共查询到20条相似文献,搜索用时 31 毫秒
1.
Afroditi Papasavva Antonio Shegani Christos Kiritsis Ioanna Roupa Myrto Ischyropoulou Konstantina Makrypidi Irineos Pilatis George Loudos Maria Pelecanou Minas Papadopoulos Ioannis Pirmettis 《Molecules (Basel, Switzerland)》2021,26(16)
Sentinel lymph node detection (SLND) is rapidly entering common practice in the management of patients with tumors. The introduction of mannose molecules to 99mTc-labeled dextrans, so far, showed that the sentinel node could trap these agents due to their recognition by the mannose receptors of lymph node macrophages. The current study aimed to synthesize, characterize, and biologically evaluate a series of mannosylated dextran derivatives labeled with 99mTc for potential use in SLND. The compounds were designed to have a dextran with a molecular weight of 10–500 kDa as a backbone, S-derivatized cysteines, efficient SNO chelators, and mannose moieties for binding to mannose receptors. They were successfully synthesized, thoroughly characterized using NMR techniques, and labeled with the fac-[99mTc(CO)3]+ synthon. Labeling with high yields and radiochemical purities was achieved with all derivatives. In vivo biodistribution and imaging studies demonstrated high uptake in the first lymph node and low uptakes in the following node and confirmed the ability to visualize the SLN. Among the compounds studied, 99mTc-D75CM demonstrated the most attractive biological features, and in combination with the high radiochemical yield and stability of the compound, its further evaluation as a new radiopharmaceutical for sentinel lymph node detection was justified. 相似文献
2.
Rubel Chakravarty Meera Venkatesh Ashutosh Dash 《Journal of Radioanalytical and Nuclear Chemistry》2011,290(1):45-51
A novel electrochemical process to avail clinical grade 99mTc from (n,γ)99Mo has been demonstrated. The electrochemical parameters were optimized to maximize the 99mTc yield with minimal 99Mo contamination. 99Mo/99mTc generators containing up to 29.6 GBq (800 mCi) 99Mo were developed and their performance were extensively evaluated for 10 days without changing the operating conditions.
Very high radioactive concentration of 99mTcO4
− of acceptable quality, commensurate with hospital radiopharmacy requirements could be availed from the system with >90% yield.
The compatibility of the product for the formulation of 99mTc labeled radiopharmaceuticals such as 99mTc-DMSA and 99mTc-EC was found to be satisfactory in terms of high labeling yields. The proposed route represents an important step for enhancing
the scope of accessing clinical grade 99mTc from low specific activity (n, γ)99Mo. 相似文献
3.
Evren Gundogdu Derya Ilem-Ozdemir Makbule Asikoglu 《Journal of Radioanalytical and Nuclear Chemistry》2014,299(3):1255-1260
Bisphosphonates can be labeled with Technetium-99m (99mTc) and are used for bone imaging because of their good localization in the skeleton and rapid clearance from soft tissues. Over the last decades bone scintigraphy has been used extensively in the evaluation of oncological patients to provide information about the sites of bone lesions, their prognosis and the effectiveness of therapy by showing the sequential changes in tracer uptake. Since the lesion visualization and lesion/bone ratio are important utilities for a bone scanning radiopharmaceutic; in this study incorporation of 99mTc labeled alendronate sodium (99mTc–ALD) was evaluated in U2OS (human bone osteosarcoma) and NCI-H209 (human bone carcinoma) cell lines. ALD was directly labeled by 99mTc, radiochemical purity and stability of the complex were analyzed by radioactive thin layer chromatography and radioactive high performance liquid chromatography studies. For cell incorporation study, NCI-H209 and U2OS cell lines were used with standard cell culture methods. The six well plates were used for all experiments and the integrity of each cell monolayer was checked by measuring its transepithelial electrical resistance (TEER) with an epithelial voltammeter. Results confirmed that ALD was successfully radiolabeled with 99mTc. 99mTc–ALD incorporated with NCI-H209 and U2OS cells. The uptake percentages of 99mTc–ALD in NCI-H209 and U2OS cell lines were found significantly different. Since 99mTc–ALD highly uptake in cancer cell line, the results demonstrated that radiolabeled ALD may be a promising agent for bone cancer diagnosis. 相似文献
4.
