High-Performance Thin-Layer Chromatographic Method for the Simultaneous Quantification of Piperine,Piperlongumine, and 6-Shogaol in Trikatu Churna — An Ayurvedic Formulation

JPC – Journal of Planar Chromatography – Modern TLC - Trikatu Churna is an important formulation in Ayurveda — the Traditional System of Indian Medicine. It consists of fine...     

Effective Separation and Simultaneous Quantification of Permethrin Isomers in Household Products by Validated TLC—Densitometric Method

JPC – Journal of Planar Chromatography – Modern TLC - Permethrin is widely used in household pests and insect-controlling products. A sensitive and robust TLC-densitometric method for...     

Determination of Fluoroquinolone Antibiotics in Industrial Wastewater by High-Pressure Liquid Chromatography and Thin-Layer Chromatography—Densitometric Methods

JPC – Journal of Planar Chromatography – Modern TLC - Two methods were described for the simultaneous determination of ciprofloxacin HCl (CIP) and moxifloxacin HCl (MOX) in their binary...     

Concurrent Analysis of the Biologically Active Markers β-Amyrin and β-Sitosterol by Applying a Validated High-Performance Thin-Layer Chromatography Method in the Aerial Parts of Tinospora cordifolia and Calotropis gigantia

JPC – Journal of Planar Chromatography – Modern TLC - In this study, we have developed a validated high-performance thin-layer chromatography (HPTLC) method for the concurrent...     

Thin-Layer Chromatography—Image Analysis Method for the Simultaneous Quantification of Andrographolide and Related Diterpenoids in Andrographis paniculata

JPC – Journal of Planar Chromatography – Modern TLC -     

Development and Validation of a High-Performance Thin-Layer Chromatographic—Densitometric Method for the Quantification of Apigenin in Ocimum basilicum L. Seed (Takhmaria)

JPC – Journal of Planar Chromatography – Modern TLC - A simple, selective, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been developed for the...     

Chemical Fingerprinting and Quantification of Biomarker Wedelolactone in Different Sources of “Bhringaraja” by High-Performance Thin-Layer Chromatography and Method Validation

JPC – Journal of Planar Chromatography – Modern TLC - Two different botanical sources, Eclipta alba and Wedelia calen-dulacea are used as “Bhringaraja” in the Ayurvedic...     

Quantification of α-Asarone in Acorus calamus by Validated HPTLC Densitometric Method

JPC – Journal of Planar Chromatography – Modern TLC -     

Development of a Validated High-Performance Thin-Layer Chromatography Method for the Simultaneous Estimation of Caffeic Acid,Ferulic Acid, β-Sitosterol,and Lupeol in Convolvulus pluricaulis Choisy and Its Adulterants/Substitutes

JPC – Journal of Planar Chromatography – Modern TLC - The present paper reports a validated high-performance thin-layer chromatography (HPTLC)—densitometric method for the...     

Application of Validated TLC—Densitometric Method for Simultaneous Identification and Determination of Losartan Potassium,Telmisartan, and Valsartan in Tablets

JPC – Journal of Planar Chromatography – Modern TLC - The antihypertensive effect of sartans is a result of inhibition of the binding of angiotensin (AT) II to the AT1 receptor in...     

Development and Validation of a High-Performance Thin-Layer Chromatographic—Densitometric Method for the Quantification of Guggulsterones E and Z in a Polyherbal Formulation Containing Commiphora Mukul

JPC – Journal of Planar Chromatography – Modern TLC - The present article focuses on the quantitative analysis of a polyherbal formulation containing arjuna, guggul, and pushkarmoola in...     

Standardization of Biomarkers Gallic Acid and Berberine in Polyherbal Formulation Entoban Capsules by High-Performance Thin-Layer Chromatography—Densitometry

JPC – Journal of Planar Chromatography – Modern TLC - The technological improvement in the structural elucidation of natural compounds has made it probable to generate appropriate...     

