Lipids of Hibiscus cannabinus seeds

The neutral and polar lipids of kenaf seeds of the variety Uzbetskii-1574 from the 1983 crop have been studied. The fatty acid compositions of six acyl-containing classes of neutral lipids have been established for the first time. The acids in the sn-2 positions of the triacylglycerols and of the main classes of phospholipids have been determined. The main triacylglycerol species are those in which the sn-2 position is esterified by linoleic acid.     

Neutral lipids ofCitrullus vulgaris seeds

The qualitative and quantitative compositions of the neutral lipids of watermelon seeds have been studied. The fatty acid compositions of the acyl-containing lipids have been determined. In the triacylglycerols the main forms are those in which the sn-2 position is substituted by linoleic acid.Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 494–495, May–June, 1995. Original article submitted May 30, 1994.     

Lipid complex of Peganum harmala

The lipid complex of the seeds ofPeganum harmala (fam. Zygophyllaceae) has been investigated. The qualitative and quantitative compositions of the neutral lipids and the phospholipids have been studied. The fatty acid compositions of the acyl-containing lipids have been determined and it has been shown that in the triacylglycerols and phosphatidylethanolamines the sn-2 position is esterified mainly by linoleic acid.Institute of Chemistry of Plant Substances, Uzbekistan Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 625–627, November–December, 1992.     

Lipids ofZizyphus jujuba

The acyl-containing classes of neutral lipids have been studied and the amounts of phospholipids and their fatty acid composition have been determined in the pericarps and seeds ofZizyphus jujuba. The fatty oil of the seeds contains oleic acid while in the neutral lipids of the pericarps the main acid is the biologically active palmitoleic. In the qualitative respect, the set of phospholipids in the seeds is more diverse, while the main classes quantitatively in both parts of the fruit are phosphatidylcholines and phosphatidylglycerols. The phospholipids of the pericarps are more saturated than those of the seeds.Institute of Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 21–24, January–February, 1990.     

The lipids ofHibiscus seeds

The composition and amounts of various classes of lipids of the seeds of Hibiscus sp. have been investigated. Seven classes of neutral lipids, five of phospholipids, and five of glycolipids have been identified and their fatty-acid and triglyceride compositions have been established. The presence has been shown of 16 main types of triacylglycerols and 25 types of epoxyacyldiacylglycerols. An epoxy-18.0 and an epoxy-18:1 acid in a combined amount of 1.4% have been detected among the acids of the free lipids, together with 8.1% of cyclopropenoid acids.Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 40–44, January–February, 1995. Original article submitted October 24, 1994.     

Lipids ofHeracleum lehmannianum seeds

The lipid complex of the seeds ofHeracleum lehmannianum B. has been studied. The qualitative and quantitative compositions of the neutral lipids and the phospho- and glycolipids, and the fatty acid compositions of the acyl-containing classes of lipids have been determined. It has been shown that the neutral and the polar lipids differ considerably from one another with respect to the level of certain fatty acids. The position-species compositions of the TAGS and of the main components of the phospholipids have been calculated from the results of lipolytic hydrolysis.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 89 14 75. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 10–14, January–February, 1996. Original article submitted June 12, 1995.     

Lipids ofGossypium mexicanum

The surface lipids of the coat and the lipids of the kernel of the seeds of a wild form of the medium-fibered cotton plantGossypium mexicanum var.nervosum have been studied in comparison with cultured species. Using the methods of CC, TLC, and GLC, and UV and mass spectrometry, in the cuticular lipids ofG. mexicanum three classes of neutral lipids have been detected, and in the kernel lipids 10 classes of neutral lipids, 2 classes of glycolipids, and 7 classes of phospholipids. The structures of the epoxyacyl-, hydroxyacyl-, and diacylglycerols of the kernel have been established. The greatest differences between the seeds of the wild form of the cotton plant and the cultured species are the greater weight of the seed coat, the presence in it of a considerable amount of the 18:1 and of medium-molecular-weight fatty acids, and a higher level in the kernel of gossypol pigments, polar lipids, and acylglycerols containing the 18:2 acid.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 57–62, January–February, 1987.     

Composition of the neutral lipids of Chlorella vulgaris

Summary The qualitative and quantitative compositions of the neutral lipids and the fatty-acid composition of the individual classes of neutral lipids isolated from fresh, freeze-dried, and air-dry cells of the algaChlorella vulgaris have been investigated.The presence of natural fatty acid methyl esters in the lipid extract of the fresh and air-dry chlorella cells has been established.It has been shown that the fatty acid methyl ester fraction is enriched with palmitic acid as compared with the triglyceride and free fatty acid fractions.The main unsaturated acids of the chlorella lipids contain the first double bond in the ⁹ position.Division of Microbiology of the Academy of Sciences of the Uzbek SSR, Tashkent. Institute of the Chemistry of Plant Substances, Academy of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 49–53, January–February, 1978.     

