共查询到18条相似文献,搜索用时 531 毫秒
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通过环氧氯丙烷活化(1,4)-2-氨基-2-脱氧β-D-葡萄糖(壳聚糖)制备免疫吸附剂,详细讨论了环氧氯丙烷活化过程的影响因素,确定了活化的最佳条件,活化(1,4)-2-氨基-2-脱氧β-D-葡萄糖树脂对单克隆抗体的偶联率为17.6%,对含乙肝炎表面抗原阳性血清均吸附实验表明,制备的免疫吸附剂剂能使阳性转为阴性,吸附率为44%。 相似文献
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氨基酸—VT对SLE病理性抗体的吸附 总被引:3,自引:1,他引:3
本文研究了用VT树脂偶联各种氨基酸制备的吸附剂对系统性红斑狼疮患者血清中病理性抗体的吸附作用。实验结果表明,多种氨基酸-VT对SLE患者血清中IgG抗体有吸附作用,其中Ser-VT,Gln-VT和Tyr-VT选择性吸附抗-DNA等致病性抗体的效果比较好,同时,提出评价治疗SLE用吸附剂吸除效果的四个指标。 相似文献
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本文在最佳条件下将猪Clq与活化的VT树脂反应制备了Clq-VT免疫吸附剂,每克干重VT可结合0.5mgClq,可吸附1.4mg热凝聚人IgG(AHG)。SDS—PAGE结果表明Clq-VT免疫吸附剂只对AHG特鼻吸附,而不吸附其它血清蛋白。应用Clq-VT免疫吸附剂吸附SLE病人血浆中循环免疫复合物(CIC),结果表明约有52%的CIC被清除。Clq-VT免疫吸附剂只能一次性地使用。 相似文献
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本文在最佳条件下将猪C1q与活化的VT树脂反应制备了C1q-VT免疫吸附剂,每克干重VT可结合0.5mgC1q,可吸附1.4mg热凝聚人IgG(AHG)。SDS-PAGE结果表明C1q-VT免疫吸附剂只对AHG特异吸附,而不吸附其它血清蛋白。应用C1q-VT免疫吸附剂吸附SLE病人血浆中循环免疫复合物(CIC),结果表明约有52%的CIC被清除。C1q-VT免疫吸附剂只能一次性地使用. 相似文献
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吸附型“人工肝”辅助材料的制备及其性能研究:Ⅱ.免疫吸附剂的制?… 总被引:1,自引:0,他引:1
本文介绍以醋酸乙烯酯为单体,二乙烯苯为交联剂制得大孔共聚物,经皂化,活化后,偶联IgG制得免疫吸附剂、讨论了不同活化剂对偶联配体的影响,观察其对人血清中的HBsAg的吸附性能。 相似文献
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球形纤维素固定化DNA制备免疫吸附剂 总被引:15,自引:0,他引:15
以球形纤维素为载体,经环氧氯丙烷活化后共价键联小牛胸腺DNA,制备DNA免疫吸附剂,通过血液灌流能够治疗系统性红斑狼疮.对病人血清的吸附实验结果表明,每毫升吸附剂与3mL病人血清混合,于37℃保温1 h,可吸附除去40%~70%致病抗体 相似文献
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CHEN Shuixia XU Ruimei ZHANG Xiaoping ZENG Hanmin Materials Science Institute Zhongshan University Guangzhou China . Department of Environmental Science Engineering South China University of Technology Guangzhou China 《Chinese Journal of Reactive Polymers》2002,(2)
1. INTRODUCTION The discharge of effluent containing precious metal ions, which comes from electroplating, mining, smelting, and other industries, not only contaminate the environment where people live, but also cause the waste of natural resources. From… 相似文献
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The reduction adsorption of silver diamminonitrate on different kinds of activated carbonfibers (ACF) has been studied in this paper. The effect of different parameters, including adsorptiontemperature, concentrations of activation agents, and activation time on the silver adsorptioncapacities of activated carbon fibers has been investigated The results show that higher temperaturein which the silver complex interacts with ACF. or higher concentration of activation agent, will makehigher reduction adsorption capacities of ACFs. More over, ACFs activated with phosphoric acidhave higher reduction capacities than those activated with zinc chloride or steam. 相似文献
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Waste ion-exchange resin was utilized as precursor to produce activated carbon by KOH chemical activation, on which the effects of different activation temperatures, activation times and impregnation ratios were studied in this paper. The CO2 adsorption of the produced activated carbon was tested by TGA at 30 °C and environment pressure. Furthermore, the effects of preparation parameters on CO2 adsorption were investigated. Experimental results show that the produced activated carbons are microporous carbons, which are suitable for CO2 adsorption. The CO2 adsorption capacity increases firstly and then decreases with the increase of activation temperature, activation time and impregnation rate. The maximum adsorption capacity is 81.24 mg/g under the condition of 30 °C and pure CO2. The results also suggest that waste ion-exchange resin-based activated carbons possess great potential as adsorbents for post-combustion CO2 capture. 相似文献
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A series of activated carbons were prepared from sawdust by zinc chloride activation in different operation conditions. The
effects of operation parameters such as impregnation ratio, activation temperature and time on the adsorption properties of
activated carbons were measured and analyzed in order to optimize these operation conditions. The experimental results show
that under the experimental circumstances studied, both the yield and the adsorption for iodine and methylene blue of activated
carbon can reach a relatively higher value in the chemical activation process with the impregnation ratio of 100% ZnCl2/sawdust in the activation temperature of 500 °C carbonized for 60–90 minutes which are the optimum activation conditions
in making wood activated carbon. The most important operation parameter in chemical activation with zinc chloride was found
to be the impregnation ratio. 相似文献
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Tryptophan residues of IgGRF from rheumatoid arthritis patient were modified with N-Bromosuccinimide(NBS) and the product of modification was characterized by UV-spectrum、Fluorescence emission spectrum and CD-spectrum.The corresponding adsorption capacity of immobilized ssDNA immunoadsorbent for IgGRF was enhanced from 46% to 86%,This result indicated the tryptophan residue is essential for the interaction between ssDNA and IgGRF.and ionic-bonding plays an important role in adsorption. 相似文献
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Summary High-performance liquid chromatography (HPLC) has been used to study the adsorption kinetics of proteins on immunoadsorbents.
The adsorption rate constant of human serum albumin (HSA) on monoclonal and polyclonal anti-HSA antibodies immobilized on
a silica HPLC support was determined by saturating the column with repeated pulse injections. Studies on polyclonal immunodsorbents
of different capacities enable evaluation of the contribution of transport to the binding sites.
The adsorption properties of two different monoclonal anti-HSA antibodies immobilized on a chromatographic support were characterized
by different approaches. The location of the epitope on the HSA molecule was determined by enzyme-linked immunoassay (ELISA)
with albumin fragments. The chromatographic method was used to determine the column capacity and the adsorption rate constant
of HSA on the immunoadsorbent. To compare the affinity of the antibodies for the antigen, an indirect ELISA method was used
to determine the equilibrium constant of antigen-antibody association in solution
Presented at the 21st ISC held in Stuttgart, Germany, 15th–20th september, 1996. 相似文献