Absorption spectra of photosensitized human fat tissue

We study experimentally the optical characteristics of adipose tissue in vitro in its dye sensitization—by indocyanin green and brilliant green. We conducted experiments on a PerkinElmer Lambda 950 spectrophotometer in the spectral range of 250–900 nm. From an analysis of the measured absorption spectra, we determine the shifts in the maximum of the absorption band for the studied photosensitizers in adipose tissue in comparison to their spectra in solutions. These shifts are explained by the bonding of photosensitizer molecules with the protein matrix of adipose tissue. We find the relative monomer and dimer concentrations of dye molecules in solutions and stained samples.     

浊点萃取-分光光度法测定彩笔中的灿烂绿

建立了浊点萃取-分光光度法测定灿烂绿的方法.以非离子型表面活性剂Triton X-114为萃取剂,富集、分离灿烂绿,采用分光光度法进行检测.研究了缓冲溶液用量、表面活性剂用量、平衡温度和平衡时间对浊点萃取的影响,得到最佳实验条件：5％的Triton X-114溶液2.5mL、pH3.8的盐酸-柠檬酸钠缓冲溶液2.0mL、平衡温度和时间分别为50℃和15min.在优化的实验条件下,灿烂绿被萃取到Triton X-114相与水相分离.该方法用于彩笔中灿烂绿的测定,结果满意.     

Ultrastructural study of the ovary of the sugarcane spittlebug Mahanarva fimbriolata (Hemiptera)

The present study describes the ultrastructure of meroistic telotrophic ovaries of the sugarcane spittlebug Mahanarva fimbriolata. In this type of ovary, nurse cells, oogonia, and prefollicular tissue are located at the terminal (distal) regions or tropharium of ovarioles. Oocytes in different developmental stages, classified from I to V, are observed in the vitellarium. Stage I oocytes do not exhibit intercellular spaces in the follicular epithelium, suggesting that synthesis and production of yolk during this stage occurs only through endogenous processes. Small yolk granules of different electron densities are present in the cytoplasm. Few lipid droplets are observed. Stage II oocytes exhibit small intercellular spaces in the follicular epithelium. More protein as well as lipid yolk granules are observed in the cytoplasm. In stage III oocytes, intercellular spaces in the follicular epithelium are larger than those observed in the previous stage. Electrondense protein granules of various sizes, larger than those observed in stage II oocytes predominate in the cytoplasm. Smaller lipid droplets are also present. In stage IV oocytes, the follicular epithelium exhibits large intercellular spaces. Our data clearly indicate that the opening of these spaces in the follicular epithelium of M. fimbriolata oocytes increases as the intake of exogenous proteins intensifies, that is, in stages IV and V oocytes. During these stages, granular yolk becomes viscous due to the lysis of granules. In stage V oocytes, viscous yolk predominates in the cytoplasm. This type of yolk, however, has not been described for other orders of insects. The chorion of M. fimbriolata oocytes consists of an external layer (exochorion) and an internal one (endochorion), which is in direct contact with the oocyte. Numerous small pores that probably facilitate oxygenation of the internal structures inside the eggs are observed in the exochorion.     

取代效应对脂滴检测NAPBr染料分子的双光子吸收和激发态性质影响的理论探究

双光子荧光染料分子在生物医学成像中具有广阔的应用前景,但取代效应对分子结构以及光物理性质影响的探求相对匮乏. 本文设计并研究了一系列脂滴检测染料分子,分析了分子的光学性质以及无辐射跃迁等. 通过分子内弱相互作用和电子- 空穴布居分析,阐述了其内在机理. 结果表明,所研究的分子均具有优良的光物理性能、高效荧光量子产量、大的斯托克斯位移以及显著的双光子吸收截面等. 本工作合理地解释了实验现象并阐述了取代效应对脂滴检测NAPBr染料分子的双光子吸收和激发态性质的影响,这为设计新型的高效有机分子提供了理论指导.     

