L-酪氨酸印迹分子的制备及性能研究

利用分子印迹技术采用传统加热法制备出酪氨酸他子印迹聚合物。用红外光谱分析了聚合物结构。研究了印迹他子与功能单体的物质的量对聚合物结合性的影响,吸收效率表征结果显示,与化学组成相同的空白聚合物相比,印迹聚合物具有更高的吸附效率。     

表面分子印迹研究进展

表面分子印迹聚合物近年来已在手性分离、固相萃取以及化学仿生传感器等领域展现出良好的应用前景,引起了研究者们的广泛关注。表面分子印迹技术是将分子识别位点设计在载体表面或接近表面的地方,制得的表面分子印迹聚合物与传统的分子印迹聚合物相比,具有目标分子更易接近印迹位点、吸附动力学更快、吸附容量更高等优点。本文简述了表面分子印迹研究的最新进展,根据载体种类及表面修饰方法的不同,分别介绍了以硅材料、磁性材料、碳纳米管、石墨烯等为载体的表面分子印迹技术。     

氧化石墨烯基异丙隆分子印迹聚合物的制备及其吸附性能研究

以氧化石墨烯为载体,异丙隆为模板分子,采用表面印迹技术制备了分子印迹聚合物。采用透射电子显微镜、拉曼光谱和热重分析仪对该分子印迹聚合物的结构进行了表征,并通过动态平衡结合法研究了该分子印迹聚合物的吸附能力。结果表明:准二级动力学模型很好地拟合了吸附动力学,相关系数(R2)为0.999 7;与非分子印迹聚合物相比,制备的印迹材料表现出高吸附效率和快速的吸附动力学;选择性吸附试验表明该分子印迹聚合物对异丙隆具有选择性和特异性吸附。     

分子印迹膜的制备研究进展

将分子印迹技术与膜分离技术相结合的分子印迹膜,由于其高选择性,近年来受到了国内外研究者的广泛关注.本文初步探讨了分子印迹膜(MIM) 的两种分离机理以及目前主要的制备方法:同步法和复合法.     

分子印迹聚合物用于环境内分泌干扰物的检测与去除

环境内分泌干扰物对人体健康和生态环境具有严重危害,发展高效的环境内分泌干扰物检测方法与去除技术具有重要意义。由于拥有高度的吸附选择性、较大的吸附容量和良好的可重复利用性,分子印迹聚合物在环境内分泌干扰物的检测与去除方面得到了诸多应用。本文介绍了分子印迹聚合物的制备方法及性能特点,综述了近年来基于分子印迹聚合物的固相萃取技术和传感器技术在环境内分泌干扰物的灵敏、特异性检测中的应用,以及基于分子印迹聚合物的吸附技术与其他方法的联用技术用于环境内分泌干扰物的选择性高效去除,并分析了分子印迹聚合物在合成和使用方面存在的问题,展望了其应用前景。     

松香基氯霉素分子印迹聚合物的制备及其性能研究

通过分子印迹技术以氯霉素为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯和松香交联剂为混合交联剂,采用沉淀聚合法制备了一种松香基氯霉素分子印迹聚合物。通过傅里叶变换红外光谱,扫描电子显微镜,热重分析仪对其进行了表征,并研究了静态吸附和选择性吸附性能。实验结果表明：分子印迹聚合物为粒径均匀的球体,且表面具有丰富的孔隙结构。氯霉素在分子印迹聚合物上的吸附符合拟二级吸附和Langmuir-Freundlich等温吸附模型。在40℃时,分子印迹聚合物对氯霉素的最大吸附量约为72.29 mg/g。同时在25℃,C₀为1.0 mg/mL时,松香基氯霉素分子印迹聚合物印迹因子为2,具有良好的特异性。     