Saeed Shanehsazzadeh Mohammad Ali Oghabian Fariba Johari Daha Massoud Amanlou Barry J. Allen 《Journal of Radioanalytical and Nuclear Chemistry》2013,295(2):1517-1523
Recently ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles (NPs) have been widely used for medical applications. One of their important applications is using these particles as MRI contrast agent. While various research works have been done about MRI application of USPIOs, there is limited research about their uptakes in various organs. The aim of this study was to evaluate the biodistribution of dextran coated iron oxide NPs labelled with 99mTc in various organs via intravenous injection in Balb/c mice. The magnetite NPs were dispersed in phosphate buffered saline and SnCl2 which was used as a reduction reagent. Subsequently, the radioisotope 99mTc was mixed directly into the reaction solution. The labeling efficiency of USPIOs labeled with 99mTc, was above 99 %. Sixty mice were sacrificed at 12 different time points (From 1 min to 48 h post injections; five mice at each time). The percentage of injected dose per gram of each organ was measured by direct counting for 19 harvested organs of the mice. The biodistribution of 99mTc-USPIO in Balb/c mice showed dramatic uptake in reticuloendothelial system. Accordingly, about 75 percent of injected dose was found in spleen and liver at 15 min post injection. More than 24 % of the NPs remain in liver after 48 h post-injection and their clearance is so fast in other organs. The results suggest that USPIOs as characterized in our study can be potentially used as contrast agent in MR Imaging, distributing reticuloendothelial system specially spleen and liver. 相似文献
5.
Ismail Taha Ibrahim 《Journal of Radioanalytical and Nuclear Chemistry》2009,281(3):669-674
Metronidazole (MTNZ) is an antiprotozoa drug, could be labeled with the 99mTc. MTZL could be used as an ideal vehicle to deliver radioactive decay energy of 99mTc to the sites of tumor, thus facilitate tumor imaging. The process of labeling was done using tin chloride as reducing agent.
The optimum conditions required to label 25 μg MTZL were 100 μg stannous chloride, 30 min reaction time, room temperature
at pH 7–9 using 0.5 M phosphate buffer. The radiochemical purity of the labeled compound, at the above conditions, was determined
using paper chromatography. The yield was about 93%. About 2.5 × l06 of Ehrlich Ascites Carcinoma (EAC) was injected intrapritoneally (i.p) to produce ascites and intramuscularly (i.m) in the
right thigh to produce solid tumor in female mice. Biodistribution studies were carried out by injecting solution of 99mTc-MTZL in normal and tumor bearing mice. The uptake in ascites was over 5% of the injected dose per gram tissue body weight,
at 4 h post injection and above 4% in solid tumor. These data revealed localization of the tracer in the tumor tissues with
high percentage sufficient to use 99 mTc MTZL as promising tool for diagnosis of tumor. 相似文献
6.
D. Arginelli J. Heikkonen A. Miranti C. Peroni S. Ridone L. Vigna 《Journal of Radioanalytical and Nuclear Chemistry》2009,279(1):281-285
Large columns containing aluminum oxide (Al2O3) or gel (e.g. zirconium molybdate) are needed to prepare 98Mo(n,γ)99Mo→99mTc column chromatographic generators that results in large elution volumes containing relatively high 99Mo impurity and low concentrations of 99mTc. The decrease in radioactive concentration or specific volume concentration of 99mTc places a limitation on some pharmaceutical kits (DTPA, MIBI, ECD, etc.) or clinical procedures. We report on the post elution
concentration of 99mTc using in house prepared lead cation-exchange and alumina columns. Using these columns high bolus volumes (10–60 mL 0.02M
sodium sulfate) of 99mTc can conveniently be concentrated in 1 mL of physiological saline. This approach also works very effectively to prepare
high specific volume solutions of 99mTc-pertechnetate from a fission based 99Mo/99mTc generator in the second week of its normal working life. 相似文献
7.
Diethylenetriamine pentaacetic acid (DTPA) was labeled with 99mTc in three different ways, resulting in ‘classic’ 99mTc-DTPA, 99mTc(CO)3-DTPA and 99mTc(CO)2(NO)-DTPA. The biodistribution of the formed DTPA-complexes was studied in mice with a special emphasis on the behavior of the novel tricarbonyl and dicarbonyl-nitrosyl complexes, which was clearly differing from that of ‘classic’ 99mTc-DTPA. The conversion of a Tc-tricarbonyl complex to a Tc-dicarbonyl-nitrosyl complex using NO+ reagents offers a synthetic tool for preparing a novel class of 99mTc labeled compounds. 相似文献
8.
