A new type of capillary column for gas chromatography

A new type of capillary column for gas chromatography was proposed. A sorbent layer (for example, stationary liquid phase) is supported on the internal capillary surface, and the internal (interstitial) volume is packed with nonporous large particles of a sorbent (particle diameter is 0.1—0.6 of the capillary internal diameter). The external surface of the particles can also be coated with the sorbent layer (for example, stationary liquid phase). The specific separation efficiency (number of separation) on the new type column is by 1.6—2.3 times higher than that of the initial classical capillary column.     

毛细管电泳在手性分离中的应用进展

本文以手性选择剂为线索综述了近五年来毛细管区带电泳和胶束电动毛细管电色谱在手性药物拆分中的应用进展,列举了部分手性药物拆分实例.     

糖类的毛细管电泳及芯片毛细管电泳

 糖类化合物在生物体内发挥多方面的作用。糖研究的复杂性在于其结构的复杂多变。高效毛细管电泳作为一种快速、高效的分离分析手段已广泛应用于糖的研究。芯片毛细管电泳是近几年来发展起来的新的分析技术 ,并已经在生命科学的研究中得到较广泛的应用。就各种糖类化合物的毛细管电泳的分析策略、检测条件及糖类化合物的芯片毛细管电泳进行了阐述 ,共 4 8篇。     

毛细管电泳涂层柱技术的进展

毛细管电泳涂层柱是解决蛋白质在毛细管壁吸附的最有效的方法。较为系统地综述了毛细管电泳涂层柱的几种制作方法,指出了毛细管电泳涂层柱（包括毛细管电色谱柱）的发展趋势,３９篇。     

使用含离子涂层柱的毛细管电泳和毛细管电色谱的研究进展

 毛细管电色谱是近年发展起来的高效、高选择性的微分离技术。与一般的毛细管电泳和使用ODS反相填料的毛细管电色谱相比 ,含离子涂层柱的毛细管电泳和毛细管电色谱能提供较大且可控的电渗流 ,便于拓宽分离对象 ,优化分离条件。对使用含离子涂层柱的毛细管电泳和电色谱的特点、发展和应用状况进行了综述。     

Cy-5标记脱氧核糖核酸荧光毛细分析法的研究

在荧光毛细分析法（FCA）的基础上开发了一种用于DNA快速检测的DNA-FCA法。在毛细管内表面将DNA探针固定化，制成荧光毛细生物反应器（DNA-CBR）。测定时，用DNA-CBR吸入含Cy-5标记的靶DNA样品液进行杂交反应，然后在646nm激发波长、664nm发射波长下进行荧光测定；Cy-5标记的靶DNA浓度在0．1～1．0μmol／L之间线性良好（y=139．73x＋39．613，r=0．9985）；RSD〈5．5％；检出限为0．17pmol，样品用量10μL；DNA-CBR能够重复使用6次。本方法可用于靶DNA的定性和定最检测。     

3-氨丙基键合开管柱电色谱分离中性芳香族化合物

制备了3-氨丙基键合毛细管开管柱,在此柱上以氨水/甲醇(体积比30∶70)溶液为溶剂,采用毛细管电泳法有效地分离了八种中性芳香族化合物.实验中考察了电色谱条件如:氨水浓度、表面活性剂十二烷基硫酸钠(SDS)浓度及电压等对分离度的影响.结果表明八种中性芳香族化合物在该柱上的分离效果和重现性良好.     

Temperature effects in capillary electrophoresis 2: Some theoretical calculations and predictions

Summary The dependence of the temperature excess, , in the capillary bore on the applied power, EI, is considered for both natural and forced convective cooling, using classical heat equations. The dependence of on EI is found to be linear for forced convection but not for natural convection. Use of forced convective cooling and capillaries of large outer diameter reduces . Direct comparison of the performance of different systems can be achieved by consideration of . Column performance is ultimately limited by thermal gradients across the capillary bore.     

咖啡因及其9种类似物的胶束电动毛细管分析研究

 以十二烷基硫酸钠 (SDS)胶束为准固定相 ,考察了咖啡因及其 9种类似物在胶束电动毛细管 (MECC)分离模式下的分离行为。研究了运行缓冲液的 pH值、磷酸盐浓度、SDS浓度、甲醇体积分数、分离电压、分离温度等因素对这 10种化合物的迁移时间和分离效果的影响。结果发现 ,这些因素对上述 10种化合物的分离有显著的影响 ,尤以pH值为最。它不仅影响化合物的迁移时间和分离效率 ,还改变其出峰顺序 ,这与碱性条件下化合物仲胺基上氢的电离有关。优化后的分离条件 :运行缓冲液为 2 0mmol/L磷酸盐 2 0mmol/LSDS(pH 11 0 ) ,分离电压为2 5kV。     

非水毛细管电泳进展

 毛细管电泳通常是在以水为溶剂的缓冲溶液中进行的,事实上以纯有机溶剂替代水介质同样可以完成特殊样品的电泳分离,且存在诸多优点。以所建立的非水毛细管电泳方法为核心,总结了该方法中有机溶剂、电解质的选择原则及溶质-添加剂相互作用模式,并综述了它在无机离子、中性物质、有机酸等化合物分离分析中的应用。71篇。     