Quantitative approach to ultrasonic emulsion separation

Ultrasound of 2MHz was irradiated to the emulsion prepared from canola oil and water and flocculation of the oil droplets occurred immediately. By putting the emulsion sample in a thin glass cell and setting it in bath type irradiation equipment, the progress of the separation was quantitatively monitored with the optical absorbance. The use of the cell enables visual observation of the behavior of oil droplets. Pictures show the formation of flocks of the dispersed phase and the appearance of checkered pattern consisting of flocks at a regular interval. The observation indicates that the action of radiation forces on oil droplets, which causes the flocculation. The flocks started to rise after stopping irradiation with holding their shape. The rising rate of the flocks was significantly greater than that of oil droplets in the original emulsion. Ultrasonic irradiation caused a rapid decrease in the absorbance, which expresses a progress of the separation. Effects of two major operation parameters, power and time on the separation degree were examined. The degree improved with increasing power input and irradiation time. The dataset was arranged in a plot of normalized separation degree against the input energy. The plot suggests that effective separation was attained with a lower power input and a longer irradiation time. The plot provides a guide for setting condition for the separation.     

偏振态对分布式光纤泄漏检测系统的影响研究

研制了一种基于Sagnac干涉仪的直线型分布式光纤传感器,可实时进行管道泄漏检测与定位。分析和研究了该干涉仪的泄漏检测原理、泄漏源定位方法以及光偏振态对传感器性能的影响。当两束光偏振态相同时,系统具有最佳的性能;当两束光正交时,无法实现信号检测。管道泄漏的实验结果表明,该系统能较准确地确定泄漏源位置,且定位误差为0.69%。     

Surface optical sensitization of a thin polycrystalline silver iodide film by brilliant green molecules

The effect of the surface optical sensitization (SOS) of silver iodide in a multilayer structure by trace amounts of gaseous molecules of brilliant green dye is demonstrated. Using the surface plasmon resonance excitation, it is shown that the SOS causes changes in the surface layer parameters and can be used to develop highly sensitive and selective gas sensors.     

Effect of decreasing glucose concentration in blood plasma after blood saturation with oxygen

It was experimentally detected that the glucose concentration in blood plasma decreases after venous blood saturation with oxygen (oxygenation process). This effect was recorded for several tens of donor blood samples using IME-DC (Germany) and Optium Omega (USA) portable glucometers, as well as an optical method using holographic sensors based on hydrogel polymer films, independent of the presence of oxygen in plasma. During blood oxygenation in the organism, glucose is redistributed between blood plasma and erythrocyte cytoplasm in favor of cytoplasm. The observed effect is explained by an increase in the electric field during blood oxygenation in lipids of the erythrocyte cytoplasmic membrane, which orients dipoles of asymmetric glucose molecules along the normal to the membrane surface. This results in erythrocytemembrane permeability asymmetry.     

Synergistic inhibition of malignant melanoma proliferation by melphalan combined with ultrasound and microbubbles

The cavitational effects of ultrasound (US) exposure induce transient pores on the cell membrane (sonoporation). Sonoporation have been applied in the field of cancer therapy by promoting delivery of extracellular molecules such as drugs and genes into cytoplasm. In addition, it is known that using US together with microbubbles (MB) elevates permeability of these agents. In this study, by applying the US-MB strategy for melanoma chemotherapy, we evaluated the antitumor effect of melphalan combined with US-MB on a melanoma cell line (C32) in vitro and in vivo. The in vitro cytotoxic effect of the melphalan with US-MB was greater than that of melphalan alone or melphalan in combination with US. In vivo experiments using xenografts, intratumoral injection of melphalan and MB with US exposure led to a greater degree of tumor regression than did the intratumoral injection of the melphalan alone or melphalan in combination with US. These results suggest that US-MB promotes the antitumor effect of melphalan by increasing delivery of molecules into cells and that this strategy may become an effective method of adjuvant therapy against malignant melanoma.     

Simulation of multilayer injection lasers with internal emission leaking

A mathematical model of multilayer injection lasers, based on the self-consistent kinetic problem taking into account the correlation between optical and electrical characteristics of a device is presented. The light-current characteristic of such laser is measured. A fast algorithm for computing complex eigenvalues of the wave equation is proposed. The calculation results are compared with experimentally measured light-current characteristics of diode lasers (fabricated by the GNOptics Company) with emission leaking to an optical cavity. The optical field profiles are determined in subthreshold and above-threshold modes for structures of high-power injection lasers with internal emission leaking. Good agreement between experimental and calculated data is achieved.     

