Fourier domain mode locking at 1050 nm for ultra-high-speed optical coherence tomography of the human retina at 236,000 axial scans per second

A Fourier domain mode-locked (FDML) laser at 1050 nm for ultra-high-speed optical coherence tomography (OCT) imaging of the human retina is demonstrated. Achievable performance, physical limitations, design rules, and scaling principles for FDML operation and component choice in this wavelength range are discussed. The fiber-based FDML laser operates at a sweep rate of 236 kHz over a 63 nm tuning range, with 7 mW average output power. Ultra-high-speed retinal imaging is demonstrated at 236,000 axial scans per second. This represents a speed improvement of approximately10x over typical high-speed OCT systems, paving the way for densely sampled volumetric data sets and new imaging protocols.     

基于法布里-珀罗调谐滤波器的傅里叶域锁模扫频激光光源

报道了一个光纤型1300 nm波段的傅里叶域锁模(Fourier domain mode locking, FDML)扫频激光光源, 用于扫频光学相干层析成像技术 (optical coherence tomography, OCT) 成像. 本实验扫频激光光源采用包含增益介质、调谐滤波器和延迟线组成的长腔激光谐振腔以及光功率增强单元. FDML扫频激光光源具有快速和高度稳定运转模式, 相位稳定性好. 基于法布里-珀罗调谐滤波器(fiber Fabry-Perot tunable filter, FFP-TF)的FDML扫频激光光源扫频范围为130 nm, 半高全宽为70 nm, 输出平均功率是11 mW. 与基于FFP-TF的短腔的扫频光源做了对比研究, FDML扫频光源速度从短腔的8 kHz提高到了48.12 kHz, 对应生物组织OCT成像轴向分辨率为7.8 μm, 比短腔的减小了1.9 μm. 关键词： 光学相干层析技术 扫频激光光源 傅里叶域锁模 法布里-珀罗调谐滤波器     

Phase-sensitive optical coherence tomography at up to 370,000 lines per second using buffered Fourier domain mode-locked lasers

Buffered Fourier domain mode-locked (FDML) lasers are demonstrated for dynamic phase-sensitive optical coherence tomography (OCT) and 3D OCT phase microscopy. Systems are operated at sweep speeds of 42, 117, and 370 kHz, and displacement sensitivities of 39, 52, and 102 pm are achieved, respectively. Sensitivities are comparable to spectrometer-based OCT phase microscopy systems, but much faster acquisition speeds are possible. An additional factor of sqrt 2 improvement in noise performance is observed for differential phase measurements, which is important for Doppler OCT. Dynamic measurements of piezoelectric transducer motion and static 3D OCT phase microscopy are demonstrated. Buffered FDML lasers provide excellent displacement sensitivities at extremely high sweep speeds.     

High-speed, high-resolution optical coherence tomography retinal imaging with a frequency-swept laser at 850 nm

High-speed, high-resolution optical coherence tomography (OCT) imaging of the human retina is demonstrated using a frequency-swept laser at 850 nm. A compact external cavity semiconductor laser design, optimized for swept-source ophthalmic OCT, is described. The laser enables an effective 16 kHz sweep rate with >10 mm coherence length and a tuning range of approximately 35 nm full width at half-maximum, yielding an axial resolution of <7 micro m in tissue.     

Visualization of hair follicles using high-speed optical coherence tomography based on a Fourier domain mode locking laser

In this study, a swept-source optical coherence tomography (SS-OCT) system with a Fourier domain mode locking (FDML) laser is proposed for a dermatology study. The homemade FDML laser is one kind of frequency-sweeping light source, which can provide output power of >20 mW and an output spectrum of 65 nm in bandwidth centered at 1300 nm, enabling imaging with an axial resolution of 12 μm in the OCT system. To eliminate the forward scans from the laser output and insert the delayed backward scans, a Mach-Zehnder configuration is implemented. Compared with conventional frequency-sweeping light sources, the FDML laser can achieve much higher scan rates, as high as ∼240 kHz, which can provide a three-dimensional imaging rate of 4 volumes/s. Furthermore, the proposed high-speed SS-OCT system can provide three-dimensional (3D) images with reduced motion artifacts. Finally, a high-speed SS-OCT system is used to visualize hair follicles, demonstrating the potential of this technology as a tool for noninvasive diagnosis of alopecia.     

