免疫吸附剂吸附类风湿因子（RF）

以聚乙烯醇水凝胶为载体，氨基酸为选择性配基，制备亲合性免疫吸附剂，用于清除类风湿性关节炎患者体内的类风湿因子（ＲＦ），实验中对水凝胶载体交联度、选择性配基种类、手臂效应等与吸附及选择性能的关系进行了研究。实验数据表明：当水凝胶交联度为４０％时，联接Ｌ－丝氨酸对ＲＦ具有最好的吸附选择性能。对患者血浆中ＲＦ－ＩｇＭ、ＲＦ－ＩｇＧ、ＲＦ－ＩＧＡ的吸附率分别达到６２．８％、４７．２％、和６０．０％，而对血浆蛋白无吸附。     

镁离子型Birnessite的合成和表征

采用ＭｎＯ－４和Ｍｎ２＋在强碱性条件下制得层状结构的Ｍｇ＿Ｂｉｒｎｅｓｓｉｔｅ，用ＸＲＤ（Ｘ射线多晶粉末衍射）、ＦＴ－ＩＲ（傅里叶红外光谱）、ＴＧ－ＤＴＧ／ＤＴＡ（热失重－微商热失重／热分析）等方法对它进行了表征。Ｍｇ＿Ｂｉｒｎｅｓｉｔｅ在２００～４００℃时为无定形，在６００℃生成结晶良好的Ｍｎ２Ｏ３相。ＣＣｌ４、ＣＨ３ＯＨ、ｎ＿Ｃ６Ｈ１４的吸附等温线类似于第三种类型的吸附等温线，在接近饱和的情况下都有凝聚现象产生。在６００℃，比表面和吸附数据表明出现层状结构塌陷。吸附吡啶ＦＴ－ＩＲ谱表明在Ｍｇ＿Ｂｉｒｎｅｓｉｔｅ中存在非质子酸（Ｌ酸）和质子酸（Ｂ酸）酸位。     

水热法合成钛硅酸盐新型无机离子交换剂

用水热法合成出一种新型的结晶水合钛硅酸盐（ＣＳＴ）,并用ＸＲＤ、ＳＥＭ、ＤＴＡ－ＴＧＡ、Ｘ－荧光方法对它进行了表征。在１ｍｏｌ／Ｌ ＨＮＯ３的水溶液中,ＣＳＴ对Ｃｓ交换量为０．６３ｍｍｏｌ／ｇ,在ｐＨ＝３时,ＣＳＴ对Ｃｓ的交换量达最大为１．６ｍｍｏｌ／ｇ,表明ＣＳＴ可以在酸性水溶液中作为Ｃｓ的离子交换剂使用。     

ASSESSMENT OF CRITICAL AGGREGATE CONCENTRATION （CAgC） BY UV． A NEW METHOEOLOGY

ＡＳＳＥＳＳＭＥＮＴ　ＯＦ　ＣＲＩＴＩＣＡＬ　ＡＧＧＲＥＧＡＴＥ　ＣＯＮＣＥＮＴＲＡＴＩＯＮ　（ＣＡｇＣ）　ＢＹ　ＵＶ．　Ａ　ＮＥＷ　ＭＥＴＨＯＥＯＬＯＧＹＡＳＳＥＳＳＭＥＮＴＯＦＣＲＩＴＩＣＡＬＡＧＧＲＥＧＡＴＥＣＯＮＣＥＮＴＲＡＴＩＯＮ（ＣＡｇＣ...     

壳聚糖吸附剂的制备及其性能

本文以壳聚糖为主要原料，分别经过悬浮交联和复合制备得到壳聚糖（Ｃｈｉｔｏｓａｎ）树脂吸附剂（简称ＣＳ吸附剂）和壳聚糖—活性炭（Ｃｈｉｔｏｓａｎ－Ａｃｔｉｖｅｃａｒｂｏｎ）复合吸附剂（简称ＣＳ—ＡＣ吸附剂）。研究影响制备吸附剂的诸因素；测定两种吸附剂的性能；探讨两种吸附剂对印染废水的脱色和对有毒金属离子的去除。研究结果表明：本研究条件下制得的ＣＳ和ＣＳ—ＡＣ吸附剂比表面积分别为８２．９Ｍ２／ｇ和９３７．３Ｍ２／ｇ；对亚甲基蓝溶液（５００ｍｇ／Ｌ）的静态吸附量为１４１．２ｍｇ／ｇ吸附剂和８３５．１ｍｇ／ｇ吸附剂；ＣＳ吸附剂对印染废水的动态脱色量为１２６．９ｍｇ／ｇ吸附剂；两种吸附剂对有毒重金属Ｐｂ（２＋）的去除率均达９０％以上，而且耐酸、碱性能较好，均为优良的颗粒状吸附剂。     

