共查询到20条相似文献,搜索用时 15 毫秒
1.
The use of electrospray Ionization (ESI) tandem mass spectrometry (MS/MS) for the structural determination of the lipid A components of the psychrophilic bacterium Pseudoalteromonas haloplanktis TAC 125 is reported. The lipid A contains the classical bisphosphorylated beta-(1' --> 6)-linked D-glucosamine disaccharide with 3-hydroxydodecanoyl residues (12 : 0 (3-OH)) linked both as esters and amides to 2', 3' (distal glucosamine) and 2, 3 positions (proximal glucosamine) of the sugar backbone. The hydroxyl of 12 : 0 (3-OH) fatty acid linked at the 3' position is esterified by a dodecanoyl residue (12 : 0). In addition to the pentaacyl component, a minor tetraacyl lipid A, lacking the acyl residue at position 3, was also found in the lipid A fraction. The advantage of this MS technique for the investigation of the intra-ring fragmentation, which is useful for the determination of fatty acyl residue distribution on each glucosamine unit, is emphasized. 相似文献
2.
Bernadete Pereira da Silva Patricia Oliveira Campos Jose Paz Parente 《Chemistry of Natural Compounds》2006,42(3):316-321
A new bisdesmosidic furostanol saponin, along with a known spirostanol saponin, furcreastatin, were isolated from Furcraea gigantea Vent. (Agavaceae). The structure of the new saponin was elucidated as 3-[(O-6-deoxy-α-L-mannopyranosyl-(1→4)-O-β-D-glucopyranosyl-(1å 3)-O-[O-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranosyl)oxy]-(3β, 5α, 25R)-26-(β-D-glucopyranosyloxy)-22-hydroxyfurost-12-one. The structural identification was performed using a combination of spectroscopic techniques and chemical conversions. Furcreastatin showed a powerful haemolytic effect in the in vitro assay, but the new bisdesmosidic furostanol saponin demonstrated only a significant inhibition of the capillary permeability activity. 相似文献
3.
De Castro C Carannante A Lanzetta R Lindner B Nunziata R Parrilli M Holst O 《Chemistry (Weinheim an der Bergstrasse, Germany)》2006,12(17):4668-4674
Three different oligosaccharide structures from the lipooligosaccharide fraction of Agrobacterium tumefaciens strain A1 were determined by means of chemical and spectrometrical methods. The peculiar feature of this oligosaccharide family consisted of its unusual length, that was very close to the that minimal requested for the external membrane functionality as exemplified from oligosaccharide 3, where the inner core is glycosylated from only one sugar moiety onwards. 相似文献
4.
Huanhuan Cui Yingxing Wang Shenghui Li Changhu Xue 《Journal of carbohydrate chemistry》2013,32(5-6):398-411
AbstractA galactoglucan (HCPA) was extracted from C. mollissima Blume fruits using hot water, which was composed of glucose and galactose, with a molecular weight of 5.5?kDa. Methylation and 1?D, 2?D NMR spectroscopy analysis showed that HCPA contained [→4)-α-D-Glcp(1→], [→4,6)-α-D-Glcp(1→], [→4)-β-D-Galp(1→] and [α-D-Glcp(1→] motifs in a molar ratio of approximately 6.11:1.08:1.00:1.03. HCPA showed a spherical-like structure as detected by scanning electron microscope (SEM). HCPA biological activity was investigated in vitro. The results showed that HCPA inhibited the proliferation of HeLa, MCF-7 and MGC-803 cells, induced HeLa cells apoptosis and arrested cell cycle in G2/M phase of HeLa cells. HCPA also enhanced the viability and phagocytic ability of RAW 264.7 cells and stimulated the production of NO, TNF-α and IL-6. The results demonstrated that HCPA had a potent antitumor and immunomodulating effects in vitro, suggesting its potential use as functional food and drug products. 相似文献
5.
酵母甘露聚糖硫酸酯化前后结构与生物活性比较研究 总被引:3,自引:2,他引:3
对酵母甘露聚糖及硫酸酯化酵酯母甘露聚糖的相对分子质量、红外光谱、离子色谱、比旋光度、IO4^-氧化、SO4^2-含量、Smith降解、电泳及THP-1细胞分泌IL-la的水平等进行了测定,结果表明,硫酸酯化后相对分子质量增加,IR光谱[α]D^20均有明显变化,电泳迁移加快,IO4^-氧化甲酸生成量减少,硫酸酯化位置在非还原末端,C(3)对碱位置稳定,Smith降解结果表明硫酸化前后基本结构无明显变化。硫酸酯化前后样品对THP-1细胞分泌IL-1a水平影响差异显,对IL-6,IL-8,TNFa,IL-2亦有影响。 相似文献
6.
Magorzata Szczesio Katarzyna Gobis Izabela Korona-Gowniak Ida Mazerant-Politowicz Dagmara Ziembicka Henryk Foks Marek Gwka Andrzej Olczak 《Acta Crystallographica. Section C, Structural Chemistry》2020,76(7):673-680
Four new picolinohydrazonamide derivatives, namely, 6‐methyl‐N′‐(morpholine‐4‐carbonothioyl)picolinohydrazonamide, C12H17N5OS, 6‐chloro‐N′‐(morpholine‐4‐carbonothioyl)picolinohydrazonamide methanol monosolvate, C11H14ClN5OS·CH3OH, 6‐chloro‐N′‐(4‐phenylpiperazine‐1‐carbonothioyl)picolinohydrazonamide, C17H19ClN6S, and 6‐chloropicolinohydrazonamide, C6H7ClN4, have been synthesized and characterized by NMR spectroscopy and single‐crystal low‐temperature X‐ray diffraction. In addition, their antibacterial and anti‐yeast activities have been determined. The first three compounds adopt the zwitterionic form in the crystal structure regardless of the presence or absence of solvent molecules in the structure. They also adopt the same symmetry, i.e. P21/c (P21/n), unlike the fourth structure which is chiral and has the space group P212121. For all the studied cases, intermolecular N—H…O and N—H…N hydrogen bonds play an essential role in the formation of the structures. 相似文献
7.