Polarization-maintaining buffered Fourier domain mode-locked swept source for optical coherence tomography

A polarization-maintaining buffered Fourier domain mode-locked (FDML) swept source with a center wavelength of 1300 nm is demonstrated. The scanning rate of the buffered FDML swept source is doubled without sacrificing the output power of the swept source by combining two orthogonally polarized outputs with a polarization beam combiner. The stability of the swept source is improved because the polarization state of the laser beam inside the laser cavity is maintained without the use of any polarization controllers. The swept source is capable of an edge-to-edge tuning range of more than 150 nm and a FWHM range of 95 nm at a 102 kHz sweeping rate and with an average power of 12 mW. A swept source optical coherence tomography (SSOCT) system is developed utilizing this buffered FDML swept source. The axial resolution of the SSOCT system is measured to be 9.4 μm in air. The sensitivity of the SSOCT system is 107.5 dB at a depth of 0.25 mm with a 6 dB roll-off at a depth of 2.25 mm.     

Sound localization of frequency-modulated sinusoids by Old World monkeys

Directional hearing acuity, as measured by the minimum audible angle (MAA), was determined in four Old World monkeys, Macaca radiata. The acoustic stimuli were linear changes in frequency (sweeps) for different frequency ranges and sweep rates. The sweeps ranged between 0.5 and 1.3 kHz, at two durations, 100 and 200 ms. In upsweeps which began at 0.5 kHz and were 200 ms in duration, MAA decreased as sweep rate and frequency range increased. These thresholds were compared to MAAs of sweeps which traversed the same range of frequencies but at a different rate, to MAAs of sweeps with identical rates but over different frequency ranges, and to the MAAs of downsweeps. These comparisons indicated that range, and not sweep rate, exerts the greatest effect on the MAA. Interaural phase differences derived from the upper limits of the frequency range are discussed as potential FM localization cues.     

Broadband and high-speed swept external-cavity laser using a quantum-dot superluminescent diode as gain device

A wide wavelength tuning range swept external-cavity laser using an In As/Ga As quantum-dot superluminescent diode as a gain device is demonstrated. The tunable filter consists of a polygon scanner and a grating in Littrow telescope-less configuration. The swept laser generates greater than 54-m W peak output power and up to 33-k Hz sweep rate with a sweep range of 150 nm centered at 1155 nm. The effects of injection current and sweep rate on the sweep performance of the swept laser are studied.     

Theoretical study on instantaneous linewidth of Fourier-domain mode-locked fiber lasers

We have numerically simulated the operation of the Fourier-domain mode-locked (FDML) fiber laser based on the wavelength reconstruction method instead of numerical solving the nonlinear Schrödinger equation. We studied the influences of the filter bandwidth and the relative time delay caused by the fiber chromatic dispersion on the instantaneous linewidth of the FDML fiber laser. The results show that the instantaneous linewidth broadens as the filter bandwidth and the relative time delay increase. When the filter has the bandwidth of 0.02 nm, the narrowest and broadest instantaneous linewidths are 0.024 and 0.042 nm, respectively. We give an understanding for the oscillation of the instantaneous linewidth of FDML. The presented result can be used to evaluate the performance achievable in the FDML fiber lasers.     

Characterization of an actively linearized ultrabroadband chirped laser with a fiber-laser optical frequency comb

The optical frequency sweep of an actively linearized, ultrabroadband, chirped laser source is characterized through optical heterodyne detection against a fiber-laser frequency comb. Frequency sweeps were measured over approximately 5 THz bandwidths from 1530 nm to 1570 nm. The dominant deviation from linearity resulted from the nonzero dispersion of the fiber delay used as a reference for the sweep linearization. Removing the low-order dispersion effects, the residual sweep nonlinearity was less than 60 kHz rms, corresponding to a constant chirp with less than 15 ppb deviation across the 5 THz sweep.     

