超临界流体色谱法分离生育酚同系物

用超临界流体色谱法进行了生育酚异构体的分离研究。在实验温度303．15K～343．15K和压力14～22MPa范围内，考察了ODS柱上温度、压力和密度与容量因子和选择性的关系，得到了生育酚在超临界色谱中的最佳分离条件：温度为343．15K，压力为20MPa，CO2流量750mL/min。     

黄酮类化合物的超临界流体色谱分离

利用超临界流体色谱成功地分离了黄酮类化合物，研究了流动相组成，柱条件，压力及温度的影响。发现流动相组成是影响色谱分离的最主要因素；其次，色谱柱条件也是影响分离的一个很重要的因素，硅胶基质的键合苯基柱比较适合于极性黄酮类化合物的分离。     

超临界流体色谱法分离手性化合物的进展

超临界流体色谱（ＳＦＣ）是一种很有潜力的色谱分离技术,可以弥补高效液相色谱（ＨＰＬＣ）和气相色谱（ＧＣ）在手性对蚋物分离方面的不足。本文不但介绍了ＳＦＣ仪器的结构特点,ＳＦＣ与手性固定相（ＣＳＰｓ）结合在手性分离领域中的应用和最新进展,还对ＳＦＣ与其它色谱技术的分离效果进行了评价。文献３９篇。     

超临界流体色谱分析番茄红素

以超临界CO2作为流动相，在压力15.0-20.0MPa，温度25－50℃，携带剂乙醇或正己烷的浓度分别为0－30％和0－20％的范围内考察了番茄红素及其氧化产物在C18色谱柱上的保留值的变化规律，确定了最佳的分离条件。对超临界丙烷萃取的番茄红素原料、萃取产物及萃余物进行了定量分析，考察了重复性及平行性。结果表明：在优化条件下，番茄红素的保留时间在3min以内，定量结果的重复性与平行性好。     

用超临界二氧化碳重结晶分离菲蒽混合物

建立了一套以超临界二氧化碳及其改性流体为重结晶溶剂的等压温差超临界流 重结晶实验装置。用色谱保留值法评价了ＳＦＲ分离菲蒽的试验条件。研究了该实验装置影响重结晶的因素，并对同分异构体型菲蒽模型混合物进行了ＳＦＲ分离纯化。     

超临界流体色谱在金属络合物和金属有机化合物中的分析应用

超临界流体色谱（SFC）在色谱分离过程中能在较低的温度下分析对热不稳定性的化合物,包括金属络合物和金属有机化合物。本文总结了近来文献报道的各种过渡金属、重金属、镧系和锕系以及铅、汞和锡的金属有机化合物的SFC分离,还讨论了SFC检测系统和金属有机化合物的溶解度的测定。     

Carbon Dioxide Supercritical Fluid Extraction with on-line Fluorescence Detection

Abstract

An HPLC detector was modified to operate as a low pressure on-line supercritical fluid extraction detector. Spectra of CO₂ and fluorene are presented of pressures ranging from 85 to 150 atm. Both the intensity and emission wavelength of fluorene were effected by CO₂ pressure. As pressure increased, the emission intensity also increased and the emission shifted to a shorter wavelength. The intensity of CO₂ emission decreased by increasing pressure. The emission wavelength of CO₂ was unaffected by pressure. A detection limit for fluorene was found to be 5 μg per extraction. The minimal detectable mass was 25 μg (± 8% RSD) per extraction.     

超临界流体色谱在生物工程中应用的新进展

超临界流体色谱（SFC）是高效液相色谱（HPLC）和气相色谱（GC）的重要补充技术。用SFC可以分离多数不能用GC分离的低挥发性物质。与传统的HPLC相比，SFC的分离速度更快、效率更高。本文综述了SFC在生物分子分离分析方面应用的新进展。引用文献74篇。     

超临界流体色谱保留行为的研究——烃类在非极性柱上的保留值

本文在 Martire 等人以平均场晶格模型处理超临界流体色谱(SFC)保留值方法的基础上,提出修正函数的假设,结合实验数据确定了修正函数的具体表达式为△′=(0.01605+0.02387n_■)ρ~3_■/T,-(0.01382+0.02055n_■)ρ~4_■/T_■。经过修正后的保留值方程可在较大密度范围内(ρ_■:0.705～1.632)定量描述正构烷烃在非极性柱上,超临界流体 CO_2中的保留值变化规律。     

超临界流体色谱法分离手性药物

对超临界流体色谱技术（SFC）在手性药物分离方面的研究与应用状况做了评述,对超临界流体色谱手性分离的分离方式、操作条件及影响因素作了介绍。     

超临界流体色谱的研究进展

超临界流体色谱作为气相色谱和液相色谱的有力补充可用于热不稳定和低挥发性物质的分析分离和制备,也可用于超临界流体中分子间相互作用的研究。本文从色谱的流动相、固定相、检测系统及应用几方面综述了超临界流体色谱近年来的研究进展。     

