首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 15 毫秒
1.
    
Laboratory experiments are useful for estimating the carbon and nitrogen isotope discrimination factors and turnover rates that are critical for drawing field-based inferences on consumer diets using stable isotopes. Although the utility of these discrimination factors is widely recognized, work in terrestrial systems has largely been limited to studies involving mammals and birds. In contrast, scant attention has been paid to the application of isotopic techniques to reptiles, despite their broad diversity in terms of numbers of species as well as their trophic roles. Here we estimate carbon and nitrogen isotope discrimination factors and turnover for the tree lizard (Urosaurus ornatus) using a diet-switch experiment. Lizards were collected from a C4-dominated grassland and then switched to C3-based diet (crickets) in the laboratory. We estimated discrimination by lizard claw tissue as Δ13C?=?1.2?±?0.1?‰ for carbon and Δ15N?=?0.7?±?0.1?‰ (mean?±?1 SE) for nitrogen, with 95?% turnover occurring after ~15.5 days. These estimates should be appropriate for use in trophic studies of U. ornatus, and possibly other related small-bodied insectivorous lizards.  相似文献   

2.
Western France is at the crossroads of the migratory routes of two subspecies of black-tailed godwit, Limosa limosa. After leaving Iceland, the godwit L.l. islandica Icelandic black-tailed godwit (IBTG) winters on the coast of western Europe, while the continental black-tailed godwit (CBTG) L.l. limosa can stop in France when migrating between Iberia or Africa and their main breeding grounds in the Netherlands. In this study, we analysed δ15N and δ13C from flight feathers and whole blood throughout the non-breeding period to trace variations in habitat use for both subspecies on the western French coast. Adults and juveniles of IBTG adopt the same feeding habitats as soon as they arrive in the study area, progressively losing the Icelandic freshwater habitat signal, and becoming clearly restricted to marine habitats in winter. Some individuals begin to move locally to freshwater habitats, joining CBTG in a stopover at the end of the wintering period in preparation for northward migration.  相似文献   

3.
    
We present the results of a study testing the response of two saprophytic white-rot fungi species, Pleurotus pulmonarius and Coriolus versicolor, to charred biomass (charcoal) as a growth substrate. We used a combination of optical microscopy, scanning electron microscopy, elemental abundance measurements, and isotope ratio mass spectrometry (13C and 15N) to investigate fungal colonisation of control and incubated samples of Scots Pine (Pinus sylvestris) wood, and charcoal from the same species produced at 300 °C and 400 °C. Both species of fungi colonise the surface and interior of wood and charcoals over time periods of less than 70 days; however, distinctly different growth forms are evident between the exterior and interior of the charcoal substrate, with hyphal penetration concentrated along lines of structural weakness. Although the fungi were able to degrade and metabolise the pine wood, charcoal does not form a readily available source of fungal nutrients at least for these species under the conditions used in this study.  相似文献   

4.
    
We used stable isotope ratios to determine the metabolic routing fraction of carbon and nitrogen in feathers in addition to faecal analysis to estimate diet overlap of six sympatric species of manakins in the eastern lowland forest of Ecuador. Collectively, all species varied from?23.7 to?32.7 ‰ for δ13C, and from 6.0 to 9.9‰ for δ15N, with Machaeropterus regulus showing isotopic differences from the other species. We developed a mixing model that explicitly addresses the routing of carbon and nitrogen to feathers. Interestingly, these results suggest a higher proportion of nitrogen and carbon derived from insects than anticipated based on feeding observations and faecal analysis. A concentration-dependent mixing isotopic model was also used to look at dietary proportions. While larvae and arachnids had higher δ15N values, these two groups may also be preferred prey of manakins and may be more assimilated into tissues, leading to a potential overestimation of the contribution to diet. This study supports the finding that manakin species, previously thought be primarily frugivorous, contain a significant amount of arthropods in their diet.  相似文献   

5.
    