吸附型“人工肝”辅助材料的制备及其性能研究：Ⅱ.免疫吸附剂的制？…

本文介绍以醋酸乙烯酯为单体,二乙烯苯为交联剂制得大孔共聚物,经皂化,活化后,偶联ＩｇＧ制得免疫吸附剂、讨论了不同活化剂对偶联配体的影响,观察其对人血清中的ＨＢｓＡｇ的吸附性能。     

Cl_q—VT免疫吸附剂制备及其吸附循环免疫复合物性能初探

本文在最佳条件下将猪Ｃｌｑ与活化的ＶＴ树脂反应制备了Ｃｌｑ－ＶＴ免疫吸附剂，每克干重ＶＴ可结合０．５ｍｇＣｌｑ，可吸附１．４ｍｇ热凝聚人ＩｇＧ（ＡＨＧ）。ＳＤＳ—ＰＡＧＥ结果表明Ｃｌｑ－ＶＴ免疫吸附剂只对ＡＨＧ特鼻吸附，而不吸附其它血清蛋白。应用Ｃｌｑ－ＶＴ免疫吸附剂吸附ＳＬＥ病人血浆中循环免疫复合物（ＣＩＣ），结果表明约有５２％的ＣＩＣ被清除。Ｃｌｑ－ＶＴ免疫吸附剂只能一次性地使用。     

丁基罗丹明B.四溴荧光素双荧光剂萃取荧光法测定镓

１引言本文在选择性、灵敏度较高的丁基罗丹明Ｂ（ＢＲＢ）甲苯萃取荧光法测定镓的基础上，调ｐＨ值使ＧａＣｌ４ＢＲＢ三元络合物解析，ＢＲＢ进入水相。加入与ＢＲＢ颜色相近的四溴荧光素（ＴＢＦ），形成的ＢＲＢ２·ＴＢＦ再被甲苯萃取，萃取液中加入等体积正丁醇后进行测定。由于离子对（ＢＲＢ２·ＴＢＦ）中双荧光剂的叠加效应，使方法具有较高的灵敏度，检出限由原方法的０．３μｇ／Ｌ降为０．１μｇ／Ｌ，用于矿样中镓的测定，结果满意。２实验部分２．１仪器与试剂岛津ＲＦ－５４０荧光分光光度计；ｐＨＳ－３Ｃ型精密ｐＨ计（上…     

K2CO3活化煤矸石制备活性炭吸附剂

用Ｋ２ＣＯ３化学活化煤矸石制备适用于废水处理的活性炭吸附剂．考察了活化条件对产物的比表面、孔体积及灰分的影响．增加了前处理和后处理步骤以改善产物的性能．最佳条件下获得的活性炭吸附剂ＢＥＴ比表面达１２３６ｍ２／ｇ，孔体积０．６７９ｃｍ３／ｇ．所制得的吸附剂表面是疏水性的，对水溶液中的酚类污染物有良好的吸附性能．     

CHO-EPO-EGFR细胞株的构建及在光固定EGF材料上的生长

采用人工细胞（ｌｉｐｏｓｏｍｅ）法将两种质粒ｐＣＥＰＯ及ｐＲＣ／ＣＭＶ／ＥＧＦＲ转染宿主细胞－中国仓鼠卵巢细胞（ＣＨＯ）,得到表达水平稳定,形态良好的细胞株ＣＨＯ－ＥＰＯ－ＥＧＦＲ,研究该细胞株在光固定ＥＧＦ的聚苯乙烯材料上生长情况。     

Characterization of the Fc receptors of the murine leukemia L1210

A glycoprotein extract prepared from the plasma membranes of L1210 cells was passed over columns of Sepharose 4B to which either heat-aggregated human IgG or F(ab')2 fragments has been coupled. The intact IgG column bound 35.7 percent of the applied counts, whereas the F(ab')2 columns bound 2.8 percent. The bound glycoproteins were eluted with citrate buffer (pH 3.2) and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Three peaks with apparent molecular weights of 65,000 45,000, and 28,000 daltons were identified and purified by electroelution from polyacrylamide gels. The isolated proteins were able to bind to the same sub-classes of mouse IgG myeloma proteins as the intact L1210 cells, indicating that these molecules are related to L1210 surface Fc receptors. Amino acid analyses of the 3 proteins were markedly similar suggesting that the observed molecular heterogeneity might be due to carbohydrate differences. Neuraminidase digestion of the isolated proteins resulted in mobility shifts on polyacrylamide gel electrophoresis which were consistent with the interpretation that either the isolated proteins have considerably different sialic acid contents, or that removal of the sialic acid results in disaggregation of an Fc receptor molecule.     

