A novel ratiometric sensor for the fast detection of palladium species with large red-shift and high resolution both in aqueous solution and solid state

A highly selective fluorescent probe (OHBT) was designed and synthesized by linking the ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-(hydroxyphenyl) benzamide) (HBTBC) to the palladium specificity response group, allyl group, for the detection of palladium species in aqueous solution. The allyl group can be hydrolyzed by Pd⁰ species through the Pd⁰-catalyzed Tsuji–Trost reaction and thus release the fluorophore HBTBC, which shows two emission bands. The maximum emission spectra originated from the enol and keto forms at 415 and 555 nm respectively and with no overlap, which implies the high resolution of the palladium detection. The palladium species can also be detected by paper strip because of the solid-state fluorescence of probe HOBT catalyzed by palladium. This method was successfully applied in the palladium related Suzuki–Miyaura coupling reaction and the detection limit is lower than 1 μM.     

A polythiophene-derived ratiometric fluorescent sensor for highly sensitive determination of carbenicillin in aqueous solution

A new ratiometric fluorescent sensor based on a quaternized quinine substituted 3-phenylpolythiophene derivative (PTQ1) was designed and developed for facile and reliable detection of carbenicillin with high sensitivity and selectivity.     

Benzimidazolium-based new simple ratiometric fluorescent sensor for selective detection of dihydrogenphosphate

A series of pyridine-coupled benzimidazolium-based receptors 1, 2 and 3 have been designed and synthesised. In the series, only receptor 1 is structurally appealing in the selective recognition of H₂PO₄^?  in CHCl₃ as well as in CH₃CN over a series of other anions. The ratiometric change in emission with a triplet band at 420 nm is the distinctive feature of selective recognition of H₂PO₄^?  in CHCl₃. In CH₃CN, a ‘turn on’ response is selectively observed. Binding studies have been carried out using fluorescence, UV–vis, ¹H NMR and ³¹P NMR spectroscopic techniques. Experimental results have been correlated with the theoretical findings.     

A TCF-based colorimetric and fluorescent probe for palladium detection in an aqueous solution

A new fluorescent probe (TCF-AC) that contains 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) skeleton has been developed. Probe TCF-AC exhibits highly selective and sensitive detection toward Pd⁰ in EtOH/H₂O (1:1, v/v, PBS 20?mM, pH?=?7.4) solution with fluorescence “turn on” and colorimetric changes. The Pd⁰ detection by TCF-AC holds some advantages including good anti-interference ability, a relative large Stokes shift (>100?nm), and a low detection limit (7.05?×?10^?7?M). Cell imaging studies demonstrate that TCF-AC is applicable to detect Pd⁰ in living HeLa cells.     

A sensitive colorimetric and ratiometric fluorescent probe for mercury species in aqueous solution and living cells

A highly sensitive and selective fluorescent probe for inorganic and organic mercury species displays colorimetric and ratiometric response in a buffer solution via mercury promoted cleavage reaction. The probe is demonstrated to detect CH(3)HgCl in living cells.     

Turn-on ratiometric fluorescent sensor for Pb detection

We report a ratiometric lead fluorescent sensor (LFS-1) with high affinity to Pb²⁺ that shows considerable selectivity over 12 other physiological related metal cations in aqueous media. Binding induces excimer formation, providing a highly sensitive ratiometric measure of lead concentrations.     

A new magnetically recoverable heterogeneous palladium catalyst for phosphonation reactions in aqueous micellar solution

A new heterogeneous palladium complex of 2‐aminothiophenol supported on nanomagnetic γ‐Fe₂O₃ was synthesized and characterized using various methods. The catalyst was used as a magnetically recoverable heterogeneous palladium catalyst for phosphonation reactions via C ? P bond formation. Using this method, a wide range of electrophilic benzenes was coupled successfully with phosphite esters (triethyl/tri‐isopropyl/triphenylphosphite and diethyl/di‐isopropyl/diphenylphosphite) in aqueous micellar solution to generate the corresponding arylphosphonates in good to high yields. The catalyst was separated using an external magnet and reused for six consecutive cycles without any significant loss of its reactivity. Copyright © 2015 John Wiley & Sons, Ltd.     

Pyrenoviologen-based fluorescent sensor for detection of picric acid in aqueous solution

Two highly emissive pyrenoviologen derivatives were synthesized and used to fabricate fluorescent sensors for detection of picric acid (PA) with good sensitivity and selectivity.     

Pyrenoviologen-based fluorescent sensor for detection of picric acid in aqueous solution

Two highly emissive pyrenoviologen derivatives were synthesized and used to fabricate fluorescent sensors for detection of picric acid(PA) with good sensitivity and selectivity.The sensitivity of the sensor was attributed to the specific electrostatic association effect of the cationic pyrenoviologens to the picrate anions,which also gave the sensor special selectivity among other compounds with similar structure.The electron transfer between them was attributed to the fluorescence response.Fluorescence lifetime measurements revealed that the quenching is static in nature.The novel and efficient pyrenoviologen derivatives-based sensors offered a strategy to fabricate real-life PA sensor.     

A new click reaction generated AIE-active polymer sensor for Hg2+ detection in aqueous solution

We described herein a new AIE-active polymer sensor incorporating triazole moiety for Hg²⁺ detection in aqueous solution. The polymer sensor P1 was synthesized from tetraphenylethene and diazidobenzene via click reaction. It shows typical AIE feature, and emits cyan fluorescence in the mixture of tetrahydrofuran and water, reaching the strongest fluorescence when the fraction of water (f_w) is 90%. In aqueous solution (f_w?=?90%), the polymer sensor can exhibit fluorescence quenching response towards Hg²⁺ over other competing metal ions, with the fluorescence color changed from cyan to almost no emission, which can be clearly observed by the naked eyes under 365?nm UV lamp.     