Lipids of the fruit of Citrus unshiu

The compositions and the amounts of various groups of liposoluble compounds in the component elements of the fruits ofCitrus unshiu Marc. [satsuna variety of mandarin orange] — the flavedo, the albedo, the membrane, and the flesh — have been determined by chromatographic and chemical methods. About 30 groups of lipid compounds belonging to the classes of neutral lipids and glyco- and phospholipids have been identified and characterized. The fatty-acid compositions of the main classes of lipids have been studied. About 30 different carotenoids have been determined in the various elements of the satsuma orange.M. V. Lomonosov Odessa Technological Institute of the Food Industry. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 342–348, May–June, 1989.     

Lipids from extracts ofChlorella vulgaris

The composition of ethanolic (I) and gasoline (II) extracts of the cultivated microalgaChlorella vulgaris has been studied. With the aid of CC, TLC, qualitative reactions, GLC, and UVS the following classes of compounds have been detected in them. From the neutral lipids: hydrocarbons, carotenoids, traces of sterols and their esters, fatty acid esters, tri- and diacylglycerols, free fatty acids, and chlorophylls; and from the polar lipids: di- and monogalactosylglycerols, phosphatidylethanolamine, lecithin, phosphatidylinositol, phosphatidylserine, and three sphingosine bases. The polar lipids I and II made up 52.4 and 50.2% of the total, respectively. As compared with extract I, extract II was somewhat enriched with neutral lipids, including provitamins of the A group and vitamins of the F group. In the fatty acids of chlorella, 19 components were detected, the main ones being the 16:0 acid and 18:2 and 18:3 acids.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 284–288, May–June, 1986.     

Polar lipids of cotton seeds and the products of their processing

The compositions and amounts of polar lipids in cotton seeds and the products of their processing have been investigated. Six glycolipids have been identified, the main ones being monogalactosyldiglyceride (MGDGs), digalactosyldiglycerides (DGDGs), and sterol glycosides. The fatty acid compositions of the neutral lipids and of the phospho- and glycolipids have been determined. In the glycolipids, saturated fatty acids — palmitic and stearic — predominated.Institute of Chemistry of Plant Substances, Uzbekistan Republic Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 194–198, March–April, 1993.     

Metabolites of the pathogenic fungusVerticillium dahliae III. The lipid composition ofVerticillium dahliae

Summary The compositions of the neutral lipids of the mycelium and of the culture liquid from the pathogenic fungusVerticillium dahliae Kleb. have been investigated for the first time. The fatty-acid compositions of the main lipid fractions and the glyceride composition of the triglyceride fraction have been determined. The main acids of all the fractions are the 16:0 and 18:1 acids and in the triglyceride fraction, additionally, the 18:2 acid. Diisobutyl phthalate has been found in the combined neutral lipids of the fungal mycelium.V. I. Lenin Tashkent State University, Institute of the Chemistry of Plant Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 431–435, July–August, 1976.     

Regiospecific analysis of neutral ether lipids by liquid chromatography/electrospray ionization/single quadrupole mass spectrometry: validation with synthetic compounds.

A reversed-phase high-performance liquid chromatography (HPLC) method with on-line electrospray ionization/collision-induced dissociation/mass spectrometry (ESI/CID/MS) is presented for the regiospecific analysis of synthetic reference compounds of neutral ether lipids. The reference compounds were characterized by chromatographic retention times, full mass spectra, and fragmentation patterns as an aid to clarify the regiospecificity of ether lipids from natural sources. The results clearly show that single quadrupole mass spectroscopic analysis may elucidate the regiospecific structure of neutral ether lipids. Ether lipid reference compounds were characterized by five to six major ions in the positive ion mode. The 1-O-alkyl-sn-glycerols were analyzed as the diacetoyl derivative, and showed the [M - acetoyl](+) ion as an important diagnostic ion. The diagnostic ions of directly analyzed 1-O-alkyl-2-acyl-sn-glycerols and 1-O-alkyl-3-acyl-sn-glycerols were the [M - alkyl](+), [M + H - H(2)O](+) and [M + H](+) ions. Regiospecific characterization of the fatty acid position was evident from the relative ion intensities, as the sn-2 species had relatively high [M + H](+) ion intensities compared with [M + H - H(2)O](+), whereas the reverse situation characterized the sn-3 species. Furthermore, corresponding sn-2 and sn-3 species were separated by the chromatographic system. However, loss of water was promoted as fatty acid unsaturation was raised, which may complicate interpretation of the mass spectra. The diagnostic ions of directly analyzed 1-O-alkyl-2,3-diacyl-sn-glycerols were the [M - alkyl](+), [M - sn-2-acyl](+) and [M - sn-3-acyl](+) ions. Regiospecific characterization of the fatty acid identity and position was evident from the relative ion intensities, as fragmentation of the sn-2 fatty acids was preferred to the sn-3 fatty acids; however, loss of fatty acids was also promoted by higher degrees of unsaturation. Therefore, both structural and positional effects of the fatty acids affect the spectra of the neutral ether lipids. Fragmentation patterns and optimal capillary exit voltages are suggested for each neutral ether lipid class. The present study demonstrates that reversed-phase HPLC and positive ion ESI/CID/MS provide direct and unambiguous information about the configuration and identity of molecular species in neutral 1-O-alkyl-sn-glycerol classes.     

