Wound-healing activity of an oligomer of alkannin/shikonin,isolated from root bark of Onosma echioides

Root bark of Onosma echioides belonging to the family Boraginaceae is reported to be rich in naphthaquinones such as alkannins and shikonins. In this study, a dimer of alkannin/shikonin was isolated from the petroleum ether (60–80 C) extract of the bark, and the structure of the same was elucidated through spectral studies (UV, IR, NMR, MS and DEPT). The petroleum ether extract was found to contain 62.4% (w/w) of the dimer of alkannin/shikonin, and the compound is found to promote wound-healing process, when studied in the excision and incision wound models in albino rats.     

高效液相色谱法测定银杏叶提取物中槲皮素的含量

An HPLC method has been developed for the determination of quercetin,one of flavones,in extract ofGinkgo b1loba L,leaves.Quercetin was separated completely on a Zorbax-ODS column, 250mm× 4. 6mm i.d.,using a mixture of methanol, water and phosphoric acid(55:44. 6:0.4)as mobile phase; with UV detec-tion at 254nm. The results have shown that the recoveries were between 93.3～103.3%andCVwas l.4l%。     

男性精液生殖细胞精蛋白的高效液相色谱分离研究

HPLC has been successfully used to the separation of protamines in sperm cells of fertile and infertilemen. The separation was carried out with TSK G3000 SW column,2mol/L ureaand UV detector at 216nm. From these chromatographic tests here it is shown that there haven’t been enough protamines in sperm cellsof infertile men by compared with fertile men. And this method can be used for assay of protamines in spermcells and is proved to be simple,accurate and sensitive.     

高效液相色谱法测定硝苯柳胺含量

硝苯柳胺是合成筛选出来的一种新杀螺剂。经实验探索,以３,５－二硝基苯甲酸作内标,采用并建立了高效液相色谱法测定合成物中硝苯柳胺的含量。方法简便、准确,灵敏度高。     

高效液相色谱法测定22S，23S，24R表高油菜素内酯

The sample was dissolved in ethyl acetate and reactcd with pbenylboronic acid at 80°for 3Omin.Then itwas detemined by HPLC using UV detector at 222nm. The linear relations between the amounts and thepeak areas in the range of 0. 02mg/mL to 0.12mg/mL were staisfactory(γ=0. 9999,n=6). Average recovery was 99.27%～101.5%.     

麦草伏甲酯对对映体的高效液相色谱分离

手性分离;麦草伏甲酯对对映体的高效液相色谱分离     

高效液相色谱法鉴别不同的分散染料

用高效液相色谱法测定了１００余种不同来源的分散染料。色谱柱为ＮｏｖａｐａｋＣ_（１８）柱（１５０ｍｍ×３．９ｍｍｉ．ｄ．）,流动相为甲醇－水（８５：１５,Ｖ／Ｖ）,检测波长为４３６ｎｍ和５４６ｎｍ。     

高效液相色谱法检测弹性蛋白中锁链素

用高效液相色谱技术对弹性蛋白水解液中锁链素进行了测定。单一锁链素,锁链素、酪氨酸、苯丙氨酸混合液及弹性蛋白水解液经Ｃ１８柱分离,甲醇－水（２８,Ｖ／Ｖ）洗脱,紫外检测器２８０ｎｍ检测。锁链素与酪氨酸峰面积比（ＣＡ）和峰高比（ＣＨ）分别为１．４％和３．４％,标准曲线浓度范围０．１６～０．６４ｍｇ／Ｌ时相关系数为０．９９５６。结果表明：上述色谱条件可定量测定弹性蛋白中锁链素含量,无需将锁链素与水解液中其它氨基酸加以分离,而仅需同其它２７５ｎｍ有吸收的组分区分。     

高效液相色谱法测定唾液中替硝唑浓度

用高效液相色谱法测定了唾液中替硝唑的浓度,以甲硝唑为内标,高氯酸沉淀蛋白后直接进样。方法简便、快速、回收率高、重现性好。     

高效液相色谱法测定联苯取代衍生物的三种异构体

A method for the separation and deternunation of three isomers of 4-nitro-methyldiphenyl by reversed-phase HPLC is described.The method for quantitative analysis without standard substance was studied with satisfactory results.     