A near-infrared fluorescent sensor for detection of cyanide in aqueous solution and its application for bioimaging

A new NIR fluorescent sensor based on an amine-substituted heptamethine cyanine dye displayed a highly selective fluorescence enhancement with cyanide in aqueous solutions, and was applied for the imaging of anthropogenic and biogenic cyanide.     

A ratiometric fluorescence sensor for caffeine

The dye disodium 3,4:3',4'-bibenzo[b]thiophene-2,2'-disulfonate can be used as a molecular probe for the fluorimetric detection of caffeine in aqueous solution. The fluorescence response is attributed to non-covalent interactions of caffeine with the dye in the ground state and in the excited state. The bimodal interaction allows performing ratiometric measurements with very good selectivity over structurally related analytes. The dye was also used to develop a simple test strip for the visual differentiation of normal and decaffeinated coffee with a standard UV lamp.     

A colorimetric and ratiometric fluorescent probe for palladium

A colorimetric and ratiometric fluorescent probe for the palladium species has been developed based on the Pd(0)-catalyzed cleavage of an allyoxycarbonyl group of amines under mild conditions. The probe displays a highly sensitive and selective response with significant changes in both color (from colorless to jade-green) and fluorescence (from blue to green), through the ICT process.     

A turn on ESIPT probe for rapid and ratiometric fluorogenic detection of homocysteine and cysteine in water with live cell-imaging

5(Benzothiazol-2-yl)-4-hydroxyisophthalaldehyde (BHI), an intense ESIPT containing molecule in mixed media loses its properties due to resonance-assisted H-bond (RAHB) in absolute water. Due to resonance-assisted H-bond the o-aldehyde is more reactive than the other one. With addition of cysteine/homocysteine into this solution the o-aldehyde group gets transformed into thiazolidine/thiazine ring, respectively, and the phenolic proton becomes free enough for transfer to nitrogen of the benzothiazole ring in excited state, that is, the ESIPT of BHI is turned on. Thus we can detect cysteine/homocysteine in water as well as in live cells.     

Novel fluorescent sensor for detection of Cu(II) in aqueous solution

A novel fluorescent chemosensor based on aminonaphthol, which can selectively recognize copper(II) over other metal ions in aqueous solution within a broad pH span, was synthesized.     

Colorimetric sensor for ATP in aqueous solution

A new chromogenic complex 1.Zn has been synthesized, and its interactions with different biologically important phosphates have been investigated in aqueous solution (pH approximately 7.2). A visual color change can be detected on binding of ATP to 1.Zn, whereas no such change is observed when other biologically related anions (AMP, ADP, PPi, or Phosphate) are used. Complex 1.Zn can also be used as a staining agent for yeast cells allowing detection under normal light microscopy.     

A novel fluorescent sensor for hydrophobic amines in aqueous solution

A novel fluorescent tweezer was designed and synthesised to sense hydrophobic amines in aqueous solution. Association of the guest was driven by both hydrophobic effect and electrostatic interactions, with the hydrophobic interactions being dominant. The affinity and selectivity of the sensor for amine-based stimulants are reported.     

A highly selective and ratiometric fluorescence probe for the detection of Hg and pH change based on coumarin in aqueous solution

A simple but highly selective coumarin-based fluorescence probe 1, 8-(1,3-dithiane)-7-hydroxycoumarin was designed and synthesized for both the ratiometric detection of Hg²⁺ and the on–off response to pH change in aqueous solution. The sensor detected Hg²⁺ selectively via Hg²⁺-promoted thioacetal deprotection reaction within five minutes and reflected pH in the range from 7.8 to 11.9 as a result of the equilibrium between weak-fluorescent acid form and strong-fluorescent base form. In addition, the probe has an excellent selectivity towards Hg²⁺ over other competitive metal ions for biomedical and environmental applications. The sensing behavior of our probe was studied by UV–visible absorption spectra and fluorescence spectra.     

A new perylene diimide-based fluorescent chemosensor for selective detection of ATP in aqueous solution

A new perylene diimide (PDI) ligand (1) functionalized with a Zn²⁺–dipicolylethylenediamine (Zn²⁺–DPEN) moiety was synthesized and first used as a fluorometric chemosensor to specifically detect the presence of ATP over other phosphate anions in aqueous solution. The solution of 1 upon addition of ATP displayed a remarkable absorption decrease compared with other anions, indicating the selectivity of 1 to ATP. Moreover, among these anions investigated, only ATP showed a considerable enhancement of fluorescent intensity of 1. The fluorescence molar ratio plot combined with the ESI-TOF mass measurement determined that binding stoichiometry between 1 and ATP is 1:1.     

Developing a novel ratiometric fluorescent probe based on ESIPT for the detection of pH changes in living cells

As an important parameter of intracellular metabolism, pH plays important roles in maintaining normal physiological processes. The abnormal pH could cause disorder of cell function which may cause neurological diseases. Herein, we present two novel ratiometric fluorescent probes to detect pH changes. The probes employed 2-(2′-hydroxyphenyl)benzothiazole as fluorescent platform, and displayed desirable fluorescence response to pH on the basis of excited state intramolecular proton transfer (ESIPT) process. The probe BtyC-1 showed green fluorescence at 546 nm under acidic conditions, while it displayed strong blue fluorescence at 473 nm and weak green fluorescence at 546 nm under alkaline conditions. Biological experiments demonstrated that the probe BtyC-1 could be successfully applied for the ratiometric imaging of cellular pH and the NH₄Cl-induced pH changes in living cells.