In‐Tandem Column Arrangement for Rapid Purification of IgG using a Blue Avid Gel P and an Aza‐Arenophilic Affinity Gel

Abstract

An in‐tandem study comprising two affinity chromatographic columns, Blue Avidgel P (for the selective removal of albumin) and an aza‐arenophilic gel (Gel A) containing a nitrogen capping nucleophile (for the selective removal of IgG), was conducted to obtain high purity IgG from serum. The results demonstrated that highly pure IgG (>97%) can be obtained from the phenethylamine capped aza‐arenophilic gel column after the serum was passed through the Blue Avidgel P column in contrast to the 38% IgG purity obtained when the serum was passed through a Gel A column alone under similar conditions. Further studies show that IgG selectivity is not dependent on the nature of the support material used in the synthesis of these aza‐arenophilic gels since the synthesis of aza‐arenophilic gels using either Sepharose or Fractogel yield similar results.     

变色硅胶吸附脱除模拟柴油中各种碱性氮化物

研究了变色硅胶吸附脱除氮含量为960.56μg/g模拟柴油中的碱性氮化物喹啉、苯胺和吡啶。比较了氧化铝、硅藻土、硅胶及变色硅胶对模拟柴油中喹啉的吸附脱除效果。采用XRD、低温N_2吸附-脱附和NH_3-TPD等方法对硅胶和变色硅胶进行了表征。考察了粒径、吸附温度、吸附时间、剂油质量比及共存芳香化合物(萘、苯或甲苯)对变色硅胶吸附脱除各种碱性氮化物的影响。变色硅胶吸附脱除碱性氮化物的顺序均为苯胺吡啶喹啉。吸附时间对三种氮化物的吸附脱除没有影响;吸附温度、变色硅胶粒径和共存芳香化合物对苯胺和吡啶的吸附脱除效果影响不大,对喹啉的吸附脱除效果影响较为明显;剂油质量比对三种氮化物的吸附脱除影响均较大,尤其是对喹啉影响最大。结果表明,变色硅胶吸附各种氮化物时Co能够与其中的N原子形成配位络合吸附。经焙烧再生,变色硅胶几乎完全恢复了对喹啉和吡啶的吸附脱除能力,并可多次再生,但变色硅胶再生后对苯胺的吸附能力损失较大。     

Thermal degradation of cereal straws in air and nitrogen

Multigram quantities (2.5-10 g) of highly purified IgG were obtained within 4 h from serum by using Avid AL packed in a radial-flow column. Avid AL is an affinity gel containing a synthetic, low-mol-wt ligand capable of selectively binding IgG from serum of all animal species tested. By packing the gel in a radial-flow column up to 500 mL, a high flow rate of 50 mL/min can be achieved without adversely affecting the performance of the gel and the purity of the isolated antibody.     

Depletion of high-abundance proteins from human plasma using a combination of an affinity and pseudo-affinity column

Human serum albumin (HSA) and immunoglobulin G (IgG) represent over 75% of all proteins present in human plasma. These high-abundance proteins prevent the detection of low-abundance proteins which are potential markers for various diseases. The depletion of HSA and IgG is therefore essential for further proteome analysis. In this paper we describe the optimization of conditions for selective depletion of HSA and IgG using affinity and pseudo-affinity chromatography. A BIA Separations CIM (convective interaction media) Protein G disk was applied for the removal of IgG and the Mimetic Blue SA A6XL stationary phase for the removal of HSA. The binding and the elution buffer for CIM Protein G disk were chosen on the basis of the peak shape. The dynamic binding capacity was determined. It was shown to be dependent on the buffer system used and independent of the flow rate and of the concentration of IgG. Beside the binding capacity for the IgG standard, the binding capacity was also determined for IgG in human plasma. The Mimetic Blue SA A6XL column was characterized using human plasma. The selectivity of the depletion was dependent on the amount of human plasma that was loaded on the column. After the conditions on both supports had been optimized, the Mimetic Blue SA A6XL stationary phase was combined with the CIM Protein G disk in order to simultaneously deplete samples of human plasma. A centrifuge spin column that enables the removal of IgG and HSA from 20 μL of human plasma was designed. The results of the depletion were examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis.     