毛细管电色谱柱重复性考察及实验条件的选择

成功地研制了７５μｍ内径偶联式和７５,１００μｍ内径非偶联式毛细管电色谱柱。在自制电色谱柱上获得了小于２．１２％的保留时间ＲＳＤ值,考察了ｐＨ值、有机溶剂浓度对保留行为的影响,并用以指导实验条件的选择。比较了两种方式电色谱柱的峰形。     

Comparison of the separation of aziridine isomers applying heptakis(2,3-di-O-methyl-6-sulfato)beta-CD and heptakis(2,3-di-O-acetyl-6-sulfato)beta-CD in aqueous and nonaqueous systems

Aziridines are attracting interest as protease inhibitors, which might be used, e.g., for treatment of parasitic diseases. Within the framework of greater projects dealing with the search of new selective protease inhibitors, a huge number of aziridines with two stereogenic centers will be synthesized. Thus, a fast and reliable screening method for the evaluation of the isomeric composition is needed. Robust baseline separations were obtained using heptakis(2,3-di-O-acetyl-6-sulfato)beta-CD (HDAS) in acidic methanol and sulfated beta-CD in acidic phosphate buffer. With HDAS the resolutions were higher and migration times shorter. Thus, the method will be used as a screening method for further isomeric mixtures of aziridines.     

亲和毛细管电泳技术及其应用

对近几年新发展起来的亲和毛细管电泳技术（ACE）的原理、分类及方法作了简要介绍,着重介绍了亲和毛细管区带电泳、毛细管亲和凝胶电泳、胶束电动色谱中的亲和电泳、亲和毛细管等电聚焦、亲和探针毛细管电泳等过程和方法。对ACE在分子生物学、生物化学中的应用及该技术在亲和常数测定、核酸片段识别、竞争免疫分析、药物先导化合物的筛选等方面的应用也作了介绍。     

集成毛细管电泳芯片研究进展

 介绍了一种多功能化的快速、高效、高灵敏度和低耗的微型装置集成毛细管电泳芯片的发展和最新研究动向 , 39篇。     

Study of triacontyl-functionalized monolithic silica capillary column for reversed-phase capillary liquid chromatography

A novel stationary phase triacontyl-functionalized monolithic silica capillary column was successfully prepared for reversed-phase capillary liquid chromatography. The performance of the monolithic silica capillary column coated with triacontyl chain for the separation of alkylbenzenes, xylene isomers, polycyclic aromatic hydrocarbons, and mixture of α- and β-carotenes was studied, which was compared to that using the monolithic silica capillary column coated with octadecyl chain. The comparison results showed that triacontyl-functionalized monolithic silica capillary column would be a promising media to be used for the separation of isomeric solutes with long chain in reversed-phase capillary liquid chromatography.     

Application of Capillary Electromigration Methods in the Analysis of Textile Dyes—Review

Fiber traces are one of (micro)traces that can be found at a crime scene. They are easily transferable and, like other forms of evidence, can provide a link between a suspect and a victim. The main purpose of this review is to present methods developed to examine textile dyes extracted for forensic purposes using different capillary electromigration methods (CEMs). Scientific papers, mainly from the 20th century, provide reliable methods for the separation of water-soluble dyes. However, dyes insoluble in aqueous solutions have been and still are a challenge. Another problem is the sensitivity of the developed methods, which is, in most cases, insufficient for forensic examination of dyes extracted from a single fiber preserved at the crime scene. Although the methodologies already developed and presented in this review have the potential to be applied in a comparative analysis of textile dye traces, there seems to be a lot of work to be conducted. Some ideas on how to resolve these problems are presented and discussed in the article.     

Temperature effects in capillary electrophoresis. 1: Internal capillary temperature and effect upon performance

Summary In isothermal CE the migration velocity of analytes and the number of theoretical plates delivered are expected to be proportional to the field strength. In reality ohmic heating of the capillary causes distortions: the migration velocity increases more rapidly while the plate count increases less rapidly, and may even fall at high values of the field. These distortions are worse the larger the bore of the capillary and the higher the concentration of buffer. A detailed investigation of these effects using capillaries cooled by natural convection has confirmed that self heating of the capillary is indeed largely responsible. The extent of self heating has been determined by three independent methods and to a first approximation is proportional to the power dissipation in the capillary. Decreasing viscosity with temperature is responsible for the nonlinearity of the dependence of velocity upon field strength while increase in the diffusion coefficient of analytes is responsible for the poorer than expected performance at high field strengths.     

基于复合型微流控芯片的紫外检测毛细管电泳系统

提出了一种基于芯片-毛细管复合装置的紫外检测-微流控芯片毛细管电泳分析系统.采用小死体积的耦合技术实现了石英毛细管与“十”字通道型微流控玻璃芯片的耦合.本系统的紫外检测灵敏度与商品化毛细管电泳仪相当.采用夹流进样方式,达到较高的进样重现性,2mmol/L苯甲酸的峰高相对标准偏差(RSD)为1.5%(n=11).可用于复方磺胺甲唑片剂的两种有效成分的快速分离.     

高效毛细管电泳I. 分析化学中的一个新的前沿领域

本文是关于高效毛细管电泳(HPCE)的详细综述的第一部分,概括了HPCE技术发展的历史、国内外研究现状,总结了HPCE的基本原理、主要操作方式以及HPCE所具有的特点,并讨论了该技术目前存在的问题及可能的解决方法。