Mamdouh S. Masoud Amina A. Soayed Ahmed S. Elkholany 《Journal of Radioanalytical and Nuclear Chemistry》2012,292(3):1123-1130
The reaction of 99mTc of different oxidation states (+7, +4) with 2-thiouracil and 5-nitrobarbituric acid have been studied at different temperatures,
pH and concentrations. The reaction mixtures have been analyzed at different times using thin layer chromatography (TLC) and
a radio detector to show the peaks at the plates. 99mTc is obtained from the Mo generators with oxidation state (+7). The use of SnCl2 as a reducing agent gave 99mTc with oxidation state (+4). It is very difficult to separate the complexes formed from the reactions in very small concentration.
The percentage of 99mTc and its oxidation state involved in the complexes can be determined. The labeling efficiencies (percentage of complex)
in the reaction of 99mTc+7 with 5-nitro-barbituric-acid increases mostly at pH 10. Both oxidation states of 99mTc(+7, +4) can be detected at pH’s 4 and 10, but at pH 4, the reduced form 99mTCO2, is more pronounced. At pH 7 no complexes were detected and most of 99mTc remains as 99mTCO4
−
. By increasing the ligand concentration, the labeling efficiencies of the complex increases. For the reaction of 99mTc of oxidation states (+4, +7) with 2-thiouracil at different temperatures and analytical times it is concluded that several
complexes with different Rf values were observed in equilibrium and most of these complexes were unstable. 相似文献
9.
The chemical condition of99mTc eluate obtained from a99Mo-99mTc generator is a function of the source, time elapsed after elution and age of the eluate. The radiochemical purity and stability of99mTc labeled MAb-170 (Tru-Scint®ADTM, photoactivated monoclonal antibody kit) preparations was evaluated comparing pertechnetate source of known age and elution history. The effect of H2O2, a radiolytic impurity in99mTc eluates, on the active kit components stannous ion and photoactivated MAb and radiolabeling, yield has been investigated. The lyophilized Tru-Scint® ADTM kit has been labeled with 20 to 80 mCi in 0.5 to 4.0 ml of Sodium Pertechnetate99mTc Injection, USP. The eluates were obtained from three brands of generators and used up to six hours after elution. The kits were reconstituted either with Sodium Pertechnetate99mTc Injection, USP or Sodium Chloride Injection, USP, 0.9% containing known amounts of H2O2. The reconstituted kits were analyzed for radiolabeling yield and radiochemical impurities, stannous ion and protein sulfhydryl group. The results indicated that the radiolabeling yield is a function of both the chemical condition of99mTc eluate, generator brand and the radiolabeling parameters like reconstitution volume and activity. The observed radiolabeling yield differences did not depend on the amount of chemical technetium in the eluate. The major radiochemical impurities at 15-minute post labeling have been identified as the99mTc-buffer complex and column adsorbed reduced99mTc (99mTc-Ad) species and not the unreduced99mTcO
4
–
. 相似文献
10.
J. H. Chiu T. C. Chu P. S. Weng 《Journal of Radioanalytical and Nuclear Chemistry》1991,150(2):493-507
A radiochemical method has been developed for the determination of99Tc in low-level radioactive, waste from nuclear facilities, using99mTc as an internal tracer. Radioactive contaminants were removed by carrier hydroxide precipitation and chelating extraction with NaDDC/CHCl3 system at pH 4. The final technetium was chelated with NaDDC in 3N HCl solution and extracted selectively into chloroform. The average of radiochemical recovery for various types of LLW sample is about 90%. The decontamination factors for most radioactive nuclides are higher than 105. The detection limit for99Tc in a sample of about 10 g is 0.17 pCi/g (6.5 Bq·kg–1) for a 100-minute count. 相似文献
11.
M. A. Motaleb M. T. El-Kolaly H. M. Rashed A. Abd El-Bary 《Journal of Radioanalytical and Nuclear Chemistry》2012,292(2):629-635
Paroxetine (a selective serotonin reuptake inhibitor) was successfully labeled with 125I via direct electrophilic substitution reaction at ambient temperature. The reaction parameters studied were paroxetine amount,
CAT amount, pH of the reaction mixture, reaction temperature, reaction time and in vitro stability of 125I-paroxetine. 125I-paroxetine was obtained with a maximum labeling yield of 94 ± 0.23% and in vitro stability up to 24 h. Biodistribution studies
showed that maximum in vivo uptake of 125I-paroxetine in lungs was 27.89 ± 1.03% injected activity/g tissue at 15 min post-injection and retention in lungs remained
high up to 1 h, whereas the clearance from mice appeared to proceed mainly via the hepatobiliary pathway. 125I-paroxetine is not a blood product and so it is more safe than the currently available 99mTc-macroaggregated albumin (99mTc-MAA), and its lung uptake is higher than that of the recently discovered 99mTc(CO)5I and 99mTc-DHPM. As a conclusion, radioiodinated paroxetine could be used as a novel radiopharmaceutical for lung perfusion scan safer
than the currently available 99mTc-MAA and more potential than the recently discovered 99mTc(CO)5I and 99mTc-DHPM. 相似文献
12.
