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铁-邻二氮杂菲间接分光光度法测定织物中游离甲醛 总被引:16,自引:0,他引:16
1引言微量甲醛的测定方法很多,其中较为简便的是分光光度法,常用的有乙酰丙酮法、变色酸法等,但这些方法灵敏度不高,其最大吸收波长处。分别为7.2×103和2.1×103L·mol-1·cm-1,显色条件苛刻。本文研究了题示的测定方法,基本原理是:碱性条件下,甲醛与水合氧化银发生氧化还原反应,生成的银定量还原铁(Ⅲ)为铁(ⅱ),铁与邻二氮来菲形成稳定的桔红色给合物。此络合物的本法是现有甲醛光度测定法中较灵敏的。方法用于织物样品中游离甲醇的测定,取得了满意结果。2实验部分2.1仪器与试剂721型分光光度计(上海第三分析仪器厂)… 相似文献
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甲醛是一种危害人们身体健康的化学物质.生活中甲醛污染无处不在,室内装饰的各种人造板材、贴墙布、贴墙纸、化纤地毯、泡沫塑料、油漆和涂料等均含有甲醛,并会逐渐向周围环境释放;使用了树脂整理剂或涂层的织物面料、服装也含有甲醛成分,直接接触人体损害人们的健康. 相似文献
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在HAc-NaAc缓冲介质中,盐酸氯丙嗪(CPZ)能定量使Fe(Ⅲ)还原为Fe(Ⅱ),还原生成的Fe(Ⅱ)与邻二氮菲反应生成稳定的红色络合物,并且在一定范围内,CPZ的浓度和生成的红色络合物的吸光度呈良好的线性关系。据此,提出邻二氮菲-Fe(Ⅲ)体系测定盐酸氯丙嗪的新方法。在优化的实验条件下,盐酸氯丙嗪的质量浓度在0.040~15.00 mg/L范围内与吸光度呈现良好的线性关系,线性相关系数R=0.9995,摩尔吸光系数ε=2.8×104 L.mol-1.cm-1,检出限为0.020 mg/L。11次重复测定的相对标准偏差小于2.9%。该方法用于药物中盐酸氯丙嗪的测定,结果满意。 相似文献
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邻羟基苯基重氮氨基偶氮苯分光光度法测定地质样品中的痕量铊 总被引:3,自引:0,他引:3
1引言 铊是稀散元素,在制造电子元器件、化学、玻璃及半导体工业等领域有重要用途。铊也是剧毒元素,其毒性为氧化砷的3倍多。随着铊的使用日益增多,它的环境效应引起了人们的关注,因此铊的分析工作越来越重要。近年来,水相胶束增溶分光光度法测定铊的研究日趋增加。邻羟基苯基重氮氨基偶氯苯(O-HDAA)曾用于锌、银、镉、镍、钴等的光度分析。本文详细研究了在 Triton X-100存在下,O-HDAA与铊的高灵敏显色反应。聚胺酯泡沫塑料对铊有很好的吸附性能,本文用于分离富集铊。建立了一个在水相体系中快速、准确… 相似文献
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建立乙酰丙酮分光光度法测定土壤中甲醛含量的分析方法。在磷酸介质中,土壤中的甲醛经过加热蒸馏提取,与乙酰丙酮反应生成黄色的二乙酰基二氢二甲基吡啶,然后于412 nm波长处测定其吸光度。甲醛含量在0.0~25μg范围内与吸光度呈良好的线性关系,线性相关系数r=0.999 6,加标回收率为76.2%~87.5%,方法检出限为0.03 mg/kg,定量限为0.12 mg/kg,测定结果的相对标准偏差为2.73%~3.68%(n=6)。该方法灵敏度高,分析速度快,适用于土壤中甲醛含量的测定。 相似文献
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在中性或弱酸性中,氨存在条件下亚硫酸盐与邻苯二甲醛生成强荧光物质,而过氧化苯甲酰对该荧光物质具有抑制作用。基于该原理建立了一种简单、灵敏测定面粉中微量过氧化苯甲酰的荧光光度法。荧光物质的最大激发波长和发射波长分别为326、389 nm。方法检出限为0.23μg/mL,线性范围为0.8~8.0μg/mL,线性回归方程为ΔF=20.589c 2.8805,相关系数r=0.999 0。该法用于测定面粉中微量过氧化苯甲酰含量的相对标准偏差为1.34%~2.32%,回收率为95.4%~101.1%。 相似文献
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硝基磺酚C光度法测定蛋白质的研究 总被引:19,自引:0,他引:19
蛋白质的定量分析是生化研究、临床化验和食品检验等领域经常涉及的内容.以有机小分子作光谱探针测定蛋白质,如甲基橙[1,2]、考马斯亮蓝G-250[3]、溴酚蓝[4]、溴甲酚绿[5]和偶氮胂Ⅲ[6]等已得到研究. 相似文献
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以锌试剂显色法测定蛋白质的研究 总被引:19,自引:1,他引:19
有关蛋白质染色测定的分光光度研究已有不少报道,其中有些已用于蛋白质的分析测定[1~4].锌试剂常用于金属离子的分光光度法测定,但作为生物大分子分析试剂的研究,目前还未见报道.实验表明,在pH4左右的缓冲溶液中,锌试剂与BSA反应生成有色复合物,吸收光... 相似文献
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伊红Y分光光度法测定血清白蛋白 总被引:8,自引:0,他引:8
在 p H 3.2 9缓冲介质中 ,牛血清白蛋白与伊红 Y结合 ,形成 EY- BSA复合物 ,以试剂空白参比 ,在 5 4 5 nm处产生一灵敏的吸收峰 ,BSA的浓度在 0~ 1 .6×1 0 - 7mol/L范围内与 5 4 5 nm处的吸光度呈良好的线性关系 ,摩尔吸光系数 ε=2 .1 2× 1 0 6 L· mol- 1· cm- 1,Sandell灵敏度为 0 .0 32 μg/cm2 ,方法对 BSA的检出限为9.2× 1 0 - 9mol/L。本法具有灵敏度高、选择性好等特点 ,用于人血清样品中蛋白质的测定 ,与经典的考马斯亮蓝 G- 2 5 0方法结果一致 相似文献
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《Analytical letters》2012,45(10):1799-1809
