"鸭传染性肿头症"病毒的分离及部分特性研究

成都地区鸭场中流行一种以发病鸭头颈肿胀为特征的传染病,发病率、死亡率均可达到80％以上,各种年龄、品种均易感,危害十分严重,我们通过流行这调查、血凝和血凝抑制试验、中和试验和临床防治试验排除了鸭瘟病毒、禽流感病毒、鸭肝炎病毒、番鸭细小病毒感染的可能性,从典型病例中分离出病毒,在雏鸭上复制出与自然发病相同的病例;电镜下观察了该病毒为圆形或卵圆形,大小为50－70nm,无囊膜;对氯仿有抵抗,对鸭胚的LD50为8．67,无血凝性,初步认为本病是一种新鸭病,暂定名为“鸭传染性肿头症”。     

雏鸭混合感染基因A型和基因C型鸭甲肝病毒研究

研究了2013年四川地区某鸭场暴发的基因A型鸭甲肝病毒(DHAV-A)和基因C型鸭甲肝病毒(DHAV-C)的混合感染病例.疾病发生于20~25日龄雏鸭,发病率25%~30%,死亡率为20%~30%,临死前出现明显的神经症状,病死雏鸭肝脏肿大,出血.利用双重RT-PCR从病死雏鸭的肝脏组织中同时检测出DHAV-A和DHAV-C.这是四川省养鸭密集区第一次发现雏鸭混合感染DHAV-A和DHAV-C的情况.此外,对2009~2012年四川省各养鸭密集区鸭甲肝炎流行情况做了调查.一共从四川省养鸭密集区采集312份疑似鸭肝炎样本中检测出120份鸭甲型肝病毒性肝炎.其中基因C型鸭肝炎阳性率为75%,基因A型鸭肝炎仅为25%,未发现基因A型和基因C型混合感染情况.结果说明2009~2012年我省鸭病毒性肝炎以基因C型鸭甲肝病毒为优势病原.2013年,开始出现DHAV-A和DHAV-C混合感染病例.因此如何采取有效措施应对其危害,这应该引起养鸭界及相关部门的高度重视.     

Host cell apoptosis induced by infection with duck swollen head hemorrhagic disease virus

This study aimed to examine the host cell apoptosis in the tissues of Peking ducks infected with duck swollen head hemorrhagic disease virus (DSHDV). The dynamic changes associated with apoptosis occurring in the internal tissues were evaluated at different time points postinoculation (PI) by performing hematoxylin and eosin (HE) staining, followed by light microscopy, terminal deoxynucleotidyl transfe- rase dUTP nick-end labeling (TUNEL) assay, and transmission electron microscopy (TEM). The results showed that DSHDV infection could induce apoptosis in host cells, including those of the bursa of Fabricius (BF), thymus, spleen, liver, intestinal tract, kidney, and esophagus. The apoptotic index (AI) values increased with time from 2 h to 72 h PI, and the highest values were recorded at 72 h PI. Further, cell death due to classic necrosis was observed in the dying or deceased ducks after 72 h PI. In conclusion, host cell apoptosis can be induced by DSHDV and may play an important role in the pathogenesis of duck viral swollen head hemorrhagic disease (DVSHD).     

鸭肝宁防治雏鸭病毒性肝炎的试验研究

中药口服制剂“鸭肝宁”防治雏鸭病毒性肝炎的结果表明：在实验室攻毒试验只保护率达84％,在临床试用中保护率达85％,仅次于鸭传染性肝炎卵黄抗体的防治水平,具有临床应用价值。     

刈割对"黑土滩"人工草地植被的影响

通过0cm、5cm、10cm和15cm四个不同刈割留茬高度的试验处理,对“黑土滩”上建植的混播人工草地群落结构及生产力进行了研究,结果表明：随着留茬高度的降低,人工草地混播群落中垂穗披碱草和中华羊茅成为减少种,早熟禾则为增加种,西伯利亚蓼成为侵入种;人工草地优良牧草垂穗披碱草和中华羊茅在群落生物量中所占的比例呈下降趋势。因此,适宜的刈割留茬高度可保持“黑土滩”混播人工草地的群落稳定性和草地生产力。     

