免疫分析技术在农药残留分析中的研究进展及在中药中的应用展望

中药材的农药残留问题一直是人们关注的重点,免疫分析技术作为一项特异性强、灵敏度高的快速分析检测技术,在实现中药材农药残留快速筛查方面发挥着重要作用.该文结合近年免疫分析相关研究进展,对不同的免疫分析技术在中药农药残留分析方面的应用、技术优势以及局限性进行总结分析,并对免疫分析技术的发展前景进行了展望,对免疫分析技术在农...     

免疫胶体金技术的应用与展望

免疫胶体金技术是一种新型固相标记免疫检测技术,因其独特的性质在许多领域得到广泛应用和快速发展。简述了免疫胶体金技术在电镜、光镜快速检测诊断中的发展以及在生物医学、免疫组织化学和细胞生物学等领域的研究和应用。未来免疫胶体金技术在提高胶体金制备质量的同时将实现定量和多元化检测。     

伏安酶联免疫分析法及其在植物血清学检测技术中的应用

伏安酶联免疫分析法是将免疫技术、酶催化反应与伏安法检测相结合的一种免疫分析新方法。着重探讨了伏安酶联免疫分析法及其在植物血清学检测技术中的应用。     

新型荧光光纤免疫磁珠流动分析系统研究（Ⅰ）

将荧光免疫分析技术、光纤传感技术、流动注射技术和免疫磁珠分离技术联用,建立了一种新型荧光光纤免疫磁珠流动分析系统,并采用荧光素异硫氰酸酯（FITC）对系统性能进行考察,结果表明该分析系统设计合理,性能可靠,在临床、环保等领域有广泛的应用前景。     

药物免疫分析及其进展

本文对用于检测药物的免疫技术作了概述，并总结了近年来一些分析体系的改进和发展，同时指出了现代药物免疫分析的一些新的发展趋势，其中包括多分析物免疫分析方法，新型均相免疫分析方法，以及色谱、流动注射技术等在药物免疫分析中的应用。免疫分析技术可使今后药物定量检测更为灵敏，快速。     

化学发光免疫分析新进展

本文综述了2007年至今国内外化学发光免疫分析(Chemiluminescence immunoassay,CLIA)的理论研究成果及应用进展,分别从化学发光反应体系、基因工程试剂、新型固相材料、化学发光免疫分析联用检测技术以及多组分化学发光免疫检测技术等方面进行了阐述,并对化学发光免疫分析技术的发展趋势进行了展望.引用文献113篇.     

新型荧光光纤免疫磁珠流动分析系统研究（I）

将荧光免疫分析技术、光纤传感技术、流动注射技术和免疫磁珠分离技术联用,建立了一种新型荧光光纤免疫磁珠流动分析系统,并采用荧光素异硫氰酸酯（ＦＩＴＣ）对系统性能进行考察,结果表明该分析系统设计合理,性能可靠,在临床、环保等领域有广泛的应用前景。     

化学发光免疫分析技术及其在农药残留检测中的应用进展

化学发光免疫分析技术发展迅速,具有特异性强、灵敏度高、线性范围宽、操作简单、成本底、检测时间短、易于实现自动化等优点,在农残检测领域得到广泛应用。以化学发光免疫分析为基础,介绍了吖啶酯类、鲁米诺类、咪唑类、苯酚类及芳基草酸酯类5种化学发光剂,及化学发光免疫分析法、荧光化学发光免疫分析法、化学发光酶联免疫分析法和电化学发光免疫分析法4种常见的分析方法,重点综述了化学发光免疫分析技术在农药残留检测中的应用,并对该领域的研究前景进行了展望,旨在为农药残留检测相关研究提供参考。     

电化学免疫分析法研究进展

电化学免疫分析法是将免疫分析与电化学分析技术相结合的一种免疫分析新方法，近十多年来，电化免疫分析的研究有了迅速的发展。本文对电化学的免疫分析法的标记物、免疫方法、电化学检测技术进行了概括总结，并展望了电化学免疫分析的发展前景。     