Ai-Yih Wang Chun-Liang Kuo Jiunn-Liang Lin Chao-Ming Fu Yuh-Feng Wang 《Journal of Radioanalytical and Nuclear Chemistry》2010,284(2):405-413
This study examined the applications of novel non-polymer magnetic ferrite nanoparticles (Fe3O4 NPs) labeled with 99mTc-pertechnetate (99mTcO4
−). The radiochemistry, chemistry, and biodistribution of Fe3O4 NPs labeled with 9mTcO4
− were analyzed. This paper employed instant thin layer chromatography and magnetic adsorption to evaluate the labeling efficiency
and stability of 99mTc-Fe3O4 at various reaction conditions. A scanning electron microscope, X-ray diffractometer, Fourier transform infrared spectrometer,
laser particle size analyzer, and superconducting quantum interference device magnetometer were used to analyze the physical
and chemical properties of the Fe3O4 and 99Tc-Fe3O4 nanoparticles. The biodistribution and excretion of 99mTc-Fe3O4 were also investigated. Radiochemical analyses showed that the labeling efficiency was over 92% after 1 min in the presence
of a reducing agent. Hydroxyl and amine groups covered the surface of the Fe3O4 particles. Therefore, 99Tc (VII) reduced to lower oxidation states and might bind to Fe3O4 NPs. The sizes of the 99Tc-Fe3O4 NPs were about 600 nm without ultrasound vibrations, and the particle sizes were reduced to 250 nm under ultrasound vibration
conditions. Nonetheless, Fe3O4 NPs and 99Tc-Fe3O4 NPs exhibited superparamagnetic properties, and the saturation magnetization values were about 55 and 47 emu/g, respectively.
The biodistribution showed that a portion of the 99mTc-Fe3O4 nanoparticles might embolize in a pulmonary capillary initially; the embolism radioactivity was cleared from the lungs and
was then taken up by the liver. 99mTc-Fe3O4 metabolized very slowly only 1–2% of the injected dose (ID) was excreted in urine and about 2.37% ID/g was retained in the
liver 4 h after injection. Radiopharmaceutically, 99mTc-Fe3O4 NPs displayed long-term retention, and only 99mTc-Fe3O4 NPs that dissociated to free pertechnetate could be excreted in urine. This research evaluated the feasibility of non-polymer
magnetic ferrite NPs labeled with technetium as potential radiopharmaceuticals in nuclear medicine. 相似文献
13.
Three methods have been used for the determination of 99Tc in soils and solid radioactive wastes using 99mTc as a yield monitor. In the method one and three the samples were leached in low concentrated nitric and sulphuric acid.
Many contaminants were then co-precipitated with Fe(OH)3 in alkali media and Tc in the supernatant was separated using anion-exchange extraction chromatography. There were made also
some studies how to improve the chemical recovery of 99mTc in the process of chromatography. In the method two the sample was ashed and then leached in 8 mol dm−3 HNO3 and after iron precipitation, technetium was separated on chromatographic column. The chemical recovery of 99mTc was optimized in the process of chromatography and leaching. Typical recoveries of technetium determined with 99mTc tracer for all these methods were in the range 39 %–87 %.
The 99Tc activity was measured using proportional low-background beta detector after one week of staying to allow decay of 99mTc activity. 99Tc was also determined by the non-radiometric method using inductively coupled plasma mass spectrometer. 相似文献
14.
The aim of this study is to prepare radiolabeled guanine with 99mTc(CO)3+ core. For this purpose, guanine has been radiolabeled with 99mTc(CO)3+ core. Quality control study of radiolabeled guanine molecule with 99mTc(CO)3+ core was performed by thin layer radio chromatography (TLRC) and high performance liquid radio chromatography (HPLRC). The
results showed that the radiolabeling yield was quite high (94 ± 3%). Beside that 99mTc(CO)3–Gua complex has showed good in vitro stability during the 24 h period. Radiopharmaceutical potential of this complex was
evaluated in Wistar Albino Rats. It was concluded that 99mTc(CO)3–Gua could be used as a nucleotide radiopharmaceutical for in vivo applications. 相似文献
15.