Remote fiber sensor based on cascaded Fourier domain mode-locked laser

We propose a high-speed remote fiber Bragg grating (FBG) sensor interrogation system based on a 1.3-μm cascaded Fourier-domain mode-locked (FDML) laser. It consists of multiple FBGs connected to an optical circulator in the laser cavity. The cascaded FDML laser with these multiple FBGs is operational when the scanning frequency of the fiber Fabry-Perot tunable filter matches the fundamental frequency of the laser cavity. Each FBG provides a separate laser cavity for the FDML laser. The scanning frequencies of each laser cavity are 30.5314, 31.5393, 32.7108, and 33.8023 kHz. Using the cascaded FDML laser, we measure the performance of the long-distance static strain FBG sensor interrogation system in both the time and spectral domains. The slope coefficients of the measured relative wavelength difference and the relative time delay from the static strain are found to be 0.95 pm/μstrain and 0.15 ns/μstrain, respectively. We also demonstrate the dynamic response of the interrogation system with 80-Hz modulation strain using the cascaded FDML laser. Thus, an FBG sensor interrogation system for high-speed and high-sensitivity long-distance monitoring systems can be realized using a cascaded FDML laser.     

Simultaneous dual-wavelength-band common-path swept-source optical coherence tomography with single polygon mirror scanner

We report a novel (to the best of our knowledge) simultaneous 1310/1550 two-wavelength band swept laser source and dual-band common-path swept-source optical coherence tomography (SS-OCT). Synchronized dual-wavelength tuning is performed by using two laser cavities and narrowband wavelength filters with a single dual-window polygonal scanner. Measured average output powers of 60 and 27 mW have been achieved for the 1310 and 1550 nm bands, respectively, while the two wavelengths were swept simultaneously from 1227 to 1387 nm for the 1310 nm band and from 1519 to 1581 nm for the 1550 nm band at an A-scan rate of 65 kHz. Broadband wavelength-division multiplexing is used for coupling two wavelengths into a common-path single-mode GRIN-lensed fiber probe to form dual-band common-path SS-OCT. Simultaneous OCT imaging at 1310 and 1550 nm is achieved. This technique allows for in vivo high-speed OCT imaging with potential application in functional (spectroscopic) investigations.     

Fourier domain mode locking pulse fiber laser

A novel pulse fiber laser is proposed based on the modulation of the lasing wavelength. Different from conventional amplitude or phase modulated mode-locking fiber lasers, the proposed fiber laser is with a so-called Fourier domain mode locking (FDML) operation (i.e., the cavity round-trip time of the optical pulse should match the period that the open time window of the tunable filter appears for the lasing wavelength or its harmonics). An FDML pulse fiber laser with a cavity round-trip frequency of 19.46 kHz is demonstrated and optical pulses with repetition rate of 19.46 kHz are achieved when the wavelength modulation frequency is 9.73 kHz.     

Dual-core ytterbium fiber amplifier for high-power 1060 nm swept source multichannel optical coherence tomography imaging

A novel (to our knowledge) dual-core ytterbium (Yb(3+)) doped fiber, as an optically pumped amplifier, boosts the output power from a 1060 nm swept source laser beyond 250 mW, while providing a wavelength tuning range of 93 nm, for optical coherence tomography (OCT) imaging. The design of the dual-core Yb-doped fiber amplifier and its multiple wavelength optical pumping scheme to optimize output bandwidth are discussed. Use of the dual-core fiber amplifier showed no appreciable degradation to the coherence length of the seed laser. The signal intensity improvement of this amplifier is demonstrated on a multichannel in vivo OCT imaging system at 1060 nm.     