荧光偏振免疫分析技术的研究进展

对荧光偏振免疫分析技术的研究进展作了详细的评述。介绍了荧光偏振免疫分析技术的基本原理和研究热点,评述了它在临床检验、环境与食品监测和农药残留量分析等领域中的应用,对荧光偏振免疫分析方法的优缺点作了总结,引文献45篇。     

The kink-pair nucleation in edge dislocation motion

The motion of edge dislocations (EDs) and kinked EDs in body-centered cubic Fe are studied at atom level. We find the kink-pair nucleation mechanism directly from the atom configuration evolution of dislocation, the EDs move primarily by the kink-pair nucleation, and the pre-existing kink does not affect this motion mode. The result is in agreement with the energy calculation. Furthermore, the Peierls stress, dislocation velocity, and phonon-drag coefficient are discussed. There are obviously two motion regions with different stresses. In the low-stress region, the stress exercises a great influence on the dislocation motion, the dislocation velocity increase significantly with the increase of stress. In the high-stress region, however, phonon drag practice a dominant factor on the dislocation motion, the dislocation move with approximately constant speed close to the transverse speed of sound.     

Cationic polymerization of 1,3-pentadiene : IV. Initiation by aluminum chloride in an apolar solvent: effect of various electron donors

The polymerization of 1,3-pentadiene (PD) initiated by aluminum trichloride in nonpolar solvent was carried out in the presence of various electron donors (EDs) such as ethyl acetate, (EtOAc) tert-butyl acetate, dimethyl phthalate (DMP), N,N-dimethyl formamide, N,N-dimethyl acetamide and dimethyl sulfoxide. Addition of an ED whatever its nature to the polymerization medium, in a one-to-one molar ratio relatively to the Lewis acid, resulted in a decrease of the overall yield and an increased proportion of crosslinked polymer. The molecular weight distribution of the soluble fraction was narrower than that of polymerizations carried out without ED. The microstructure of the soluble polymers can be tuned using different EDs, showing that they are interacting with the active species. For instance EtOAc increased polymer isomerization while DMP increased polymer cyclization. Thus, the nature of the chemical functions borne by the ED does not seem to be the only parameter explaining its influence on the polyPD microstructure. If crosslinking efficiency seems to be roughly correlated to the donicity scale of the EDs, termination reaction is not. It was shown that the complexation between the Lewis acid and the EDs containing a carbonyl group involved the carbonyl oxygen atom. The decrease of polymerization yield when using the EDs was assigned to this complexation between the ED and AlCl₃.     

Validation and application of reporter gene assays for the determination of estrogenic and androgenic endocrine disruptor activity in sport supplements

Previously developed estrogen and androgen mammalian reporter gene assays (RGAs) were assessed for their potential use as a quantitative screening method in the detection of estrogenic and androgenic endocrine disruptors (EDs) in sport supplements. The validation of both RGAs coupled with dispersive solid phase extraction (dSPE) was performed in accordance with European Commission Decision EC/2002/6579 for biological screening methods. Decision limits (CCα) and detection capabilities (CCβ) were established for both the estrogen and androgen RGAs. All samples were compliant with CCα and CCβ in both bioassays. Recovery rates were 96 % for 17β-estradiol and 115 % for dihydrotestosterone as obtained in their corresponding RGA. Both estrogens and androgens were stable in samples for more than 3 weeks, when stored at -20 °C. Specificity, good repeatability (coefficients of variation (CV), 12-25 %), reproducibility and robustness of both bioassays were also observed. Four different ED modes of action were determined for estrogens and androgens in 53 sport supplements, using the validated RGAs. This study revealed that 89 % of the investigated sport supplements contained estrogenic EDs and 51 % contained androgenic compounds. In conclusion, both bioassays are suitable for sport supplement screening of estrogenic and androgenic EDs.     

