共查询到20条相似文献,搜索用时 93 毫秒
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Superox系列固定相是性能优良的氢键型固定相,适用地脂肪醇分析,非级性固定相OV-1柱,经减活处理后亦适宜醇类异构体分离,考察了C1-C2异构醇的36个组分在分离效能。OV-1柱和Superox0.6柱上的保留行为,柱效和极限温度,结果表明,两种柱对醇都有很好的分离效能。OV-1柱柱效及柱温极限高,除了戊醇-3和戊醇-2是重叠峰外,其余组分间有一定分离度,其分离顺序基本上是根据组分的沸点,包括 相似文献
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研究了非晶硅膜改性的弹性玻璃毛细管交联OV-1701中等极性固定相色谱柱。在适当温度下,采用过氧化二异丙苯(DCUP)游离基引发交联OV-1701固定液,成功地制备了交联OV-1701柱,该柱具有柱效高、惰性好、耐溶剂、抗腐蚀和耐高温等性能,是一种新型高性能的中等极性交联柱。 相似文献
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采用温和条件下的溶胶-凝胶技术,成功制备了阴离子交换-反相混合模式硅胶基质毛细管电色谱整体柱。通过调整反应液中不同前体的比例,优化了整体柱的制备条件。通过扫瞄电镜,对柱床进行了表征和分析。实验发现,所制备的整体柱电渗流的方向和大小可随流动相pH值的改变而改变,在酸性和中性条件下,具有从阴极流向阳极的电渗流;当流动相pH值升至约7.5时,电渗流方向发生了反转(由阳极流向阴极)。在优化的实验条件下,用所制备的整体柱对所考察的酸性(中性)化合物实现了快速分离,并获得了高达160,000N/m的柱效。 相似文献
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建立了亲水相互作用色谱柱(HILIC)串联C18柱的方法,高效分离实际样品中的胭脂红,并对色谱柱的串联顺序以及色谱分离条件进行优化。结果表明:当HILIC柱在前C18柱在后,流动相为乙腈-乙酸铵(5 mmol/L)=91∶9(V/V),流速为0.9 mL/min,柱温为25℃,检测波长为508 nm时,此时胭脂红的分离效果最佳。在最佳的色谱分离条件下,选用两种市售饮品进行实际样品的测定,回收率在81.2%~119%。方法适用于水相样品中的强极性物质的高效分离与分析。 相似文献
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电色谱整体柱是通过原位聚合或固化于柱管内部的方法来制备的一种新型色谱柱.与常规的填充毛细管柱不同的是,其制备方法具有简易性和易于实现色谱填料表面化学性质多样性的特点,已迅速成为优异的毛细管电色谱固定相形式.本文综述了毛细管电色谱整体柱研究的最新进展. 相似文献
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程序涂渍柱“柱效能程序效应”的研究 总被引:1,自引:0,他引:1
本文从理论上给出程序柱“柱效能程序效应”的概念并导出了函数关系式,通过计算机可计算柱效能程序效应与柱内各变量之间的关系,实践证明实验数据的变化趋势与理论数据相符。 相似文献
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《Analytical letters》2012,45(9):595-602
Abstract The influence of column tubular material on column efficiency in high speed liquid chromatography has been examined. For good reproducibility in HETP, the columns were first packed dry with Corasil I (28–37μ) and then heptane saturated with 3,3′-oxydipropionitrile was flown through the column. When steady state conditions were achieved, the % loading of stationary phase was 1.1%. It was found that column materials of precision bore and seamless stainless steel, aluminum, and copper gave equivalent efficiency results for retained and unretained components. Teflon coated aluminum columns were not reproducible, in regard to HETP. 相似文献
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以甲基硅油OV-101、210为固定液,研究了出口压减压下薄涂气相色谱 柱的特性,从柱压、柱温、保留值、柱效、柱的稳定性等方面进行了探讨。应用于烷烃及几种农药的分离,效果良好。 相似文献
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对稀匀浆法填充半制备柱(Ф10 mm×100 mm)的过程进行研究,并用正交法对柱填充条件进行了优化,考察了压力、匀浆液组成、匀浆液体积对半制备柱填充效果的影响.由柱效和分离度定义式中的参数入手,讨论填充条件对柱性能的影响.通过计算总孔隙率ε_t、内部孔隙率ε_p、外部孔隙率ε_e、Van-Deemter曲线、吸附等温线等参数,发现填充压力对填充密度的作用最大,是最重要的影响因素;匀浆配比和匀浆体积共同影响其填充的均匀性.在压力11 MPa,流速275 mL/min,120 mL纯乙醇为匀浆液的条件下得到了最好的填充效果. 相似文献
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Guohong-Zhao Xiaoqiang-Lei Zhonglai-Wang Chengke-Gong Hanqing-Wang Liren-Chen 《Chromatographia》2003,58(7-8):465-469
Summary A new method is described to prepare polymeric porous-layer open-tubular (PLOT) columns by using a two-step in-situ polymerization technology. The integrated method involves a straightforward in-situ polymerization of the monomer. By using -(trimethoxysilyl)propyl methacrylate as a bridge, the porous polymer is bonded to the columns inner wall, and this polymer is crosslinked so the whole polymer looks like a single moleculer. The new column avoids the defects of traditional polymer PLOT columns where particles are easily released and swept through the column, causing blockage or a spiking detection signal. The new type of PLOT column is coated with a divinylbenzene and ethylene glycol dimethyl acrylate copolymer and has an increased polarity when compared to a conventional polymer Q-type PLOT column. The retention characteristics of the new column were evaluated and found to be comparable with the commercially available HP PLOT-U column. The inertness of the porous polymer allows the elution of a range of apolar and polar compounds: even most active and polar compounds such as H2O and H2S eluted symmetrically. The new column possesses high stability and can withstand temperatures up to 210 °C. With this new type of capillary columns, significantly better mechanical stability, temperature endurance, reproducibility, strong separation power and good inertness are obtained in combination with short analysis times. 相似文献