S. Fatahian D. Shahbazi-Gahrouei M. Pouladian M. H. Yousefi Gh. R. Amiri A. Noori 《Journal of Radioanalytical and Nuclear Chemistry》2012,293(3):915-921
DMSA-coated Fe3O4 nanoparticles were synthesized by wet-chemical method. The chemical interaction between Fe3O4 and DMSA were investigated by FTIR. They were directly radiolabeled with 99mTc radioisotope (Fe3O4@DMSA–99mTc) at room temperature in the presence of stannous solution as a reducing agent. Magnetic and structure properties of Fe3O4@DMSA–99mTc nanoparticles were investigated by AGFM, TEM, and XRD. Biodistribution and toxicity assessment of Fe3O4@DMSA–99mTc were studied in mice by intravenous and intraperitoneally injections, respectively. Blood, kidney, and liver factors were measured 4 days post injection and at the mean-while tissue sections were prepared from their kidney and liver. The results indicate that, the Fe3O4@DMSA–99mTc nanoparticles were passed through the membrane of different cells but do not create any disorder in the kidney and liver function even in high doses such as 300 mg/kg. 相似文献
16.
R. Amano 《Journal of Radioanalytical and Nuclear Chemistry》1991,152(1):81-86
Reversed-phase high-performance liquid chromatography with u.v. detection was applied for rapid and sensitive determination of pertechnetate in99Mo/99mTc generator eluates, using a mixture solvent of acetonitrile and 0.04M aqueous acetate buffer (1/1) containing a few volume percentage of 0.5 M tetra-n-butylammonium hydroxide as the mobile phase. Employing a -bondapak C13 column, the TcO
4
–
species was separated, monitored with absorbance at 254 nm, and observed at the retention time of 3.5 min. The detection limit was found to be 5.2·10–10 g of Tc for each injection. Total Tc contents in the99mTc eluates from clinically-used99Mo/99mTc generator were analyzed by this technique. The99mTc (99Tc) species was separated from the contaminant99Mo. This method was found to be useful for the purification of99mTc (99Tc) as well as the determination of total Tc content. 相似文献
17.
Jin-Hwan Kim Sun-Ju Choi Young-Don Hong 《Journal of Radioanalytical and Nuclear Chemistry》2012,292(1):203-209
The organometallic precursor of fac-[99mTc(CO)3(H2O)3]+ has attracted much attention because of the robustness and small size of Tc(I)-tricarbonyl complexes compared to Tc(V) complexes
and the good labeling affinity with a variety of donor atoms. Among various ligand systems, an iminodiacetic acid (IDA) was
proven as a good chelating group to form a Tc(III)-compelx as well as has been shown its potential as a chelating system for
fac-[99mTc(CO)3] precursor. In an attempt to confirm the similarity and the difference between 99mTc(CO)3-IDA and 99mTc-(IDA)2-complex, M(CO)3-IDA (M = 99mTc, Re) complexes of disofenin, mebrofenin and N-(3-iodo-2,4,6-trimethyl phenylcarbamoylmethyl) iminodiacetic acid were prepared, and the biological evaluation of 99mTc(CO)3-disofenin was performed. The 99mTc(CO)3-IDA complexes were prepared with a high radiolabeling yield (>98%) in a quantitative manner and showed a negative charge.
The in vivo pharmacokinetic behavior of 99mTc(CO)3-disofenin showed a similar biological activity to 99mTc-(disofenin)2 in that those complexes were quickly cleared from the blood by the hepatocytes and excreted into the gallbladder and intestine.
Accordingly, the 99mTc(CO)3-IDA derivatives of disofenin and mebrofenin might be used as hepatobiliary imaging agents. Since an IDA is a promising chelator
for 99mTc-based radiopharmaceutical and the biological properties of 99mTc(CO)3-IDA derivative shows similar to that of 99mTc-complex, a biomolecule containing IDA can be freely radiolabeled with fac-[99mTc(CO)3]-precursor or 99mTc. However, the radiolabeling efficiency and the biological behavior demonstrates the favorable properties of 99mTc(CO)3-IDA compound for the development of a new imaging agent. 相似文献
18.