Abstract Two acid dye reagents have been utilized for spectrophotometric determination of triamterene in pure form and in pharmaceutical preparations. The dyes used are bromophenol blue (B. P. B), and bromothymol blue (B. T. S. They form a chloroform-soluble, coloured ion association complex with triamterene, at pH 3.4 and 3.2 using B. P. B and B. T. B, respectively. The formed complex could be extracted and measured spectrophotometrically at 417 nm for both dyes. The molar ratio of the formed drug-dye ion association complex is found to be 1:1 for both dyes used, as deduced by applying Job's method. Linearity was obtained using concentration ranges of 2.5–10 μg, and 2.5–15 μg. ml?1 of triamterene with B. P. B. and B. T. B. respectively. The validity of the procedure was assessed by applying the standard addition technique. Results obtained by using the proposed procedure were statistically analysed and compared with those obtained by adopting the pharmacopoeial method. 相似文献
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《Analytical letters》2012,45(11-12):1289-1296
Abstract A new sensitive and seleactive method for rapid and accurated determination of nitrofarazone is described. The nitrofurazone is reacted with hydroxyl-amine hydrochloride in alkaline medium to give hydroxamic acid which forms the purple violet coloured complex with iron (III), FeCl3, in acidic medium. The nitrofurazone was determined in presence of commonly used drugs and additives. The results obtained by the proposed and B.P. methods were compared. 相似文献
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A simple, sensitive and accurate spectrophotometric method has been described for the determination of ampicillin(I), amoxicillin trihydrate(II) and cefazolin sodium(III). The procedure is based on the formation of Prussian Blue (PB) complex. The reaction between the acidic hydrolysis products of antibiotics (T = 60 °C) with mixture of Fe3+ and hexacyanoferrate(III) ions was evaluated for the spectrophotometric determination of the mentioned drugs. The maximum absorbance of the colored complex occurs at λ = 700 nm and the molar absorptivity is 3.0 × 104 1 mol?1cm?1. The effect of various parameters such as concentration of K3Fe(CN)6 and Fe3+, nature and amount of acids used, temperature and time of heating were investigated. Under optimum conditions the linear range of calibration graph was 2.0–12.0, 5.0–13.5 and 3.0–12.0 μg mL?1 for ampicillin, amoxicillin and cefazolin, respectively. The relative standard deviation for the determination of 10 μg mL?1 of antibiotics was about 0.5–1.5%. The proposed method was successfully applied to the determination of selected antibiotics from pharmaceutical preparations. The validity of the method was tested by the official methods and by the recovery studies of standard addition to pharmaceuticals. 相似文献
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《Analytical letters》2012,45(14):3037-3050
ABSTRACT A simple and very sensitive method for the determination of formaldehyde is described. Formaldehyde reacts with phloroglucinol in acid medium producing a yellow dye with λmax at 435nm. The method is useful for the determination of formaldehyde in the range 0-2.5μg in an overall volume of 10mL with molar absorptivity of 8.3 × 1041 mol?1 cm?1 and a relative standard deviation of 2% at 1.5μg level (n=10). 相似文献