TNT分子印迹聚合物微球的合成与性能研究

以三硝基甲苯(TNT)为模板分子,EDMA为交联剂,采用沉淀聚合法制备了TNT分子印迹微球.讨论了溶剂用量、模板分子用量、功能单体种类等对分子印迹微球的形貌及吸附性能的影响;利用紫外吸收光谱和BET表征了印迹聚合物微球的结合位点相互作用与印迹孔穴结构;通过平衡吸附和选择性吸附实验,研究了印迹聚合物微球的吸附性能和选择性识别性能.结果表明,以丙烯酰胺为功能单体制备的分子印迹聚合物为规则的球形,内部含有分子印迹孔穴,微球的粒径为1～2μm.印迹聚合物微球可在30 min内达到吸附平衡,在1 mmol/L的TNT乙醇溶液中,印迹聚合物微球的平衡吸附量为32.5 mmol/kg,对TNT分离系数为25.19,具有较好的特异性吸附能力,并可选择性识别TNT分子.     

内分泌干扰物雌酮分子印迹新型吸附功能材料的制备及其选择性能研究料

采用分子印迹本体聚合法,制备了对内分泌干扰物雌酮具有高选择识别能力的分子印迹聚合物。吸附动力学和选择性实验结果表明,与非印迹聚合物相比,印迹聚合物具有较高的吸附容量和吸附速率,对模板分子具有较高的选择性。聚合反应条件对印迹聚合物的吸附和识别性能有重要影响,以丙烯酰胺为功能单体,模板分子、功能单体和交联剂摩尔比为1：3：6,制备的印迹聚合物具有较高的选择和吸附性能。     

内分泌干扰物雌酮分子印迹新型吸附功能材料的制备及其选择性能研究

采用分子印迹本体聚合法,制备了对内分泌干扰物雌酮具有高选择识别能力的分子印迹聚合物。吸附动力学和选择性实验结果表明,与非印迹聚合物相比,印迹聚合物具有较高的吸附容量和吸附速率,对模板分子具有较高的选择性。聚合反应条件对印迹聚合物的吸附和识别性能有重要影响,以丙烯酰胺为功能单体,模板分子、功能单体和交联剂摩尔比为1:3:6,制备的印迹聚合物具有较高的选择和吸附性能。     

不同功能单体合成的谷胱甘肽分子印迹聚合物的研究

采用分子印迹技术, 以谷胱甘肽为模板分子, 以丙烯酰胺、甲基丙烯酸为功能单体制备分子印迹聚合物; 通过静态吸附试验, 探讨了合成分子印迹聚合物时模板分子与功能单体的物质的量比、上样液pH 值、吸附平衡时间、上样液浓度对聚合物吸附性能的影响; 实验结果表明: 以丙烯酰胺、甲基丙烯酸为功能单体制备谷胱甘肽分子印迹聚合物时,谷胱甘肽与丙烯酰胺、甲基丙烯酸的最适摩尔比分别为1:5 和1:4, 以及最适静态吸附条件为: 上样液pH 值分别为3.0 和5.0 左右; 上样液浓度分别为1.50～2.00 g/L 和1.00～1.50 g/L; 静态吸附平衡时间为18 和20 h 左右. 同时也探讨了分子印迹聚合物对谷胱甘肽结构类似物的吸附性能, 结果表明, 所合成的分子印迹聚合物对谷胱甘肽具有良好的选择性吸附能力. 同时也研究了分子印迹聚合物对酵母抽提物中谷胱甘肽的吸附性能, 以丙烯酰胺和甲基丙烯酸为功能单体制备的分子印迹聚合物对混合体系中谷胱甘肽的一次性提取率分别为41%和77%. 分子印迹聚合物均表现出了较好的吸附特性, 为分离提纯谷胱甘肽提供一种可选择的途径.     

STUDY ON ASPIRIN-MAA HYDROGEL MIP SYSTEM

The controlled release system of MIP-Asp hydrogel was prepared by using self-assembly molecular imprinted technique （MIP）. ^1H NMR was used to detect the changes of active hydrogen atoms. Moreover, the effect of crosslinking degree of hydrogel on the release of medicine was investigated and the temperature/pH sensitivity was also considered. The results demonstrated that： the MIPs-Asp was a procedure involving the participation of active hydrogen; the lower crosslinking degree corresponded to the higher medicine release ratio; high temperature is better for the release; weak acidity is better for the release of Asp.     