Overgrazing often lowers species richness and productivity of grassland communities. For Mongolian grassland ecosystems, a lack of detailed information about food-web structures makes it difficult to predict the effects of overgrazing on species diversity and community composition. We analysed the δ13C and δ15N signatures of herbaceous plants, arthropods (grouped by feeding habit), wild and domestic mammals, and humans in central Mongolia to understand the predominant food-web pathways in this grassland ecosystem. The δ13C and δ15N values of mammals showed little variation within species, but varied considerably with slope position for arthropods. The apparent isotopic discrimination between body tissue and hair of mammals was estimated as 2.0 ‰ for δ13C and 2.1 ‰ for δ15N, which was large enough to cause overestimation of the trophic level of mammals if not taken into account when using hair samples to measure isotopic enrichment.  相似文献   

6.
    
Stable isotope analysis was applied to describe the poultry house environment. The poultry house indoor environment was selected for this study due to the relevant health problems in animals and their caretakers. Air quality parameters including temperature, relative humidity, airflow rate, NH3, CO2 and total suspended particles, as well as mean levels of total airborne bacteria and fungi count, were measured. Carbon isotope ratios (13C/12C) were obtained in size-segregated aerosol particles. The carbon (13C/12C) and nitrogen (15N/14N) isotope ratios were measured in feed, litter, scrapings from the ventilation system, feathers and eggs. Additionally, the distribution of δ13C and δ15N values in different tissues of the chicken was examined. The airborne bacteria and fungi extracted from the air filters collected from poultry farms were grown in the laboratory in media with known isotope values and measured for stable isotope ratios. Analysis of isotope fractionation between microorganisms and their media indicated the applicability of stable isotope analysis in bulk samples for the identification of source material. The analysed examples imply that stable isotope analysis can be used to examine the indoor environment along with its biology and ecology, and serve as an informative bioanalytical tool.  相似文献   

7.
Variations in the relative isotopic abundance of C and N (δ13C and δ15N) were measured during the composting of different agricultural wastes using bench-scale bioreactors. Different mixtures of agricultural wastes (horse bedding manure?+?legume residues; dairy manure?+?jatropha mill cake; dairy manure?+?sugarcane residues; dairy manure alone) were used for aerobic–thermophilic composting. No significant differences were found between the δ13C values of the feedstock and the final compost, except for dairy manure?+?sugarcane residues (from initial ratio of ?13.6?±?0.2?‰ to final ratio of ?14.4?±?0.2?‰). δ15N values increased significantly in composts of horse bedding manure?+?legumes residues (from initial ratio of +5.9?±?0.1?‰ to final ratio of +8.2?±?0.5?‰) and dairy manure?+?jatropha mill cake (from initial ratio of +9.5?±?0.2?‰ to final ratio of +12.8?±?0.7?‰) and was related to the total N loss (mass balance). δ13C can be used to differentiate composts from different feedstock (e.g. C3 or C4 sources). The quantitative relationship between N loss and δ15N variation should be determined.  相似文献   

8.
2H/1H ratios in animal biomass reflect isotopic input from food and water. A 10-week controlled laboratory study raised 48 mice divided in two generations (8 mothers Mus musculus and their offspring). The mice were divided into four groups based on the combination of 2H, 13C, 15N-enriched and non-enriched food and water. Glycine, the most common amino acid in bone collagen, carried the 2H, 13C, 15N-isotopic spike in food. ANOVA data analysis indicated that hydrogen in food accounted for ~81?% of the hydrogen isotope inventory in collagen whereas drinking water hydrogen contributed ~17?%. Air humidity contributed an unspecified amount. Additionally, we monitored 13C and 15N-enrichment in bone collagen and found strong linear correlations with the 2H-enrichment. The experiments with food and water indicate two biosynthetic pathways, namely (i) de novo creation of non-essential amino acids using hydrogen from water, and (ii) the integration of essential and non-essential amino acids from food. The lower rate of isotope uptake in mothers’ collagen relative to their offspring indicates incomplete bone collagen turnover after ten weeks. The variance of hydrogen stable isotope ratios within the same cohort may limit its usefulness as a single sample proxy for archaeological or palaeoenvironmental research.  相似文献   