Effect of a wilt infection on the neutral lipids of cotton leaves

The composition of the neutral lipids of leaves of a cotton plant of the wilt-resistant variety 175-F, the set of components in the fatty acid composition of the lipid classes, and the partial structures of the triacylglycerides have been determined. The changes taking place in the composition and structure of these lipids on artificial infection of the plant by the fungal pathogenVerticillium dahliae have been elucidated.Institute of the Chemistry of Plant Substances, Uzbekistan Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 477–483, September–October, 1992.     

Lipids of the bark ofElaeagnus angustifolia branches

The compositions of the neutral lipids and the glyco- and phospholipids of the bark of branches ofElaeagnus angustifolia (fam. Elaeagnaceae) have been investigated. The fatty acid compositions of the acyl-containing classes have been established.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 40 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 41–44, January–February, 1998.     

Lipids ofPhellodendron lavalei seeds

The chemical composition of neutral lipids and phospholipids from seeds ofPhellodendron lavalei cork is studied. Characteristic features of the fine structure of the separate classes are found. Significant quantities of eicosatrienoic acid and neutral lipids are found. The polar lipids are biologically active.Deceased.I. G. Kutateladze Institute of Pharmacochemistry, Academy of Sciences of Georgia, Tbilisi, fax (99532) 25 00 26. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 220–222, May–June, 2000.     

Lipids of some aquatic plants of the Central Volga region

The lipid characteristics of five species of aquatic plants of the Central Volga region have been investigated. The total amount of lipids and the ratio of neutral lipids and glyco- and phospholipids have been found and the amounts of individual phospholipids and also the fatty-acid composition and the distribution of the fatty acids over the lipid classes have been determined.Institute of the Ecology of the Volga Basin, Russian Academy of Sciences, Tol'yatti. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 206–209, March–April, 1995. Original article submitted August 15, 1994.     

Lipids of the fruit of Ficus carica

By chromatographic methods, about 30 groups of various lipid compounds belonging to the classes of neutral lipids, glycolipids, and phospholipids have been identified from the fruit of the fig tree. The main groups are triacylglycerols, free and esterified sterols, mono- and digalactosyldiglycerides, ceramide oligosides, cerebrosides, esterified sterol glycosides, and phosphatidylglycerols. In the fatty acid composition, linoleic, linolenic, oleic, and palmitic acids predominated (>90%).A. V. Bogatskii Physicochemical Institute, Academy of Sciences of the Ukrainian SSR, Odessa. Lomonosov Technological Institute of the Food Industry, Odessa. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 423–427, July–August, 1986.     

Lipids of two species of brown algae of the genusLaminaria

The compositions of the neutral lipids, glycolipids, phospholipids and fatty acids of the brown algaeLaminaria japonica andLaminaria cichoriodes have been studied. It has been found that the glycolipids are the main lipid classes in these algae. Palmitic, oleic, and polyunsaturated acids with 18 and 20 carbon atoms form the group of main acids. The lipids of both species of theLaminaria genus have a complex composition but they differ by the ratios of their fatty acids.Institute of Marine Biology, Far-eastern Scientific Center, Russian Academy of Sciences, Vladivostok, fax (4232) 310900, e-mail: lipid@biom.marine.su. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 22–26, January–February, 1999.     

Lipids of elaeagnus fruit

The composition of the classes of lipids of the fruit of three morphological forms of the elaeagnusElaeagnus angustifolia L. have been studied. Their compositions were identical. The amounts of the main lipid classes of extracts of the seeds and pericarps, the fatty acid compositions of the acyl-containing classes of lipids, and the compositions of the carbohydrates and sterols have been determined. The fatty oil of the seeds contained linoleic acid, while the main fatty acids of the pericarp extracts were the 16:0, 18:1, and 18:2 acids. The 16:1 acid that is characteristic for sea buckthorn oil was detected in the elaegnus fruit in insignificant amounts. The class of sterols, both in the free and in the esterified states, was represented by -sitosterol. The main hydrocarbon of the pericarps and seeds was nonacosane.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 17–21, January–February, 1990.