Molecularly imprinted polymers for selective analysis of chemical warfare surrogate and nuclear signature compounds in complex matrices

This paper describes the preparation and evaluation of molecularly imprinted polymers (MIPs) that display specificity toward diisopropyl methylphosphonate (DIMP) and tributyl phosphate (TBP). Polymer activity was assessed by solid-phase extraction and high-performance liquid chromatography experiments. Both DIMP- and TBP-specific vinylpyridine-based MIPs selectively retained their targets relative to a non-imprinted control. Proof-of-principle experiments demonstrated highly selective analysis of the targets from fortified complex matrix samples (diesel fuel, gasoline, and air extract concentrate). The retained MIP fractions gave near quantitative recovery of the target analytes with very low matrix background content. The same fraction from the control sorbent recovered only about half of the analyte and tended to be less pure.     

紫外光聚合法制备L-DBTA手性分子印迹聚合物的研究Ⅰ.紫外光聚合制备条件

以丙烯酸为功能单体,二苯甲酰-L-酒石酸(L-DBTA)为模板分子,三羟甲基丙烷三丙烯酸酯(TMPTA)为交联剂,采用光聚合方法合成了L-DBTA手性分子印迹聚合物,讨论了功能单体种类、功能单体用量、交联剂用量、引发剂用量、三乙胺用量、光聚合温度、光聚合时间、光强度等对L-DBTA手性分子印迹聚合物合成的影响。通过L-DBTA手性分子印迹聚合物对底物的结合实验分析,表明手性分子印迹聚合物对L-DBTA具有很好的识别性,L-DBTA的选择性比二苯甲酰-D-酒石酸(D-DBTA)高,其分离因子可达5．41。     

Determination of histamine in canned tuna by molecularly imprinted polymers-surface enhanced Raman spectroscopy

We introduce a rapid, cost effective and reliable approach to determine histamine level in canned tuna. Molecularly imprinted polymers (MIPs) were synthesized as artificial antibodies towards histamine by utilizing the interaction between histamine and a functional monomer (methacrylic acid) to impress specific binding sites on polymer particles after polymerization. Polyvinyl chloride (PVC) was used to immobilize the MIPs, yielding a MIPs-PVC film that functioned as a recognition element to specifically separate histamine from tuna extract. A gold colloid solution served both as an eluting solvent to extract histamine from MIPs-PVC film and furnish a substrate for surface enhanced Raman spectroscopy (SERS) detection of histamine signals. Principal component analysis together with a partial least square regression (PLSR) model (R² = 0.947, RMSECV = 3.526) verified the reliability of MIPs-PVC-SERS approach for the detection and spectral analysis of histamine. Linear regression models were also constructed to relate the intensity of different histamine SERS bands with the corresponding spiking levels. One such model (using a band at 1576 cm⁻¹) performed slightly better in predicting histamine content in tuna than the PLSR model. We conclude that our new MIPs-PVC-SERS approach can rapidly and reliably determine histamine at levels from 3 to 90 ppm in canned tuna meat.     

分子印迹聚合物传感器研究

分子印迹聚合物(molecular imprinting polymers,MIPs)是利用分子印迹技术合成的一种交联高聚物.分子印迹技术(molecular imprinting technique,MIT)是在近十几年来才发展起来的一门边缘科学技术.它结合了高分子化学、生物化学等学科,是模拟抗体-抗原相互作用的一种新技术,具有选择性识别位点的性质,作为传感器的理想敏感材料的制备方法日益受到研究者们的重视.本文综述了分子印迹技术的原理和分子印迹聚合物的制备方法,及其应用于传感器敏感材料的研究现状,并展望了其发展前景.     

