltriethoxysilane (APTES) to produce amino groups after H2SO4/HNO3-pre-treatment. 4,4-bis (4-hydroxyphenyl) valeric acid (BVA) which is analogue of BPA, was successfully immobilized on the surface of microtiter plates by N,N′-dicyclohexylcarbodiimide (DCC) method. The essential steps of the assay were optimized, especially blocking procedure which is key step to prevent unspecific binding of antibody. The results indicated that compared with hapten-protein coated format (IC50 = 176.67 ng ml−1, LOD = 15.90 ng ml−1), the direct hapten coated format (IC50 = 23.50 ng ml−1, LOD = 0.27 ng ml−1) could improve assay sensitivity and the detection ranges were 2.30 ng∼157.60 ng ml−1 with good signal reproducibility (P value > 0.05) after careful optimization of assay conditions. Tap water samples and seawater samples were spiked with a known amount of BPA and measured by ciELISA. The average recoveries were between 70 and 142%. As far as we are aware this is the most sensitive ELISA for BPA yet reported.  相似文献   

4.
Three immunoassay systems: indirect, direct competitive enzyme-linked immunosorbent assay (IC-ELISA and DC-ELISA), fluorescence polarization immunoassay (FPIA) based on monoclonal antibodies for the detection of parathion-methyl (PM) were developed and optimized. Several PM derivatives (haptens) were conjugated to proteins and fluoresceinthiocarbamyl ethylenediamine (EDF) to obtain immunogens and competitors. The influence of immunogen and competitor structures on the assay performance was investigated. IC-ELISA was the most sensitive of all techniques developed, with a detection limit of 0.08 ng ml−1, but assay time was the longest (3.5 h per 96-well microtitre plate). DC-ELISA was easier to perform and quicker (1.5 h per 96-well microtitre plate) but less sensitive than IC-ELISA (detection limit was 0.5 ng ml−1). FPIA was the fastest and simplest (7 min per 10 samples) but the least sensitive (detection limit was 15 ng ml−1) technique. The methods were characterized by high specificity and reproducibility. The cross-reactivity for parathion-ethyl was around 30-40% for IC-ELISA and FPIA, but significantly higher (125%) for DC-ELISA. The immunoassays were applied to the analysis of PM residues in different food and environmental matrices. Methanol extracts of vegetable, fruit and soil samples were used for the analysis. Recoveries for most spiked samples averaged between 85 and 110%. The methods developed can be used for screening of food and environmental samples for PM residues without complicated clean-up.  相似文献   

5.
Two novel enzyme immunoassays of fentanyl have been developed using Horseradish Peroxidase (HRP) as an enzyme, 3,3′,5,5′tetramethylbenzidine (TMB) and luminol as its colorimetric and its chemiluminescence substrate, respectively. A fentanyl polyclonal antibody was used as a capture antibody for fentanyl and fentanyl-bovine serum albumin (BSA) conjugate. The latter was first biotinylated and then bound by streptavidin labeled with HRP, resulting in the development of two novel competitive immunoassays. The detection limits were 0.045 and 0.0048 ng ml−1 for spectrophotometric and chemiluminescence detection, respectively, and were much lower than existing HRP-fentanyl based kits. Intra- and inter-assay CVs were 2.6-4.5 and 5.4-11.2%, respectively, at concentrations of 0.050-5.000 ng ml−1 for the colorimetric assay, whilst for the chemiluminescent assay intra- and inter-assay CVs were 3.7-6.2 and 6.2-12.3%, respectively, for the linear range of the assay at concentrations of 0.008-0.800 ng ml−1. The methods in this study were developed in order to measure maternal and neonatal fentanyl plasma samples during cesarean section, after the application of a novel subarachnoid analgesia technique. The 28 maternal and neonatal plasma samples were measured by both assays, providing data for subarachnoid administration of fentanyl that had never been presented before.  相似文献   