9.
Nile crocodiles undergo a three to five order of magnitude increase in body size during their lifespan. This shift coincides with a change in resource and habitat use which influences the strength, type and symmetry of interactions with other species. Identifying size-specific crocodile groups displaying similar traits is important for conservation planning. Here, we illustrate how stable carbon (δ13 C) and nitrogen (δ15 N) isotope analysis of scute keratin, together with breakpoint modelling analysis can be used to characterise ontogenetic niche shifts. Using a sample set of 238 crocodiles from the Okavango Delta, Botswana (35–463 cm total length), we found prominent size-related changes in the scute keratin δ13 C and δ15 N profiles close to 40 and 119 cm snout-vent length. The first shift corroborated the findings of a traditional stomach-content study conducted on the same population at the same time, and the second conformed to known crocodile ecology. This approach can be used as a first approximation to identify size-specific groups within crocodile populations, and these can then be investigated further using isotopic or other methods.  相似文献   

10.
11.
    
The use of nitrogen stable isotopes to discriminate between conventionally and organically grown crops has been further developed in this study. Soil and irrigation water from different regions, as well as nitrogen fertilizers used, have been examined in detail to determine their effects on nitrogen isotope composition of spinach, lettuce, broccoli and tomatoes. Over 1000 samples of various types of organically and conventionally grown produce of known origin, along with the samples of nitrogen fertilizers used for their growth, have been analysed in order to assemble the datasets of crop/fertilizer correlations. The results demonstrate that the developed approach can be used as a valuable component in the verification of agricultural practices for more than 25 different types of commercially grown green produce, either organic or conventional. Over a period of two years, various organic and non-organic greens, from different stores in Seattle (WA, USA) and Victoria (BC, Canada), were collected and analysed using this methodology with the objective of determining any pattern of misrepresentation.  相似文献   

12.
Diet–tissue isotopic relationships established under controlled conditions are informative for determining the dietary sources and geographic provenance of organisms. We analysed δ13C, δ15N, and non-exchangeable δ2H values of captive African grey parrot Psittacus erithacus feathers grown on a fixed mixed-diet and borehole water. Diet–feather Δ13C and Δ15N discrimination values were +3.8?±?0.3?‰ and +6.3?±?0.7?‰ respectively; significantly greater than expected. Non-exchangeable δ2H feather values (?62.4?±?6.4?‰) were more negative than water (?26.1?±?2.5?‰) offered during feather growth. There was no positive relationship between the δ13C and δ15N values of the samples along each feather with the associated samples of food offered, or the feather non-exchangeable hydrogen isotope values with δ2H values of water, emphasising the complex processes involved in carbohydrate, protein, and income water routing to feather growth. Understanding the isotopic relationship between diet and feathers may provide greater clarity in the use of stable isotopes in feathers as a tool in determining origins of captive and wild-caught African grey parrots, a species that is widespread in aviculture and faces significant threats to wild populations. We suggest that these isotopic results, determined even in controlled laboratory conditions, be used with caution.  相似文献   

13.
    
The preferred tissue for analyses of fish stable isotope ratios for most researchers is muscle, the sampling of which typically requires the specimen to be sacrificed. The use of non-destructive methods in fish isotopic research has been increasing recently, but as yet is not a standard procedure. Previous studies have reported varying levels of success regarding the utility of non-lethally obtained stable isotope materials, e.g. fins, but none have accounted for the potential compounding effects of inorganic components of fin rays or lipids. Comparisons of carbon (δ13C) and nitrogen (δ15N) stable isotope ratios of muscle with adipose and caudal fin of two salmonids, Atlantic salmon (Salmo salar L.) and brown trout (Salmo trutta L.), revealed that caudal fin can be used as a non-destructive surrogate for muscle in stable isotope analysis, but that adipose fin, where available, is a better proxy. The use of a published model to inexpensively counteract the confounding effect of lipids, which are depleted in 13C, greatly improved the relationship between fish muscle and fins. However, efforts to account for the inorganic components of fin rays were counterproductive and required twice the biomass of fins clipped from each fish. As this experiment was conducted on wild fish, controlled laboratory studies are required to confirm these field observations.  相似文献   