Molecularly Imprinted Electrochemical Sensors

In this review, the applications of molecularly imprinted polymer (MIP) materials in the area of electrochemical sensors have been explored. The designs of the MIPs containing different polymers, their preparation and their immobilization on the transducer surface have been discussed. Further, the employment of various transducers containing the MIPs based on different electrochemical techniques for determining analytes has been assessed. In addition, the general protocols for getting the electrochemical signal based on the binding ability of analyte with the MIPs have been given. The review ends with describing scope and limitations of the above electrochemical based MIP sensors.     

A novel molecular imprinting polymer for the selective adsorption of D-arabinitol from spiked urine

In this research, molecular imprinting polymers (MIPs) for D-arabinitol were synthesized using a bulk polymerization method through a noncovalent approach. The MIPs were prepared by using D-arabinitol as a template, acrylamide as a functional monomer, ethylene glycol dimethacrylateas cross-linker, benzoyl peroxide as an initiator and dimethyl sulfoxideas a porogen. MIPS was synthesized in several formulas with a different molar ratio of template to functional monomers and cross-linker. Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) were used to characterize the MIPs produced. A batch rebinding assay was used to test the binding efficiency of each formula. Batch rebinding test results revealed that MIPsF3 with a molar ratio of the template: monomer and crosslinker ratio respectively (1: 4: 25) had the highest binding capacity at 1.56 mgg ^-1 . The results of isotherm adsorption showed that the MIPs produced followed the Freundlich equation with an R-value of 0.97. The MIPs produced was also selective toward its isomeric compounds (i.e. L-arabinitol, adonitol, xylitol, and glucose). The extraction efficiency of the MIPs against D-arabinitol was 88.98%.     

A new molecularly imprinted polymer for the on-column solid-phase extraction of diethylstilbestrol from aqueous samples

The estrogenic compound diethylstilbestrol (DES) is widely studied because of its potential endocrine disruption effects. The prohibition of the use of diethylstilbestrol as a growth promoter has not been enough to ensure the total disappearance of this compound from environmental matrices. Due to the low levels of DES present in the environment, preconcentration and clean up methods are necessary for its analysis. This paper describes the synthesis and use of a molecularly imprinted polymer (MIP) as sorbent for on-column solid-phase extraction of DES from aqueous samples. The selectivity of the DES-MIP was evaluated towards several selected estrogens such as hexestrol (HEX), estrone (E1), estriol (E3), estradiol (E2) and ethynylestradiol (EE2). HPLC-DAD was used to quantify all analytes at 230-nm wavelength. The method has been successfully applied to the analysis of DES in spiked river and tap water samples, with recoveries of 72% and 83% respectively.     

基于硅材料的分子印迹聚合物的制备及应用*

分子印迹聚合物因制备简单、稳定性好、且具有分子识别功能使其在色谱分离、固相萃取、化学传感、模拟酶催化等方面有了广泛的应用。近年来,基于硅的分子印迹聚合物发展较为快速。本文主要介绍了以硅为母体材料和以硅为基质材料的分子印迹聚合物的制备及分子识别性质研究,并对其应用进行了分类,在此基础上对其将来的发展进行了展望。     

石墨烯修饰电极分子印迹电化学传感器的研制及其对多巴胺的测定

以石墨烯为电极增敏材料,多巴胺印迹聚合物为特异性识别材料,采用滴涂法组装石墨烯修饰电极的分子印迹电化学传感器。考察了pH值、石墨烯浓度、印迹聚合物浓度对传感器的影响,优化的实验条件为:pH 7.0,石墨烯浓度为0.5g/L,印迹聚合物浓度为20g/L。实验表明,该印迹传感器对多巴胺的响应电流远大于非印迹电极,同时该印迹传感器对多巴胺具有较好的选择性,检测范围为2.0×10-7～1.0×10-4mol/L,检出限(S/N=3)为6.8×10-8mol/L。该传感器用于盐酸多巴胺注射液的测定,其回收率为98%～105%。