共查询到20条相似文献,搜索用时 10 毫秒
1.
Competitive amperometric morphine sensor based on an agarose immobilised molecularly imprinted polymer 总被引:3,自引:0,他引:3
A morphine-sensitive device was constructed based on a molecularly imprinted polymer. The imprinted polymer exhibited recognition properties previously. A method of detection based on competitive binding was used to measure morphine in the concentration range 0.1–10 μg/ml. A morphine concentration of 0.5 μg/ml gave a peak current (by oxidation) of 4 nA. The method of morphine detection involves two steps. In the first step, morphine binds selectively to the molecularly imprinted polymer in the sensor. In the second step, an electroinactive competitor (codeine) is added in excess, whence some of the bound morphine is released. The released morphine is detected by an amperometric method. The advantages of this type of sensor compared to biosensors based on antibodies, enzymes or cells are discussed. This sensor, based on an artificial recognition system, demonstrates autoclave compatibility, long-time stability and resistance to harsh chemical environments. 相似文献
2.
A novel amperometric sensor based on electropolymerized molecularly imprinted polymer (MIP) for triclosan detection is reported. The sensor was prepared by electropolymerizing o-phenylenediamine (o-PD) on a glassy carbon electrode in the presence of template triclosan. The template can be quickly removed by NaOH solution. After incubating in acetate buffer for 15 min, the sensor response sensitively to triclosan over a linear range of 2.0 × 10− 7 to 3.0 × 10− 6 mol/L and a detection limit as low as 8.0 × 10− 8 mol/L is obtained. This sensor provides an efficient way for eliminating interferences from compounds with similar structures to that of triclosan. 相似文献
3.
4.
5.
6.
A novel potentiometric sensor based on molecularly imprinted polymer (MIP) for propranolol, an adrenergic-blocking drug, was designed. The influence of molecularly imprinted polymer particle content and sodium tetraphenylborate additives in polyvinylchloride membrane was shown. The electrodes show near-Nernstian responses down to 10?4–10?5?M propranolol concentration. The potentiometric response of MIP-based sensor for propranolol in mixed nonaqueous medium was shown at first. Sensor selectivity relative to various inorganic cations, atenolol and metoprolol, was reported. Direct potentiometry was used to determine propranolol in aqueous modeling solutions and pharmaceutical preparations with good results. 相似文献
7.
Song Wei Chen Yu Xu Juan Yang Xiao-Rong Tian Dan-Bi 《Journal of Solid State Electrochemistry》2010,14(10):1909-1914
Molecularly imprinted polymers (MIPs) have been applied as molecular recognition elements to chemical sensors. In this paper,
we combined the use of MIPs and electropolymerization to produce a sensor which was capable of detecting dopamine (DA). The
MIP electrode was obtained by electrocopolymerization of o-phenylenediamine and resorcinol in the presence of the template molecular DA. The MIP electrode exhibited a much higher current
response compared with the non-imprinted electrode. The response of the imprinted sensor to DA was linearly proportional to
its concentration over the range 5.0 × 10−7-4.0 × 10−5 M. The detection limit of DA is 0.13 μM (S/N = 3). Moreover, the proposed method could discriminate between DA and its analogs, such as ascorbic acid and uric acid. This
method was successfully applied to the determination of DA in dopamine hydrochloride injection and healthy human blood serum.
These results revealed that such a sensor fulfilled the selectivity, sensitivity, sped, and simplicity requirements for DA
detection and provided possibilities of clinical application in physiological fields. 相似文献
8.
Houjiang Zhou Deyong He Yufei Hu Ying Huang Dinglong Chen 《Analytica chimica acta》2004,523(2):237-242
In this paper, a novel flow chemiluminescence (CL) clenbuterol sensor based on molecularly imprinted polymer (MIP) on line enrichment nanogram clenbuterol and chemiluminescence reaction of potassium permanganate and formaldehyde in the polyphosphate enhanced by clenbuterol. Clenbuterol in the urine was selectively adsorbed on the clenbuterol-imprinted polymer, which was packed into the flow cell. The formaldehyde and the polyphosphate with potassium permanganate flowed through the flow cell and reacted with the on line adsorbed clenbuterol and produced strong CL. The results show that the sensor was reversible. The CL intensity was linear with clenbuterol concentration from 1.0 × 10−9 g/mL to 5.0 × 10−8 g/mL. The detection limit was 3.0 × 10−10 g/mL. The R.S.D. for ng/mL clenbuterol was less than 5% (n = 3). The present method offered a high selectivity and sensitivity that made the quantitative analysis of trace clenbuterol (ng/mL) in the animal urine sample. 相似文献
9.