Kakali De Susmita Chandra Bhart Sarkar Santanu Ganguly Mridula Misra 《Journal of Radioanalytical and Nuclear Chemistry》2010,283(3):621-628
In the recent years interests on dihydropyrimidinone and their analogues have increased potentially due to their wide range
of pharmacological/biological activities. Synthesis, radiolabeling with technetium-99 m (99mTc) and biological evaluation of 5-etoxycarbonyl-4-phenyl-6-methyl-3,4-dihydro-(1H)-pyrimidine-2-one (DHPM) were studied in
this present work. After synthesis complexation of DHPM with 99mTc was carried out using stannous chloride as the reducing agent. The complex (99mTc-DHPM) was characterized by thin layer chromatography, radio-HPLC technique and determination of partition co-efficient.
Radiochemical stability and particle size distribution of the complex were also measured. Biodistribution/scintigraphy studies
were performed in rats and rabbits to evaluate the pharmacological characteristics of this complex. The radiochemical purity
of the complex was over 95% as studied by thin layer chromatography and radio-HPLC. It was stable over 24 h at room temperature.
Its partition coefficient indicated that it was a lipophilic complex. According to the European Pharmacopeia, >80% of 99mTc labeled radiopharmaceutical (99mTc-MAA) in the size range 10–50 μm, must be accumulated in the lungs 15 min after intravenous administration. In this study
>85% of the 99mTc-DHPM complex in the average size of 40 μm. Biodistribution studies of 99mTc-DHPM in rat revealed that the complex accumulated in the lung with high uptake and good retention after intravenous administration.
Scintigraphic studies in rabbit also revealed that most of the administered radiolabeled complex was accumulated in the lungs
and after 1 h slowly excreted through the renal system. The lung uptake (ID%/g) was 10.12, 9.67, 8.60 and 5.01 and the lung/liver
ratio was 7.49, 2.88, 2.62 and 1.87 at 2, 15, 30 and 60 min post-injection, respectively. These results suggested that 99mTc-DHPM could be suitable as a potential lung perfusion imaging agent. Further studies with 99mTc-DHPM and its derivatives are warranted to develop new 99mTc-labeled imaging agents for clinical applications. 相似文献
19.
Naoya Harada Masahiro Ono Yoshiro Ohmomo Hidekazu Kawashima 《Journal of organometallic chemistry》2011,696(23):3745-3749
Technetium-99m (99mTc) is one of the most frequently used nuclides for single-photon emission computed tomography (SPECT) imaging because of its radiochemical characteristics, such as gamma emission of suitable energy (141 keV) and adequate half-life (6.01 h). Although triaquatricarbonyl 99mTc cation ([99mTc(CO)3(H2O)3]+) has several advantages as a 99mTc-labeling agent, e.g., compact chelate size, chelate stability, and simplicity of preparation, its synthetic protocols should be improved. Because microwave heating is a convenient method for synthetic reactions, we studied the effect of microwave irradiation on the synthesis of 99mTc tricarbonyl complexes. We found several factors beneficial for the preparation of nuclear medicines. In particular, microwave heating promoted one-pot syntheses of 99mTc tricarbonyl chelates in a short time. In addition, the 99mTc tricarbonyl complex could be obtained using low concentrations of ligands. 相似文献
20.
Rubel Chakravarty Rakesh Shukla Ramu Ram Avesh Kumar Tyagi Ashutosh Dash Meera Venkatesh 《Chromatographia》2010,72(9-10):875-884
The feasibility of using tetragonal nano-zirconia (t-ZrO2) as an effective sorbent for developing a 99Mo/99mTc chromatographic generator was demonstrated. The structural characteristics of the sorbent matrix were investigated by different analytical techniques such as XRD, BET surface area analysis, FT-IR, TEM etc. The material synthesized was nanocrystalline, in tetragonal phase with an average particle size of ~7 nm and a large surface area of 340 m2 g?1. The equilibrium sorption capacity of t-ZrO2 is >250 mg Mo g?1. The present study indicates that 99Mo is both strongly and selectively retained by t-ZrO2 at acidic pH and 99mTc could be readily eluted from it, using 0.9% NaCl solution. A 9.25 GBq (250 mCi) t-ZrO2 based chromatographic 99Mo/99mTc generator was developed and its performance was repeatedly evaluated for 10 days. 99mTc could be eluted with >85% yield having acceptable radionuclidic, radiochemical and chemical purity for clinical applications. The compatibility of the product in the preparation of 99mTc labeled formulations such as 99mTc-EC and 99mTc-DMSA was evaluated and found to be satisfactory. 相似文献