Optical irradiation method for fiber coupler fabrications

An optical irradiation method for high-speed fiber coupler fabrications without contamination is proposed. In optical fiber taper fabrications we found an interesting effect that fiber elongation is self-arrested although laser irradiation is continued. This is a typical effect in the optical irradiation method, but is not seen in the flame method or the thermal heating method. The “self-controlled fiber taper shape effect” is theoretically explained by considering thermal energy flows, and optical fiber couplers are fabricated using this effect. From our experiments, we show that optical fiber coupler fabrications at high-speed without contamination are possible using the optical irradiation method.     

Live Cell Imaging With Biocompatible Fluorescent Carbon Quantum Dots Derived From Edible Mushrooms Agaricus bisporus,Pleurotus ostreatus,and Suillus luteus

In the study, fluorescent imaging of live cells was performed using fluorescent carbon quantum dots derived from edible mushrooms species; Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus as a fluorophore agent. Carbon quantum dots were synthesized through a facile and low-cost method based on microwave irradiation of dried mushroom samples in hydrogen peroxide solution under optimized conditions (microwave energy, solution type, duration of microwave treatment, amount of mushroom). Upon purification with centrifugation, microfiltration, and dialysis, the lyophilized carbon quantum dots were identified through UV–visible, fluorescence and FT-IR, X-ray photoelectron spectroscopy, X-ray diffraction, high-resolution transmission electron microscopy, and quantum yield calculation. Cell viability assessment of the carbon quantum dots was evaluated against human epithelial cell line PNT1A using the Alamar Blue Assay. In vitro fluorescence cell imaging studies demonstrated that the carbon dots could dynamically penetrate the cell membrane and nuclear membrane and localize in both the cytoplasm and the nucleus.

     

Direct Visualization of Vesicle Maturation and Plasma Membrane Protein Trafficking

Internalization and intracellular trafficking of membrane proteins are now recognized as essential mechanisms that contribute to a number of cellular processes. Current methods lack the ability to specifically label the plasma membrane of a live cell, follow internalization of labeled membrane molecules, and conclusively differentiate newly formed membrane-derived vesicles from pre-existing endocytic or secretory structures in the cytoplasm. Here, we detail a visualization method for surface biotinylation of plasma membrane-derived vesicles that allows us to follow their progress from membrane to cytosol at specific time points. Using the transmembrane receptor RET as a model, we demonstrate how this method can be applied to identify plasma membrane-derived vesicle maturation, determine RET’s presence within these structures, and monitor RET’s recycling to the cell surface. This method improves on static and less discriminatory methods, providing a tool for analysis of real-time vesicle trafficking that is applicable to many systems.     

Nanostructures and luminescence properties of porous ZnO thin films prepared by sol–gel process

ZnO thin films containing nano-sized pores were synthesized on solid substrates through a sol–gel process by accommodating cetyl-trimethyl-ammonium bromide (CTAB) as an organic template in the precursor solution. By X-ray diffraction the resultant ZnO films were found to possess ordered pore arrays forming lamellar structure with the spacing between two adjacent pores being ∼3.0 nm. Photoluminescence measurements indicated that the surfactants effectively passivated the surface defects of the ZnO films responsible for the green emission. Al doping was found to improve not only the lamellar structure of the pore arrays but also the near-band-gap emission intensity while the suppression effect of CTAB on the green emission remained undisturbed. With a proper control of doping level, the optical property as well as the structural integrity can be tailored to augment the potential of ZnO films for the optoelectronics and sensor applications.     

