共查询到20条相似文献,搜索用时 15 毫秒
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Zuhai Lei Caixia Sun Peng Pei Shangfeng Wang Dandan Li Xin Zhang Fan Zhang 《Angewandte Chemie (International ed. in English)》2019,58(24):8166-8171
Small‐molecule organic fluorophores, spectrally active in the 900–1700 nm region, with tunable wavelength and sensing properties are sought‐after for in vivo optical imaging and biosensing. A panel of fluorescent dyes ( CX ) has been developed to meet this challenge. CX dyes exhibit the wavelength tunability of cyanine dyes and have a rigidified polymethine chain to guarantee their stability. They are chemo‐ and photo‐stable in an aqueous environment and have tunable optical properties with maximal absorbing/emitting wavelength at 1089/1140 nm. They show great potential in high‐contrast in vivo bioimaging and multicolor detection with negligible optical cross talk. Förster resonance energy transfer (FRET) between CX dyes was demonstrated in deep tissue, providing an approach for monitoring drug‐induced hepatotoxicity by detection of OONO?. This report presents a series of NIR‐II dyes with promising spectroscopic properties for high‐contrast bioimaging and multiplexed biosensing. 相似文献
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《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2017,129(10):2680-2683
Limitations on the number of proteins that can be quantified in single cells in situ impede advances in our deep understanding of normal cell physiology and disease pathogenesis. Herein, we present a highly multiplexed single‐cell in situ protein analysis approach that is based on chemically cleavable fluorescent antibodies. In this method, antibodies tethered to fluorophores through a novel azide‐based cleavable linker are utilized to detect their protein targets. After fluorescence imaging and data storage, the fluorophores coupled to the antibodies are efficiently cleaved without loss of protein target antigenicity. Upon continuous cycles of target recognition, fluorescence imaging, and fluorophore cleavage, this approach has the potential to quantify over 100 different proteins in individual cells at optical resolution. This single‐cell in situ protein profiling technology will have wide applications in signaling network analysis, molecular diagnosis, and cellular targeted therapies. 相似文献
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Hongxin Zhang Yong Fan Peng Pei Caixia Sun Lingfei Lu Fan Zhang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(30):10259-10263
In vivo fluorescence imaging in the second near‐infrared window (NIR‐II) affords deep‐tissue penetration and high spatial resolution. Herein, we present a new type of Tm3+‐sensitized lanthanide nanocrystals with both excitation (1208 nm) and emission (1525 nm) located in the NIR‐II window for in vivo optical information storage and decoding. Taking advantage of the tunable fluorescence lifetimes, the optical multiplexed encoding capacity is enhanced accordingly. Micro‐devices with QR codes featuring the NIR‐II fluorescence‐lifetime multiplexed encoding were implanted into mice and were successfully decoded through time‐gated fluorescence imaging technology. 相似文献
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Koji Numasawa Kenjiro Hanaoka Naoko Saito Yoshifumi Yamaguchi Takayuki Ikeno Honami Echizen Masahiro Yasunaga Toru Komatsu Tasuku Ueno Masayuki Miura Tetsuo Nagano Yasuteru Urano 《Angewandte Chemie (International ed. in English)》2020,59(15):6015-6020
Folate receptors (FRs) are membrane proteins involved in folic acid uptake, and the alpha isoform (FR‐α) is overexpressed in ovarian and endometrial cancer cells. For fluorescence imaging of FRs in vivo, the near‐infrared (NIR) region (650–900 nm), in which tissue penetration is high and autofluorescence is low, is optimal, but existing NIR fluorescent probes targeting FR‐α show high non‐specific tissue adsorption, and require prolonged washout to visualize tumors. We have designed and synthesized a new NIR fluorescent probe, FolateSiR‐1 , utilizing a Si‐rhodamine fluorophore having a carboxy group at the benzene moiety, coupled to a folate ligand moiety through a negatively charged tripeptide linker. This probe exhibits very low background fluorescence and afforded a tumor‐to‐background ratio (TBR) of up to 83 in FR‐expressing tumor‐bearing mice within 30 min. Thus, FolateSiR‐1 has the potential to contribute to the research in the field of biology and the clinical medicine. 相似文献