14.
The spatial variations in the elemental and stable carbon, nitrogen, and sulphur isotope composition of bladder wrack (Fucus vesiculosus) growing along the shore line of the semi-enclosed urbanized Kiel Fjord (western Baltic Sea) was investigated at more than 60 sites. The analyses of the carbon-nitrogen-sulphur (CNS) stoichiometry and C and N stable isotope signature of F. vesiculosus displayed substantial differences between the north-western and the south-eastern parts of the Kiel Fjord. Different size classes displayed in part differences in C:N and C:S ratios, and the carbon isotope composition, reflecting the impact of the boundary conditions during growth. Whereas the sulphur isotope composition was controlled by the assimilation of seawater sulphate, the carbon isotope composition reflected the difference in the composition of surface waters. The δ15N values of the organic tissue tend to be an integrated monitor of anthropogenic impacts on the fjord. Results are compared to the composition of surface waters.  相似文献   

15.
Based on the assumptions that human food is available for dogs and isotope diet–tissue differences are similar in dogs and humans, the ‘canine surrogacy approach’ (CSA) has been used to infer patterns of ancient populations. The goal of this study was to test the CSA in urban (Brasília and Piracicaba) and in rural (Ubatuba and Maraã) areas. The hair C and N isotope ratios of modern dogs were compared with those of human fingernails from different regions of Brazil. Our CSA results showed a correlation between dog and human isotopes values: in rural areas δ15N of humans and dogs was not statistically different; contrarily, in urban centres, δ15N of humans was approximately 1?‰ higher (p?<?0.01) than δ15N of dogs; humans had lower δ13C values (p?<?0.01) than dogs in Brasília, Piracicaba and Ubatuba. In Maraã, there was not any significant difference between dogs and humans. We concluded that CSA is still valid as a first approach in modern societies. However, isotopic differences found suggest that in modern societies processed dog food is increasingly disconnecting human and dog, jeopardising the use of CSA in the future if the trend of increasing processed dog food consumption continues to occur.  相似文献   

16.
    
The transformation from self-supporting lianas to host-supported climbing lianas is related to re-allocation of biomass and nutrients among plant organs. Therefore, first, variations in leaf mass per area (LMA), leaf carbon and nitrogen allocation and 13C and 15N natural abundances were analysed among three tropical Passiflora species (P. edulis, P. ligularis, and P. tripartita) in a greenhouse study. Second, the influence of a climbing support was considered for each species and parameter. P. ligularis leaves were most enriched in 13C in both treatments when compared with the other two species. This enrichment was caused by a high LMA, which is related to a high internal resistance to CO2 diffusion. For P. edulis and P. tripartita, δ13C was additionally increasing with nitrogen content per area. Generally, there were no differences when considering carbon and nitrogen allocation to leaves of host-supported and self-supporting lianas. The only hints towards increased investment into leaves after the transition from self-supporting to host-supported stages could be seen by a trend to increased leaf areas and masses. δ13C values of supported P. edulis or P. tripartita plants were significantly increasing faster than those of non-supported plants once the interactions of leaf mass or nitrogen content per area were accounted for. Hence, the offer of a climbing support had only a minor impact on δ13C or δ15N values in vitro, but this could be different with increasing age of lianas in vivo.  相似文献   

17.
    
Nitrogen (N) retention and transformation in the Szczecin Lagoon, southern Baltic Sea, were studied by means of budget calculations and stable isotope data of dissolved and particulate matter. Two stations, one located at the main outlet of the lagoon (?wina Strait) and the other 100 km to the south, on the Oder River (Widuchowa), were sampled biweekly over the years 2000–2002. The Oder River is one of the five largest rivers draining into the Baltic Sea and the largest one discharging its waters into the western Baltic. According to our data, the Oder River carried approximately 60 kt y?1 total N, of which 7 kt y?1 (<12 %) are particulate organic nitrogen and 46 kt y?1 (77 %) dissolved inorganic nitrogen. Seasonal patterns of particulate nitrogen and nitrate concentrations were similar at Widuchowa and ?wina Strait station, but nitrate concentrations in the ?wina Strait were much lower, pointing not only to the dilution effect but also to considerable nutrient removal capacity (especially of nitrate) in the lagoon. The loss of nitrate suggests that denitrification is the major N-removal process, whereas primary production was only a minor contributor, due to the very low particle load. Combining budget calculations with stable isotope measurements reveal unique information about nitrogen turnover processes in the lagoon.  相似文献   

