STRUCTURAL STUDIES OF A FROM THE POLLEN OF PAPAVER SOMNIFERUM

ＳＴＲＵＣＴＵＲＡＬＳＴＵＤＩＥＳＯＦＡＦＲＯＭＴＨＥＰＯＬＬＥＮＯＦＰＡＰＡＶＥＲＳＯＭＮＩＦＥＲＵＭ￥ＰｉｎｇＭＩＡＯ；ＳｏｎｇＧＡＯａｎｄＳｈｅｎｇＪＩＮ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔｒｙ，ＰｅｋｉｎｇＵｕｉｖｅｒｓｉｔｙ，Ｂｅｉｊｉ...     

THE STUDY ON THE WATER SOLUBLE PEPTIDES IN PAPAVER SOMNIFERUM POLLEN EXTRACTION,PURIFICATION,SEQUENCE DETERMINATION AND SYNTHESIS

Four peptides PSPP1,PSPP2,PSPP3 and PSPP4 were purified from the water-extractof Papaver somniferum pollen.Their sequences,with 21,17,13 and 16 amino acid residuesrespectively,have been determined by Edman degradation-N-terminal dansylation.PSPP2,PSPP3 and PSPP4 were synthesized using solid phase method.The immunopromotiveactivities of PSPP1,PSPP2,PSPP3,PSPP4 and the initially separated sample PSPP havebeen also observed by the methods of counting erythrocyte rosette forming cells(ERFC)and T-lymphocyte transformation test in vitro.     

DESIGN AND SYNTHESIS OF ANALOGS OF THE SCHISTOSOMA MANSONI P28 ANTIGENIC PEPTIDEP141

ＤＥＳＩＧＮ ＡＮＤ ＳＹＮＴＨＥＳＩＳ ＯＦ ＡＮＡＬＯＧＳ ＯＦ ＴＨＥ ＳＣＨＩＳＴＯＳＯＭＡ ＭＡＮＳＯＮＩ Ｐ２８ ＡＮＴＩＧＥＮＩＣ ＰＥＰＴＩＤＥＰ１４１￥ＪｉａＸｉＸＵ；ＺｈｉＨｕｉＱＩＮ；ＪｕｎＹ．ＡＮＧ；ＭｅｎｇＳｈｅｎＣＡＩ（Ｓ...     

STRUCTURAL STUDIES OF AN ARABINOGALACTAN——A WATER SOLUBLE POLYSACCHARIDE FROM THE POLLEN OF PAPAVER SOMNIFERUM

A polysaccharide,PS(1),has been isolated from the pollen of papaver somniferum.PS(1)is ahighly branched arabinogalactan,which,mainly contains α—L—arabinofuranosyl(1→)and(3,6→1)—β—D—galactopyranosyl residues.We also studied the circular dichroism and immunoactivity of PS(1).     

SYNTHESIS AND DETERMINATION OF THE BIOLOGICAL ACTIVITIES OF YEAST ALANINE tRNA ANALOGUES

The role of base modification in yeast tRNAAl(?) function in protein synthesis was examined by the use of unmodified tRNA analogues. Unmodified full length tRNAs, 5'-half tRNAs (nucleotides 1-35) and 3'-half tRNAs (nucleotides 37-75) were transcribed in vitro using T7-RNA polymerase. Unmodified tRNA half molecules were joined to normally modified half molecules (5'-half, nucleotides 1-36; 3'-half, nucleotides 36-75) by T4-RNA ligase. Using this method, we synthesized three analogues of yeast tRNAAl(?): (i) tRNAAl(?) which lacks base modifications in the 5'-half of the molecule; (ii) tRNAAl(?) which lacks base modifications in the 3'-half of the molecule; and (iii) tRNAAla completely lacking base modifications. We determined the biological activities of these analogues. In rat aminoacyl-tRNA synthetase reactions, the alanine acceptance activity decreased by 52%, 79% and 85% when modified bases were absent from the 5'-half molecule, the 3'-half molecule or the total molecule, respectively. In rabbit retic     

THE SYNTHESIS OF 3'''',E''''-ALKYLENEDIOXY-ISOFLAVONE ANALOGS

ＴＨＥＳＹＮＴＨＥＳＩＳＯＦ３＇，Ｅ＇－ＡＬＫＹＬＥＮＥＤＩＯＸＹ－ＩＳＯＦＬＡＶＯＮＥＡＮＡＬＯＧＳ￥ＬｉｎｇＨｕａＣＡＯ（ＣｈｅｍｉｓｔｒｙＤｅｐａｒｔｍｅｎｔｏｆＸｉｎｊｉａｎｇＵｎｉｖｅｒｓｉｔｙ，Ｕｒｕｍｐｅ，８３００４６）Ａｂｓｔｒａｃｔ...     