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Chunyan Li Wanfei Li Huanhuan Liu Yejun Zhang Guangcun Chen Zijing Li Qiangbin Wang 《Angewandte Chemie (International ed. in English)》2020,59(1):247-252
Traumatic brain injury (TBI) is one of the most dangerous acute diseases resulting in high morbidity and mortality. Current methods remain limited with respect to early diagnosis and real‐time feedback on the pathological process. Herein, a targeted activatable fluorescent nanoprobe (V&A@Ag2S) in the second near‐infrared window (NIR‐II) is presented for in vivo optical imaging of TBI. Initially, the fluorescence of V&A@Ag2S is turned off owing to energy transfer from Ag2S to the A1094 chromophore. Upon intravenous injection, V&A@Ag2S quickly accumulates in the inflamed vascular endothelium of TBI based on VCAM1‐mediated endocytosis, after which the nanoprobe achieves rapid recovery of the NIR‐II fluorescence of Ag2S quantum dots (QDs) owing to the bleaching of A1094 by the prodromal biomarker of TBI, peroxynitrite (ONOO?). The nanoprobe offers high specificity, rapid response, and high sensitivity toward ONOO?, providing a convenient approach for in vivo early real‐time assessment of TBI. 相似文献
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Mengyao Zhao Rui Wang Benhao Li Yong Fan Yifan Wu Xinyan Zhu Fan Zhang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(7):2072-2076
To improve the bioimaging signal‐to‐noise ratio (SNR), long‐term imaging capability, and decrease the potential biotoxicity, an in vivo cross‐linking strategy was developed by using sub‐10 nm, glutathione‐modified, lanthanide nanoprobes. After administration, the nanoprobes cross‐link in response to reactive oxygen species (ROS) at the inflamed area and enable the quick imaging of ROS in the second near‐infrared (NIR‐II) window. These nanoprobes could be rapidly excreted due to their ultra‐small size. This strategy may also be applied to other ultra‐small contrast agents for the precise bioimaging by in situ lesion cross‐linking. 相似文献
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Chenge Li Alison G. Tebo Marion Thauvin Marie‐Aude Plamont Michel Volovitch Xavier Morin Sophie Vriz Arnaud Gautier 《Angewandte Chemie (International ed. in English)》2020,59(41):17917-17923
Far‐red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far‐red Fluorescence Activating and absorption Shifting Tag), a 14‐kDa monomeric protein that forms a bright far‐red fluorescent assembly with (4‐hydroxy‐3‐methoxy‐phenyl)allylidene rhodanine (HPAR‐3OM). As HPAR‐3OM is essentially non‐fluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR‐3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae, and chicken embryos. Beyond enabling the genetic encoding of far‐red fluorescence, frFAST allowed the design of a far‐red chemogenetic reporter of protein–protein interactions, demonstrating its great potential for the design of innovative far‐red emitting biosensors. 相似文献
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Haibin Xiao Wen Zhang Ping Li Wei Zhang Xin Wang Bo Tang 《Angewandte Chemie (International ed. in English)》2020,59(11):4216-4230
The superoxide anion (O2.?) is widely engaged in the regulation of cell functions and is thereby intimately associated with the onset and progression of many diseases. To ascertain the pathological roles of O2.? in related diseases, developing effective methods for monitoring O2.? in biological systems is essential. Fluorescence imaging is a powerful tool for monitoring bioactive molecules in cells and in vivo owing to its high sensitivity and high temporal‐spatial resolution. Therefore, increasing numbers of fluorescent imaging probes have been constructed to monitor O2.? inside live cells and small animals. In this minireview, we summarize the methods for design and application of O2.?‐responsive fluorescent probes. Moreover, we present the challenges for detecting O2.? and suggestions for constructing new fluorescent probes that can indicate the production sites and concentration changes in O2.? as well as O2.?‐associated active molecules in living cells and in vivo. 相似文献