IgG配基对肿瘤坏死因子α的吸附机制研究

以球形琼脂凝胶为载体,键连免疫球蛋白IgG配基,通过化学修饰等方法,研究了IgG与TNFα的亲和作用机制,进而推测出吸附作用位点.结果显示,IgG分子的氨基、羧基及色氨酸残基中的吲哚基可能影响吸附作用;IgG配基连接手臂可以提高吸附性能,在3000U/mLTNFα血浆中,采用己二胺手臂吸附量可达7084.68U/mL.鉴于IgG配基的高效特异性,研究结果不仅具有一定的理论意义,也有较强的临床应用前景     

Protein A tangential flow affinity membrane cartridge for extracorporeal immunoadsorption therapy.

Tangential flow affinity membrane cartridge (TFAMC) is a new model of immunoadsorption therapy for hemoperfusion. Recombinant Protein A was immobilized on the membrane cartridge through Schiff base formation for extracorporeal IgG and immune complex removal from blood. Flow characteristics, immunoadsorption capacity and biocompatibility of protein A TFAMC were studied. The results showed that the pressure drop increased with the increasing flow rate of water, plasma and blood, demonstrating reliable strength of membrane at high flow rate. The adsorption capacities of protein A TFAMC for IgG from human plasma and blood were measured. The cartridge with 139 mg protein A immobilized on the matrix (6 mg protein A/g dry matrix) adsorbed 553 mg IgG (23.8 mg IgG/g dry matrix) from human plasma and 499.4 mg IgG (21.5 mg IgG/g dry matrix) from human blood, respectively. The circulation time had a major influence on IgG adsorption capacity, but the flow rate had little influence. Experiments in vitro and in vivo confirmed that protein A TFAMC mainly adsorbed IgG and little of other plasma proteins, and that blood cell damage was negligible. The extracorporeal circulation system is safe and reliable.     

CPS 768 Formation of anisotropic ice-agar composites by directional freezing

Aqueous agar gels were directionally frozen in a temperature gradient of 10 °C/ cm at rates between 8.8×10^–5 cm/sec and 8.8×10^–4 cm/sec to form highly anisotropic ice-agar composites. Observations of the vitrified agar domains which were localized at the ice grain boundaries were made and the ice domain size was measured as a function of the solidifying conditions. The segregation of the noncrystalline agar was dominated by the vitrification character of the agar gel. Thermal analysis indicated that ice crystallization rate decreased to a negligible level as the water content of the gel decreased to approximately 50 percent by weight agar.     

Syntheses of ordered mesoporous silica by new hybrid template

Ordered mesoporous silica with macroscopic shape has been prepared with a hybrid template of gel and poly(ethylene oxide)₁₀₆–poly(propylene oxide)₇₀–poly(ethylene oxide)₁₀₆ (pluronic F127) surfactant, where both water-soluble agar gel and pluronic F127 significantly affect the mesoporous structure and morphology of silica. The thermal analysis revealed the noticeable interaction between agar and F127, which contributes to the formation of homogenous hybrid template. In the hybrid template, agar gel contributed to the maintenance of morphology structure, while F127 was responsible for the formation of ordered porous structure in silica solids.     

采用一锅法制备量子点/琼脂纳米复合凝胶

以天然高分子琼脂为稳定剂,采用简单便捷的一锅法制备Mn掺杂ZnS量子点/琼脂纳米复合凝胶,琼脂不仅作为制备量子点的稳定剂,同时也是纳米复合凝胶的主要成分。对该纳米复合凝胶中量子点的化学结构和尺寸大小进行了表征,并对纳米复合凝胶的荧光性能和凝胶性能进行了研究。实验结果表明,制备得到的纳米复合凝胶均一稳定,在302 nm紫外光下呈现十分明显的橙红色荧光。在该纳米复合凝胶的透射电子显微镜（TEM）表征中可以观察到大小比较均一、粒径为3 nm左右的纳米粒子,光谱分析结果进一步证实纳米复合凝胶中存在Mn掺杂ZnS量子点。该纳米复合凝胶不仅具有良好的荧光性能,还具有温度刺激响应性可逆溶胶-凝胶转变性能,同时具有较高的溶胶转变温度和较好的温度稳定性。利用这些性能特点,可以方便地制备纳米复合凝胶小球。此外,该纳米复合凝胶还可以被潜在应用于金属离子的荧光检测分析领域。