THE SYNTHESIS OF UNSYMMETRIC ANALOGS OF GOSSYPOL—6-O-METHYLGOSSYPOL

6—O—Mcthylgossypol (3) was obtained with the methods of synthesis for the first time.For the purpose ofe一iminating some prob一ems assoeiatedwith the eliniea一use orgossypol such as irreversibility and hypokalemia,while retaining its pl飞armacologieally desirableantifertility effeets,numerous derivatives and analog     

SYNTHESIS OF N-GLUCURONIDES OF 5-FLUOROURACIL AND THEIR ANTITUMOR ACTIVITIES

ＳＹＮＴＨＥＳＩＳＯＦＮ－ＧＬＵＣＵＲＯＮＩＤＥＳＯＦ５－ＦＬＵＯＲＯＵＲＡＣＩＬＡＮＤＴＨＥＩＲＡＮＴＩＴＵＭＯＲＡＣＴＩＶＩＴＩＥＳ￥ＣｈａｎｇＪｕｎＳＵＮ；ＹｉＧｕｉＷＡＮＧ；ＺａｉＣｈｅｎｇＣＨＥＮ；ＰｅｎｇＸＵＥ；ＷｅｉＦｅｎｇＨＵ（Ｄｅｐ...     

SYNTHESIS AND PARTIAL CHARACTERIZATION OF WATER SOLUBLE RAPE POLLEN DODECAPEPTIDE

A dodecapeptide extracted from rape pollen was synthesized using solid—phase method,its conforma-tions in different states were studied by FT—IR,CD,and its physiological activities were determined.Sevensegments and five analogs were also synthesized to elucidate the structure—function relation.     

SYNTHESIS OF SOME NEUTRAL, WATERSOLUBLE CARBODIIMIDES AND THEIR USE IN THE FORMATION OF PEPTIDE BONDS

Two symmetrical (10a, 10b) and three unsymmetrical carbodiimides (10c, 10d, 10e) were synthesiz-ed from tris(β-methoxymethyl)ethylamine (1), bis(methoxymethyl)methylamine (2), and tris(methox-ymethyl)methylamine (3). They are neutral and readily soluble in many organic solvents including pe-troleum ether and possess sufficient solubilities in water except 10a which is less soluble. Their relativeabilities as condensing agent in the formation of peptide bonds were tested preliminarily by couplingphthalyl glycine and glycine ethyl ester in chloroform as well as in water and can be related to the stericeffects exerted by methoxymethyl-substituted alkyl residues with the order: tertiary>primary>secondary.1,3-Bis[bis(methoxymethyl)methyl] carbodiimide (10b) is promising as an all-soluble condensing agentfor the formation of peptide linkage and by its ready availability.     

THE SYNTHESIS AND 2D NMR STUDY OF DELTORPHINS

ＴＨＥＳＹＮＴＨＥＳＩＳＡＮＤ２ＤＮＭＲＳＴＵＤＹＯＦＤＥＬＴＯＲＰＨＩＮＳＴＨＥＳＹＮＴＨＥＳＩＳＡＮＤ２ＤＮＭＲＳＴＵＤＹＯＦＤＥＬＴＯＲＰＨＩＮＳ￥ＲｕｉＷＡＮＧ；ＸｉａｏＹｕＨＵ（ＮａｔｉｏｎａｌＬａｂｏｒａｔｏｒｙｏｆＡｐｐｌｉｅｄＯｒｇａ...     

SYNTHESIS AND SECRETION OF HUMAN ATRIAL NATRIURETIC PEPTIDE IN Saccharomyces cerevisiae

A chemically synthesized α-hANP gene was inserted into plasmid YFD18, which was an expression-secretion vector of yeast. The recombinant then transformed in the yeast Y33. The expression level of yeast transformants was about 700 μg ANP/L detected by RIA. More than 99% of expression products were secreted in the culture medium. N-terminal analysis of purified product showed that the first 4 amino acid residues of α-hANP were deleted.     

THE SYNTHESIS AND CHARACTERISTICS OF THE COORDINATION COMPOUNDS OF 2-MERCAPTOPYRIDINE-1-OXIDE WITH THE FIPST TRANSITION SERIES METALS

The stability of the coordination compounds of the first transitionseries metal ions(Mn(Ⅱ),Co(Ⅱ),Ni(Ⅱ),Cu(Ⅱ)and Zn(Ⅱ))with 2-mercaptopyridine-1-oxide is reported.A coordination compound CoL_2 is synthesized and characterizedfor the first time.     