免疫分析法进展

本文首先从试剂分析的观点对免及分析的原理进行了论.述,对抗体作为分析试剂进行了评价并且总结了标记免投分析的三种方式和四个环节;其次,对免疫分析的现状作了综述,并评述了当代免反分析的五个热点:基因工程抗体,生物素一亲合素多重标记体系,时间分辨荧光免疫分析,多组分免疫分析和自动化免疫分析;最后,对免疫分析的发展趋势作了讨论。     

微流控芯片免疫分析方法研究进展

综述了微流控芯片免疫分析方法研究新进展。对有关芯片进行了初步分类，并评述了各类芯片的性能与优缺点。尤为关注免疫分析微流控芯片在临床诊断、环境分析等领域的应用研究。引用文献33篇。     

多指标免疫分析的研究进展

免疫分析是临床上检测生理相关蛋白质指标的主要方法. 与单一指标的免疫分析相比, 临床体外诊断对多指标的生理相关蛋白质免疫分析有着更为广泛的需求. 通过在固相载体上完成免疫反应的非均相免疫分析具有灵敏度高的优点, 是当前多指标免疫分析的主流方法. 本文按照固相载体的不同, 对近年来的多指标免疫分析系统分别从技术原理、 实现方法、 各自特点等方面的研究进展进行综合阐述. 最后对比总结了不同系统的优势与不足, 并展望了微流控多指标免疫分析在即时检测领域的发展前景.     

电感耦合等离子体质谱用于多组分免疫分析研究进展

免疫分析在临床医学检测领域具有重要的地位.本课题组提出了基于电感耦合等离子体质谱(ICP-MS)的免疫分析方法,利用元素标记技术结合ICP-MS检测实现多组分免疫分析.随后,研究人员在该领域做了大量研究工作,证明该方法可用于从小分子、蛋白质、核酸到细胞等一系列生物样品的检测.本文综述了基于ICP-MS免疫分析方法的特点,对其发展方向进行了展望,希望为该领域的研究工作提供参考.     

Electron‐pair‐donor reaction order in the cationic polymerization of isobutylene coinitiated by AlCl3

The cationic polymerizations of isobutylene (IB) initiated by an H₂O/AlCl₃ system were carried out in a mixture of n‐hexane/methylene dichloride of 60/40 v/v at ?50 °C in the presence of various external electron pair donors (EDs), including methyl benzoate (MB), ethyl benzoate (EB), and methyl acrylate (MA). The effects of the concentrations of EDs ([ED]) and polymerization time on monomer conversion and kinetics of IB polymerization were also investigated. The complexes between AlCl₃ and the esters were soluble in the polymerization system at ?50 °C. The polymers with high molecular weights and relatively narrow molecular weight distributions were obtained in the presence of the EDs. The rate of polymerization decreased with increasing [ED]. The kinetic orders of the EDs were remarkably dependent on their chemical structure, steric hindrance from moieties, and concentration in the polymerization system. The reaction order of MB was determined to be around ?1.3 when [MB] ≤ 0.40 mmol/L, whereas it was ?12.9 when [MB] ≥ 0.40 mmol/L. The reaction order of EB was determined to be ?1.36 when [EB] ≤ 0.41 mmol/L, whereas it was ?3.36 when [EB] ≥ 0.41 mmol/L. The reaction order of MA was determined to be ?1.85 when [MA] ≤ 0.48 mmol/L, whereas it was ?16.7 when [MA] ≥ 0.48 mmol/L. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 3053–3061, 2007     

Facile method for surface etching of silica aerogel monoliths

Degradation of three different endocrine disruptors (EDs) was thoroughly studied on prepared durable thin layers of titanium dioxide with an anatase crystalline structure. Specially constructed laboratory reactors bringing information on all individual processes (photolysis, photocatalysis, sorption) involved in decomposition of the studied EDs (17α-ethynylestradiol, bisphenol A and 4-nonylphenol) were applied. It was found that photolytic removal of EDs is the fastest degradation process; nevertheless, this method may be less effective regarding all indicators including toxicity. It was verified that individual degradation processes (photolysis and photocatalysis) showed a significantly different influence on toxicity of resulting solutions. During the photolytic process, EDs degradation caused increasing toxicity contrary to the photocatalytic process. Obtained results were corroborated by a mathematical model, which showed that a limitation step for photocatalysis is a sorption and for photolysis a toxicity of resulting products.

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