高效液相色谱法测定萨拉沙星

采用高效液相色谱法测定了萨拉沙星。色谱柱为μ－BondapakTMC18柱（3．9mm×300mm）,流动相为V（乙腈）：V（甲醇）：V（2mmol／L磷酸,用三乙胺调pH3．5）＝30：5：65,用二极管阵列检测器检测,检测波长278nm,得到了满意的分离效果。     

C_（60），C_（70）液相色谱分离方法的研究

较系统地考察了不同柱系统及不同流动相组成下Ｃ＿（６０）,Ｃ＿（７０）的保留规律。在实验的基础上,提出了分离Ｃ＿（６０）,Ｃ＿（７０）的最佳柱系统及流动相组成。并在此分离条件下,对高分子量的富勒烯组分进行了分离。     

高效液相色谱法测定人血清中格列齐特

A rapid and simple HPLC method was developed for the quantitative analysis of gliclazide in humanserum. After being extracted with benzene, gliclazide was analyzed by reversedphase HPLC colurnn (YWG-C(18)H(37)) and UV-detector(229nm) with tolbutamide as internal standard. The mobile phase was a mixture ofmethyl alcohol and 0.2%glacial acetic acid solution(5: 4 ) at a rate of 1mL/min. Over the concentrationrange of 0.25～32μmol/L, the linear regression equation was : Y=-0. 008412+0.1817X(n=8,r=0. 9996).     

HPLC法测定鸡精中谷氨酸钠的含量

以邻苯二甲醛与谷氨酸钠中的氨基进行柱前在线衍生化反应,采用C18色谱柱分离、荧光检测器（激发340nm,发射450nm）进行测定,建立了柱前衍生反相高效液相色谱测定鸡精中谷氨酸钠含量的方法。该方法相对标准偏差为0．69％,加标回收率为99．1％～101％,在0．10~50．0mg／L范围内,谷氨酸钠的峰面积和浓度之间的相关系数为0．9999,保留时间和峰面积的相对标准偏差分别为1．22％和0．71％,鸡精中谷氨酸钠定量下限为0．2μg／g。     

高效液相色谱法测定依托咪酯异构体杂质

建立了依托咪酯异构体杂质的高效液相色谱（HPLC）方法,采用AD-H柱,流速0.8 mL/min,检测波长235 nm.经方法学验证,该方法对依托咪酯及其异构体专属性确认分离度大于2.0,对依托咪酯异构体杂质的最小检测限（LOD）为0.002％,线性范围为0.001 6.μg～0.052 6 μg,相关系数r=0.999 44,加样回收率分别为82.0％、90.5％和96.2％.结果表明该检测方法专属性好,灵敏度高,准确,用于依托咪酯异构体杂质测定结果可靠.     

高效液相色谱法测定烟叶中茄尼醇的含量

选用Ｚｏｒｂａｘ－ＳＩＬ（２５０ｍｍ×４．６ｍｍｉ．ｄ．）色谱柱,以正己烷－异丙醚－乙酸乙酯（６３１）混合溶剂作流动相,用示差折光检测器测定了烟叶提取物中茄尼醇的含量,结果表明所用方法具有良好的分离效果和回收率,在０．２～４μｇ之间有良好的线性关系,可用于烟叶或茄尼醇产品中茄尼醇含量的测定。     

二维高效液相色谱技术用于分析中药制剂中麻黄类生物碱

构建了一个离线的二维高效液相色谱系统,以解决成分复杂的中药样品的分离和痕量组分的定量问题。该系统在原有的单泵、单检测器高效液相色谱仪的基础上作了相应的改进而构成,样品先在第一维的Zor-bax SB-C18色谱柱中进行分离之后,利用一个六通切换阀将目标组分切换并收集,浓缩后再注入第二维的Ai-chrom Bond-AQ C18色谱柱中进行分离。利用上述方法对3种麻黄类生物碱进行了二维分离和分析,实验结果表明,样品的分离明显改善,并且这个系统可用于中药的直接进样分析。     

高效液相色谱法测定超滤后血清中的假尿苷

用高效液相色谱法（ＨＰＬＣ）直接测定了超滤后血清中的假尿苷（ＰＳＥ）,平均回收率为９７．７８％,在０．０９３～６μｍｏｌ范围内呈线性关系（γ＝０．９９５６）,急性白血病患者血中ＰＳＥ水平显著高于正常对照组（Ｐ＜０．０１）。该方法简单、方便、迅速,可用于恶性病的病情及疗效监测。     

高效液相色谱测定人血清中鬼臼乙叉甙

A rapid and simple HPLC method was developed for the quantitative analysis of etopo-side in human serum. After being extracted with ethyl acetate, etoposide was analyzed by re-versed-phase HPLC column(YWG-C_(18)H_(37)) and UV-detector(230nm).The mobile phase wasamixture of water-acetonitrile-glacial acetic acid(64: 35 :1),with a flow rate of 1mL/min. Over the concentration range of 0. 085～85μmol/L, the linear regression equation was:Y=292.17X+29.50(n=8,γ=0. 9902).     

高效液相色谱法测定果汁饮料中的维生素C含量

建立饮料中维生素C含量的高效液相色谱检测方法。采用ODS C18(4.6mm×250mm,5μm)反相色谱柱,以0.1%草酸溶液为流动相,光电二级管阵列检测器,检测波长为267nm。线性范围为20～160μg/mL,相关系数为0.9983,方法的回收率为96.4%～100.2%,相对标准偏差为0.61%。该方法简便、分析速度快,能够满足果汁饮料中维生素C的检测要求。