采用超声法制备g-C3N4/NiO复合催化材料,将其滴涂在玻碳电极(GCE)表面构建g-C3N4/NiO/GCE催化电极。采用电聚合方法,以马尿酸为模板分子,邻苯二胺为功能单体构建M IP/g-C3N4/NiO/GCE传感器。采用时间-电流法对传感器制备条件优化。结果表明,复合催化材料对H2O2具有良好的催化效果。分子印迹传感器对马尿酸具有良好的选择性。在优化条件下,马尿酸质量浓度在0.05~2 mg/L范围内,电极电流响应与浓度呈良好的线性关系,检出限为7.1μg/L。将电极用于尿液中马尿酸的检测,回收率在87.3%~95.3%之间。 相似文献
10.
三氯生分子印迹传感器的制备及其性能研究 总被引:1,自引:0,他引:1
应用分子印迹技术, 以邻苯二胺为功能单体、三氯生为模板, 用循环伏安法在玻碳电极表面合成了性能稳定的三氯生分子印迹聚合膜, 并用方波伏安法对此印迹传感器进行了分析应用研究. 相似文献
11.
Biomimetic piezoelectric quartz sensor for caffeine based on a molecularly imprinted polymer 总被引:2,自引:0,他引:2
A piezoelectric quartz sensor coated with molecularly imprinted polymer (MIP) for caffeine was developed. The MIP was prepared by co-polymerizing methacrylic acid (MAA) and ethylene glycol dimethacrylate (EDMA) in the presence of azobis(isobutyronitrile) as initiator, caffeine as template molecule, and chloroform as solvent. The MIP suspension in polyvinyl chloride/tetrahydrofuran (6:2:1 w/w/v) solution was spin coated onto the surface of the electrode of a 10 MHz AT-cut quartz crystal. The sensor exhibited a linear relationship between the frequency shift and caffeine concentration in the range of 1×10–7 mg mL–1 up to 1x10–3 mg mL–1 [correlation coefficient (r)=0.9935] in a stopped flow measurement mode. It has a sensitivity of about 24 Hz/ln(concentration, mg mL–1). A steady-state response was achieved in less than 10 min. The performance characteristic of the sensor shows a promising and inexpensive alternative method of detecting caffeine. Surface studies were carried out for the reagent phase of the sensor using SEM, AFM, and XPS analysis in order to elucidate the imprinting of the caffeine molecule. The SEM micrograph, AFM image, and XPS spectra confirmed the removal of caffeine by Soxhlet extraction in the imprinting process and the rebinding of caffeine to the MIP sensing layer during measurement. 相似文献
12.
Surface plasmon resonance sensor for lysozyme based on molecularly imprinted thin films 总被引:1,自引:0,他引:1
Molecularly imprinted polymers (MIPs) selective for lysozyme were prepared on SPR sensor chips by radical co-polymerization with acrylic acid and N,N′-methylenebisacrylamide. Gold-coated SPR sensor chips were modified with N,N′-bis(acryloyl)cystamine, on which MIP thin films were covalently conjugated. The presence of NaCl during the polymerization and the re-binding tests affected the selectivity and the optimization of NaCl concentration in the pre-polymerization mixture and the re-binding buffer could enhance the selectivity in the target protein sensing. When the lysozyme-imprinted polymer thin films were prepared in the presence of 40 mM NaCl, the selectivity factor (target protein bound/reference protein bound) of MIP in the re-binding buffer containing 20 mM NaCl was 9.8, meanwhile, that of MIP in the re-binding buffer without NaCl was 1.2. A combination of SPR sensing technology with protein-imprinted thin films is a promising tool for the construction of selective protein sensors. 相似文献
13.
Chengdu Liang Hui Peng Lihua Nie Shouzhuo Yao 《Analytical and bioanalytical chemistry》2000,367(6):551-555
A novel molecularly imprinted polymer (MIP) coated bulk acoustic wave (BAW) sensor was fabricated for liquid phases. It can be used as a sensitive portable implement for the assay of 2,4-dichlorophenoxyacetic acid. A logarithmic response relationship between 2.0 × 10–7 M and 5.0 × 10–4 M was found. The detection limit was 1.0 × 10–7 M. Recoveries were 93.2–108.7%. Influencing factors were investigated in detail and optimized. 相似文献
14.
Bulk acoustic wave sensor for herbicide assay based on molecularly imprinted polymer 总被引:1,自引:0,他引:1
A novel molecularly imprinted polymer (MIP) coated bulk acoustic wave (BAW) sensor was fabricated for liquid phases. It can be used as a sensitive portable implement for the assay of 2,4-dichlorophenoxyacetic acid. A logarithmic response relationship between 2.0 x 10(-7) M and 5.0 x 10(-4)M was found. The detection limit was 1.0 x 10(-7) M. Recoveries were 93.2-108.7%. Influencing factors were investigated in detail and optimized. 相似文献
15.