超临界流体萃取-胶束电动毛细管色谱法分离测定大黄中蒽醌类组分的含量

采用ＳＴＥ－ＭＥＣＣ法分离测定大黄中的芦荟大黄素，大黄素和大黄酸。萃取条件为压 力４１．４ＭＰａ，温度５０℃，改性剂量０．４ｍＬ。被测组分在１０ｍｉｎ得到全部分离。本法准确、简 便、快速、结果令人满意。     

Trapping Efficiencies of Various Adsorbents in Supercritical Fluid Extraction with Modified Carbon Dioxide

One way of collecting extracted analytes after a supercritical fluid extraction (SFE) is to pass the depressurized fluid through a trap composed of a solid adsorbent. The influence of the adsorbent nature on the trap efficiency has been studied. First, the retention factors of each compound of a polarity test mix (tetracosane, naphthalene, fluoranthene, acetophenone, N,N-dimethylaniline, 2-naphthol, decanoic acid) have been determined on five high specific area (greater than 800 m²/g) polymeric phases by supercritical fluid chromatography (SFC). The comparison of this values with those obtained on octadecyl silica (ODS) showed that polymeric phases have the greatest retention power. After that, the efficiency of a solid trap filled with the greatest retention power polymeric adsorbent was evaluated by using pure carbon dioxide and 2.5%, 5%, 10%, and 20% methanol-modified carbon dioxide. As expected, this trap permitted a quantitative collection of all the former compounds even when a content as high as 10% of methanol was implemented. A solid trap filled with ODS adsorbent allowed quantitative collection of all the compounds only at a methanol content lower than 2.5%.     

Physicochemical Principles and Applications of Supercritical Fluid Chromatography (SFC). New analytical methods (19)

In supercritical fluid chromatography (SFC) compressed gases in the region of their critical temperature are used as mobile phases. SFC has important advantages over gas chromatography (GC) for the separation of low-volatile or thermally unstable substances. Like high pressure liquid chromatography (HPLC) and gel chromatography, it is used for various special applications and preparative separations, e.g. in the petroleum industry and in the separation of oligomers. SFC is of great interest in fundamental research on fluid extraction and for the determination of the physicochemical properties of fluid systems. In this contribution the most important physicochemical, methodological, and instrumental principles of SFC are summarized; characteristic physicochemical applications are the determination of capacity ratios, partition coefficients, partial molar volumes, interaction second virial coefficients, and difusion coefficients.     

Separation of Chlorophylls and Their Degradation Products using Packed Column Supercritical Fluid Chromatography (SFC)

Complex mixtures of chlorophyll degradation products may arise during processing and storage of vegetable oil and green plant materials like broccoli and spinach. Determination of these compounds is important in the area of food chemistry. Therefore a method using packed column supercritical fluid chromatography (SFC) has been developed. The method comprises chromatography using a simple gradient of methanol in carbon dioxide at constant column back pressure of 30 MPa and a column temperature of 40°C. Effects of pressure and mobile phase composition showed the importance of applying a modifier gradient for optimal separation of the chlorophyll products. The method permits separation of 15 chlorophyll derivatives including chlorophyll a and b, pheophytins, and pyropheophytins on a C₁₈ column in about 20 minutes. Identifications of the individual peaks were based on reference compounds, the retention order of the compounds, and their absorption spectra.     

超临界二氧化碳微乳液中的表面活性剂

介绍了在超临界二氧化碳(SC-CO_2)下形成微乳液的原理和概念,并且按照所应用各种表面活性剂的结构特点对其在SC-CO_2下形成微乳液的能力分别进行比较和归纳,为今后筛选和设计在SC-CO_2下形成微乳液的表面活性剂提供了重要信息。     

超临界流体技术制备超细粒子

超临界流体用于制备超细粒子是一项新技术，本文综述了两种形成微粒的方法；超临界快速膨胀法和超临界反溶剂法，并着重介绍了其在研究和应用方面的进展。     

超临界流体萃取和高效液相色谱法测定银杏酸

介绍了一种用改性的超临界二氧化碳萃取和离线的反相高效液相色谱分析银杏叶提取物中银杏酸含量的方法。对萃取条件进行了优化,结果表明以硅胶为载体,在３０ＭＰａ、５５℃条件下,８％甲醇作改性剂时的超临界二氧化碳对银杏酸有最理想的回收率和选择性。最小检测限为０．４２ｍｇ／Ｌ。