THE ROLE OF RNA AND PROTEIN SYNTHESIZED BEFORE AND AFTER DEHISCENCE OF THE ANTHER IN THE DEVELOPMENT OF POLLEN GRAINS OF Clivia nobilis

Under the condition of completely inhibited RNA and protein synthesis, the rate of pollen grain germination of Clivia nobilis still reaches about 95% and the pollen tubes grow up to a certain length.RNase or pronase E added to the medium affected the germination and the elongation of the pollen tubes till they were completely blocked. Statistical results show that the original proteins may maintain the elongation of pollen tubes at about 70 μm, the proteins translated from pre-existing mRNA maintain them at about 530 μm.The RNA and protein synthesized after dehiscence of the anther are necessary for the normal rate of subsequent elongation and the growth of pollen tubes in the later stage (>2400 μm). Treatment of long duration such as CHM 3h and AmD 10h gives rise to aberration of pollen tubes, the rate of aberration being related to the concentration of inhibitors and the duration of treatment.In the first 5h of culture a suitable amount of inhibitors may block the progress of meta- phase of the gen     

THE SYNTHESIS OF TWO KINDS OF POLYMERSUPPORTED ASYMMETRIC PHASE TRANSFER CATALYSITS（PS—PTCS）AND THE COMPARISON OF THEIR CATALYTIC ACTIVITIES

In this paper,two kinds of PS-PTCs were synthesized using different methods by introducing cinchonine and quinine to the polymer support.Their catalytic properties for the alkylation of N-diphenylmethylene glycine t-butyl ester were also compared.     

SYNTHESIS OF DELTA SLEEP-INDUCING PEPTIDE(DSIP) AND ITS PHYSIOLOGICAL ACTIVITY

Protected nonapeptide—Delta Sleep-Induclng Peptide(DSIP)(Boc·Trp·Ala·Gly·Gly·Asp·Ala·Ser·Gly·Glu-OBu~t) has been synthesized by classical method. The product has been treated with TFA and purified on DEAE-Sephadex-A25 coltunn, pure free nonapeptide obtained and a to β transposition of Asp-residue found to be absent. It has been assayed by electrophoresis at pH 3.8, microcrystallinecellulose TLC and HPLC. The physiological activities of synthetic DSIP are performed on rabbits by using intravenous administration or mesodiencephalic ventricular infusion. Its function Of intensifying δ and σ waves on rabbit's electroencephalogram(EEG) is evident. There is no concomitant increase of δ- and σ-enhancing effect following mesodiencephalic ventricular infusion of 10 or 20 times higher than 5 μg/rabbit doses. Results of 6-day consecutive intravenous administration(50μg/kg) indicate that there is no obvious sign of adaptation to DSIP. Results suggest that the physiological function of endogenous sl     

STUDIES ON THE SYNTHESIS OF D-GLUCURONIC ACID DERIVATIVES OF 2-BUTOXY-5-FLUORO-3H-4-PYRIMIDONE AND THEIR ANTICANCER ACTIVITIES

2-Butoxy-5-fluoro-3H-4-pyrimidone derivatives of D-glucuronic acid having0-glycosidic linkage or N-glycosidic linkage were synthesized and their anticanceractivity tested.Their structures were confirmed by elementary analysis,IR spectraand ~1HNMR.     

THE SYNTHESIS AND STRUCTURE OF 4,5-DIOXO-2-THIO-1,3,2,4-DIAZADIPHOSPHOLANES

A new type of diphosphorus heterocycles, 4,5- dioxo- 2- thio- 1,3, 2,4- diazadiphospholanes,has been synthesized by the condensation of carbamyl phosphoryl amidate and 4- substitutedphenyl phosphine dichloride, and then sulfurization. The cis- and trans-isomers of the com-pounds were separated by HPLC, and their structures were studied with ~1H NMR and ~(31)PNMR. It has been found that the cis- and trans-isomers have different chemical shifts (δ) anddifferent coupling constants. The preliminary bioassay shows that some of them possessstrong herbicidal activity.     

THE SYNTHESIS AND CHARACTERIZATION OF A NOVELMIXED-VALENCE ISOPOLYVANADATE

ＴＨＥＳＹＮＴＨＥＳＩＳＡＮＤＣＨＡＲＡＣＴＥＲＩＺＡＴＩＯＮＯＦＡＮＯＶＥＬ　ＭＩＸＥＤ－ＶＡＬＥＮＣＥＩＳＯＰＯＬＹＶＡＮＡＤＡＴＥＪｉａＮｉｎｇＸＵ；ＧｕｏＹｕＹＡＮＧ；ＷｅｎＹｉｎｇＭＡ；ＺｈｉＺｈｏｎｇＳｕｎ；ＪｉＱｉｎｇＸＵ（Ｄｅｐａｒｔ...     

SYNTHESIS AND CHARACTERIZATION OF NOVEL FUNCTIONAL HOST COMPOUND 2~[1] MONO-

ＳＹＮＴＨＥＳＩＳＡＮＤＣＨＡＲＡＣＴＥＲＩＺＡＴＩＯＮＯＦＮＯＶＥＬＦＵＮＣＴＩＯＮＡＬＨＯＳＴＣＯＭＰＯＵＮＤ２~［１］ＭＯＮＯ－［６－Ｏ－（８－ＱＵＩＮＯＬＹＬ）］－β－ＣＹＣＬＯＤＥＸＴＲＩＮＹｕＬＩＵ；ＹｉＭｉｎＺＨＡＮＧ；ＹｕｎＴｉＣＨＥ?..