6.
Yongqiang Cheng  Yuqin Su 《Talanta》2007,71(4):1757-1761
A novel assay of DNA has been proposed by using ferric nanoparticles as probes coupled with resonance light scattering (RLS) detection. At pH 7.40, the RLS intensity of ferric nanoparticles can be greatly enhanced by the aggregation of positively charged ferric nanoparticles through electrostatic interaction with negatively charged DNA. The enhanced intensity of RLS at 452 nm is proportional to the concentration of DNA in the range of 0.01-0.8 μg ml−1 for calf thymus and salmon sperm DNA and in the range of 0.005-0.3 μg ml−1 for E. coli K12 genomic DNA. Detection limits are 3.6 ng ml−1 for calf thymus DNA, 4.4 ng ml−1 for salmon sperm DNA, and 1.9 ng ml−1 for E. coli K12 genomic DNA, respectively. Compared with the chromophores previously used in RLS assay, the ferric nanoparticles have offered several advantages in easy preparation, good photostability and high sensitivity without being modified or functionalized.  相似文献   

7.
Zhong H  Li N  Zhao F  Li KA 《Talanta》2004,62(1):37-42
A new protein determination method by enhanced Rayleigh light scattering (RLS) technique has been developed. In acid condition (pH=3.60), RLS of 1,2-dihydroxyanthraquinone-3-sulfonate (Alizarin Red S) can be greatly enhanced by addition of proteins, resulting in two characteristic peaks, 360 and 505 nm, respectively. The new protein assay is based on the RLS enhancement and spectrum change. The optimum condition for the reaction was investigated. The linear range is 0.20-24.9 μg ml−1 for BSA and 0.20-15.5 μg ml−1 for HSA. The detection limits (S/N=3) are 9.59 ng ml−1 for BSA and 9.51 ng ml−1 for HSA. The results of determination for human serum samples were comparable to those obtained by Bradford method. The binding stoichiometry was determined.  相似文献   

8.
A simple and sensitive kinetic method for the determination of traces of mercury (70-760 ng ml−1) based on its inhibitory effect on the addition reaction between methyl green and sulfite ion is proposed. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of methyl green at 596 nm between 2 and 4 min using a fixed time method. Artificial neural networks with back propagation algorithm coupled with an orthogonal array design were applied to the modeling of the proposed kinetic system and optimization of experimental conditions. An orthogonal design was utilized to design the experimental protocol, in which pH, concentration of sulfite, temperature, and concentration of methyl green were varied simultaneously. Optimum experimental conditions in term of sensitivity were generated by using ANNs. The rate of decrease in absorbance is inversely proportional to the concentration of Hg(II) over entire concentration range tested (100-550 ng ml−1) with a detection limit of 45 ng ml−1 and a relative standard deviation at 200-400 ng ml−1 Hg(II) of 3.2% (n=5). A simple preconcentration step improved the limit of detection and linear dynamic range of the method to about 8 and 12-760 ng ml−1, respectively, by about 10 times enrichment of mercury between 12 and 75 ng ml−1. The method was based on enrichment of Hg(II) from dilute samples on an anionic ion exchanger fixed on a plastic strip and was applied to the determination of Hg(II) in environmental samples with satisfactory results.  相似文献   

9.
This paper presents two easy and selective methods for determining the active principles nafronyl (NFL) and naproxen (NAP), using a flow-through fluorescence optosensor based on the on-line immobilization on a nonionic-exchanger (Silica Gel, Davisil™ and Amberlite XAD 7, respectively) solid support. The determination was performed in 5×10−3 M HAc/NaAc buffer solution at pH 5 for NFL and 15×10−3 M glycine/HCl buffer solution at pH 2.5 for NAP at a working temperature of 20 °C. The fluorescence intensities were measured at λex/em=294/336 nm and λex/em=332/354 nm for NFL and NAP, respectively. The response time for these optosensors were practically instant, obtaining a linear concentration range between 0 and 700.0 ng ml−1 with a detection limit of 20.8 ng ml−1, an analytical sensitivity of 10.1 ng ml−1 and a standard deviation of 1.27% at a 500 ng ml−1 concentration level for NFL and a linear concentration range between 0 and 200.0 ng ml−1 with the detection limit of 13.3 ng ml−1, an analytical sensitivity of 6.0 ng ml−1 and a standard deviation of 3.52% at a 100 ng ml−1 concentration level for NAP. The proposed methods were satisfactorily applied to real samples (three commercial formulations and urine samples). The effects of the possible interferences were evaluated in all cases.  相似文献   