Zhaoyang Wu Xiaolei Zhang Yunhui Yang Guoli Shen Ruqin Yu 《Frontiers of Chemistry in China》2006,1(2):183-187
A sensitive nicotine sensor based on a molecularly imprinted electropolymer of o-aminophenol is proposed and its configuration
and performance are studied in detail. On the condition of weak acidity, the sensitive layer was prepared by electropolymerization
of o-aminophenol on a gold electrode in the presence of the template (nicotine). The sensor exhibits good selectivity and
sensitivity to nicotine. The determination limit is 2.0×10−7 mol/L and a linear relationship between the current and concentration is found in the range of 4.0×10−7 ∼ 3.3 ×10−5 mol/L. The sensor has also been applied to the analysis of nicotine in tobacco samples with recovery rates ranging from 99.0%
to 102%.
__________
Translated from Journal of Hunan university (Nature Science), 2005, 32(3) (in Chinese) 相似文献
16.
该文将光子晶体与分子印迹技术结合,制备了分子印迹光子晶体水凝胶膜(MIPHs)作为光学传感器,用于样品中三聚氰胺的快速识别检测。以三聚氰胺为模板分子,通过垂直沉降自组装、填充聚合、去除模板3个步骤,制备得到了反蛋白石结构的三聚氰胺MIPHs。扫描电子显微镜的形貌表征表明,MIPHs具有高度有序的三维大孔结构。该MIPHs作为光学传感器可以将三聚氰胺的分子特异识别过程转换成光学信号,在对目标分析物分析时具有选择性高、响应快、灵敏度高的优点。此外,可以根据MIPHs的颜色变化利用图像软件分析或裸眼识别的方式实现目标分析物快速识别。实验结果表明,在最优条件下,三聚氰胺浓度为10-11~10-6mol/L时,MIPHs的布拉格衍射峰位移从563 nm红移到608 nm,而对三聚氰胺的结构类似物没有明显响应。 相似文献
17.
Leveriza F. Arenas Benilda S. Ebarvia Fortunato B. Sevilla III 《Analytical and bioanalytical chemistry》2010,397(7):3155-3158
A piezoelectric quartz crystal (PQC) sensor based on a molecularly imprinted polymer (MIP) has been developed for enantioselective
and quantitative analysis of d-(+)-methamphetamine (d(+)-MA). The sensor was produced by bulk polymerization and the resulting MIP was then coated on the
gold electrode of an AT-cut quartz crystal. Conditions such as volume of polymer coating, curing time, type of PQC, baseline
solvent, pH, and buffer type were found to affect the sensor response and were therefore optimized. The PQC-MIP gave a stable
response to different concentrations of d(+)-MA standard solutions (response time = 10 to 100 s) with good repeatability (RSD = 0.03
to 3.09%; n = 3), good reproducibility (RSD = 3.55%; n = 5), and good reversibility (RSD = 0.36%; n = 3). The linear range of the sensor covered five orders of magnitude of analyte concentration, ranging from 10−5 to 10−1 μg mL−1, and the limit of detection was calculated as 11.9 pg d(+)-MA mL−1
. The sensor had a highly enantioselective response to d(+)-MA compared with its response to l(−)-MA, racemic MA, and phentermine.
The developed sensor was validated by applying it to human urine samples from drug-free individuals spiked with standard d(+)-MA
and from a confirmed MA user. Use of the standard addition method (SAM) and samples spiked with d(+)-MA at levels ranging
from 1 × 10−3 to 1 × 10−2 μg mL−1 showed recovery was good (95.3 to 110.9%). 相似文献
18.
We have developed a piezoelectric sensor for the determination of atrazine. It is based on the modification of a molecularly imprinted film of TiO2 that was placed on a quartz crystal via a surface sol?Cgel process. The resulting sensor exhibits high selectivity for atrazine, a re-usability that is better than that of other sensors, a response time of 3?min, a wider linear range (0.0005?C8?mM), and a lower detection limit (0.1???M). The analytical application of the atrazine sensor confirms the feasibility of atrazine determination. Graphical abstract
The response of QCM electrodes prepared by various methods to atrazine (pH 5.0). 相似文献
19.
20.
Shan Xueqing de Dieu Habimana Jean Ji Jian Sun Jiadi Pi Fuwei Zhang Yinzhi Sun Xiulan 《Journal of Solid State Electrochemistry》2019,23(4):1211-1220
Journal of Solid State Electrochemistry - A novel electrochemical sensor for paraquat (PQ) detection based on molecularly imprinted polymer (MIP) membranes on a glassy carbon electrode (GCE)... 相似文献