基于Sagnac光纤干涉仪的管道泄漏检测和定位技术

研制了一种基于Sagnac干涉仪的分布式光纤传感器,可实时进行管道泄漏检测与定位.阐述了该传感技术的工作原理和泄漏点定位方法,并推导了泄漏引起的光信号相位变化表达式。管道泄漏实验结果表明,水压为0.3MPa时,该技术能有效地检测到管道泄漏的发生并准确定位。     

Active measuring method for investigating the irradiation effect on the gain characteristics of an erbium-doped fiber amplifier in inter-satellite optical communication

An active measuring method is proposed for the first time to study the irradiation effect on the gain characteristics of an erbium-doped fiber amplifier (EDFA) for inter-satellite optical communication, which is feasible from the engineering viewpoint. The measuring method based on the irradiation model can predict the irradiation effect on the gain characteristics just by measuring the variation of the EDFA signal output power at one irradiation dose rate in the irradiation experiment. Therefore, one can avoid removing the erbium-doped fiber (EDF) from the EDFA, which is needed in the previously tested passive measuring method. These experimental measurements meet completely the requirements of the technical specifications for inter-satellite optical communication. Finally, an irradiation experiment has also been carried out to evaluate the new method by a simple test procedure.     

Mechanical resonance properties of porous graphene membrane; simulation study and proof of concept experiment

Mechanical resonance properties of porous graphene resonators were investigated by simulation studies. The finite element method was utilized to design the porous graphene membrane pattern and to calculate the mechanical resonance frequency and quality factor. The changes in the resonance frequency and quality factor were systematically studied by changing the size, number, and relative location of pores on the graphene membrane. Mass loss and carbon-carbon bond break were found to be the main competing parameters for determining its mechanical resonance properties. The correlation between the geometry and the damping effect on the mechanical resonance of graphene was considered by suggesting a model on the damping factor and by calculating the membrane deflections according to the pore location. Based on the simulation results, an optimal porosity and porous geometry were found that gives the maximum resonance frequency and quality factor. Suspended graphene with various number pore structures was experimentally realized, and their mechanical resonance behaviors were measured. The trend of changes in resonance frequency and quality factor according to the number of pores in the experiment was qualitatively agreed with simulation results.     

双波长双指示剂催化分光光度法测定水和茶叶中的痕量锰

研究在pH 5.6的HAc-NaAc缓冲溶液中,氨三乙酸活化痕量锰离子催化高碘酸钾氧化灿烂绿和萘酚绿B的指示反应,通过测定460nm和630nm下催化体系与非催化体系吸光度值的变化,建立了双波长双指示剂催化分光光度法测定痕量锰的新方法。在最佳实验条件下,测定痕量锰离子的线性范围为0.010—0.60μg/25mL。检出限为1.2×10-10g/mL。此法用于水和茶叶中痕量锰的测定,结果满意。     

Periodic poling of optical waveguides produced by swift-heavy-ion irradiation in LiNbO<Subscript>3</Subscript>

The generation of periodically poled structures in waveguides prepared by swift-heavy-ion (SHI) irradiation, i.e. in the electronic stopping power regime, has been achieved following two different strategies. In one of them we have prepared bulk PPLN samples by an applied electrical field, followed by irradiation with F ions at 22 MeV. After the ion irradiation, a waveguide showing a high optical confinement is obtained, preserving the original PPLN structure. The second strategy consisted of electric periodic poling of previously fabricated swift-ion-irradiated waveguides. To our knowledge this method has not been, so far, successful for conventional implanted waveguides. The successful fabrication of PPLN structures on novel waveguides prepared by SHI irradiation offers a promising potential for nonlinear integrated optical devices.     

脉冲微波辐照影响心肌细胞膜蛋白构象及其机制的研究

应用显微傅里叶变换红外光谱技术研究了脉冲微波辐照对心肌细胞膜蛋白质构象、功能的影响和相关分子机制。结果表明,辐照可对心肌细胞的细胞膜蛋白质结构产生明显影响。细胞膜脂质中—CH₂—、磷脂结构中CO、蛋白质酰胺Ⅰ,Ⅱ带的伸缩振动峰消失或位移。辐照后心肌细胞膜蛋白质二级结构也出现明显变化,α-螺旋和β-折叠结构减少,二级结构无序化程度增加。上述变化均与辐照剂量呈正相关。结果提示受脉冲微波辐照后,心肌细胞膜蛋白构象的完整性受损,膜稳定性及流动性下降,膜上多种生物活性结构被破坏,上述变化构成了细胞膜功能丧失、细胞形态和结构损伤、细胞凋亡等病理学效应的生物化学基础。文章首次从蛋白质构象角度阐述了微波辐照对心肌细胞膜损伤的分子病理机制。