共查询到20条相似文献,搜索用时 0 毫秒
1.
Yanling Song Yuanzhi Shi Mengjiao Huang Wei Wang Yang Wang Jie Cheng Zhichao Lei Zhi Zhu Chaoyong Yang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(8):2258-2262
Circulating tumor cell (CTC)‐enrichment by using aptamers has a number of advantages, but the issue of compromised binding affinities and stabilities in real samples hinders its wide applications. Inspired by the high efficiency of the prey mechanism of the octopus, we engineered a deterministic lateral displacement (DLD)‐patterned microfluidic chip modified with multivalent aptamer‐functionalized nanospheres (AP‐Octopus‐Chip) to enhance capture efficiency. The multivalent aptamer–antigen binding efficiency improves 100‐fold and the capture efficiency is enhanced more than 300 % compared with a monovalent aptamer‐modified chip. Moreover, the captured cancer cells can be released through a thiol exchange reaction with up to 80 % efficiency and 96 % viability, which is fully compatible with downstream mutation detection and CTC culture. Using the chip, we were able to find CTCs in all cancer samples analyzed. 相似文献
2.
3.
4.
《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2017,129(36):10821-10825
Even though the diagnostic and prognostic value of circulating tumor cells (CTCs) has been demonstrated, their clinical utility and widespread adoption have been limited. Herein, we describe a new device, size‐dictated immunocapture chip (SDI‐Chip), for efficient, sensitive, and spatially resolved capture and detection of CTCs. SDI‐Chip enables selective, frequent, and extended interaction of CTCs with hydrodynamically optimized immunocoated micropillar surfaces. CTCs with different antigen expression levels can be efficiently captured and spatially resolved around the micropillars. Capture efficiency greater than 92 % with a purity of 82 % was achieved with blood samples. CTCs were detected in non‐metastasis colorectal (CRC) patients, while none was detected from healthy volunteers. We believe that SDI‐Chip will facilitate the transition of tumor diagnosis from anatomical pathology to molecular pathology in localized CRC patients. 相似文献
5.
Shutao Wang Kan Liu Jian Liu Zeta T.‐F. Yu Xiaowen Xu Libo Zhao Tom Lee Eun Kyung Lee Jean Reiss Yi‐Kuen Lee Leland W. K. Chung Jiaoti Huang Matthew Rettig David Seligson Kumaran N. Duraiswamy Clifton K.‐F. Shen Hsian‐Rong Tseng 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2011,123(13):2909-2909
6.
7.
8.
9.
Xiaomin Ren Miao Guo He Li Chengbin Li Liang Yu Jian Liu Qihua Yang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(41):14530-14530
10.
11.
12.
13.
Hanhan Guo Xiaorong Song Wen Lei Cheng He Wenwu You Qingzhong Lin Shanyong Zhou Xueyuan Chen Zhuo Chen 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(35):12323-12327
The detection of circulating tumor cells (CTCs) is crucial to early cancer diagnosis and the evaluation of cancer metastasis. However, it remains challenging due to the scarcity of CTCs in the blood. Herein, we report an ultrasensitive platform for the direct detection of CTCs using luminescent lanthanide nanoprobes. These were designed to recognize the epithelial cell adhesion molecules on cancer cells, allowing signal amplification through dissolution‐enhanced time‐resolved photoluminescence (TRPL) and the elimination of short‐lived autofluorescence interference. This enabled the direct detection of blood breast‐cancer cells with a limit of detection down to 1 cell/well of a 96‐well plate. Moreover, blood CTCs (≥10 cells mL?1) can be detected in cancer patients with a detection rate of 93.9 % (14/15 patients). We envision that this ultrasensitive detection platform with excellent practicality may provide an effective strategy for early cancer diagnosis and prognosis evaluation. 相似文献
14.
15.
16.
17.
18.
19.