18.
Po is the main Italian river and the δ18O and δ2H of its water reveal a similarity between the current meteoric fingerprint and that of the past represented by groundwater. As concerns the hydrochemisty, the Ca–HCO3 facies remained constant over the last 50 year, and only nitrate significantly increased from less than 1?mg/L to more than 10?mg/L in the 1980s, and then attenuated to a value of 9?mg/L. Coherently, δ13CDIC and δ34SSO4 are compatible with the weathering of the lithologies outcropping in the basin, while extremely variable δ15NNO3 indicates contribution from pollutants released by urban, agricultural and zootechnical activities. This suggests that although the origin of the main constituents of the Po river water is geogenic, anthropogenic contributions are locally significant. Noteworthy, the associated aquifers have the same nitrogen isotopic signature of the Po river, but are characterized by significantly higher NO 3 concentration. This implies that aquifers’ pollution is not ascribed to inflow of current river water, and that the attenuation of the nitrogen load recorded in the river is not occurring in the aquifers, due to their longer water residence time and delayed recovery from anthropogenic contamination.  相似文献   

19.
Increasing demand for fish and seafood calls for an expansion of aquaculture production. At the same time, the status of the marine environment must not be jeopardised. Stable isotopes are potential markers for tracking feed-based nutrient flows from aquaculture into marine biota. Here, we demonstrate how four experimental diets (main protein components: fishmeal, soya protein concentrate, wheat gluten, and Jatropha kernel meal) and a commercial diet induce characteristic δ13C and δ15N signals in sea bass (Dicentrarchus labrax) during nine weeks of laboratory feeding under replicate conditions. The plant-protein-based diets containing wheat gluten and soya, and the commercial feed consistently induced the largest isotopic differentiation of the fish, both from the feed source and from the pre-experimental condition. The large difference of the fish on plant-protein-based diets compared to the range of natural isotopic variation in the macrozoobenthos of the North Sea lends support to the idea that plant-based feeds are suitable for tracing mariculture-derived organic matter under practical conditions. The commercial feed had a similar effect as the experimental feeds and would be a cost-effective option for an offshore aquaculture experiment.  相似文献   

20.
    
Soil from Free-Air Carbon dioxide Enrichment (FACE) plots (FAL, Braunschweig) under ambient air (375 ppm; δ13C–CO2?9.8‰) and elevated CO2 (550 ppm; for six years; δ13C–CO2?23‰), either under 100% nitrogen (N) (180 kg ha?1) or 50% N (90 kg ha?1) fertilisation treatments, was analysed by thermogravimetry. Soil samples were heated up to the respective temperatures and the remaining soil was analysed for δ13C and δ15N by Isotope Ratio Mass Spectrometry (IRMS). Based on differential weight losses, four temperature intervals were distinguished. Weight losses in the temperature range 20–200 °C were connected mostly with water volatilisation. The maximum weight losses and carbon (C) content were measured in the soil organic matter (SOM) pool decomposed at 200–360 °C. The largest amount of N was detected in SOM pools decomposed at 200–360 °C and 360–500 °C. In all temperature ranges, the δ13C values of SOM pools were significantly more negative under elevated CO2 versus ambient CO2. The incorporation of new C into SOM pools was not inversely proportional to its thermal stability. 50% N fertilisation treatment gained higher C exchange under elevated CO2 in the thermally labile SOM pool (200–360 °C), whereas 100% N treatment induced higher C turnover in the thermally stable SOM pools (360–500 °C, 500–1000 °C). Mean Residence Time of SOM under 100% N and 50% N fertilisation showed no dependence between SOM pools isolated by increasing temperature of heating and the renovation of organic C in those SOM pools. Thus, the separation of SOM based on its thermal stability was not sufficient to reveal pools with contrasting turnover rates of C.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号