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Recently, five indole prenyltransferases from Aspergillus fumigatus have been proven biochemically to be responsible for prenylations of diverse substrates. In this study, we show peptidase activities of 7-DMATS, FgaPT1, CdpNPT, and FtmPT1, with preference for linear peptides containing a tryptophanyl moiety at the N terminus. Testing of 31 peptides revealed that these enzymes shared similar substrate specificity and accepted H-L-Trp-L-Ala-OH and H-L-Trp-Gly-OH as best substrates for aminopeptidase activity. By using H-L-Trp-Gly-OH as substrate, Km values at 350, 380, 300, and 420 microM and enzymatic rate constants kcat/Km at 0.51, 0.24, 0.53, and 0.14 mM(-1)s(-1) were determined for 7-DMATS, FgaPT1, CdpNPT, and FtmPT1, respectively. In contrast to prenyltransferase activities, the aminopeptidase activities were strongly or completely inhibited by EDTA. Mn2+ increased the aminopeptidase activities of FtmPT1 and CdpNPT up to 4- and 6-fold, respectively. To the best of our knowledge, this is the first report on the catalytic promiscuity of prenyltransferases. 相似文献
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GuoYingZUO HongPingHE BinGuiWANG XinHONG YiMingHU XiaoJiangHAO 《中国化学快报》2003,14(4):383-384
A new diterpenoid,15-0-accetylspiraminol(1),was isolated from the aerial parts of Spireae japonica L.f.var.ovalifolia.The structure was charaterized mainly based on spectral analysis. 相似文献
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New Diterpenoid Alkaloids from Spiraea japonica var. Ovalifolia 总被引:2,自引:0,他引:2
Guo Ying ZUO Hong Ping HE Xin HONG Wei Ming ZHU Yi Ming HU Xiao Sheng YANG Xiao Jiang HAO* Laboratory of Phytochemistry Kunming Institute of Botany Chinese Academy of Sciences Kunming Kunming Hospital PLA Kunming 《中国化学快报》2001,(2)
In the investigation of the chemical constituents of Spireae japonica L. f. var. ovalifolia (Rosaceae), a shrub originated in Songming, Yunnan, five new diterpenoid alkaloids, 19-O-deethylspiramine N (1), deacetylspiramine S (2), spiramidine A (3), spiramidine B (4) and deacetylspiramine F (5), were isolated from aerial parts of the plant and structurally elucidated. Compound 1, a white amorphous powder with [(]+17.99 (c, 0.26, MeOH ), was determined to have the molecular formula of C20H… 相似文献
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Two new diterpenoids from Rabdosia japonica var.glaucocalyx 总被引:1,自引:0,他引:1
Two new diterpenoids, named glaucocalyxin F and glaucocalyxin X, respectively, were isolated from the whole plant of Rabdosia japonica (Burm. f.) Hara var. glaucocalyx (Maxim.) Hara. Their structures was established on the basis of spectral analysis. 相似文献
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Hai‐Yang Liu Suo Gao Ying‐Tong Di Chang‐Xiang Chen Yang Lü Li Zhang Qi‐Tai Zheng Xiao‐Jiang Hao 《Helvetica chimica acta》2007,90(7):1386-1390
A novel ent‐atisane lactone, spiramilactone E ( 1 ), was isolated from Spiraea japonica var. acuta Yu . Its structure was elucidated by extensive spectroscopic analyses, and unequivocally confirmed by single‐crystal X‐ray diffraction (Fig. 2). Compound 1 contains a γ‐lactone moiety between the 6‐OH function and C(20), and β‐configuration for the 7‐OH group, in contrast to known related diterpenes previously isolated from the S. japonica complex. 相似文献
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An extract of Keiskea japonica MIQ. showed an inhibitory effect on hyaluronidase activity. From the extract, four new phenylpropanoids, two new maltol glycosides, two new monoterpene glycosides, and two new phenolic compounds were isolated together with 19 known compounds. Among these constituents, two phenylpropanoids and a flavone glucuronide were revealed as hyaluronidase inhibitors. 相似文献
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Six prenylated phenols, sabphenols A and B ( 1 and 2 , resp.), and sabphenosides A–D ( 3 – 6 , resp.), along with eight known constituents, were isolated from the rhizomes of Sabia japonica Max . Their structures were established on the basis of 1D‐ and 2D‐NMR spectral analysis. 相似文献
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Xin‐Zhou Yang Li‐Gen Lin Chun‐Ping Tang Yong‐Qiang Liu Yang Ye 《Helvetica chimica acta》2007,90(2):318-325
Five new nonalkaloid constituents, a neolignan, japonin A ( 1 ), a macrocyclic lactone, japonin B ( 2 ), a (phenylethyl)benzoquinone, japonin C ( 3 ), a phenanthraquinone, japonin D ( 4 ), as well as a dihydrostilbene, stilbostemin M ( 5 ), were isolated from the roots of Stemona japonica, together with eight known compounds. Their structures were established by spectroscopic analyses. 相似文献
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Summary 1. FromInula japonica a new sesquiterpene lactone — deacetylinulicin — has been isolated.2. Spatial formulas have been put forward for inulicin and deacetylinulicin (Ia and Ib, respectively).All-Union Scientific-Research Institute of Medicinal Plants. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 730–733, November–December, 1974. 相似文献
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Phenolic glycosides from Pyrola japonica 总被引:2,自引:0,他引:2
Kim JS Shim SH Xu YN Kang SS Son KH Chang HW Kim HP Bae K 《Chemical & pharmaceutical bulletin》2004,52(6):714-717
Five new phenolic glycosides, 2-beta-D-glucopyranosyloxy-5-hydroxyphenylacetic acid methyl ester (4), 4-hydroxy-2-[3-hydroxy-3-methylbutyl]-5-methylphenyl beta-D-glucopyranoside (5), 4-hydroxy-2-[(E)-4-hydroxy-3-methyl-2-butenyl]-5-methylphenyl beta-D-glucopyranoside (7), 4-hydroxy-2-[(2E,6Z)-8-beta-D-glucopyranosyloxy-3,7-dimethylocta-2,6-dien-1-yl]-5-methylphenyl beta-D-glucopyranoside (8), and 2,7-dimethyl-1,4-dihydronaphthalene-5,8-diol 5-O-beta-D-xylopyranosyl(1-->6)-beta-D-glucopyranoside (10), were isolated from the whole plants of Pyrola japonica (Pyrolaceae), together with androsin, (-)-syringaresinol glucoside, homoarbutin, pirolatin, hyperin, monotropein and chimaphilin. 相似文献