10.
This study aimed at developing competitive enzyme-linked immunosorbent assays (ELISAs) for the organophosphorus (OP) insecticide fenitrothion using a monoclonal antibody. The hapten used to obtain the antibody had an ideal structural feature that allowed minimal functional group sacrifice. By using the antibody and a coating antigen, a competitive indirect ELISA was developed, which showed an IC50 of 14 ng mL−1 with a detection limit of 3.0 ng mL−1. A competitive direct ELISA using an enzyme tracer was also developed, which showed an IC50 of 17 ng mL−1 with a detection limit of 1.6 ng mL−1. The antibodies in both assays showed negligible cross-reactivity with the metabolites of fenitrothion and other OP pesticides except with the insecticides parathion-methyl and parathion-ethyl. Recoveries of fenitrothion from fortified rice and lettuce samples were determined and the bias in the recovery values was rationalized by using the standard curves obtained in the matrix extract.  相似文献   

11.
Tso-Ying Chou 《Talanta》2009,80(2):493-9119
Partitioned dispersive liquid-liquid microextraction (PDLLME), using THF as the dispersive solvent and dichloromethane as the extraction solvent, was utilized to isolate and concentrate phenylurea herbicides (PUHs) from aqueous samples. In PDLLME, a dispersive solvent should be able to partition in the organic extractant droplets to effectively extract the polar organic compounds from aqueous samples. The mixture of the water-immiscible extractant and the partitioned dispersive solvent was obtained by centrifugation, dried under low pressure, reconstituted in methanol-water mixture (1:1), and injected into a HPLC system for the determination of PUHs. The enrichment factors of the PUHs ranged from 68 to 126 under the optimal conditions. The linear range was 0.5-100 ng ml−1 for each analyte, the relative standard deviations of PUHs were in the range of 1.5-5.9% (n = 5), and the detection limits (signal-to-noise ratio of 3) ranged from 0.10 to 0.28 ng ml−1 for the herbicides. The range of intraday precision (n = 5) for PUHs at the levels of 0.5, 5, and 50 ng ml−1 were 3.0-5.9%, 1.8-3.3%, and 2.2-3.6%, respectively. The range of interday precision (n = 5) at 0.5, 5, and 50 ng ml−1 were 0.4-1.8%, 1.2-2.4%, and 0.9-2.3%, respectively. The recoveries of PUHs from three spiked river water samples, at a level of 10 ng ml−1, were 91.2-104.1%. Due to its rapidity, ease of operation, and high recovery, PDLLME can be utilized to isolate and concentrate organic environmental contaminants such as PUHs from aqueous samples.  相似文献   

12.
A new method based on near-infrared (near-IR) fluorescence recovery, employing a two-reagent system which is composed of an anionic heptamethylene cyanine (HMC) and a polycationic phthalocyanine dye, Alcian blue 8GX, is presented for the determination of nucleic acids. With a maximum excitation wavelength at 766 nm and a maximum emission wavelength at 796 nm, the fluorescence recovery is linear with the concentration of nucleic acids added. Factors including the acidity of the medium, the reaction time, the optimal ratio of the two reagents, as well as the influence of foreign substance were all investigated. Meanwhile, the mechanism of fluorescence recovery was also studied. Under the optimal conditions, the linear ranges of the calibration curves were 10-250 ng ml−1 for calf thymus DNA (CT DNA) and 10-200 ng ml−1 for yeast RNA. The detection limits were 6.8 ng ml−1 for CT DNA and 6.3 ng ml−1 for yeast RNA, respectively. The method has been applied to the analysis of practical samples and the recovery results were satisfactory.  相似文献   