High-Performance Thin-Layer Chromatography and Indirect TLC—HRMS-Based Determination of 20-Hydroxyecdysone in Sesuvium portulacastrum

JPC – Journal of Planar Chromatography – Modern TLC - 20-Hydroxyecdysone (20E), an imperative phytoecdysteroid, regulates several biochemical and physiological processes during the...     

Development and Validation of a High-Performance Thin-Layer Chromatography—Densitometry Method for the Quantitative Estimation of Morphine in the Classical Ayurvedic Formulation Kamini Vidrawan Ras

JPC – Journal of Planar Chromatography – Modern TLC - Kamini Vidrawan Ras is a classical Ayurvedic medicine, referred to in Bhaisjya Ratnavali, a book recognized by the Drugs and...     

Rapid Validated Thin-Layer Chromatography—Densitometry for the Simultaneous Determination of Three Co-formulated Drugs Used for Common Cold Treatment

JPC – Journal of Planar Chromatography – Modern TLC - A rapid validated thin-layer chromatography (TLC)—densitometric method has been developed for the simultaneous determination...     

Validated Simultaneous High-Performance Thin-Layer Chromatographic Analysis of Ursolic Acid, β-Sitosterol,Lupeol and Quercetin in the Methanolic Fraction of Ichnocarpus frutescens

JPC – Journal of Planar Chromatography – Modern TLC - A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination and validation of...     

Quantitation of the Flavonols Quercetin and Kaempferol in the Leaves of Trigonella foenum-graecum by High-Performance Liquid Chromatography – Diode Array Detection

Trigonella foenum-graecum is known for the strong hypoglycemic effects of the seed extract and for its significant allelopathic effects. The leaf extract however has not been studied thoroughly up-to-date and no data are available regarding the quantitative content of the plant's leaves. The purpose of this study was to quantify the flavonoid content and to assess the flavonoid seasonal variation in the leaves of the plant during the three developmental stages, vegetative, flowering, and fruiting. An HPLC method has been developed and validated employing a reversed phase C18 column and Diode Array Detection. Sample pre-treatment was performed by methanol:water (80:20 v:v) extraction and glucoside hydrolysis with HCl. The method allowed the reliable determination of the aforementioned substances in the Trigonella's leaves revealing the highest amount of flavonoids during the flowering stage.     

Determination of Ranolazine in Tablet Formulations by High-Performance Thin-Layer Chromatography—Mass Spectrometry Using Reflectance Scanning Densitometry

JPC – Journal of Planar Chromatography – Modern TLC - A new quantitative densitometric high-performance thin-layer chromatographic (HPTLC) method was developed and validated for the...     

GC–MS Analysis of the Essential Oils of Piper nigrum L. and Piper longum L.

A simple, rapid and solvent-free method based on gas chromatography–mass spectrometry (GC–MS) following microwave distillation and headspace solid-phase microextraction (MD–HS-SPME) was developed for the analysis of the essential oils in two traditional Chinese medicines, Piper nigrum L. and Piper longum L. Thirty compounds were separated and identified from P. nigrum L. The main components were β-caryophyllene (23.49%), 3-carene (22.20%), d-limonene (18.68%), β-pinene (8.92%) and α-pinene (4.03 %). Forty-five compounds were separated from P. longum L. and identified. The main components were β-caryophyllene (33.44%), 3-carene (7.58%), eugenol (7.39%), d-limonene (6.70%), zingiberene (6.68%) and cubenol (3.64%). To demonstrate its advantages, MD–HS-SPME was compared to conventional HS-SPME. With conventional HS-SPME, only 28 and 33 compounds were detected in P. nigrum L. and P. longum L, respectively. Relative standard deviation (RSD) values of MD–HS-SPME for the essential oils in P. nigrum L. under optimal conditions were less than 10%. The results show that microwave distillation has a high extract efficiency and good precision and can be used to compare similarities and differences of essential oils.     

Application of Two-Dimensional High-Performance Thin-Layer Chromatography to Qualitative and Quantitative Toxicological—Chemical Analysis

JPC – Journal of Planar Chromatography – Modern TLC - The present paper describes various application possibilities of high-performance thin-layer chromatography (HPTLC) for qualitative...