Fourier domain optical coherence tomography using optical demultiplexers imaging at 60,000,000 lines/s

We describe high-speed Fourier domain optical coherence tomography (OCT) using optical demultiplexers (ODs) for spectral dispersion. The OD enables separation of a narrow spectral band of 14 GHz (0.11 nm) from a broadband incident light at 256 different frequencies in 25.0 GHz intervals centered at 192.2 THz (1559.8 nm). OCT imaging of 60,000,000 axial scans per second was achieved through parallel signal acquisition using 256 balanced photoreceivers to simultaneously detect all the output signals from the ODs in a Fourier domain OCT system. OCT imaging at a 16 kHz frame rate, 1100 A-lines per frame, 3 mm depth range, and 23 microm resolution was demonstrated using a resonant scanner for lateral scanning.     

Quasi-distributed fiber Bragg grating sensor system based on a Fourier domain mode locking fiber laser

A novel quasi-distributed fiber Bragg grating (FBG) sensor system based on Fourier domain mode locking (FDML) fiber laser is proposed and demonstrated. The low reflectivity FBGs with the same Bragg wavelength are connected cascaded in a long fiber working as the sensing elements of the sensor system as well as the wavelength and cavity length selecting elements of the FDML laser. By adjusting the driving frequency of the FDML fiber laser, lasing with different selected cavity lengths will be achieved correspondingly. When the wavelength of the working FBG shifts which includes the sensing information, FBG interrogation can be realized both in wavelength and time domain.     

In vivo Fourier-domain full-field OCT of the human retina with 1.5 million A-lines/s

In vivo full-field (FF) optical coherence tomography (OCT) images of human retina are presented by using a rapidly tunable laser source in combination with an ultra-high-speed camera. Fourier-domain FF-OCT provided a way to increase the speed of retinal imaging by parallel acquisition of A-scans. Reduced contrast caused by cross talk was observed only below the retinal pigment epithelium. With a 100Hz sweep rate, FF-OCT was fast enough to acquire OCT images with acceptable motion artifacts. FF-OCT allows ultrafast retinal imaging, boosting image speed by a lack of moving parts and a considerably higher irradiation power.     

Full range complex ultrahigh sensitive optical microangiography

This Letter presents a useful method that combines the full range complex Fourier domain optical coherence tomography (OCT) with the ultrahigh sensitive optical microangiography (OMAG) to achieve full range complex imaging of blood flow within microcirculatory tissue beds in vivo. We propose to use the fast scanning axis to realize the full range complex imaging, while using the slow axis to achieve OMAG imaging of blood flow. We demonstrate the proposed method by using a high speed 1310?nm OCT/OMAG system running at 92?kHz line scan rate to image the flow phantoms in vitro, and the blood flows in tissue beds in vivo.     

In vivo ultrahigh-resolution optical coherence tomography

Ultrahigh-resolution optical coherence tomography (OCT) by use of state of the art broad-bandwidth femtosecond laser technology is demonstrated and applied to in vivo subcellular imaging. Imaging is performed with a Kerr-lens mode-locked Ti:sapphire laser with double-chirped mirrors that emits sub-two-cycle pulses with bandwidths of up to 350 nm, centered at 800 nm. Longitudinal resolutions of ~1mum and transverse resolution of 3mum, with a 110-dB dynamic range, are achieved in biological tissue. To overcome depth-of-field limitations we perform zone focusing and image fusion to construct a tomogram with high transverse resolution throughout the image depth. To our knowledge this is the highest longitudinal resolution demonstrated to date for in vivo OCT imaging.     

Probing fermionic condensates by fast-sweep projection onto Feshbach molecules

Fast-sweep projection onto Feshbach molecules has been widely used as a probe of fermionic condensates. By determining the exact dynamics of a pair of atoms in time-varying magnetic fields, we calculate the number of condensed and noncondensed molecules created after fast magnetic field sweeps from the BCS to the Bose-Einstein condensate side of the resonances in 40K and 6Li, for different sweep rates and a range of initial and final fields. We discuss the relation between the initial fermionic condensate fraction and the molecular condensate fraction measured after the sweep.