13.
The incorporation of melamine into food products is banned but its misuse has been widely reported in both animal feeds and food. The development of a rapid screening immunoassay for monitoring of the substance is an urgent requirement. Two haptens of melamine were synthesized by introducing spacer arms of different lengths and structures on the triazine ring of the analyte molecular structure. 6-Aminocaproic acid and 3-mercaptopropionic acid were reacted with 2-chloro-4,6-diamino-1,3,5-triazine (CAAT) to produce hapten 1 [3-(4,6-diamino-1,6-dihydro-1,3,5-triazin-2-ylamino) hexanoic acid] and hapten 2 [3-(4,6-diamino-1,6-dihydro-1,3,5-triazin-2-ylthio) propanoic acid], respectively. The molecular structures of the two haptens were identified by 1H nuclear magnetic resonance spectrometry, mass spectrometry and infrared spectrometry. An immunogen was prepared by coupling hapten 1 to bovine serum albumin (BSA). Two plate coating antigens were prepared by coupling both haptens to egg ovalbumin (OVA). A competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed to evaluate homogeneous and heterogeneous assay formats. The results showed that polyclonal antibodies with high titers were obtained, and the heterogeneous immunoassay format demonstrated a better performance with an IC50 of 70.6 ng mL−1, a LOD of 2.6 ng mL−1 and a LOQ of 7.6 ng mL−1. Except for cyromazine, no obvious cross-reactivity to common compounds was found. The data showed that the hapten synthesis was successful and the resultant antisera could be used in an immunoassay for the rapid and sensitive detection of this banned chemical.  相似文献   

14.
Wu X  Chen X  Hu Z 《Talanta》2003,59(1):115-121
A high-performance liquid chromatographic (HPLC) method is described for the simultaneous determination of honokiol and magnolol in rat plasma. The plasma was deproteinized with acetonitrile which contained an internal standard (diphenyl) and was separated from the aqueous layer by adding sodium chloride. Honokiol and magnolol are extracted into the acetonitrile layer with high yield, and determined by reversed-phase HPLC and ultraviolet detection. The limits of quantitation for honokiol and magnolol were 13 and 25 ng ml−1 in plasma, respectively, and recovery of both analytes was greater than 93%. The assay was linear from 20 to 200 ng ml−1 for honokiol and from 40 to 400 ng ml−1 for magnolol. Variation over the range of the standard curve was less than 15%. The method was used to determine the concentration-time profiles of honokiol and magnolol in the plasma following rectal administration of Houpo extract at a dose of 245 mg kg−1, equivalent to 13.5, 24.4 mg kg−1 of honokiol and magnolol, respectively.  相似文献   

15.
Masson JF  Obando L  Beaudoin S  Booksh K 《Talanta》2004,62(5):865-870
A sensor to detect markers of cardiac muscle cell death at less than 3 ng ml−1 and in less than 10 min has been achieved. This fiber-optic-based surface plasmon resonance (SPR) sensor is being applied to detect myoglobin (MG) and cardiac troponin I (cTnI) in HEPES buffered saline solution. An in vivo sensor for the early detection of the onset of myocardial infarction (MI) will greatly enhance the patient care. MG and cTnI are two biological markers released from dying cardiac muscle cells during an MI, and their detection at biologically-relevant levels can be diagnostic of MI. Antibodies specific to an antigen of interest are attached to a carboxymethylated dextran layer on a gold SPR surface. With the method developed, the lower limit of detection (LOD) for MG is 2.9 ng ml−1 at 25 °C. The biological level for MG reaches 15-30 ng ml−1 in patient blood after myocardial damage. A Langmuir adsorption isotherm describes the binding well. For cTnI, a lower detection limit of 1.4 ng ml−1 was achieved in preliminary tests. cTnI levels are in the range of 1-3 ng ml−1 in patient blood after myocardial damage. The antibody reaction with the carboxymethylated dextran surface was optimized by modifying the reaction pH, the temperature, and the dextran chain length.  相似文献   

16.
《Analytica chimica acta》2003,491(1):47-56
We simultaneously determined carbendazim, fuberidazole and thiabendazole by excitation-emission matrix (EEM) fluorescence in combination with parallel factor analysis (PARAFAC). Three-way deconvolution provided the pure analyte spectra from which we estimated the selectivity and sensitivity of the pesticides, and the relative concentration in the mixtures from which we established a linear calibration. Special attention was given calculating such figures of merit as precision, sensitivity and limit of detection (LOD), derived from the univariate calibration curve. The method, which had a relative precision of around 2-3% for the three pesticides, provided limits of detection of 20 ng ml−1 for carbendazim, 4.7 ng ml−1 for thiabendazole and 0.15 ng ml−1 for fuberidazole. The accuracy of the method, evaluated through the root mean square error of prediction (RMSEP), was 27.5, 1.4, and 0.03 ng ml−1, respectively, for each of the pesticides.  相似文献   