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Subin Son Miae Won Ori Green Nir Hananya Amit Sharma Yukyoung Jeon Jong Hwan Kwak Jonathan L. Sessler Doron Shabat Jong Seung Kim 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(6):1753-1757
Activatable (turn‐on) probes that permit the rapid, sensitive, selective, and accurate identification of cancer‐associated biomarkers can help drive advances in cancer research. Herein, a NAD(P)H:quinone oxidoreductase‐1 (NQO1)‐specific chemiluminescent probe 1 is reported that allows the differentiation between cancer subtypes. Probe 1 incorporates an NQO1‐specific trimethyl‐locked quinone trigger moiety covalently tethered to a phenoxy‐dioxetane moiety through a para‐aminobenzyl alcohol linker. Bio‐reduction of the quinone to the corresponding hydroquinone results in a chemiluminescent signal. As inferred from a combination of in vitro cell culture analyses and in vivo mice studies, the probe is safe, cell permeable, and capable of producing a “turn‐on” luminescence response in an NQO1‐positive A549 lung cancer model. On this basis, probe 1 can be used to identify cancerous cells and tissues characterized by elevated NQO1 levels. 相似文献
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Changkui Fu Baris Demir Sheilajen Alcantara Vinod Kumar Felicity Han Hannah G. Kelly Xiao Tan Ye Yu Weizhi Xu Jiacheng Zhao Cheng Zhang Hui Peng Cyrille Boyer Trent M. Woodruff Stephen J. Kent Debra J. Searles Andrew K. Whittaker 《Angewandte Chemie (International ed. in English)》2020,59(12):4729-4735
The conjugation of hydrophilic low‐fouling polymers to therapeutic molecules and particles is an effective approach to improving their aqueous stability, solubility, and pharmacokinetics. Recent concerns over the immunogenicity of poly(ethylene glycol) has highlighted the importance of identifying alternative low fouling polymers. Now, a new class of synthetic water‐soluble homo‐fluoropolymers are reported with a sulfoxide side‐chain structure. The incorporation of fluorine enables direct imaging of the homopolymer by 19F MRI, negating the need for additional synthetic steps to attach an imaging moiety. These self‐reporting fluoropolymers show outstanding imaging sensitivity and remarkable hydrophilicity, and as such are a new class of low‐fouling polymer for bioconjugation and in vivo tracking. 相似文献
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o‐Fluorination of Aromatic Azides Yields Improved Azido‐Based Fluorescent Probes for Hydrogen Sulfide: Synthesis,Spectra, and Bioimaging 下载免费PDF全文
Chao Wei Runyu Wang Dr. Lv Wei Longhuai Cheng Zhifei Li Prof. Dr. Zhen Xi Prof. Dr. Long Yi 《化学:亚洲杂志》2014,9(12):3586-3592
Hydrogen sulfide (H2S) is an endogenously produced gaseous signaling molecule with multiple biological functions. To visualize the endogenous in situ production of H2S in real time, new coumarin‐ and boron‐dipyrromethene‐based fluorescent turn‐on probes were developed for fast sensing of H2S in aqueous buffer and in living cells. Introduction of a fluoro group in the ortho position of the aromatic azide can lead to a greater than twofold increase in the rate of reaction with H2S. On the basis of o‐fluorinated aromatic azides, fluorescent probes with high sensitivity and selectivity toward H2S over other biologically relevant species were designed and synthesized. The probes can be used to in situ to visualize exogenous H2S and D ‐cysteine‐dependent endogenously produced H2S in living cells, which makes them promising tools for potential applications in H2S biology. 相似文献
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Sam Benson Antonio Fernandez Nicole D. Barth Fabio deMoliner Mathew H. Horrocks C. Simon Herrington Jose Luis Abad Antonio Delgado Lisa Kelly Ziyuan Chang Yi Feng Miyako Nishiura Yuichiro Hori Kazuya Kikuchi Marc Vendrell 《Angewandte Chemie (International ed. in English)》2019,58(21):6911-6915
The transport and trafficking of metabolites are critical for the correct functioning of live cells. However, in situ metabolic imaging studies are hampered by the lack of fluorescent chemical structures that allow direct monitoring of small metabolites under physiological conditions with high spatial and temporal resolution. Herein, we describe SCOTfluors as novel small‐sized multi‐colored fluorophores for real‐time tracking of essential metabolites in live cells and in vivo and for the acquisition of metabolic profiles from human cancer cells of variable origin. 相似文献