8.
9.
Dr. Malika Makhlouf Brahmi Dr. Cyril Portmann Danilo D'Ambrosio Dr. Tom M. Woods Damiano Banfi Patrick Reichenbach Laeticia Da Silva Emilie Baudat Dr. Gerardo Turcatti Prof. Dr. Joachim Lingner Prof. Dr. Karl Gademann 《Chemistry (Weinheim an der Bergstrasse, Germany)》2013,19(14):4596-4601
By using the Telospot assay, 27 different extracts of cyanobacteria were evaluated for telomerase inhibition. All extracts showed varying, but significant activity. We selected Microcystis aeruguinosa PCC 7806 to identify the active compound and a bioassay guided fractionation led us to isolate mixtures of sulfoquinovosyl diacylglycerols (SQDGs), which were identified by 2D NMR and MS/MS experiments. Pure SQDG derivatives were then synthesized. The IC50 values of pure synthetic sulfoquinovosyl dipalmitoylglycerol and the monopalmitoylated derivative against telomerase were determined to be 17 and 40 μM , respectively. A structure–activity relationship study allowed the identification of compounds with modified lipophilic acyl groups that display improved activity. 相似文献
10.
11.
12.
R. Yu. Milusheva N. L. Voropaeva I. N. Ruban G. V. Nikonovich S. Sh. Rashidova 《Chemistry of Natural Compounds》2000,36(2):120-123
Structural features of chitin from Aral crustaceans and chitosan based on it are examined using x-ray diffraction and IR spectroscopy. Their biological activities on encapsulated cotton seeds are determined.Institute of Polymer Chemistry and Physics, Academy of Sciences of the Republic of Uzbekistan, Tashkent, 700128, ul. A. Kodirii, 76, fax 144 26 61. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 97–99, March–April, 2000. 相似文献
13.
14.
15.
Yuxing Tan Zhijian Zhang Yuhuan Tan Daizhi Kuang Jiangxi Yu Xiaoming Zhu 《Journal of Coordination Chemistry》2017,70(15):2606-2624
Three new dialkytin complexes, {[o-OH–C6H4(O)C=N–N=C(CH2Ph)COO](n-Bu2Sn)}n (1), {[o-OH–C6H4(O)C=N–N=C(CH2Ph)COO](MeOH)(p-MeC6H5CH2)2Sn}2 (2), and {[o-OH–C6H4(O)C=N–N=C(CH2Ph)COO](EtOH)(C6H5CH2)2Sn}2 (3), were synthesized by reactions of 2-oxo-3-phenylpropionic acid salicyloylhydrazone with the corresponding diorganotin(IV) complex, respectively. All the complexes were characterized by IR, 1H, 13C, 119Sn NMR spectra, elemental analysis, X-ray single crystal diffraction and TGA. For in vitro antitumor activities, complexes were evaluated by the MTT assay against three human cancer cell lines (NCI-H460, HepG2 and MCF7) and human cell line (HL7702). The results showed that 1 may be a better potential candidate for further chemical optimization and cancer therapy than 2 and 3. The interactions between the complexes and calf thymus DNA were studied; the interaction of 1 with calf thymus DNA was intercalation, 2 and 3 were intercalation and electrostatic binding. 相似文献
16.
磺酰胺类化合物是继磺酰脲与咪唑啉酮除草剂之后开发的乙酰乳酸合成酶 (ALS)抑制剂的另一重要领域 ,已筛选出一些高活性的新品种[1~ 3] 。为寻找新的性能优良的活性物质 ,进一步研究活性与结构的关系 ,本文设计将磺酰胺类和酰胺类除草剂的基本骨架用一个亚甲基 CH2 连结起来 ,相当于在磺酰脲桥链中间插入了一个亚甲基 ,共合成了 1 6个新的邻烷氧基羰基苯磺酰胺衍生物( 2a~ 2p) ,并初步测试了它们的生物活性。1 实验部分1 1 仪器和试剂JEOLFX 90Q型、BRUKERAC P2 0 0型核磁共振仪 ,TMS为内标 ;Shimad… 相似文献
17.
18.
19.
Tris-, di-(organotitanium) substituted tungstosilicates α、β-[(CpTi)3(SiW9O37)]7- and γ-[(Cp-Ti)2(SiW10O38)]6- were prepared by the reaction of Cp2TiCl2 (Cp = η5-C5H5) with α、β-SiW9O349- (noted α、β-SiW9), γ-SiWO368- (noted γ-SiW10) . The products were characterized by means of elemental analysis, IR, 1H NMR, 183W NMR and polarography. 183W NMR spectra of the complexes support the stoichiometry of the new heteropolyanions and the probable retention of the A-XWg or γ-SiW10 units. And the organotitanium substituted compounds showed promising in vitro antitumor activity in two of human tumor cell lines. 相似文献
20.
K. S. Zhauynbaeva M. Kh. Malikova D. A. Rakhimov Z. A. Khushbaktova 《Chemistry of Natural Compounds》2003,39(6):520-522
A homogeneous native acetylated glucomannan of molecular weight 32000 was isolated from bulbs of N. poeticus. A linear chain polymer consisting of -14-bound D-manno- and D-glucopyranoses was found using periodate and chromic oxidation and methylation. The glucomannan has low toxicity and exhibits distinct hypolipidemic activity. 相似文献