17.
A liposome immune lysis assay for enrofloxacin in carp and chicken muscle   总被引:1,自引:0,他引:1  
A homogenous complement-mediated liposome immune lysis assay (LILA) was developed for the determination of enrofloxacin (ENRO) in carp and chicken muscle. ENRO was covalently coupled to DPPE, and then immobilized onto the surface of liposomes by reverse-phase evaporation method. The performed liposome would be specifically lysed by the sequential additions of anti-ENRO monoclonal antibody (MAb) and guinea pig complement. Through a competitive assay format, the performed liposome can be used to detect ENRO in a range of 5.0-20 ng mL−1 in assay buffer. The limit of detection of ENRO in carp and chicken muscle was 1 ng g−1 and the limit of quantification was 2 ng g−1. Recoveries ranged from 58.3% to 65.2% for carp and 55.6-63.8% for chicken muscle at spiked levels of 2-8 ng g−1, with intra-assay and inter-assay variations 5.6-12.3% and 7.1-19.2%, respectively.  相似文献   

18.
Sun Y  Tang Y  Yao H  Zheng X 《Talanta》2004,64(1):156-159
A sensitive flow injection chemiluminescence (FL-CL) method for the determination of cephalosporin antibiotics, was developed. The method was based on that cephalosporin antibiotics could enhance the CL reaction of glyoxal and KMnO4 in sulfuric acid. Method development included the optimization of reagent concentrations and flow-rate. Under the optimized conditions, three cephalosporin antibiotics: cefalexin, cefadroxil, and cefazolin sodium, were determined. The detection limits of the method are 10 ng ml−1 cefalexin, 2 ng ml−1 cefadroxil, and 2 ng ml−1 cefazolin sodium. The method was successfully applied to the determination of three cephalosporin antibiotics in pharmaceutical preparations.  相似文献   

19.
An amperometric immunosensor for the specific and simple detection of 3,4-methylenedioxyamphetamine (MDA) and its analogues, 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyethylamphetamine (MDEA) in saliva and urine was developed. A direct competitive assay in which free analyte and horseradish peroxidase labelled species were simultaneously added to an immobilised polyclonal antibody was employed. Both MDA and MDMA could be labelled with the enzyme and the use of an MDMA-HRP tracer greatly enhanced the sensitivity of the assay. Amperometric detection was performed at +100 mV versus Ag/AgCl, using tetramethylbenzidine (TMB)/H2O2 as substrate. The antibody, raised specifically against the methylenedioxy moiety of an MDA-BSA immunogen allowed highly specific detection of these analogues with negligible cross-reactivity towards any other amphetamine related compounds. Total assay time was 45 min and the standard curve using MDA could be evaluated within the range 0.61-400 ng ml−1 with corresponding limit of detection (LOD) of 0.36 and 0.042 ng ml−1 for saliva and urine, respectively. The cross-reactivity pattern of the analytes was determined and showed the order of sensitivity increased with increased alkyl chain length (MDA < MDMA < MDEA). The overall performance of the sensor, working range, precision and sensitivity demonstrate its usefulness for rapid and direct measurement of methylenedioxy analogues of ecstasy in saliva and urine. The sensor has better specificity than any previous method for ecstasy, with greater sensitivity than ELISA methods, is less expensive/assay with an “easier to use” format than previous methods. The detection works in saliva or urine, eliminating requirement of blood sampling, with improved precision.  相似文献   

20.
A sensitive procedure has been developed for selenium and tellurium determination in milk by hydride generation atomic fluorescence spectrometry (HG-AFS) after microwave-assisted sample digestion. The method provides sensitivity values of 1591 and 997 fluorescence units ng−1 ml−1 with detection limits of 0.005 and 0.015 ng ml−1 for Se and Te, respectively. The application of the developed methodology to the analysis of cow milk samples of the Spanish market evidenced the presence of concentration ranges from 11.1 to 26.0 ng ml−1 for Se, and from 1.04 to 9.7 ng ml−1 for Te having found a good comparability with data obtained after dry-ashing of samples.  相似文献   

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