试论朱淑真诗歌的“个人化”写作立场

与李清照并称隽才的朱淑真,在遭遇北宋灭亡、南宋偏安与自身婚姻不幸的社会苦难和人生苦难的过程中,敢于冲破理学的清规戒律,站在女性性别的立场上用诗歌来表现个人的“私情”,真情吟咏,无所顾忌。她的作品从题材到内容,从风格到情感,都是女性情感生活、家庭生活以及有限的人生梦想的书写,具有极强的个人化色彩和“自我指涉”的私语性特征。     

《国语》不定代词“或”与“莫”

《国语》中不定代词"或"和"莫"的用法有同有异。相同方面:两者在句中都用作主语,这又分为充当小句的主语与充当主谓短语中的主语两种情况。不同方面:不定代词"莫"的出现频率高于不定代词"或",在语法意义上,两者是相反的对应词;就演变趋势而言,《国语》中"莫"已虚化出副词的用法,而"或"还没有虚化的迹象,反映出这组对应词在发展演变中的不同步性。     

新冠肺炎疫情防控过程中的国际科技合作

 根据新型冠状病毒肺炎疫情,分析了国际科技合作在疫情防控中的作用。研究显示,疫情防控涉及国际科技合作多个方面,包括及时交换疫情扩散信息、开展国际间应急联合科研攻关、共享抗病毒药物和医疗技术、人员能力培训和疫情防控体系建设等。从全球治理和国际科技合作的视角,提出了新时期疫情防控的相关政策建议。     

昆德拉和当代中国的"词典"体小说叙事——兼论小说的可能性

作为20世纪的著名小说家之一,昆德拉被看作是小说的“立法者”。他小说的“词典体”因素展示了文学在竞争时代的可能性,反映了时代精神,给中国文学很多启示,并开启了一种“敞开”写作的潮流,显示了小说这一文体的不朽价值。     

谈图书馆“人性化”服务的评估标准及措施

介绍了图书馆“人性化”服务的理念和评估标准,提出了图书馆提高“人性化”服务的具体措施,以便达到逐步完善图书馆的服务功能,充分实现“以人为本”的服务目标。     

Genome sequencing and characterization analysis of a Beijing isolate of chicken coronavirus infectious bronchitis virus

Avian infectious bronchitis virus (AIBV) is classified as a member of the genus coronavirus in the family coronaviridae. The enveloped virus has a positive-sense, single-stranded RNA genome of approximately 28 kilo-bases,which has a 5‘ cap structure and 3‘ polyadenylation tract.The complete genome sequence of infectious bronchitis virus (IBV), Beijing isolate, was determined by cloning sequencing and primer walking. The whole genome is 27733 nucleotides in length, has ten open reading frames:5′-orfla-orflab-s-3a-3b-e-m- 6a-6b-n-3′. Alignments of the genome sequence of IBV Beijing isolate with those of two AIBV strains and one SARS coronavirus were performed respectively. The genome sequence of IBV Beijing isolate compared with that of the IBV strain LX4 (uncompleted, 19440 bp in size) was 91.2% similarity. However, the full-length genome sequence of IBV Beijing isolate was 85.2% identity to that of IBV Strain Beaudette, and was only 50.8% homology to that of SARS coronavirus. The results showed that the genome of IBV has remarkable variation. And IBV Beijing isolate is not closely related to SARS coronavirus. Phylogenetic analyses based on the whole genome sequence, S protein, M protein and N protein, also showed that AIBV Bering isolate is lone virus in group Ⅲ and is distant from SARS coronavirus. In conclusion, this study will contribute to the studies of diagnosis and diseases control on IBV in China.